Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS13930 and EX31_RS13935 are separated by 435 nucleotides EX31_RS13935 and EX31_RS13940 are separated by 508 nucleotides
EX31_RS13930: EX31_RS13930 - AI-2E family transporter YdiK, at 46,939 to 48,036
_RS13930
EX31_RS13935: EX31_RS13935 - phosphoenolpyruvate synthase, at 48,472 to 50,847
_RS13935
EX31_RS13940: EX31_RS13940 - kinase/pyrophosphorylase, at 51,356 to 52,177
_RS13940
Position (kb) 48
49
50
51 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 47.473 kb on + strand, within EX31_RS13930 at 47.502 kb on + strand, within EX31_RS13930 at 47.554 kb on - strand, within EX31_RS13930 at 47.554 kb on - strand, within EX31_RS13930 at 47.602 kb on - strand, within EX31_RS13930 at 47.621 kb on + strand, within EX31_RS13930 at 47.621 kb on + strand, within EX31_RS13930 at 47.621 kb on + strand, within EX31_RS13930 at 47.659 kb on + strand, within EX31_RS13930 at 47.660 kb on - strand, within EX31_RS13930 at 47.749 kb on + strand, within EX31_RS13930 at 47.766 kb on + strand, within EX31_RS13930 at 47.817 kb on + strand, within EX31_RS13930 at 47.858 kb on + strand, within EX31_RS13930 at 47.872 kb on - strand, within EX31_RS13930 at 47.872 kb on - strand, within EX31_RS13930 at 47.897 kb on + strand, within EX31_RS13930 at 48.017 kb on + strand at 48.167 kb on + strand at 48.171 kb on - strand at 48.174 kb on + strand at 48.197 kb on - strand at 48.197 kb on - strand at 48.201 kb on - strand at 48.214 kb on + strand at 48.220 kb on + strand at 48.223 kb on + strand at 48.251 kb on + strand at 48.279 kb on + strand at 48.355 kb on + strand at 48.449 kb on - strand at 48.462 kb on - strand at 48.470 kb on - strand at 48.597 kb on + strand at 48.605 kb on + strand at 48.606 kb on - strand at 48.622 kb on - strand at 48.739 kb on - strand, within EX31_RS13935 at 48.739 kb on - strand, within EX31_RS13935 at 48.748 kb on - strand, within EX31_RS13935 at 48.802 kb on + strand, within EX31_RS13935 at 48.816 kb on + strand, within EX31_RS13935 at 49.007 kb on + strand, within EX31_RS13935 at 49.008 kb on - strand, within EX31_RS13935 at 49.008 kb on - strand, within EX31_RS13935 at 49.085 kb on + strand, within EX31_RS13935 at 49.181 kb on + strand, within EX31_RS13935 at 49.181 kb on + strand, within EX31_RS13935 at 49.182 kb on - strand, within EX31_RS13935 at 49.185 kb on - strand, within EX31_RS13935 at 49.185 kb on - strand, within EX31_RS13935 at 49.218 kb on + strand, within EX31_RS13935 at 49.218 kb on + strand, within EX31_RS13935 at 49.218 kb on + strand, within EX31_RS13935 at 49.219 kb on - strand, within EX31_RS13935 at 49.219 kb on - strand, within EX31_RS13935 at 49.219 kb on - strand, within EX31_RS13935 at 49.219 kb on - strand, within EX31_RS13935 at 49.290 kb on + strand, within