Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS11950 and EX31_RS11955 are separated by 77 nucleotides EX31_RS11955 and EX31_RS11960 overlap by 19 nucleotides EX31_RS11960 and EX31_RS11965 are separated by 6 nucleotides EX31_RS11965 and EX31_RS11970 are separated by 101 nucleotides
EX31_RS11950: EX31_RS11950 - MFS transporter, at 51,797 to 53,212
_RS11950
EX31_RS11955: EX31_RS11955 - MmcQ/YjbR family DNA-binding protein, at 53,290 to 53,643
_RS11955
EX31_RS11960: EX31_RS11960 - pyridoxamine 5'-phosphate oxidase, at 53,625 to 54,545
_RS11960
EX31_RS11965: EX31_RS11965 - glutathione S-transferase, at 54,552 to 55,172
_RS11965
EX31_RS11970: EX31_RS11970 - LysR family transcriptional regulator, at 55,274 to 56,191
_RS11970
Position (kb) 53
54
55 Strain fitness (log2 ratio) -2
-1
0
1
2 at 52.706 kb on + strand, within EX31_RS11950 at 52.706 kb on + strand, within EX31_RS11950 at 52.706 kb on + strand, within EX31_RS11950 at 52.706 kb on + strand, within EX31_RS11950 at 52.707 kb on - strand, within EX31_RS11950 at 52.707 kb on - strand, within EX31_RS11950 at 52.719 kb on + strand, within EX31_RS11950 at 52.719 kb on + strand, within EX31_RS11950 at 52.719 kb on + strand, within EX31_RS11950 at 52.719 kb on + strand, within EX31_RS11950 at 52.719 kb on + strand, within EX31_RS11950 at 52.720 kb on - strand, within EX31_RS11950 at 52.720 kb on - strand, within EX31_RS11950 at 52.720 kb on - strand, within EX31_RS11950 at 52.748 kb on - strand, within EX31_RS11950 at 52.862 kb on + strand, within EX31_RS11950 at 52.863 kb on - strand, within EX31_RS11950 at 52.904 kb on + strand, within EX31_RS11950 at 52.904 kb on + strand, within EX31_RS11950 at 52.905 kb on - strand, within EX31_RS11950 at 52.905 kb on - strand, within EX31_RS11950 at 52.905 kb on - strand, within EX31_RS11950 at 52.906 kb on + strand, within EX31_RS11950 at 52.907 kb on - strand, within EX31_RS11950 at 52.914 kb on - strand, within EX31_RS11950 at 52.915 kb on + strand, within EX31_RS11950 at 52.916 kb on - strand, within EX31_RS11950 at 52.916 kb on - strand, within EX31_RS11950 at 52.932 kb on + strand, within EX31_RS11950 at 52.933 kb on - strand, within EX31_RS11950 at 52.966 kb on + strand, within EX31_RS11950 at 52.966 kb on + strand, within EX31_RS11950 at 52.966 kb on + strand, within EX31_RS11950 at 52.966 kb on + strand, within EX31_RS11950 at 52.966 kb on + strand, within EX31_RS11950 at 52.966 kb on + strand, within EX31_RS11950 at 52.966 kb on + strand, within EX31_RS11950 at 52.966 kb on + strand, within EX31_RS11950 at 52.966 kb on + strand, within EX31_RS11950 at 52.967 kb on - strand, within EX31_RS11950 at 52.967 kb on - strand, within EX31_RS11950 at 52.985 kb on + strand, within EX31_RS11950 at 52.985 kb on + strand, within EX31_RS11950 at 52.985 kb on + strand, within EX31_RS11950 at 52.985 kb on + strand, within EX31_RS11950 at 52.986 kb on - strand, within EX31_RS11950 at 52.986 kb on - strand, within EX31_RS11950 at 52.986 kb on - strand, within