Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS14630 and EX31_RS14635 are separated by 157 nucleotides EX31_RS14635 and EX31_RS14640 are separated by 165 nucleotides EX31_RS14640 and EX31_RS14645 are separated by 223 nucleotides
EX31_RS14630: EX31_RS14630 - DNA recombination protein RmuC, at 17,865 to 19,406
_RS14630
EX31_RS14635: EX31_RS14635 - DedA family protein, at 19,564 to 20,091
_RS14635
EX31_RS14640: EX31_RS14640 - uridine phosphorylase, at 20,257 to 21,018
_RS14640
EX31_RS14645: EX31_RS14645 - catalase HPII, at 21,242 to 23,503
_RS14645
Position (kb) 20
21
22 Strain fitness (log2 ratio) -2
-1
0
1
2 at 19.263 kb on - strand at 19.263 kb on - strand at 19.306 kb on + strand at 19.354 kb on - strand at 19.359 kb on + strand at 19.360 kb on - strand at 19.360 kb on - strand at 19.441 kb on + strand at 19.442 kb on - strand at 19.442 kb on - strand at 19.524 kb on + strand at 19.524 kb on + strand at 19.525 kb on - strand at 19.525 kb on - strand at 19.853 kb on + strand, within EX31_RS14635 at 19.854 kb on - strand, within EX31_RS14635 at 19.957 kb on - strand, within EX31_RS14635 at 19.957 kb on - strand, within EX31_RS14635 at 19.957 kb on - strand, within EX31_RS14635 at 19.957 kb on - strand, within EX31_RS14635 at 20.058 kb on + strand at 20.058 kb on + strand at 20.059 kb on - strand at 20.059 kb on - strand at 20.059 kb on - strand at 20.059 kb on - strand at 20.066 kb on + strand at 20.137 kb on + strand at 20.138 kb on - strand at 20.151 kb on + strand at 20.151 kb on + strand at 20.151 kb on + strand at 20.151 kb on + strand at 20.152 kb on - strand at 20.154 kb on + strand at 20.154 kb on + strand at 20.154 kb on + strand at 20.155 kb on - strand at 20.178 kb on + strand at 20.243 kb on + strand at 20.243 kb on + strand at 20.249 kb on + strand at 20.250 kb on - strand at 20.250 kb on - strand at 20.250 kb on - strand at 20.251 kb on + strand at 20.258 kb on + strand at 20.258 kb on + strand at 20.258 kb on + strand at 20.258 kb on + strand at 20.259 kb on - strand at 20.259 kb on - strand at 20.259 kb on - strand at 20.262 kb on + strand at 20.262 kb on + strand at 20.262 kb on + strand at 20.262 kb on + strand at 20.262 kb on + strand at 20.263 kb on - strand at 20.263 kb on - strand at 20.263 kb on - strand at 20.281 kb on + strand at 20.282 kb on - strand at 20.282 kb on - strand at 20.435 kb on + strand, within EX31_RS14640 at 20.436 kb on - strand, within EX31_RS14640 at 20.504 kb on + strand, within EX31_RS14640 at 20.504 kb on + strand, within EX31_RS14640 at 20.504 kb on + strand, within EX31_RS14640 at 20.505 kb on - strand, within EX31_RS14640 at 20.505 kb on - strand, within EX31_RS14640 at 20.505 kb on - strand, within EX31_RS14640 at 20.505 kb on - strand, within EX31_RS14640 at 20.505 kb on - strand, within EX31_RS14640 at 20.505 kb on - strand, within EX31_RS14640 at 20.514 kb on - strand, within EX31_RS14640 at 20.531 kb on + strand, within