Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS12490 and EX31_RS12495 are separated by 16 nucleotides EX31_RS12495 and EX31_RS12500 are separated by 334 nucleotides EX31_RS12500 and EX31_RS12505 are separated by 3 nucleotides EX31_RS12505 and EX31_RS12510 are separated by 162 nucleotides
EX31_RS12490: EX31_RS12490 - 50S ribosomal protein L13, at 164,412 to 164,840
_RS12490
EX31_RS12495: EX31_RS12495 - 30S ribosomal protein S9, at 164,857 to 165,249
_RS12495
EX31_RS12500: EX31_RS12500 - stringent starvation protein A, at 165,584 to 166,225
_RS12500
EX31_RS12505: EX31_RS12505 - ClpXP protease specificity-enhancing factor, at 166,229 to 166,723
_RS12505
EX31_RS12510: EX31_RS12510 - FAD-dependent oxidoreductase, at 166,886 to 168,304
_RS12510
Position (kb) 165
166
167 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 165.261 kb on + strand at 165.342 kb on + strand at 165.343 kb on - strand at 165.429 kb on + strand at 165.510 kb on + strand at 165.558 kb on + strand at 165.558 kb on + strand at 165.563 kb on + strand at 165.567 kb on + strand at 165.567 kb on + strand at 165.657 kb on + strand, within EX31_RS12500 at 165.661 kb on + strand, within EX31_RS12500 at 165.661 kb on + strand, within EX31_RS12500 at 165.661 kb on + strand, within EX31_RS12500 at 165.661 kb on + strand, within EX31_RS12500 at 165.661 kb on + strand, within EX31_RS12500 at 165.661 kb on + strand, within EX31_RS12500 at 165.661 kb on + strand, within EX31_RS12500 at 165.666 kb on + strand, within EX31_RS12500 at 165.733 kb on + strand, within EX31_RS12500 at 165.733 kb on + strand, within EX31_RS12500 at 165.733 kb on + strand, within EX31_RS12500 at 165.749 kb on + strand, within EX31_RS12500 at 165.750 kb on - strand, within EX31_RS12500 at 165.759 kb on + strand, within EX31_RS12500 at 165.759 kb on + strand, within EX31_RS12500 at 165.815 kb on + strand, within EX31_RS12500 at 165.815 kb on + strand, within EX31_RS12500 at 165.914 kb on + strand, within EX31_RS12500 at 165.970 kb on + strand, within EX31_RS12500 at 165.970 kb on + strand, within EX31_RS12500 at 165.970 kb on + strand, within EX31_RS12500 at 165.970 kb on + strand, within EX31_RS12500 at 166.016 kb on + strand, within EX31_RS12500 at 166.016 kb on + strand, within EX31_RS12500 at 166.016 kb on + strand, within EX31_RS12500 at 166.016 kb on + strand, within EX31_RS12500 at 166.016 kb on + strand, within EX31_RS12500 at 166.016 kb on + strand, within EX31_RS12500 at 166.016 kb on + strand, within EX31_RS12500 at 166.016 kb on + strand, within EX31_RS12500 at 166.016 kb on + strand, within EX31_RS12500 at 166.061 kb on + strand, within EX31_RS12500 at 166.061 kb on + strand, within EX31_RS12500 at 166.139 kb on + strand, within EX31_RS12500 at 166.139 kb on + strand, within EX31_RS12500 at 166.139 kb on + strand, within EX31_RS12500 at 166.139 kb on + strand, within EX31_RS12500 at 166.139 kb on + strand, within EX31_RS12500 at 166.139 kb on + strand, within EX31_RS12500 at 166.223 kb on + strand at 166.228 kb on + strand at 166.263 kb on - strand at 166.280 kb on + strand, within EX31_RS12505 at 166.437 kb on + strand, within EX31_RS12505 at 166.437 kb on + strand, within EX31_RS12505 at 166.437 kb on + strand, within EX31_RS12505 at 166.438 kb on - strand, within EX31_RS12505 at 166.444 kb on + strand, within EX31_RS12505 at 166.445 kb on - strand, within EX31_RS12505 at 166.464 kb on + strand, within EX31_RS12505 at 166.471 kb on + strand, within EX31_RS12505 at 166.508 kb on + strand, within EX31_RS12505 at 166.508 kb on + strand, within EX31_RS12505 at 166.508 kb on + strand, within EX31_RS12505 at 166.508 kb on + strand, within EX31_RS12505 