Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS11345 and EX31_RS11350 are separated by 325 nucleotides EX31_RS11350 and EX31_RS11355 are separated by 10 nucleotides EX31_RS11355 and EX31_RS11360 are separated by 46 nucleotides
EX31_RS11345: EX31_RS11345 - flagellar type III secretion system protein FlhB, at 200,331 to 201,485
_RS11345
EX31_RS11350: EX31_RS11350 - protein phosphatase CheZ, at 201,811 to 202,455
_RS11350
EX31_RS11355: EX31_RS11355 - chemotaxis protein CheY, at 202,466 to 202,855
_RS11355
EX31_RS11360: EX31_RS11360 - chemotaxis response regulator protein-glutamate methylesterase, at 202,902 to 203,951
_RS11360
Position (kb) 202
203 Strain fitness (log2 ratio) -2
-1
0
1
2 at 201.517 kb on + strand at 201.517 kb on + strand at 201.518 kb on - strand at 201.568 kb on + strand at 201.568 kb on + strand at 201.569 kb on - strand at 201.569 kb on - strand at 201.569 kb on - strand at 201.569 kb on - strand at 201.569 kb on - strand at 201.569 kb on - strand at 201.569 kb on - strand at 201.569 kb on - strand at 201.569 kb on - strand at 201.606 kb on - strand at 201.638 kb on + strand at 201.660 kb on + strand at 201.661 kb on - strand at 201.661 kb on - strand at 201.696 kb on + strand at 201.716 kb on + strand at 201.791 kb on - strand at 201.842 kb on - strand at 202.074 kb on + strand, within EX31_RS11350 at 202.107 kb on - strand, within EX31_RS11350 at 202.286 kb on + strand, within EX31_RS11350 at 202.287 kb on - strand, within EX31_RS11350 at 202.287 kb on - strand, within EX31_RS11350 at 202.287 kb on - strand, within EX31_RS11350 at 202.287 kb on - strand, within EX31_RS11350 at 202.287 kb on - strand, within EX31_RS11350 at 202.287 kb on - strand, within EX31_RS11350 at 202.287 kb on - strand, within EX31_RS11350 at 202.287 kb on - strand, within EX31_RS11350 at 202.287 kb on - strand, within EX31_RS11350 at 202.377 kb on + strand, within EX31_RS11350 at 202.378 kb on - strand, within EX31_RS11350 at 202.444 kb on + strand at 202.445 kb on - strand at 202.445 kb on - strand at 202.445 kb on - strand at 202.456 kb on - strand at 202.456 kb on - strand at 202.456 kb on - strand at 202.457 kb on + strand at 202.457 kb on + strand at 202.458 kb on - strand at 202.458 kb on - strand at 202.458 kb on - strand at 202.458 kb on - strand at 202.458 kb on - strand at 202.458 kb on - strand at 202.458 kb on - strand at 202.458 kb on - strand at 202.467 kb on + strand at 202.468 kb on - strand at 202.468 kb on - strand at 202.523 kb on - strand, within EX31_RS11355 at 202.539 kb on + strand, within EX31_RS11355 at 202.540 kb on - strand, within EX31_RS11355 at 202.811 kb on - strand, within EX31_RS11355 at 202.861 kb on - strand at 202.864 kb on + strand at 202.865 kb on - strand at 202.865 kb on - strand at 202.865 kb on - strand at 202.865 kb on - strand at 202.907 kb on + strand at 202.907 kb on + strand at 202.907 kb