EX31_RS14640 at 20.757 kb on + strand, within EX31_RS14640 at 20.757 kb on + strand, within EX31_RS14640 at 20.757 kb on + strand, within EX31_RS14640 at 20.757 kb on + strand, within EX31_RS14640 at 20.758 kb on - strand, within EX31_RS14640 at 20.758 kb on - strand, within EX31_RS14640 at 20.793 kb on + strand, within EX31_RS14640 at 20.793 kb on + strand, within EX31_RS14640 at 20.793 kb on + strand, within EX31_RS14640 at 20.793 kb on + strand, within EX31_RS14640 at 20.794 kb on - strand, within EX31_RS14640 at 20.794 kb on - strand, within EX31_RS14640 at 20.815 kb on - strand, within EX31_RS14640 at 20.971 kb on + strand at 20.971 kb on + strand at 20.972 kb on - strand at 21.018 kb on + strand at 21.018 kb on + strand at 21.018 kb on + strand at 21.018 kb on + strand at 21.019 kb on - strand at 21.019 kb on - strand at 21.037 kb on + strand at 21.037 kb on + strand at 21.037 kb on + strand at 21.038 kb on - strand at 21.038 kb on - strand at 21.047 kb on + strand at 21.047 kb on + strand at 21.047 kb on + strand at 21.047 kb on + strand at 21.047 kb on + strand at 21.075 kb on + strand at 21.075 kb on + strand at 21.075 kb on + strand at 21.076 kb on - strand at 21.094 kb on - strand at 21.113 kb on + strand at 21.113 kb on + strand at 21.113 kb on + strand at 21.113 kb on + strand at 21.114 kb on - strand at 21.114 kb on - strand at 21.114 kb on - strand at 21.114 kb on - strand at 21.114 kb on - strand at 21.114 kb on - strand at 21.120 kb on + strand at 21.120 kb on + strand at 21.120 kb on + strand at 21.120 kb on + strand at 21.120 kb on + strand at 21.120 kb on + strand at 21.120 kb on + strand at 21.120 kb on + strand at 21.121 kb on - strand at 21.121 kb on - strand at 21.121 kb on - strand at 21.139 kb on + strand at 21.139 kb on + strand at 21.140 kb on - strand at 21.140 kb on - strand at 21.140 kb on - strand at 21.140 kb on - strand at 21.145 kb on + strand at 21.145 kb on + strand at 21.146 kb on - strand at 21.170 kb on - strand at 21.233 kb on + strand at 21.234 kb on - strand at 21.234 kb on - strand at 21.234 kb on - strand at 21.234 kb on - strand at 21.239 kb on + strand at 21.240 kb on - strand at 21.416 kb on + strand at 21.416 kb on + strand at 21.416 kb on + strand at 21.416 kb on + strand at 21.416 kb on + strand at 21.416 kb on + strand at 21.417 kb on - strand at 21.441 kb on + strand at 21.441 kb on + strand at 21.442 kb on - strand at 21.442 kb on - strand at 21.442 kb on - strand at 21.442 kb on - strand at 21.456 kb on + strand at 21.456 kb on + strand at 21.456 kb on + strand at 21.456 kb on + strand at 21.456 kb on + strand at 21.456 kb on + strand at 21.457 kb on - strand at 21.457 kb on - strand at 21.457 kb on - strand at 21.457 kb on - strand at 21.457 kb on - strand at 21.457 kb on - strand at 21.568 kb on + strand, within EX31_RS14645 at 21.568 kb on + strand, within EX31_RS14645 at 21.568 kb on + strand, within EX31_RS14645 