Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS14375 and EX31_RS14380 are separated by 2 nucleotides EX31_RS14380 and EX31_RS14385 are separated by 67 nucleotides EX31_RS14385 and EX31_RS14390 are separated by 9 nucleotides
EX31_RS14375: EX31_RS14375 - glycosyltransferase, at 56,118 to 57,341
_RS14375
EX31_RS14380: EX31_RS14380 - hypothetical protein, at 57,344 to 58,273
_RS14380
EX31_RS14385: EX31_RS14385 - L-threonine 3-dehydrogenase, at 58,341 to 59,363
_RS14385
EX31_RS14390: EX31_RS14390 - glycine C-acetyltransferase, at 59,373 to 60,569
_RS14390
Position (kb) 58
59
60 Strain fitness (log2 ratio) -2
-1
0
1
2 at 57.343 kb on + strand at 57.343 kb on + strand at 57.343 kb on + strand at 57.344 kb on - strand at 57.344 kb on - strand at 57.391 kb on + strand at 57.476 kb on + strand, within EX31_RS14380 at 57.476 kb on + strand, within EX31_RS14380 at 57.476 kb on + strand, within EX31_RS14380 at 57.476 kb on + strand, within EX31_RS14380 at 57.476 kb on + strand, within EX31_RS14380 at 57.477 kb on - strand, within EX31_RS14380 at 57.477 kb on - strand, within EX31_RS14380 at 57.477 kb on - strand, within EX31_RS14380 at 57.477 kb on - strand, within EX31_RS14380 at 57.477 kb on - strand, within EX31_RS14380 at 57.477 kb on - strand, within EX31_RS14380 at 57.532 kb on - strand, within EX31_RS14380 at 57.532 kb on - strand, within EX31_RS14380 at 57.635 kb on + strand, within EX31_RS14380 at 57.642 kb on - strand, within EX31_RS14380 at 57.643 kb on + strand, within EX31_RS14380 at 57.643 kb on + strand, within EX31_RS14380 at 57.644 kb on - strand, within EX31_RS14380 at 57.644 kb on - strand, within EX31_RS14380 at 57.707 kb on - strand, within EX31_RS14380 at 57.773 kb on - strand, within EX31_RS14380 at 57.777 kb on - strand, within EX31_RS14380 at 57.845 kb on + strand, within EX31_RS14380 at 57.845 kb on + strand, within EX31_RS14380 at 57.845 kb on + strand, within EX31_RS14380 at 57.846 kb on - strand, within EX31_RS14380 at 57.846 kb on - strand, within EX31_RS14380 at 57.846 kb on - strand, within EX31_RS14380 at 57.869 kb on + strand, within EX31_RS14380 at 57.869 kb on + strand, within EX31_RS14380 at 57.869 kb on + strand, within EX31_RS14380 at 57.869 kb on + strand, within EX31_RS14380 at 57.870 kb on - strand, within EX31_RS14380 at 57.870 kb on - strand, within EX31_RS14380 at 57.870 kb on - strand, within EX31_RS14380 at 57.870 kb on - strand, within EX31_RS14380 at 57.871 kb on + strand, within EX31_RS14380 at 57.871 kb on + strand, within EX31_RS14380 at 57.871 kb on + strand, within EX31_RS14380 at 57.871 kb on + strand, within EX31_RS14380 at 57.872 kb on - strand, within EX31_RS14380 at 57.872 kb on - strand, within EX31_RS14380 at 57.872 kb on - strand, within EX31_RS14380 at 57.872 kb on - strand, within EX31_RS14380 at 57.913 kb on + strand, within