EX31_RS13935 at 49.300 kb on - strand, within EX31_RS13935 at 49.300 kb on - strand, within EX31_RS13935 at 49.464 kb on - strand, within EX31_RS13935 at 49.667 kb on - strand, within EX31_RS13935 at 49.687 kb on - strand, within EX31_RS13935 at 49.708 kb on - strand, within EX31_RS13935 at 49.790 kb on - strand, within EX31_RS13935 at 49.853 kb on + strand, within EX31_RS13935 at 49.853 kb on + strand, within EX31_RS13935 at 49.854 kb on - strand, within EX31_RS13935 at 49.854 kb on - strand, within EX31_RS13935 at 49.854 kb on - strand, within EX31_RS13935 at 49.854 kb on - strand, within EX31_RS13935 at 49.854 kb on - strand, within EX31_RS13935 at 49.854 kb on - strand, within EX31_RS13935 at 49.854 kb on - strand, within EX31_RS13935 at 49.854 kb on - strand, within EX31_RS13935 at 49.854 kb on - strand, within EX31_RS13935 at 49.854 kb on - strand, within EX31_RS13935 at 49.854 kb on - strand, within EX31_RS13935 at 49.854 kb on - strand, within EX31_RS13935 at 49.907 kb on + strand, within EX31_RS13935 at 49.908 kb on - strand, within EX31_RS13935 at 49.908 kb on - strand, within EX31_RS13935 at 50.040 kb on - strand, within EX31_RS13935 at 50.040 kb on - strand, within EX31_RS13935 at 50.040 kb on - strand, within EX31_RS13935 at 50.040 kb on - strand, within EX31_RS13935 at 50.040 kb on - strand, within EX31_RS13935 at 50.040 kb on - strand, within EX31_RS13935 at 50.040 kb on - strand, within EX31_RS13935 at 50.040 kb on - strand, within EX31_RS13935 at 50.041 kb on + strand, within EX31_RS13935 at 50.041 kb on + strand, within EX31_RS13935 at 50.041 kb on + strand, within EX31_RS13935 at 50.041 kb on + strand, within EX31_RS13935 at 50.041 kb on + strand, within EX31_RS13935 at 50.041 kb on + strand, within EX31_RS13935 at 50.041 kb on + strand, within EX31_RS13935 at 50.041 kb on + strand, within EX31_RS13935 at 50.042 kb on - strand, within EX31_RS13935 at 50.042 kb on - strand, within EX31_RS13935 at 50.042 kb on - strand, within EX31_RS13935 at 50.042 kb on - strand, within EX31_RS13935 at 50.042 kb on - strand, within EX31_RS13935 at 50.042 kb on - strand, within EX31_RS13935 at 50.042 kb on - strand, within EX31_RS13935 at 50.042 kb on - strand, within EX31_RS13935 at 50.082 kb on + strand, within EX31_RS13935 at 50.082 kb on + strand, within EX31_RS13935 at 50.082 kb on + strand, within EX31_RS13935 at 50.082 kb on + strand, within EX31_RS13935 at 50.083 kb on - strand, within EX31_RS13935 at 50.083 kb on - strand, within EX31_RS13935 at 50.083 kb on - strand, within EX31_RS13935 at 50.083 kb on - strand, within EX31_RS13935 at 50.083 kb on - strand, within EX31_RS13935 at 50.083 kb on - strand, within EX31_RS13935 at 50.083 kb on - strand, within EX31_RS13935 at 50.083 kb on - strand, within EX31_RS13935 at 50.083 kb on - strand, within