on + strand at 202.908 kb on - strand at 202.911 kb on + strand at 202.911 kb on + strand at 202.912 kb on - strand at 202.912 kb on - strand at 202.912 kb on - strand at 202.912 kb on - strand at 202.912 kb on - strand at 202.930 kb on - strand at 202.930 kb on - strand at 203.013 kb on - strand, within EX31_RS11360 at 203.054 kb on - strand, within EX31_RS11360 at 203.054 kb on - strand, within EX31_RS11360 at 203.054 kb on - strand, within EX31_RS11360 at 203.054 kb on - strand, within EX31_RS11360 at 203.163 kb on + strand, within EX31_RS11360 at 203.164 kb on - strand, within EX31_RS11360 at 203.217 kb on - strand, within EX31_RS11360 at 203.217 kb on - strand, within EX31_RS11360 at 203.217 kb on - strand, within EX31_RS11360 at 203.217 kb on - strand, within EX31_RS11360 at 203.224 kb on + strand, within EX31_RS11360 at 203.225 kb on - strand, within EX31_RS11360 at 203.252 kb on + strand, within EX31_RS11360 at 203.252 kb on + strand, within EX31_RS11360 at 203.252 kb on + strand, within EX31_RS11360 at 203.252 kb on + strand, within EX31_RS11360 at 203.252 kb on + strand, within EX31_RS11360 at 203.253 kb on - strand, within EX31_RS11360 at 203.253 kb on - strand, within EX31_RS11360 at 203.253 kb on - strand, within EX31_RS11360 at 203.253 kb on - strand, within EX31_RS11360 at 203.253 kb on - strand, within EX31_RS11360 at 203.253 kb on - strand, within EX31_RS11360 at 203.253 kb on - strand, within EX31_RS11360 at 203.275 kb on + strand, within EX31_RS11360 at 203.276 kb on - strand, within EX31_RS11360 at 203.276 kb on - strand, within EX31_RS11360 at 203.276 kb on - strand, within EX31_RS11360 at 203.276 kb on - strand, within EX31_RS11360 at 203.276 kb on - strand, within EX31_RS11360 at 203.276 kb on - strand, within EX31_RS11360 at 203.276 kb on - strand, within EX31_RS11360 at 203.276 kb on - strand, within EX31_RS11360 at 203.276 kb on - strand, within EX31_RS11360 at 203.276 kb on - strand, within EX31_RS11360 at 203.334 kb on + strand, within EX31_RS11360 at 203.334 kb on + strand, within EX31_RS11360 at 203.334 kb on + strand, within EX31_RS11360 at 203.334 kb on + strand, within EX31_RS11360 at 203.335 kb on - strand, within EX31_RS11360 at 203.335 kb on - strand, within EX31_RS11360 at 203.335 kb on - strand, within EX31_RS11360 at 203.335 kb on - strand, within EX31_RS11360 at 203.381 kb on + strand, within EX31_RS11360 at 203.381 kb on + strand, within EX31_RS11360 at 203.382 kb on - strand, within EX31_RS11360 at 203.382 kb on - strand, within EX31_RS11360 at 203.382 kb on - strand, within EX31_RS11360 at 203.382 kb on - strand, within EX31_RS11360 at 203.382 kb on - strand, within EX31_RS11360 at 203.382 kb on - strand, within EX31_RS11360 at 203.382 kb on - strand, within EX31_RS11360 at 203.382 kb on - strand, within EX31_RS11360 at 203.504 kb on + strand, within EX31_RS11360 at 203.606 kb on + strand, within EX31_RS11360 at 203.607 kb on - strand, within EX31_RS11360 at 203.607 kb on - strand, within EX31_RS11360 at 203.607 kb on - strand, within EX31_RS11360 at 203.607 kb on - strand, within EX31_RS11360 at 203.619 kb on - strand, within EX31_RS11360 at 203.676 kb on - strand, within EX31_RS11360 at 203.676 kb on - strand, within EX31_RS11360 at 203.676 kb on - strand, within EX31_RS11360