EX31_RS14380 at 57.913 kb on + strand, within EX31_RS14380 at 57.913 kb on + strand, within EX31_RS14380 at 57.946 kb on + strand, within EX31_RS14380 at 57.946 kb on + strand, within EX31_RS14380 at 57.961 kb on + strand, within EX31_RS14380 at 57.961 kb on + strand, within EX31_RS14380 at 57.961 kb on + strand, within EX31_RS14380 at 57.961 kb on + strand, within EX31_RS14380 at 57.961 kb on + strand, within EX31_RS14380 at 57.962 kb on - strand, within EX31_RS14380 at 57.962 kb on - strand, within EX31_RS14380 at 57.962 kb on - strand, within EX31_RS14380 at 57.962 kb on - strand, within EX31_RS14380 at 57.962 kb on - strand, within EX31_RS14380 at 57.962 kb on - strand, within EX31_RS14380 at 57.962 kb on - strand, within EX31_RS14380 at 58.114 kb on + strand, within EX31_RS14380 at 58.166 kb on + strand, within EX31_RS14380 at 58.167 kb on - strand, within EX31_RS14380 at 58.194 kb on + strand at 58.195 kb on - strand at 58.212 kb on - strand at 58.230 kb on - strand at 58.271 kb on + strand at 58.349 kb on + strand at 58.349 kb on + strand at 58.349 kb on + strand at 58.349 kb on + strand at 58.349 kb on + strand at 58.349 kb on + strand at 58.349 kb on + strand at 58.349 kb on + strand at 58.349 kb on + strand at 58.349 kb on + strand at 58.349 kb on + strand at 58.349 kb on + strand at 58.349 kb on + strand at 58.350 kb on - strand at 58.350 kb on - strand at 58.350 kb on - strand at 58.350 kb on - strand at 58.350 kb on - strand at 58.350 kb on - strand at 58.350 kb on - strand at 58.350 kb on - strand at 58.350 kb on - strand at 58.350 kb on - strand at 58.350 kb on - strand at 58.350 kb on - strand at 58.350 kb on - strand at 58.354 kb on - strand at 58.354 kb on - strand at 58.439 kb on + strand at 58.439 kb on + strand at 58.439 kb on + strand at 58.439 kb on + strand at 58.439 kb on + strand at 58.439 kb on + strand at 58.439 kb on + strand at 58.439 kb on + strand at 58.440 kb on - strand at 58.440 kb on - strand at 58.440 kb on - strand at 58.440 kb on - strand at 58.440 kb on - strand at 58.440 kb on - strand at 58.440 kb on - strand at 58.477 kb on + strand, within EX31_RS14385 at 58.504 kb on + strand, within EX31_RS14385 at 58.768 kb on + strand, within EX31_RS14385 at 58.778 kb on + strand, within EX31_RS14385 at 58.949 kb on + strand, within EX31_RS14385 at 58.949 kb on + strand, within EX31_RS14385 at 58.950 kb on - strand, within EX31_RS14385 at 58.983 kb on + strand, within EX31_RS14385 at 58.987 kb on - strand, within EX31_RS14385 at 58.993 kb on + strand, within EX31_RS14385 at 58.993 kb on + strand, within EX31_RS14385 at 58.993 kb on + strand, within EX31_RS14385 at 58.994 kb on - strand, within EX31_RS14385 at 59.054 kb on + strand, within EX31_RS14385 at 59.055 kb on - strand, within EX31_RS14385 at 59.072 kb on + strand, within EX31_RS14385 at 