at 21.568 kb on + strand, within EX31_RS14645 at 21.568 kb on + strand, within EX31_RS14645 at 21.568 kb on + strand, within EX31_RS14645 at 21.568 kb on + strand, within EX31_RS14645 at 21.568 kb on + strand, within EX31_RS14645 at 21.568 kb on + strand, within EX31_RS14645 at 21.569 kb on - strand, within EX31_RS14645 at 21.569 kb on - strand, within EX31_RS14645 at 21.569 kb on - strand, within EX31_RS14645 at 21.569 kb on - strand, within EX31_RS14645 at 21.569 kb on - strand, within EX31_RS14645 at 21.569 kb on - strand, within EX31_RS14645 at 21.569 kb on - strand, within EX31_RS14645 at 21.569 kb on - strand, within EX31_RS14645 at 21.569 kb on - strand, within EX31_RS14645 at 21.569 kb on - strand, within EX31_RS14645 at 21.569 kb on - strand, within EX31_RS14645 at 21.569 kb on - strand, within EX31_RS14645 at 21.596 kb on + strand, within EX31_RS14645 at 21.616 kb on + strand, within EX31_RS14645 at 21.616 kb on + strand, within EX31_RS14645 at 21.647 kb on + strand, within EX31_RS14645 at 21.647 kb on + strand, within EX31_RS14645 at 21.648 kb on - strand, within EX31_RS14645 at 21.648 kb on - strand, within EX31_RS14645 at 21.648 kb on - strand, within EX31_RS14645 at 21.735 kb on + strand, within EX31_RS14645 at 21.735 kb on + strand, within EX31_RS14645 at 21.735 kb on + strand, within EX31_RS14645 at 21.736 kb on - strand, within EX31_RS14645 at 21.736 kb on - strand, within EX31_RS14645 at 21.736 kb on - strand, within EX31_RS14645 at 21.814 kb on + strand, within EX31_RS14645 at 21.814 kb on + strand, within EX31_RS14645 at 21.814 kb on + strand, within EX31_RS14645 at 21.815 kb on - strand, within EX31_RS14645 at 21.815 kb on - strand, within EX31_RS14645 at 21.818 kb on - strand, within EX31_RS14645 at 21.892 kb on + strand, within EX31_RS14645 at 21.894 kb on + strand, within EX31_RS14645 at 21.894 kb on + strand, within EX31_RS14645 at 21.894 kb on + strand, within EX31_RS14645 at 21.894 kb on + strand, within EX31_RS14645 at 21.895 kb on - strand, within EX31_RS14645 at 21.895 kb on - strand, within EX31_RS14645 at 21.938 kb on - strand, within EX31_RS14645 at 21.940 kb on + strand, within EX31_RS14645 at 21.940 kb on + strand, within EX31_RS14645 at 21.940 kb on + strand, within EX31_RS14645 at 21.940 kb on + strand, within EX31_RS14645 at 21.940 kb on + strand, within EX31_RS14645 at 21.940 kb on + strand, within EX31_RS14645 at 21.941 kb on - strand, within EX31_RS14645 at 21.941 kb on - strand, within EX31_RS14645 at 21.941 kb on - strand, within EX31_RS14645 at 21.941 kb on - strand, within EX31_RS14645 at 21.941 kb on - strand, within EX31_RS14645 at 21.941 kb on - strand, within EX31_RS14645 at 21.941 kb on - strand, within EX31_RS14645 at 21.993 kb on + strand, within EX31_RS14645 at 21.993 kb on + strand, within EX31_RS14645 at 21.993 kb on + strand, within EX31_RS14645 at 21.993 kb on + strand, within EX31_RS14645 at 21.994 kb on - strand, within EX31_RS14645 at 21.994 kb on - strand, within EX31_RS14645 at 21.994 kb on - strand, within EX31_RS14645 at 21.994 kb on - strand, within EX31_RS14645 at 21.994 kb on - strand, within EX31_RS14645 at 21.994 kb on - strand, within EX31_RS14645 at 21.994 kb on - strand, within EX31_RS14645 at 21.997 kb on - strand, within EX31_RS14645