EX31_RS11950 at 52.988 kb on - strand, within EX31_RS11950 at 53.019 kb on + strand, within EX31_RS11950 at 53.044 kb on + strand, within EX31_RS11950 at 53.045 kb on - strand, within EX31_RS11950 at 53.045 kb on - strand, within EX31_RS11950 at 53.083 kb on + strand at 53.091 kb on + strand at 53.092 kb on - strand at 53.092 kb on - strand at 53.097 kb on - strand at 53.097 kb on - strand at 53.097 kb on - strand at 53.203 kb on - strand at 53.203 kb on - strand at 53.210 kb on + strand at 53.210 kb on + strand at 53.210 kb on + strand at 53.229 kb on - strand at 53.255 kb on + strand at 53.255 kb on + strand at 53.255 kb on + strand at 53.255 kb on + strand at 53.256 kb on - strand at 53.256 kb on - strand at 53.256 kb on - strand at 53.256 kb on - strand at 53.256 kb on - strand at 53.256 kb on - strand at 53.259 kb on + strand at 53.259 kb on + strand at 53.259 kb on + strand at 53.259 kb on + strand at 53.259 kb on + strand at 53.259 kb on + strand at 53.259 kb on + strand at 53.260 kb on - strand at 53.260 kb on - strand at 53.260 kb on - strand at 53.272 kb on - strand at 53.272 kb on - strand at 53.272 kb on - strand at 53.314 kb on + strand at 53.314 kb on + strand at 53.314 kb on + strand at 53.315 kb on - strand at 53.315 kb on - strand at 53.315 kb on - strand at 53.315 kb on - strand at 53.315 kb on - strand at 53.315 kb on - strand at 53.315 kb on - strand at 53.315 kb on - strand at 53.376 kb on - strand, within EX31_RS11955 at 53.376 kb on - strand, within EX31_RS11955 at 53.430 kb on + strand, within EX31_RS11955 at 53.430 kb on + strand, within EX31_RS11955 at 53.431 kb on - strand, within EX31_RS11955 at 53.468 kb on + strand, within EX31_RS11955 at 53.469 kb on - strand, within EX31_RS11955 at 53.566 kb on + strand, within EX31_RS11955 at 53.566 kb on + strand, within EX31_RS11955 at 53.566 kb on + strand, within EX31_RS11955 at 53.567 kb on - strand, within EX31_RS11955 at 53.567 kb on - strand, within EX31_RS11955 at 53.567 kb on - strand, within EX31_RS11955 at 53.567 kb on - strand, within EX31_RS11955 at 53.567 kb on - strand, within EX31_RS11955 at 53.570 kb on - strand, within EX31_RS11955 at 53.587 kb on + strand, within EX31_RS11955 at 53.588 kb on - strand, within EX31_RS11955 at 53.755 kb on + strand, within EX31_RS11960 at 53.756 kb on - strand, within EX31_RS11960 at 53.783 kb on + strand, within EX31_RS11960 at 53.852 kb on + strand, within EX31_RS11960 at 53.897 kb on + strand, within EX31_RS11960 at 53.897 kb on + strand, within EX31_RS11960 at 53.898 kb on - strand, within EX31_RS11960 at 53.903 kb on + strand, within EX31_RS11960 at 53.903 kb on + strand, within EX31_RS11960 at 53.903 kb on + strand, within EX31_RS11960 at 53.904 kb on - strand, within EX31_RS11960 at 53.904 kb on - strand, within EX31_RS11960 at 53.904 kb on - strand, within EX31_RS11960 at 53.904 kb on - strand, within EX31_RS11960 at 53.905 kb on + strand, within EX31_RS11960 at 53.905 kb on + strand, within EX31_RS11960 at 53.906 kb