59.072 kb on + strand, within EX31_RS14385 at 59.090 kb on + strand, within EX31_RS14385 at 59.090 kb on + strand, within EX31_RS14385 at 59.090 kb on + strand, within EX31_RS14385 at 59.091 kb on - strand, within EX31_RS14385 at 59.091 kb on - strand, within EX31_RS14385 at 59.091 kb on - strand, within EX31_RS14385 at 59.091 kb on - strand, within EX31_RS14385 at 59.091 kb on - strand, within EX31_RS14385 at 59.091 kb on - strand, within EX31_RS14385 at 59.093 kb on + strand, within EX31_RS14385 at 59.093 kb on + strand, within EX31_RS14385 at 59.093 kb on + strand, within EX31_RS14385 at 59.093 kb on + strand, within EX31_RS14385 at 59.093 kb on + strand, within EX31_RS14385 at 59.093 kb on + strand, within EX31_RS14385 at 59.093 kb on + strand, within EX31_RS14385 at 59.093 kb on + strand, within EX31_RS14385 at 59.094 kb on - strand, within EX31_RS14385 at 59.094 kb on - strand, within EX31_RS14385 at 59.094 kb on - strand, within EX31_RS14385 at 59.094 kb on - strand, within EX31_RS14385 at 59.136 kb on - strand, within EX31_RS14385 at 59.170 kb on + strand, within EX31_RS14385 at 59.170 kb on + strand, within EX31_RS14385 at 59.170 kb on + strand, within EX31_RS14385 at 59.170 kb on + strand, within EX31_RS14385 at 59.171 kb on - strand, within EX31_RS14385 at 59.171 kb on - strand, within EX31_RS14385 at 59.171 kb on - strand, within EX31_RS14385 at 59.171 kb on - strand, within EX31_RS14385 at 59.171 kb on - strand, within EX31_RS14385 at 59.171 kb on - strand, within EX31_RS14385 at 59.171 kb on - strand, within EX31_RS14385 at 59.171 kb on - strand, within EX31_RS14385 at 59.230 kb on + strand, within EX31_RS14385 at 59.230 kb on + strand, within EX31_RS14385 at 59.230 kb on + strand, within EX31_RS14385 at 59.230 kb on + strand, within EX31_RS14385 at 59.230 kb on + strand, within EX31_RS14385 at 59.231 kb on - strand, within EX31_RS14385 at 59.231 kb on - strand, within EX31_RS14385 at 59.231 kb on - strand, within EX31_RS14385 at 59.231 kb on - strand, within EX31_RS14385 at 59.295 kb on + strand at 59.296 kb on - strand at 59.352 kb on + strand at 59.402 kb on + strand at 59.403 kb on - strand at 59.501 kb on - strand, within EX31_RS14390 at 59.518 kb on + strand, within EX31_RS14390 at 59.518 kb on + strand, within EX31_RS14390 at 59.747 kb on + strand, within EX31_RS14390 at 59.748 kb on - strand, within EX31_RS14390 at 59.759 kb on - strand, within EX31_RS14390 at 59.759 kb on - strand, within EX31_RS14390 at 59.810 kb on + strand, within EX31_RS14390 at 59.811 kb on - strand, within EX31_RS14390 at 59.811 kb on - strand, within EX31_RS14390 at 59.811 kb on - strand, within EX31_RS14390 at 59.887 kb on + strand, within EX31_RS14390 at 60.001 kb on - strand, within EX31_RS14390 at 60.021 kb on + strand, within EX31_RS14390 at 60.021 kb on + strand, within EX31_RS14390 