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 19,263 - -0.0 19,263 - -0.5 19,306 + +0.7 19,354 - +0.5 19,359 + -1.1 19,360 - +0.6 19,360 - +0.2 19,441 + -0.9 19,442 - +0.1 19,442 - +1.5 19,524 + +0.3 19,524 + +0.3 19,525 - +0.1 19,525 - -0.6 19,853 + EX31_RS14635 0.55 -1.1 19,854 - EX31_RS14635 0.55 +0.2 19,957 - EX31_RS14635 0.74 +0.5 19,957 - EX31_RS14635 0.74 +1.0 19,957 - EX31_RS14635 0.74 +1.0 19,957 - EX31_RS14635 0.74 -0.6 20,058 + +0.1 20,058 + +1.1 20,059 - -0.8 20,059 - -0.2 20,059 - -0.6 20,059 - +0.3 20,066 + +0.1 20,137 + -0.2 20,138 - +0.6 20,151 + -0.1 20,151 + +0.5 20,151 + +0.4 20,151 + +0.3 20,152 - +0.1 20,154 + +0.1 20,154 + +1.1 20,154 + +0.6 20,155 - -0.1 20,178 + +0.5 20,243 + -0.5 20,243 + +0.2 20,249 + -0.0 20,250 - -0.5 20,250 - +0.2 20,250 - -0.2 20,251 + -0.7 20,258 + +0.0 20,258 + +0.1 20,258 + -0.7 20,258 + -1.1 20,259 - +1.2 20,259 - +0.2 20,259 - +0.2 20,262 + +0.6 20,262 + +0.4 20,262 + -0.1 20,262 + +0.2 20,262 + +0.6 20,263 - +0.5 20,263 - -1.1 20,263 - +0.0 20,281 + -0.1 20,282 - +1.0 20,282 - +0.2 20,435 + EX31_RS14640 0.23 +0.1 20,436 - EX31_RS14640 0.23 -0.3 20,504 + EX31_RS14640 0.32 -0.6 20,504 + EX31_RS14640 0.32 -0.2 20,504 + EX31_RS14640 0.32 +1.3 20,505 - EX31_RS14640 0.33 -1.2 20,505 - EX31_RS14640 0.33 -0.5 20,505 - EX31_RS14640 0.33 +0.5 20,505 - EX31_RS14640 0.33 +0.8 20,505 - EX31_RS14640 0.33 +0.4 20,505 - EX31_RS14640 0.33 +1.2 20,514 - EX31_RS14640 0.34 -0.2 20,531 + EX31_RS14640 0.36 +0.4 20,757 + EX31_RS14640 0.66 -0.0 20,757 + EX31_RS14640 0.66 +0.4 20,757 + EX31_RS14640 0.66 +0.1 20,757 + EX31_RS14640 0.66 -0.4 20,758 - EX31_RS14640 0.66 -1.2 20,758 - EX31_RS14640 0.66 +0.5 20,793 + EX31_RS14640 0.70 +1.1 20,793 + EX31_RS14640 0.70 +0.4 20,793 + EX31_RS14640 0.70 -0.6 20,793 + EX31_RS14640 0.70 -0.3 20,794 - EX31_RS14640 0.70 -0.3 20,794 - EX31_RS14640 0.70 -0.4 20,815 - EX31_RS14640 0.73 +0.4 20,971 + +0.5 20,971 + -0.1 20,972 - +0.6 21,018 + +0.3 21,018 + -0.8 21,018 + +0.5 21,018 + +0.5 21,019 - -0.6 21,019 - +0.2 21,037 + -0.5 21,037 + -0.5 21,037 + +0.0 21,038 - +0.3 21,038 - +0.1 21,047 + +1.0 21,047 + +0.1 21,047 + +0.8 21,047 + -0.7 21,047 + +0.4 21,075 + +1.4 21,075 + -1.9 21,075 + +0.3 21,076 - +0.4 21,094 - +0.5 21,113 + -1.1 21,113 + -0.0 21,113 + +0.4 21,113 + -0.0 21,114 - -0.5 21,114 - -0.3 21,114 - -0.0 21,114 - -1.0 21,114 - +0.3 21,114 - -0.5 21,120 + -0.2 21,120 + +0.0 21,120 + +0.3 21,120 + -0.9 21,120 + -0.2 21,120 + +0.2 21,120 + -0.5 21,120 + +0.5 21,121 - +1.6 21,121 - -0.4 21,121 - -1.1 21,139 + +1.3 21,139 + -1.1 21,140 - -0.4 21,140 - +0.2 21,140 - -0.4 