Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS15755 and EX31_RS15760 are separated by 22 nucleotides EX31_RS15760 and EX31_RS15765 are separated by 236 nucleotides EX31_RS15765 and EX31_RS15770 are separated by 52 nucleotides
EX31_RS15755: EX31_RS15755 - hypothetical protein, at 2,847 to 3,674
_RS15755
EX31_RS15760: EX31_RS15760 - hypothetical protein, at 3,697 to 4,281
_RS15760
EX31_RS15765: EX31_RS15765 - helix-turn-helix transcriptional regulator, at 4,518 to 4,895
_RS15765
EX31_RS15770: EX31_RS15770 - hypothetical protein, at 4,948 to 5,529
_RS15770
Position (kb) 3
4
5 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 2.830 kb on + strand at 2.833 kb on - strand at 2.875 kb on + strand at 2.905 kb on - strand at 2.946 kb on - strand, within EX31_RS15755 at 3.137 kb on - strand, within EX31_RS15755 at 3.263 kb on + strand, within EX31_RS15755 at 3.283 kb on - strand, within EX31_RS15755 at 3.300 kb on - strand, within EX31_RS15755 at 3.303 kb on + strand, within EX31_RS15755 at 3.305 kb on + strand, within EX31_RS15755 at 3.320 kb on + strand, within EX31_RS15755 at 3.321 kb on - strand, within EX31_RS15755 at 3.389 kb on - strand, within EX31_RS15755 at 3.402 kb on - strand, within EX31_RS15755 at 3.502 kb on - strand, within EX31_RS15755 at 3.506 kb on + strand, within EX31_RS15755 at 3.509 kb on - strand, within EX31_RS15755 at 3.545 kb on + strand, within EX31_RS15755 at 3.610 kb on + strand at 3.611 kb on - strand at 3.732 kb on - strand at 3.736 kb on - strand at 3.743 kb on - strand at 3.743 kb on - strand at 3.744 kb on + strand at 3.744 kb on + strand at 3.744 kb on + strand at 3.744 kb on + strand at 3.745 kb on - strand at 3.745 kb on - strand at 3.745 kb on - strand at 3.747 kb on - strand at 3.766 kb on - strand, within EX31_RS15760 at 3.812 kb on - strand, within EX31_RS15760 at 3.812 kb on - strand, within EX31_RS15760 at 3.830 kb on - strand, within EX31_RS15760 at 3.830 kb on - strand, within EX31_RS15760 at 3.886 kb on - strand, within EX31_RS15760 at 3.981 kb on + strand, within EX31_RS15760 at 3.981 kb on + strand, within EX31_RS15760 at 3.987 kb on + strand, within EX31_RS15760 at 3.987 kb on + strand, within EX31_RS15760 at 3.997 kb on + strand, within EX31_RS15760 at 4.083 kb on + strand, within EX31_RS15760 at 4.087 kb on - strand, within EX31_RS15760 at 4.087 kb on - strand, within EX31_RS15760 at 4.087 kb on - strand, within EX31_RS15760 at 4.100 kb on - strand, within EX31_RS15760 at 4.206 kb on + strand, within EX31_RS15760 at 4.229 kb on + strand at 4.230 kb on - strand at 4.230 kb on - strand at 4.230 kb on - strand at 4.230 kb on - strand at 4.231 kb on + strand at 4.231 kb on + strand at 4.231 kb on + strand at 4.231 kb on + strand at 4.231 kb on + strand at 4.232 kb on - strand at 