on - strand, within EX31_RS11960 at 53.906 kb on - strand, within EX31_RS11960 at 54.075 kb on - strand, within EX31_RS11960 at 54.075 kb on - strand, within EX31_RS11960 at 54.127 kb on - strand, within EX31_RS11960 at 54.127 kb on - strand, within EX31_RS11960 at 54.127 kb on - strand, within EX31_RS11960 at 54.134 kb on + strand, within EX31_RS11960 at 54.134 kb on + strand, within EX31_RS11960 at 54.135 kb on - strand, within EX31_RS11960 at 54.136 kb on + strand, within EX31_RS11960 at 54.136 kb on + strand, within EX31_RS11960 at 54.136 kb on + strand, within EX31_RS11960 at 54.136 kb on + strand, within EX31_RS11960 at 54.137 kb on - strand, within EX31_RS11960 at 54.137 kb on - strand, within EX31_RS11960 at 54.137 kb on - strand, within EX31_RS11960 at 54.137 kb on - strand, within EX31_RS11960 at 54.137 kb on - strand, within EX31_RS11960 at 54.137 kb on - strand, within EX31_RS11960 at 54.137 kb on - strand, within EX31_RS11960 at 54.137 kb on - strand, within EX31_RS11960 at 54.149 kb on + strand, within EX31_RS11960 at 54.149 kb on + strand, within EX31_RS11960 at 54.150 kb on - strand, within EX31_RS11960 at 54.276 kb on + strand, within EX31_RS11960 at 54.344 kb on + strand, within EX31_RS11960 at 54.350 kb on + strand, within EX31_RS11960 at 54.350 kb on + strand, within EX31_RS11960 at 54.414 kb on - strand, within EX31_RS11960 at 54.421 kb on + strand, within EX31_RS11960 at 54.593 kb on + strand at 54.685 kb on + strand, within EX31_RS11965 at 54.685 kb on + strand, within EX31_RS11965 at 54.685 kb on + strand, within EX31_RS11965 at 54.685 kb on + strand, within EX31_RS11965 at 54.685 kb on + strand, within EX31_RS11965 at 54.685 kb on + strand, within EX31_RS11965 at 54.685 kb on + strand, within EX31_RS11965 at 54.685 kb on + strand, within EX31_RS11965 at 54.685 kb on + strand, within EX31_RS11965 at 54.685 kb on + strand, within EX31_RS11965 at 54.686 kb on - strand, within EX31_RS11965 at 54.686 kb on - strand, within EX31_RS11965 at 54.686 kb on - strand, within EX31_RS11965 at 54.686 kb on - strand, within EX31_RS11965 at 54.686 kb on - strand, within EX31_RS11965 at 54.686 kb on - strand, within EX31_RS11965 at 54.691 kb on + strand, within EX31_RS11965 at 54.691 kb on + strand, within EX31_RS11965 at 54.691 kb on + strand, within EX31_RS11965 at 54.691 kb on + strand, within EX31_RS11965 at 54.691 kb on + strand, within EX31_RS11965 at 54.692 kb on - strand, within EX31_RS11965 at 54.692 kb on - strand, within EX31_RS11965 at 54.944 kb on - strand, within EX31_RS11965 at 54.959 kb on - strand, within EX31_RS11965 at 55.032 kb on + strand, within EX31_RS11965 at 55.033 kb on - strand, within EX31_RS11965 at 55.033 kb on - strand, within EX31_RS11965 at 55.033 kb on - strand, within EX31_RS11965 at 55.119 kb on + strand at 55.120 kb on - strand at 55.120 kb on - strand at 55.120 kb on - strand at 55.120 kb on - strand at 55.154 kb on - strand at 55.154 kb on - strand at 55.154 kb on - strand at 55.186 kb on + strand at 55.187 kb on - strand at 55.206 kb on + strand at 55.207 kb on - strand at 55.311 kb on + strand at 55.311 kb on + strand at 55.319 kb on + strand at 55.319 kb on + strand at 55.345 kb on + strand at 55.520 kb on - strand, within EX31_RS11970