EX31_RS13935 at 50.107 kb on - strand, within EX31_RS13935 at 50.112 kb on + strand, within EX31_RS13935 at 50.112 kb on + strand, within EX31_RS13935 at 50.112 kb on + strand, within EX31_RS13935 at 50.112 kb on + strand, within EX31_RS13935 at 50.113 kb on - strand, within EX31_RS13935 at 50.113 kb on - strand, within EX31_RS13935 at 50.116 kb on + strand, within EX31_RS13935 at 50.116 kb on + strand, within EX31_RS13935 at 50.116 kb on + strand, within EX31_RS13935 at 50.117 kb on - strand, within EX31_RS13935 at 50.117 kb on - strand, within EX31_RS13935 at 50.120 kb on + strand, within EX31_RS13935 at 50.120 kb on + strand, within EX31_RS13935 at 50.171 kb on + strand, within EX31_RS13935 at 50.171 kb on + strand, within EX31_RS13935 at 50.172 kb on - strand, within EX31_RS13935 at 50.341 kb on - strand, within EX31_RS13935 at 50.341 kb on - strand, within EX31_RS13935 at 50.344 kb on + strand, within EX31_RS13935 at 50.345 kb on - strand, within EX31_RS13935 at 50.354 kb on + strand, within EX31_RS13935 at 50.354 kb on + strand, within EX31_RS13935 at 50.377 kb on + strand, within EX31_RS13935 at 50.377 kb on + strand, within EX31_RS13935 at 50.378 kb on - strand, within EX31_RS13935 at 50.378 kb on - strand, within EX31_RS13935 at 50.378 kb on - strand, within EX31_RS13935 at 50.511 kb on - strand, within EX31_RS13935 at 50.575 kb on + strand, within EX31_RS13935 at 50.575 kb on + strand, within EX31_RS13935 at 50.576 kb on - strand, within EX31_RS13935 at 50.576 kb on - strand, within EX31_RS13935 at 50.576 kb on - strand, within EX31_RS13935 at 50.594 kb on - strand, within EX31_RS13935 at 50.594 kb on - strand, within EX31_RS13935 at 50.594 kb on - strand, within EX31_RS13935 at 50.594 kb on - strand, within EX31_RS13935 at 50.594 kb on - strand, within EX31_RS13935 at 50.622 kb on + strand at 50.640 kb on + strand at 50.778 kb on + strand at 50.794 kb on - strand at 50.807 kb on + strand at 50.815 kb on - strand at 50.824 kb on + strand at 50.824 kb on + strand at 50.863 kb on + strand at 50.867 kb on + strand at 50.868 kb on - strand at 50.872 kb on - strand at 50.872 kb on - strand at 50.872 kb on - strand at 50.877 kb on - strand at 50.907 kb on + strand at 50.939 kb on + strand at 50.940 kb on - strand at 51.118 kb on - strand at 51.128 kb on + strand at 51.211 kb on - strand at 51.270 kb on - strand at 51.300 kb on + strand at 51.303 kb on + strand at 51.303 kb on + strand at 51.304 kb on - strand at 51.307 kb on + strand at 51.308 kb on - strand at 51.308 kb on - strand at 51.308 kb on - strand at 51.326 kb on - strand at 51.554 kb on + strand, within EX31_RS13940 at 51.715 kb on + strand, within EX31_RS13940 at 51.715 kb on + strand, within EX31_RS13940 at 51.715 kb on + strand, within EX31_RS13940 at 51.719 kb on + strand, within EX31_RS13940 at 51.720 kb on - strand, within EX31_RS13940 at 51.720 kb on - strand, within EX31_RS13940