at 60.021 kb on + strand, within EX31_RS14390 at 60.022 kb on - strand, within EX31_RS14390 at 60.045 kb on - strand, within EX31_RS14390 at 60.045 kb on - strand, within EX31_RS14390 at 60.098 kb on + strand, within EX31_RS14390 at 60.098 kb on + strand, within EX31_RS14390 at 60.098 kb on + strand, within EX31_RS14390 at 60.098 kb on + strand, within EX31_RS14390 at 60.098 kb on + strand, within EX31_RS14390 at 60.098 kb on + strand, within EX31_RS14390 at 60.099 kb on - strand, within EX31_RS14390 at 60.099 kb on - strand, within EX31_RS14390 at 60.099 kb on - strand, within EX31_RS14390 at 60.099 kb on - strand, within EX31_RS14390 at 60.099 kb on - strand, within EX31_RS14390 at 60.099 kb on - strand, within EX31_RS14390 at 60.099 kb on - strand, within EX31_RS14390 at 60.135 kb on + strand, within EX31_RS14390 at 60.135 kb on + strand, within EX31_RS14390 at 60.136 kb on - strand, within EX31_RS14390 at 60.136 kb on - strand, within EX31_RS14390 at 60.153 kb on + strand, within EX31_RS14390 at 60.216 kb on + strand, within EX31_RS14390 at 60.216 kb on + strand, within EX31_RS14390 at 60.217 kb on - strand, within EX31_RS14390 at 60.247 kb on + strand, within EX31_RS14390 at 60.248 kb on - strand, within EX31_RS14390 at 60.248 kb on - strand, within EX31_RS14390 at 60.308 kb on + strand, within EX31_RS14390 at 60.308 kb on + strand, within EX31_RS14390 at 60.308 kb on + strand, within EX31_RS14390 at 60.308 kb on + strand, within EX31_RS14390 at 60.308 kb on + strand, within EX31_RS14390 at 60.308 kb on + strand, within EX31_RS14390 at 60.308 kb on + strand, within EX31_RS14390 at 60.308 kb on + strand, within EX31_RS14390 at 60.308 kb on + strand, within EX31_RS14390 at 60.309 kb on - strand, within EX31_RS14390 at 60.309 kb on - strand, within EX31_RS14390 at 60.326 kb on + strand, within EX31_RS14390
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 57,343 + -0.4 57,343 + +0.1 57,343 + +1.0 57,344 - +1.0 57,344 - +2.1 57,391 + +0.4 57,476 + EX31_RS14380 0.14 +0.6 57,476 + EX31_RS14380 0.14 -0.6 57,476 + EX31_RS14380 0.14 +0.2 57,476 + EX31_RS14380 0.14 +0.1 57,476 + EX31_RS14380 0.14 +1.0 57,477 - EX31_RS14380 0.14 -0.4 57,477 - EX31_RS14380 0.14 +0.9 57,477 - EX31_RS14380 0.14 -0.1 57,477 - EX31_RS14380 0.14 +0.8 57,477 - EX31_RS14380 0.14 +0.4 57,477 - EX31_RS14380 0.14 -0.5 57,532 - EX31_RS14380 0.20 +0.5 57,532 - EX31_RS14380 0.20 +0.4 57,635 + EX31_RS14380 0.31 +0.2 57,642 - EX31_RS14380 0.32 +0.2 57,643 + EX31_RS14380 0.32 +0.4 57,643 + EX31_RS14380 0.32 +0.4 57,644 - EX31_RS14380 0.32 -0.9 57,644 - EX31_RS14380 0.32 +0.2 57,707 - EX31_RS14380 0.39 +0.2 57,773 - EX31_RS14380 0.46 -0.2 57,777 - EX31_RS14380 0.47 +0.8 57,845 + EX31_RS14380 0.54 +0.1 57,845 + EX31_RS14380 0.54 +0.0 57,845 + EX31_RS14380 0.54 +1.3 57,846 - EX31_RS14380 0.54 +0.5 57,846 - EX31_RS14380 