21,140 - +0.9 21,145 + +0.1 21,145 + +0.2 21,146 - +0.6 21,170 - +0.0 21,233 + -0.4 21,234 - +0.3 21,234 - +0.9 21,234 - +0.1 21,234 - -1.0 21,239 + -0.5 21,240 - +0.3 21,416 + -0.1 21,416 + +1.9 21,416 + +1.0 21,416 + -0.2 21,416 + +0.5 21,416 + -0.1 21,417 - -0.3 21,441 + -1.1 21,441 + +0.2 21,442 - -0.1 21,442 - +0.3 21,442 - -1.6 21,442 - +0.6 21,456 + -0.4 21,456 + +0.2 21,456 + +0.1 21,456 + +0.9 21,456 + -0.1 21,456 + -1.1 21,457 - +0.6 21,457 - +1.4 21,457 - +0.1 21,457 - +1.2 21,457 - -0.7 21,457 - +0.4 21,568 + EX31_RS14645 0.14 -0.5 21,568 + EX31_RS14645 0.14 +0.8 21,568 + EX31_RS14645 0.14 -0.7 21,568 + EX31_RS14645 0.14 -0.8 21,568 + EX31_RS14645 0.14 +0.5 21,568 + EX31_RS14645 0.14 -0.8 21,568 + EX31_RS14645 0.14 -0.6 21,568 + EX31_RS14645 0.14 +0.2 21,568 + EX31_RS14645 0.14 +1.3 21,569 - EX31_RS14645 0.14 -0.6 21,569 - EX31_RS14645 0.14 +0.9 21,569 - EX31_RS14645 0.14 +0.5 21,569 - EX31_RS14645 0.14 +1.2 21,569 - EX31_RS14645 0.14 +0.2 21,569 - EX31_RS14645 0.14 +0.3 21,569 - EX31_RS14645 0.14 +0.1 21,569 - EX31_RS14645 0.14 +0.4 21,569 - EX31_RS14645 0.14 -0.3 21,569 - EX31_RS14645 0.14 +1.2 21,569 - EX31_RS14645 0.14 -0.0 21,569 - EX31_RS14645 0.14 -0.7 21,596 + EX31_RS14645 0.16 +0.4 21,616 + EX31_RS14645 0.17 +0.1 21,616 + EX31_RS14645 0.17 +0.8 21,647 + EX31_RS14645 0.18 -0.5 21,647 + EX31_RS14645 0.18 -1.1 21,648 - EX31_RS14645 0.18 +0.3 21,648 - EX31_RS14645 0.18 +0.1 21,648 - EX31_RS14645 0.18 -0.2 21,735 + EX31_RS14645 0.22 +0.5 21,735 + EX31_RS14645 0.22 -0.8 21,735 + EX31_RS14645 0.22 -0.2 21,736 - EX31_RS14645 0.22 +0.4 21,736 - EX31_RS14645 0.22 -0.3 21,736 - EX31_RS14645 0.22 +0.1 21,814 + EX31_RS14645 0.25 +0.3 21,814 + EX31_RS14645 0.25 +0.3 21,814 + EX31_RS14645 0.25 +0.5 21,815 - EX31_RS14645 0.25 +0.9 21,815 - EX31_RS14645 0.25 +0.5 21,818 - EX31_RS14645 0.25 +0.4 21,892 + EX31_RS14645 0.29 -0.4 21,894 + EX31_RS14645 0.29 -0.5 21,894 + EX31_RS14645 0.29 -0.7 21,894 + EX31_RS14645 0.29 +0.5 21,894 + EX31_RS14645 0.29 -0.2 21,895 - EX31_RS14645 0.29 -0.1 21,895 - EX31_RS14645 0.29 -0.3 21,938 - EX31_RS14645 0.31 +0.6 21,940 + EX31_RS14645 0.31 +0.1 21,940 + EX31_RS14645 0.31 +0.5 21,940 + EX31_RS14645 0.31 +0.6 21,940 + EX31_RS14645 0.31 +1.2 21,940 + EX31_RS14645 0.31 -1.4 21,940 + EX31_RS14645 0.31 +0.2 21,941 - EX31_RS14645 0.31 -0.6 21,941 - EX31_RS14645 0.31 -0.4 21,941 - EX31_RS14645 0.31 +0.0 21,941 - EX31_RS14645 0.31 +0.0 21,941 - EX31_RS14645 0.31 -0.1 21,941 - EX31_RS14645 0.31 -0.5 21,941 - EX31_RS14645 0.31 -1.1 21,993 + EX31_RS14645 0.33 -0.1 21,993 + EX31_RS14645 0.33 -0.9 21,993 + EX31_RS14645 0.33 -1.7 21,993 + EX31_RS14645 0.33 +0.8 21,994 - EX31_RS14645 0.33 +0.5 21,994 - EX31_RS14645 0.33 -0.1 21,994 - EX31_RS14645 0.33 +0.6 21,994 - EX31_RS14645 0.33 -0.7 21,994 - EX31_RS14645 0.33 -1.7 21,994 - EX31_RS14645 0.33 -0.6 21,994 - EX31_RS14645 0.33 -1.0 21,997 - EX31_RS14645 0.33 -1.2
Or see this region's nucleotide sequence