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 47,473 + EX31_RS13930 0.49 +0.7 47,502 + EX31_RS13930 0.51 +0.3 47,554 - EX31_RS13930 0.56 -1.2 47,554 - EX31_RS13930 0.56 +0.1 47,602 - EX31_RS13930 0.60 +0.8 47,621 + EX31_RS13930 0.62 +1.5 47,621 + EX31_RS13930 0.62 -0.6 47,621 + EX31_RS13930 0.62 -1.3 47,659 + EX31_RS13930 0.66 +0.7 47,660 - EX31_RS13930 0.66 +1.0 47,749 + EX31_RS13930 0.74 -0.2 47,766 + EX31_RS13930 0.75 +0.1 47,817 + EX31_RS13930 0.80 +0.9 47,858 + EX31_RS13930 0.84 +0.9 47,872 - EX31_RS13930 0.85 -1.1 47,872 - EX31_RS13930 0.85 +1.3 47,897 + EX31_RS13930 0.87 +0.3 48,017 + -0.1 48,167 + +0.2 48,171 - -0.3 48,174 + +0.4 48,197 - +0.5 48,197 - -0.3 48,201 - -0.1 48,214 + +1.6 48,220 + -0.5 48,223 + -0.7 48,251 + +0.6 48,279 + +0.6 48,355 + -0.7 48,449 - +0.5 48,462 - +0.6 48,470 - +0.7 48,597 + +1.3 48,605 + +0.7 48,606 - -0.5 48,622 - +0.5 48,739 - EX31_RS13935 0.11 -0.1 48,739 - EX31_RS13935 0.11 -0.4 48,748 - EX31_RS13935 0.12 +0.8 48,802 + EX31_RS13935 0.14 +0.9 48,816 + EX31_RS13935 0.14 -2.0 49,007 + EX31_RS13935 0.23 +0.6 49,008 - EX31_RS13935 0.23 +0.2 49,008 - EX31_RS13935 0.23 -0.1 49,085 + EX31_RS13935 0.26 -2.4 49,181 + EX31_RS13935 0.30 -1.1 49,181 + EX31_RS13935 0.30 +0.0 49,182 - EX31_RS13935 0.30 -0.7 49,185 - EX31_RS13935 0.30 -1.3 49,185 - EX31_RS13935 0.30 -0.5 49,218 + EX31_RS13935 0.31 -2.0 49,218 + EX31_RS13935 0.31 -0.9 49,218 + EX31_RS13935 0.31 +1.5 49,219 - EX31_RS13935 0.31 +0.3 49,219 - EX31_RS13935 0.31 +0.2 49,219 - EX31_RS13935 0.31 -0.6 49,219 - EX31_RS13935 0.31 -0.1 49,290 + EX31_RS13935 0.34 -0.0 49,300 - EX31_RS13935 0.35 -0.1 49,300 - EX31_RS13935 0.35 +1.9 49,464 - EX31_RS13935 0.42 +0.2 49,667 - EX31_RS13935 0.50 -2.7 49,687 - EX31_RS13935 0.51 -0.0 49,708 - EX31_RS13935 0.52 -2.5 49,790 - EX31_RS13935 0.55 -0.3 49,853 + EX31_RS13935 0.58 -1.3 49,853 + EX31_RS13935 0.58 +0.2 49,854 - EX31_RS13935 0.58 -0.2 49,854 - EX31_RS13935 0.58 -1.1 49,854 - EX31_RS13935 0.58 -0.4 49,854 - EX31_RS13935 0.58 -0.3 49,854 - EX31_RS13935 0.58 +1.9 49,854 - EX31_RS13935 0.58 +0.3 49,854 - EX31_RS13935 0.58 +1.4 49,854 - EX31_RS13935 0.58 +0.7 49,854 - EX31_RS13935 0.58 -0.1 49,854 - EX31_RS13935 0.58 -0.5 49,854 - EX31_RS13935 0.58 -0.4 49,854 - EX31_RS13935 0.58 -0.1 49,907 + EX31_RS13935 0.60 -0.9 49,908 - EX31_RS13935 0.60 +0.1 49,908 - EX31_RS13935 0.60 -0.1 50,040 - EX31_RS13935 0.66 -0.9 50,040 - EX31_RS13935 0.66 +1.3 50,040 - EX31_RS13935 0.66 -1.5 50,040 - EX31_RS13935 0.66 -0.1 50,040 - EX31_RS13935 0.66 -0.4 50,040 - EX31_RS13935 0.66 -1.4 50,040 - EX31_RS13935 0.66 +0.4 50,040 - EX31_RS13935 0.66 +0.1 50,041 + EX31_RS13935 0.66 -0.2 50,041 + EX31_RS13935 0.66 -1.1 50,041 + EX31_RS13935 0.66 +0.8 50,041 + EX31_RS13935 0.66 +0.5 50,041 + EX31_RS13935 0.66 -0.1 50,041 + EX31_RS13935 0.66 -0.6 50,041 + EX31_RS13935 0.66 +0.9 50,041 + EX31_RS13935 0.66 +0.3 50,042 - EX31_RS13935 0.66 +0.6 50,042 - EX31_RS13935 0.66 -0.8 50,042 - EX31_RS13935 0.66 +0.1 50,042 - EX31_RS13935 0.66 -0.3 50,042 - EX31_RS13935 0.66 -0.8 50,042 - EX31_RS13935 0.66 -0.6 50,042 - EX31_RS13935 0.66 +0.1 50,042 - EX31_RS13935 0.66 -1.3 50,082 + EX31_RS13935 0.68 +0.6 50,082 + EX31_RS13935 0.68 -0.1 50,082 + EX31_RS13935 0.68 -0.8 50,082 + EX31_RS13935 0.68 -0.8 50,083 - EX31_RS13935 0.68 -0.9 50,083 - EX31_RS13935 0.68 -0.3 50,083 - EX31_RS13935 0.68 -0.6 50,083 - EX31_RS13935 0.68 +1.7 50,083 - EX31_RS13935 0.68 +0.4 50,083 - EX31_RS13935 0.68 -0.4 50,083 - EX31_RS13935 0.68 -0.1 50,083 - EX31_RS13935 0.68 -0.1 50,083 - EX31_RS13935 0.68 -0.2 50,107 - EX31_RS13935 0.69 +0.3 50,112 + EX31_RS13935 0.69 +0.5 50,112 + EX31_RS13935 0.69 +0.2 50,112 + EX31_RS13935 0.69 -0.6 50,112 + EX31_RS13935 0.69 +1.9 50,113 - EX31_RS13935 0.69 +1.2 50,113 - EX31_RS13935 0.69 -0.0 50,116 + EX31_RS13935 0.69 -0.6 50,116 + EX31_RS13935 0.69 +1.2 50,116 + EX31_RS13935 0.69 -0.8 50,117 - EX31_RS13935 0.69 -0.1 50,117 - EX31_RS13935 0.69 -0.7 50,120 + EX31_RS13935 0.69 -1.0 50,120 + EX31_RS13935 0.69 -0.7 50,171 + EX31_RS13935 0.72 +1.5 50,171 + EX31_RS13935 0.72 +0.2 50,172 - EX31_RS13935 0.72 -1.7 50,341 - EX31_RS13935 0.79 +1.3 50,341 - EX31_RS13935 0.79 +0.9 50,344 + EX31_RS13935 0.79 -1.3 50,345 - EX31_RS13935 0.79 -0.2 50,354 + EX31_RS13935 0.79 -1.1 50,354 + EX31_RS13935 0.79 +2.4 50,377 + EX31_RS13935 0.80 -2.9 50,377 + EX31_RS13935 0.80 -0.2 50,378 - EX31_RS13935 0.80 +0.4 50,378 - EX31_RS13935 0.80 -2.3 50,378 - EX31_RS13935 0.80 +0.1 50,511 - EX31_RS13935 0.86 +1.9 50,575 + EX31_RS13935 0.89 +0.3 50,575 + EX31_RS13935 0.89 -0.4 50,576 - EX31_RS13935 0.89 +0.3 50,576 - EX31_RS13935 0.89 -1.2 50,576 - EX31_RS13935 0.89 +1.2 50,594 - EX31_RS13935 0.89 -0.3 50,594 - EX31_RS13935 0.89 -0.4 50,594 - EX31_RS13935 0.89 -0.6 50,594 - EX31_RS13935 0.89 -1.1 50,594 - EX31_RS13935 0.89 +0.3 50,622 + -0.8 50,640 + -0.3 50,778 + +0.8 50,794 - -0.1 50,807 + +1.9 50,815 - +0.9 50,824 + -1.1 50,824 + -0.1 50,863 + -0.8 50,867 + -0.7 50,868 - +1.6 50,872 - +0.5 50,872 - +0.5 50,872 - +0.4 50,877 - -0.6 50,907 + +0.9 50,939 + -0.1 50,940 - +1.0 51,118 - +1.3 51,128 + -0.4 51,211 - -0.2 51,270 - +0.1 51,300 + +0.5 51,303 + +0.8 51,303 + +0.6 51,304 - +0.2 51,307 + -0.3 51,308 - +0.9 51,308 - -0.1 51,308 - +0.6 51,326 - +0.5 51,554 + EX31_RS13940 0.24 +0.2 51,715 + EX31_RS13940 0.44 +0.3 51,715 + EX31_RS13940 0.44 +1.6 51,715 + EX31_RS13940 0.44 -0.1 51,719 + EX31_RS13940 0.44 -1.6 51,720 - EX31_RS13940 0.44 +0.9 51,720 - EX31_RS13940 0.44 -0.7
Or see this region's nucleotide sequence