0.54 -1.0 57,846 - EX31_RS14380 0.54 +0.9 57,869 + EX31_RS14380 0.56 -0.3 57,869 + EX31_RS14380 0.56 -0.7 57,869 + EX31_RS14380 0.56 -0.1 57,869 + EX31_RS14380 0.56 -0.1 57,870 - EX31_RS14380 0.57 -0.1 57,870 - EX31_RS14380 0.57 -0.6 57,870 - EX31_RS14380 0.57 +0.0 57,870 - EX31_RS14380 0.57 +0.1 57,871 + EX31_RS14380 0.57 -0.1 57,871 + EX31_RS14380 0.57 +0.8 57,871 + EX31_RS14380 0.57 +0.4 57,871 + EX31_RS14380 0.57 -0.4 57,872 - EX31_RS14380 0.57 -0.4 57,872 - EX31_RS14380 0.57 +0.1 57,872 - EX31_RS14380 0.57 +0.1 57,872 - EX31_RS14380 0.57 -0.4 57,913 + EX31_RS14380 0.61 -0.4 57,913 + EX31_RS14380 0.61 +0.7 57,913 + EX31_RS14380 0.61 -0.6 57,946 + EX31_RS14380 0.65 -0.4 57,946 + EX31_RS14380 0.65 +0.4 57,961 + EX31_RS14380 0.66 -2.3 57,961 + EX31_RS14380 0.66 -0.5 57,961 + EX31_RS14380 0.66 -0.7 57,961 + EX31_RS14380 0.66 -1.3 57,961 + EX31_RS14380 0.66 -1.8 57,962 - EX31_RS14380 0.66 -0.2 57,962 - EX31_RS14380 0.66 -0.3 57,962 - EX31_RS14380 0.66 +0.3 57,962 - EX31_RS14380 0.66 -0.5 57,962 - EX31_RS14380 0.66 -0.1 57,962 - EX31_RS14380 0.66 +0.4 57,962 - EX31_RS14380 0.66 +0.3 58,114 + EX31_RS14380 0.83 +0.6 58,166 + EX31_RS14380 0.88 -0.7 58,167 - EX31_RS14380 0.88 -2.4 58,194 + -0.3 58,195 - -0.4 58,212 - -0.6 58,230 - +0.3 58,271 + -0.2 58,349 + +0.4 58,349 + +0.4 58,349 + +1.1 58,349 + -0.5 58,349 + -0.6 58,349 + -1.9 58,349 + +0.3 58,349 + -1.8 58,349 + +0.2 58,349 + -0.2 58,349 + -0.1 58,349 + +0.1 58,349 + -0.2 58,350 - +0.7 58,350 - -0.9 58,350 - -0.6 58,350 - -0.5 58,350 - +0.2 58,350 - +0.6 58,350 - +1.1 58,350 - -0.1 58,350 - +0.7 58,350 - +0.0 58,350 - +0.0 58,350 - +0.3 58,350 - +0.8 58,354 - +1.3 58,354 - +0.1 58,439 + -0.2 58,439 + -0.2 58,439 + +0.2 58,439 + -0.4 58,439 + +0.7 58,439 + +0.1 58,439 + +0.5 58,439 + +0.1 58,440 - -0.7 58,440 - +0.0 58,440 - +0.5 58,440 - +0.1 58,440 - +0.4 58,440 - +1.0 58,440 - -0.3 58,477 + EX31_RS14385 0.13 -1.7 58,504 + EX31_RS14385 0.16 -0.2 58,768 + EX31_RS14385 0.42 +0.2 58,778 + EX31_RS14385 0.43 -0.7 58,949 + EX31_RS14385 0.59 +0.2 58,949 + EX31_RS14385 0.59 +0.4 58,950 - EX31_RS14385 0.60 +0.6 58,983 + EX31_RS14385 0.63 +0.1 58,987 - EX31_RS14385 0.63 +0.1 58,993 + EX31_RS14385 0.64 -0.2 58,993 + EX31_RS14385 0.64 -1.5 58,993 + EX31_RS14385 0.64 +0.8 58,994 - EX31_RS14385 0.64 -1.1 59,054 + EX31_RS14385 0.70 +1.7 59,055 - EX31_RS14385 0.70 +0.6 59,072 + EX31_RS14385 0.71 +0.7 59,072 + EX31_RS14385 0.71 +0.1 59,090 + EX31_RS14385 0.73 +0.4 59,090 + EX31_RS14385 0.73 +1.4 59,090 + EX31_RS14385 0.73 +0.6 59,091 - EX31_RS14385 0.73 -0.4 59,091 - EX31_RS14385 0.73 -2.7 59,091 - EX31_RS14385 0.73 +0.6 59,091 - EX31_RS14385 0.73 +0.6 59,091 - EX31_RS14385 0.73 +0.2 59,091 - EX31_RS14385 0.73 +0.5 59,093 + EX31_RS14385 0.74 -0.4 59,093 + EX31_RS14385 0.74 +0.8 59,093 + EX31_RS14385 0.74 +0.5 59,093 + EX31_RS14385 0.74 -0.6 