4.232 kb on - strand at 4.232 kb on - strand at 4.234 kb on - strand at 4.257 kb on + strand at 4.258 kb on - strand at 4.260 kb on + strand at 4.260 kb on + strand at 4.260 kb on + strand at 4.261 kb on - strand at 4.261 kb on - strand at 4.261 kb on - strand at 4.264 kb on + strand at 4.311 kb on - strand at 4.328 kb on - strand at 4.386 kb on + strand at 4.414 kb on + strand at 4.435 kb on - strand at 4.435 kb on - strand at 4.435 kb on - strand at 4.457 kb on - strand at 4.487 kb on + strand at 4.488 kb on - strand at 4.488 kb on - strand at 4.497 kb on + strand at 4.497 kb on + strand at 4.498 kb on - strand at 4.515 kb on + strand at 4.515 kb on + strand at 4.516 kb on - strand at 4.528 kb on + strand at 4.528 kb on + strand at 4.528 kb on + strand at 4.529 kb on - strand at 4.541 kb on + strand at 4.541 kb on + strand at 4.541 kb on + strand at 4.547 kb on + strand at 4.548 kb on - strand at 4.548 kb on - strand at 4.548 kb on - strand at 4.565 kb on + strand, within EX31_RS15765 at 4.565 kb on + strand, within EX31_RS15765 at 4.565 kb on + strand, within EX31_RS15765 at 4.565 kb on + strand, within EX31_RS15765 at 4.565 kb on + strand, within EX31_RS15765 at 4.565 kb on + strand, within EX31_RS15765 at 4.565 kb on + strand, within EX31_RS15765 at 4.565 kb on + strand, within EX31_RS15765 at 4.565 kb on + strand, within EX31_RS15765 at 4.565 kb on + strand, within EX31_RS15765 at 4.565 kb on + strand, within EX31_RS15765 at 4.565 kb on + strand, within EX31_RS15765 at 4.565 kb on + strand, within EX31_RS15765 at 4.565 kb on + strand, within EX31_RS15765 at 4.565 kb on + strand, within EX31_RS15765 at 4.566 kb on - strand, within EX31_RS15765 at 4.566 kb on - strand, within EX31_RS15765 at 4.566 kb on - strand, within EX31_RS15765 at 4.566 kb on - strand, within EX31_RS15765 at 4.566 kb on - strand, within EX31_RS15765 at 4.566 kb on - strand, within EX31_RS15765 at 4.566 kb on - strand, within EX31_RS15765 at 4.566 kb on - strand, within EX31_RS15765 at 4.566 kb on - strand, within EX31_RS15765 at 4.566 kb on - strand, within EX31_RS15765 at 4.566 kb on - strand, within EX31_RS15765 at 4.566 kb on - strand, within EX31_RS15765 at 4.566 kb on - strand, within EX31_RS15765 at 4.566 kb on - strand, within EX31_RS15765 at 4.566 kb on - strand, within EX31_RS15765 at 4.566 kb on - strand, within EX31_RS15765 at 4.566 kb on - strand, within EX31_RS15765 at 4.566 kb on - strand, within EX31_RS15765 at 4.567 kb on + strand, within EX31_RS15765 at 4.567 kb on + strand, within EX31_RS15765 at 4.568 kb on - strand, within EX31_RS15765 at 4.568 kb on - strand, within EX31_RS15765 at 4.605 kb on + strand, within EX31_RS15765 at 4.605 kb on + strand, within EX31_RS15765 at 4.625 kb on + strand, within EX31_RS15765 at 4.625 kb on + strand, within EX31_RS15765 at 4.625 kb on + strand, within EX31_RS15765 at 4.664 kb on + strand, within EX31_RS15765 at 4.668 kb on - strand, within EX31_RS15765 at 4.668 kb on - strand, within EX31_RS15765 at 4.704 kb on - strand, within EX31_RS15765 at 4.734 kb on - strand, within EX31_RS15765 at 4.753 kb on + strand, within EX31_RS15765 at 4.754 kb on - strand, within EX31_RS15765 at 4.754 kb on - strand, within EX31_RS15765 at 4.754 kb on - strand, within EX31_RS15765 at 4.936 kb on - strand at 4.939 kb on - strand at 5.036 kb on - strand, within EX31_RS15770 at 5.047 kb on - strand, within EX31_RS15770 at 5.126 kb on + strand, within EX31_RS15770 at 5.127 kb on - strand, within EX31_RS15770 at 5.144 kb on + strand, within EX31_RS15770 at 5.148 kb on + strand, within EX31_RS15770 at 5.148 kb on + strand, within EX31_RS15770 at 5.149 kb on - strand, within EX31_RS15770 at 5.149 kb on - strand, within EX31_RS15770 at 5.150 kb on + strand, within EX31_RS15770 at 5.151 kb on - strand, within EX31_RS15770 at 5.151 kb on - strand, within EX31_RS15770 at 5.151 kb on - strand, within EX31_RS15770 at 5.153 kb on - strand, within EX31_RS15770 at 5.153 kb on - strand, within EX31_RS15770 at 5.158 kb on + strand, within EX31_RS15770 at 5.193 kb on - strand, within EX31_RS15770 at 5.268 kb on + strand, within EX31_RS15770 at 5.274 kb on + strand, within EX31_RS15770
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 2,830 + +1.4 2,833 - -0.3 2,875 + -0.7 2,905 - +0.4 2,946 - EX31_RS15755 0.12 -0.2 3,137 - EX31_RS15755 0.35 -0.4 3,263 + EX31_RS15755 0.50 +0.7 3,283 - EX31_RS15755 0.53 +0.9 3,300 - EX31_RS15755 0.55 -0.7 3,303 + EX31_RS15755 0.55 -0.6 3,305 + EX31_RS15755 0.55 +2.4 3,320 + EX31_RS15755 0.57 -0.0 3,321 - EX31_RS15755 0.57 +0.5 3,389 - EX31_RS15755 0.65 +0.8 3,402 - EX31_RS15755 0.67 +0.4 3,502 - EX31_RS15755 0.79 -0.4 3,506 + EX31_RS15755 0.80 -0.0 3,509 - EX31_RS15755 0.80 -0.9 3,545 + EX31_RS15755 0.84 +0.0 3,610 + +0.1 3,611 - +0.1 3,732 - +0.9 3,736 - +1.3 3,743 - +0.1 3,743 - +0.0 3,744 + +0.5 3,744 + +0.3 3,744 + +0.9 3,744 + +0.5 3,745 - -0.6 3,745 - +0.4 3,745 - +0.7 3,747 - +1.0 3,766 - EX31_RS15760 0.12 +1.1 3,812 - EX31_RS15760 0.20 +0.7 3,812 - EX31_RS15760 0.20 -0.0 3,830 - EX31_RS15760 0.23 -2.1 3,830 - EX31_RS15760 0.23 +0.3 3,886 - EX31_RS15760 0.32 -0.2 3,981 + EX31_RS15760 0.49 +0.4 3,981 + EX31_RS15760 0.49 -0.7 3,987 + EX31_RS15760 0.50 -0.1 3,987 + EX31_RS15760 0.50 -0.0 3,997 + EX31_RS15760 0.51 +0.8 4,083 + EX31_RS15760 0.66 -0.1 4,087 - EX31_RS15760 0.67 -1.0 4,087 - EX31_RS15760 0.67 +0.6 4,087 - EX31_RS15760 0.67 -0.4 4,100 - EX31_RS15760 0.69 +1.2 4,206 + EX31_RS15760 0.87 +0.4 4,229 + +0.9 4,230 - -1.0 4,230 - +0.6 4,230 - +0.2 4,230 - -0.3 4,231 + +0.4 4,231 + +0.6 4,231 + -0.7 4,231 + -0.1 4,231 + +1.0 4,232 - +0.1 4,232 - +0.7 4,232 - +0.4 4,234 - +0.4 4,257 + +0.7 4,258 - +0.8 4,260 + +0.7 4,260 + +2.4 4,260 + -0.3 4,261 - -1.1 4,261 - +0.7 4,261 - +0.2 4,264 + -0.5 4,311 - -0.1 4,328 - -0.4 4,386 + -1.0 4,414 + +1.4 4,435 - -1.8 4,435 - +0.1 4,435 - -0.1 4,457 - +0.3 4,487 + -0.5 4,488 - -0.9 4,488 - +1.6 4,497 + +0.6 4,497 + -0.4 4,498 - +0.5 4,515 + +0.1 4,515 + +0.5 4,516 - -0.2 4,528 + +0.4 4,528 + +0.4 4,528 + +0.6 4,529 - +0.5 4,541 + +0.6 4,541 + -0.2 4,541 + -0.7 4,547 + -0.1 4,548 - -0.0 4,548 - +1.6 4,548 - -0.0 4,565 + EX31_RS15765 0.12 -0.1 4,565 + EX31_RS15765 0.12 -0.8 4,565 + EX31_RS15765 0.12 -0.9 4,565 + EX31_RS15765 0.12 -0.6 4,565 + EX31_RS15765 0.12 +0.9 4,565 + EX31_RS15765 0.12 -0.8 4,565 + EX31_RS15765 0.12 -0.1 4,565 + EX31_RS15765 0.12 +0.8 4,565 + EX31_RS15765 0.12 -0.1 4,565 + EX31_RS15765 0.12 +1.3 4,565 + EX31_RS15765 0.12 -0.5 4,565 + EX31_RS15765 0.12 -1.1 4,565 + EX31_RS15765 0.12 -0.6 4,565 + EX31_RS15765 0.12 -0.4 4,565 + EX31_RS15765 0.12 +0.4 4,566 - EX31_RS15765 0.13 -0.3 4,566 - EX31_RS15765 0.13 -0.3 4,566 - EX31_RS15765 0.13 +1.8 4,566 - EX31_RS15765 0.13 -2.8 4,566 - EX31_RS15765 0.13 +0.3 4,566 - EX31_RS15765 0.13 -0.4 4,566 - EX31_RS15765 0.13 -0.3 4,566 - EX31_RS15765 0.13 -0.0 4,566 - EX31_RS15765 0.13 +0.8 4,566 - EX31_RS15765 0.13 +0.0 4,566 - EX31_RS15765 0.13 +0.4 4,566 - EX31_RS15765 0.13 -2.4 4,566 - EX31_RS15765 0.13 -0.6 4,566 - EX31_RS15765 0.13 -0.9 4,566 - EX31_RS15765 0.13 -0.0 4,566 - EX31_RS15765 0.13 -0.5 4,566 - EX31_RS15765 0.13 +0.6 4,566 - EX31_RS15765 0.13 -1.0 4,567 + EX31_RS15765 0.13 -0.2 4,567 + EX31_RS15765 0.13 -0.1 4,568 - EX31_RS15765 0.13 +0.5 4,568 - EX31_RS15765 0.13 +0.0 4,605 + EX31_RS15765 0.23 -0.6 4,605 + EX31_RS15765 0.23 +0.0 4,625 + EX31_RS15765 0.28 +0.6 4,625 + EX31_RS15765 0.28 +0.1 4,625 + EX31_RS15765 0.28 -1.1 4,664 + EX31_RS15765 0.39 +0.3 4,668 - EX31_RS15765 0.40 +0.4 4,668 - EX31_RS15765 0.40 -0.5 4,704 - EX31_RS15765 0.49 +0.6 4,734 - EX31_RS15765 0.57 +0.2 4,753 + EX31_RS15765 0.62 +0.1 4,754 - EX31_RS15765 0.62 -0.9 4,754 - EX31_RS15765 0.62 +0.7 4,754 - EX31_RS15765 0.62 -0.1 4,936 - -0.5 4,939 - +0.8 5,036 - EX31_RS15770 0.15 +0.3 5,047 - EX31_RS15770 0.17 +0.4 5,126 + EX31_RS15770 0.31 +1.3 5,127 - EX31_RS15770 0.31 +0.0 5,144 + EX31_RS15770 0.34 +0.4 5,148 + EX31_RS15770 0.34 -0.1 5,148 + EX31_RS15770 0.34 -0.1 5,149 - EX31_RS15770 0.35 +0.5 5,149 - EX31_RS15770 0.35 +1.2 5,150 + EX31_RS15770 0.35 -0.0 5,151 - EX31_RS15770 0.35 +0.1 5,151 - EX31_RS15770 0.35 +0.3 5,151 - EX31_RS15770 0.35 +1.1 5,153 - EX31_RS15770 0.35 -0.2 5,153 - EX31_RS15770 0.35 -0.6 5,158 + EX31_RS15770 0.36 -0.5 5,193 - EX31_RS15770 0.42 -0.2 5,268 + EX31_RS15770 0.55 -0.4 5,274 + EX31_RS15770 0.56 -1.0
Or see this region's nucleotide sequence