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 52,706 + EX31_RS11950 0.64 +1.4 52,706 + EX31_RS11950 0.64 -0.0 52,706 + EX31_RS11950 0.64 +1.0 52,706 + EX31_RS11950 0.64 -0.6 52,707 - EX31_RS11950 0.64 +0.6 52,707 - EX31_RS11950 0.64 +0.9 52,719 + EX31_RS11950 0.65 +0.7 52,719 + EX31_RS11950 0.65 +0.3 52,719 + EX31_RS11950 0.65 -0.0 52,719 + EX31_RS11950 0.65 -0.1 52,719 + EX31_RS11950 0.65 +0.2 52,720 - EX31_RS11950 0.65 -0.2 52,720 - EX31_RS11950 0.65 +0.5 52,720 - EX31_RS11950 0.65 -0.6 52,748 - EX31_RS11950 0.67 -0.1 52,862 + EX31_RS11950 0.75 -0.3 52,863 - EX31_RS11950 0.75 +0.2 52,904 + EX31_RS11950 0.78 -0.3 52,904 + EX31_RS11950 0.78 -0.4 52,905 - EX31_RS11950 0.78 +0.8 52,905 - EX31_RS11950 0.78 -0.0 52,905 - EX31_RS11950 0.78 -0.0 52,906 + EX31_RS11950 0.78 -0.9 52,907 - EX31_RS11950 0.78 +0.0 52,914 - EX31_RS11950 0.79 -1.1 52,915 + EX31_RS11950 0.79 -0.3 52,916 - EX31_RS11950 0.79 -1.5 52,916 - EX31_RS11950 0.79 -0.1 52,932 + EX31_RS11950 0.80 +0.1 52,933 - EX31_RS11950 0.80 +0.2 52,966 + EX31_RS11950 0.83 +0.3 52,966 + EX31_RS11950 0.83 +1.9 52,966 + EX31_RS11950 0.83 +0.1 52,966 + EX31_RS11950 0.83 -1.5 52,966 + EX31_RS11950 0.83 -0.6 52,966 + EX31_RS11950 0.83 -1.2 52,966 + EX31_RS11950 0.83 -0.2 52,966 + EX31_RS11950 0.83 +0.4 52,966 + EX31_RS11950 0.83 -0.4 52,967 - EX31_RS11950 0.83 +0.2 52,967 - EX31_RS11950 0.83 +0.3 52,985 + EX31_RS11950 0.84 +0.2 52,985 + EX31_RS11950 0.84 -0.5 52,985 + EX31_RS11950 0.84 +0.2 52,985 + EX31_RS11950 0.84 +0.1 52,986 - EX31_RS11950 0.84 -1.4 52,986 - EX31_RS11950 0.84 +0.4 52,986 - EX31_RS11950 0.84 +0.2 52,988 - EX31_RS11950 0.84 -0.4 53,019 + EX31_RS11950 0.86 +0.7 53,044 + EX31_RS11950 0.88 -0.1 53,045 - EX31_RS11950 0.88 +0.5 53,045 - EX31_RS11950 0.88 +0.7 53,083 + +0.6 53,091 + +0.9 53,092 - -1.4 53,092 - -1.1 53,097 - +0.1 53,097 - -0.5 53,097 - -0.2 53,203 - -0.9 53,203 - +0.7 53,210 + -1.3 53,210 + -0.9 53,210 + -0.3 53,229 - +0.4 53,255 + -0.8 53,255 + -1.2 53,255 + -1.8 53,255 + -0.2 53,256 - +0.9 53,256 - +0.8 53,256 - -0.2 53,256 - +0.5 53,256 - -0.0 53,256 - +0.6 53,259 + -0.3 53,259 + -0.1 53,259 + -1.0 53,259 + +0.1 53,259 + -2.2 53,259 + +1.2 53,259 + -0.5 53,260 - +1.7 53,260 - +1.0 53,260 - +0.3 53,272 - -0.9 53,272 - -1.5 53,272 - +0.5 53,314 + -0.2 53,314 + -0.6 53,314 + -0.0 53,315 - +0.5 53,315 - -0.7 53,315 - +1.4 53,315 - -0.5 53,315 - +0.3 53,315 - +0.4 53,315 - +0.8 53,315 - +0.6 53,376 - EX31_RS11955 0.24 +2.3 53,376 - EX31_RS11955 0.24 -1.8 53,430 + EX31_RS11955 0.40 -0.0 53,430 + EX31_RS11955 0.40 -1.0 53,431 - EX31_RS11955 0.40 +1.7 53,468 + EX31_RS11955 0.50 +0.1 53,469 - EX31_RS11955 0.51 -0.4 53,566 + EX31_RS11955 0.78 +0.9 53,566 + EX31_RS11955 0.78 +0.4 53,566 + EX31_RS11955 0.78 +0.4 53,567 - EX31_RS11955 