at 166.508 kb on + strand, within EX31_RS12505 at 166.509 kb on - strand, within EX31_RS12505 at 166.509 kb on - strand, within EX31_RS12505 at 166.509 kb on - strand, within EX31_RS12505 at 166.557 kb on - strand, within EX31_RS12505 at 166.721 kb on + strand at 166.721 kb on + strand at 166.721 kb on + strand at 166.722 kb on - strand at 166.750 kb on + strand at 166.750 kb on + strand at 166.765 kb on + strand at 166.765 kb on + strand at 166.765 kb on + strand at 166.765 kb on + strand at 166.765 kb on + strand at 166.765 kb on + strand at 166.765 kb on + strand at 166.765 kb on + strand at 166.765 kb on + strand at 166.765 kb on + strand at 166.766 kb on - strand at 166.766 kb on - strand at 166.766 kb on - strand at 166.787 kb on + strand at 166.787 kb on + strand at 166.787 kb on + strand at 166.788 kb on - strand at 166.788 kb on - strand at 166.788 kb on - strand at 166.791 kb on + strand at 166.792 kb on - strand at 166.792 kb on - strand at 166.827 kb on - strand at 166.827 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 165,261 + +1.6 165,342 + +0.2 165,343 - -1.3 165,429 + -1.9 165,510 + -1.8 165,558 + -0.8 165,558 + +0.4 165,563 + -0.9 165,567 + +0.4 165,567 + +0.2 165,657 + EX31_RS12500 0.11 -2.5 165,661 + EX31_RS12500 0.12 -0.1 165,661 + EX31_RS12500 0.12 -0.8 165,661 + EX31_RS12500 0.12 -1.2 165,661 + EX31_RS12500 0.12 -0.9 165,661 + EX31_RS12500 0.12 -1.1 165,661 + EX31_RS12500 0.12 -1.3 165,661 + EX31_RS12500 0.12 -0.5 165,666 + EX31_RS12500 0.13 -0.6 165,733 + EX31_RS12500 0.23 -1.6 165,733 + EX31_RS12500 0.23 -1.1 165,733 + EX31_RS12500 0.23 -1.1 165,749 + EX31_RS12500 0.26 -1.6 165,750 - EX31_RS12500 0.26 -0.3 165,759 + EX31_RS12500 0.27 -1.9 165,759 + EX31_RS12500 0.27 -1.2 165,815 + EX31_RS12500 0.36 -2.0 165,815 + EX31_RS12500 0.36 -1.1 165,914 + EX31_RS12500 0.51 -0.1 165,970 + EX31_RS12500 0.60 -1.7 165,970 + EX31_RS12500 0.60 -0.9 165,970 + EX31_RS12500 0.60 -2.8 165,970 + EX31_RS12500 0.60 -1.7 166,016 + EX31_RS12500 0.67 -1.2 166,016 + EX31_RS12500 0.67 -0.3 166,016 + EX31_RS12500 0.67 -1.4 166,016 + EX31_RS12500 0.67 +0.6 166,016 + EX31_RS12500 0.67 -2.2 166,016 + EX31_RS12500 0.67 -2.4 166,016 + EX31_RS12500 0.67 -1.0 166,016 + EX31_RS12500 0.67 +0.2 166,016 + EX31_RS12500 0.67 -0.1 166,061 + EX31_RS12500 0.74 -1.0 166,061 + EX31_RS12500 0.74 -2.8 166,139 + EX31_RS12500 0.86 -1.1 166,139 + EX31_RS12500 0.86 -0.9 166,139 + EX31_RS12500 0.86 -1.2 166,139 + EX31_RS12500 0.86 -1.7 166,139 + EX31_RS12500 0.86 -1.1 166,139 + EX31_RS12500 0.86 -0.7 166,223 + -1.3 166,228 + +0.6 166,263 - +0.2 166,280 + EX31_RS12505 0.10 +0.4 166,437 + EX31_RS12505 0.42 -0.9 166,437 + EX31_RS12505 0.42 -0.0 166,437 + EX31_RS12505 0.42 -0.3 166,438 - EX31_RS12505 0.42 +0.5 166,444 + EX31_RS12505 0.43 -0.3 166,445 - EX31_RS12505 0.44 +0.6 166,464 + EX31_RS12505 0.47 -0.6 166,471 + EX31_RS12505 0.49 -0.6 166,508 + EX31_RS12505 0.56 -0.7 166,508 + EX31_RS12505 0.56 -0.2 166,508 + EX31_RS12505 0.56 -0.3 166,508 + EX31_RS12505 0.56 -0.3 166,508 + EX31_RS12505 0.56 -0.9 166,509 - EX31_RS12505 0.57 -0.8 166,509 - EX31_RS12505 0.57 +0.7 166,509 - EX31_RS12505 0.57 -0.7 166,557 - EX31_RS12505 0.66 -0.3 166,721 + -0.8 166,721 + -0.7 166,721 + -0.6 166,722 - +0.5 166,750 + +0.0 166,750 + -1.2 166,765 + +0.1 166,765 + -0.5 166,765 + -0.2 166,765 + +0.7 166,765 + +0.2 166,765 + +1.1 166,765 + +0.1 166,765 + -0.2 166,765 + +0.2 166,765 + -0.8 166,766 - +1.5 166,766 - -0.2 166,766 - -1.3 166,787 + +0.4 166,787 + -0.3 166,787 + -0.4 166,788 - -0.4 166,788 - +1.6 166,788 - +0.5 166,791 + -0.2 166,792 - -0.5 166,792 - +0.1 166,827 - +0.9 166,827 - +0.2
Or see this region's nucleotide sequence