59,093 + EX31_RS14385 0.74 -0.5 59,093 + EX31_RS14385 0.74 +0.4 59,093 + EX31_RS14385 0.74 -0.7 59,093 + EX31_RS14385 0.74 -0.6 59,094 - EX31_RS14385 0.74 -0.4 59,094 - EX31_RS14385 0.74 +0.3 59,094 - EX31_RS14385 0.74 +0.0 59,094 - EX31_RS14385 0.74 -0.8 59,136 - EX31_RS14385 0.78 +1.0 59,170 + EX31_RS14385 0.81 +0.6 59,170 + EX31_RS14385 0.81 +1.2 59,170 + EX31_RS14385 0.81 -1.1 59,170 + EX31_RS14385 0.81 -0.0 59,171 - EX31_RS14385 0.81 -0.3 59,171 - EX31_RS14385 0.81 +0.6 59,171 - EX31_RS14385 0.81 -0.5 59,171 - EX31_RS14385 0.81 +0.3 59,171 - EX31_RS14385 0.81 -0.2 59,171 - EX31_RS14385 0.81 -0.1 59,171 - EX31_RS14385 0.81 +1.1 59,171 - EX31_RS14385 0.81 +1.4 59,230 + EX31_RS14385 0.87 -0.6 59,230 + EX31_RS14385 0.87 -0.3 59,230 + EX31_RS14385 0.87 +0.4 59,230 + EX31_RS14385 0.87 +1.4 59,230 + EX31_RS14385 0.87 +1.0 59,231 - EX31_RS14385 0.87 +0.4 59,231 - EX31_RS14385 0.87 +0.7 59,231 - EX31_RS14385 0.87 +0.2 59,231 - EX31_RS14385 0.87 +0.4 59,295 + +0.4 59,296 - +0.3 59,352 + -0.2 59,402 + +0.3 59,403 - +0.2 59,501 - EX31_RS14390 0.11 +0.3 59,518 + EX31_RS14390 0.12 -0.9 59,518 + EX31_RS14390 0.12 -0.1 59,747 + EX31_RS14390 0.31 +1.0 59,748 - EX31_RS14390 0.31 +0.3 59,759 - EX31_RS14390 0.32 +0.0 59,759 - EX31_RS14390 0.32 +0.0 59,810 + EX31_RS14390 0.37 +0.3 59,811 - EX31_RS14390 0.37 +0.7 59,811 - EX31_RS14390 0.37 +0.6 59,811 - EX31_RS14390 0.37 -0.5 59,887 + EX31_RS14390 0.43 -0.3 60,001 - EX31_RS14390 0.52 -0.4 60,021 + EX31_RS14390 0.54 +0.2 60,021 + EX31_RS14390 0.54 +0.1 60,021 + EX31_RS14390 0.54 +0.4 60,022 - EX31_RS14390 0.54 -0.6 60,045 - EX31_RS14390 0.56 -1.0 60,045 - EX31_RS14390 0.56 +0.4 60,098 + EX31_RS14390 0.61 -0.2 60,098 + EX31_RS14390 0.61 +0.3 60,098 + EX31_RS14390 0.61 +0.6 60,098 + EX31_RS14390 0.61 -0.5 60,098 + EX31_RS14390 0.61 +0.2 60,098 + EX31_RS14390 0.61 +0.5 60,099 - EX31_RS14390 0.61 -0.2 60,099 - EX31_RS14390 0.61 -0.0 60,099 - EX31_RS14390 0.61 +1.5 60,099 - EX31_RS14390 0.61 +0.0 60,099 - EX31_RS14390 0.61 -0.5 60,099 - EX31_RS14390 0.61 -0.7 60,099 - EX31_RS14390 0.61 +0.2 60,135 + EX31_RS14390 0.64 -0.4 60,135 + EX31_RS14390 0.64 -1.0 60,136 - EX31_RS14390 0.64 +0.2 60,136 - EX31_RS14390 0.64 +1.1 60,153 + EX31_RS14390 0.65 -0.3 60,216 + EX31_RS14390 0.70 -0.6 60,216 + EX31_RS14390 0.70 -0.6 60,217 - EX31_RS14390 0.71 +0.4 60,247 + EX31_RS14390 0.73 -1.6 60,248 - EX31_RS14390 0.73 -0.7 60,248 - EX31_RS14390 0.73 -0.3 60,308 + EX31_RS14390 0.78 -0.4 60,308 + EX31_RS14390 0.78 -0.2 60,308 + EX31_RS14390 0.78 -0.5 60,308 + EX31_RS14390 0.78 -0.2 60,308 + EX31_RS14390 0.78 -0.2 60,308 + EX31_RS14390 0.78 +0.9 60,308 + EX31_RS14390 0.78 +0.4 60,308 + EX31_RS14390 0.78 +1.2 60,308 + EX31_RS14390 0.78 -0.2 60,309 - EX31_RS14390 0.78 -0.2 60,309 - EX31_RS14390 0.78 -0.9 60,326 + EX31_RS14390 0.80 -0.8
Or see this region's nucleotide sequence