0.78 +0.2 53,567 - EX31_RS11955 0.78 +0.8 53,567 - EX31_RS11955 0.78 +1.0 53,567 - EX31_RS11955 0.78 +1.3 53,567 - EX31_RS11955 0.78 +0.4 53,570 - EX31_RS11955 0.79 +0.2 53,587 + EX31_RS11955 0.84 -0.7 53,588 - EX31_RS11955 0.84 +0.5 53,755 + EX31_RS11960 0.14 +0.3 53,756 - EX31_RS11960 0.14 +0.2 53,783 + EX31_RS11960 0.17 -0.3 53,852 + EX31_RS11960 0.25 -0.7 53,897 + EX31_RS11960 0.30 +0.9 53,897 + EX31_RS11960 0.30 +1.1 53,898 - EX31_RS11960 0.30 +1.4 53,903 + EX31_RS11960 0.30 -0.6 53,903 + EX31_RS11960 0.30 -1.6 53,903 + EX31_RS11960 0.30 -0.8 53,904 - EX31_RS11960 0.30 -0.8 53,904 - EX31_RS11960 0.30 +0.9 53,904 - EX31_RS11960 0.30 +1.5 53,904 - EX31_RS11960 0.30 +0.3 53,905 + EX31_RS11960 0.30 +0.6 53,905 + EX31_RS11960 0.30 -0.4 53,906 - EX31_RS11960 0.31 +0.4 53,906 - EX31_RS11960 0.31 -0.1 54,075 - EX31_RS11960 0.49 +0.1 54,075 - EX31_RS11960 0.49 -0.5 54,127 - EX31_RS11960 0.55 -0.7 54,127 - EX31_RS11960 0.55 -2.2 54,127 - EX31_RS11960 0.55 +0.1 54,134 + EX31_RS11960 0.55 +1.4 54,134 + EX31_RS11960 0.55 +1.2 54,135 - EX31_RS11960 0.55 +0.0 54,136 + EX31_RS11960 0.55 -0.9 54,136 + EX31_RS11960 0.55 +0.8 54,136 + EX31_RS11960 0.55 -0.9 54,136 + EX31_RS11960 0.55 +0.8 54,137 - EX31_RS11960 0.56 -1.2 54,137 - EX31_RS11960 0.56 -1.2 54,137 - EX31_RS11960 0.56 -0.0 54,137 - EX31_RS11960 0.56 -0.8 54,137 - EX31_RS11960 0.56 +0.8 54,137 - EX31_RS11960 0.56 +0.3 54,137 - EX31_RS11960 0.56 -0.0 54,137 - EX31_RS11960 0.56 -0.5 54,149 + EX31_RS11960 0.57 -0.5 54,149 + EX31_RS11960 0.57 -0.2 54,150 - EX31_RS11960 0.57 +0.2 54,276 + EX31_RS11960 0.71 -0.3 54,344 + EX31_RS11960 0.78 -0.1 54,350 + EX31_RS11960 0.79 +0.3 54,350 + EX31_RS11960 0.79 -0.3 54,414 - EX31_RS11960 0.86 +0.0 54,421 + EX31_RS11960 0.86 -0.3 54,593 + -1.4 54,685 + EX31_RS11965 0.21 +0.2 54,685 + EX31_RS11965 0.21 -1.0 54,685 + EX31_RS11965 0.21 -0.5 54,685 + EX31_RS11965 0.21 -0.7 54,685 + EX31_RS11965 0.21 -0.1 54,685 + EX31_RS11965 0.21 -0.5 54,685 + EX31_RS11965 0.21 +0.7 54,685 + EX31_RS11965 0.21 -1.3 54,685 + EX31_RS11965 0.21 -0.2 54,685 + EX31_RS11965 0.21 -1.1 54,686 - EX31_RS11965 0.22 +1.3 54,686 - EX31_RS11965 0.22 -0.9 54,686 - EX31_RS11965 0.22 -0.5 54,686 - EX31_RS11965 0.22 +1.4 54,686 - EX31_RS11965 0.22 +0.3 54,686 - EX31_RS11965 0.22 -0.5 54,691 + EX31_RS11965 0.22 +0.8 54,691 + EX31_RS11965 0.22 +0.6 54,691 + EX31_RS11965 0.22 -0.9 54,691 + EX31_RS11965 0.22 +0.4 54,691 + EX31_RS11965 0.22 +1.0 54,692 - EX31_RS11965 0.23 -0.4 54,692 - EX31_RS11965 0.23 -0.4 54,944 - EX31_RS11965 0.63 +0.5 54,959 - EX31_RS11965 0.66 -0.2 55,032 + EX31_RS11965 0.77 +0.0 55,033 - EX31_RS11965 0.77 -0.2 55,033 - EX31_RS11965 0.77 +2.1 55,033 - EX31_RS11965 0.77 -0.0 55,119 + -0.3 55,120 - -0.1 55,120 - +0.4 55,120 - -1.3 55,120 - -0.4 55,154 - -1.1 55,154 - -0.2 55,154 - -0.3 55,186 + -0.6 55,187 - -0.4 55,206 + +0.6 55,207 - +0.0 55,311 + +0.1 55,311 + +0.4 55,319 + -0.7 55,319 + -0.4 55,345 + +2.6 55,520 - EX31_RS11970 0.27 +0.4
Or see this region's nucleotide sequence