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 201,517 + -1.4 201,517 + +0.4 201,518 - +0.9 201,568 + +0.4 201,568 + -2.1 201,569 - +0.9 201,569 - -0.3 201,569 - -0.8 201,569 - +1.0 201,569 - -0.9 201,569 - +0.4 201,569 - +0.8 201,569 - -0.4 201,569 - -0.6 201,606 - -0.3 201,638 + -1.1 201,660 + -1.9 201,661 - -0.3 201,661 - +0.4 201,696 + -0.2 201,716 + -0.6 201,791 - +0.1 201,842 - +0.1 202,074 + EX31_RS11350 0.41 -1.4 202,107 - EX31_RS11350 0.46 -1.0 202,286 + EX31_RS11350 0.74 -0.9 202,287 - EX31_RS11350 0.74 -1.8 202,287 - EX31_RS11350 0.74 +1.4 202,287 - EX31_RS11350 0.74 -2.1 202,287 - EX31_RS11350 0.74 -1.1 202,287 - EX31_RS11350 0.74 +0.9 202,287 - EX31_RS11350 0.74 -1.5 202,287 - EX31_RS11350 0.74 +2.5 202,287 - EX31_RS11350 0.74 -2.3 202,287 - EX31_RS11350 0.74 -0.2 202,377 + EX31_RS11350 0.88 -0.1 202,378 - EX31_RS11350 0.88 +0.2 202,444 + +1.2 202,445 - -0.1 202,445 - +0.9 202,445 - -0.5 202,456 - +0.5 202,456 - +0.4 202,456 - -0.2 202,457 + -1.0 202,457 + -1.6 202,458 - +0.2 202,458 - -1.5 202,458 - +0.6 202,458 - -1.0 202,458 - -1.5 202,458 - +0.1 202,458 - -1.1 202,458 - -1.2 202,467 + -0.2 202,468 - -0.2 202,468 - -0.1 202,523 - EX31_RS11355 0.15 -0.9 202,539 + EX31_RS11355 0.19 -0.9 202,540 - EX31_RS11355 0.19 -1.5 202,811 - EX31_RS11355 0.88 -1.4 202,861 - -1.2 202,864 + +1.1 202,865 - +0.4 202,865 - -0.1 202,865 - -2.6 202,865 - -1.0 202,907 + +0.3 202,907 + -0.2 202,907 + +0.8 202,908 - -0.3 202,911 + +1.9 202,911 + -0.3 202,912 - +0.4 202,912 - +0.4 202,912 - +1.8 202,912 - -0.9 202,912 - +0.2 202,930 - +0.8 202,930 - +0.5 203,013 - EX31_RS11360 0.11 +1.2 203,054 - EX31_RS11360 0.14 +0.5 203,054 - EX31_RS11360 0.14 -1.3 203,054 - EX31_RS11360 0.14 +0.9 203,054 - EX31_RS11360 0.14 +1.2 203,163 + EX31_RS11360 0.25 -2.1 203,164 - EX31_RS11360 0.25 -0.6 203,217 - EX31_RS11360 0.30 -0.9 203,217 - EX31_RS11360 0.30 -2.0 203,217 - EX31_RS11360 0.30 -2.0 203,217 - EX31_RS11360 0.30 +1.3 203,224 + EX31_RS11360 0.31 +1.9 203,225 - EX31_RS11360 0.31 -1.2 203,252 + EX31_RS11360 0.33 -2.3 203,252 + EX31_RS11360 0.33 -0.5 203,252 + EX31_RS11360 0.33 +0.0 203,252 + EX31_RS11360 0.33 +0.4 203,252 + EX31_RS11360 0.33 -0.3 203,253 - EX31_RS11360 0.33 +0.4 203,253 - EX31_RS11360 0.33 -1.1 203,253 - EX31_RS11360 0.33 -0.4 203,253 - EX31_RS11360 0.33 +0.2 203,253 - EX31_RS11360 0.33 -0.3 203,253 - EX31_RS11360 0.33 -1.6 203,253 - EX31_RS11360 0.33 -1.5 203,275 + EX31_RS11360 0.36 +0.9 203,276 - EX31_RS11360 0.36 +0.6 203,276 - EX31_RS11360 0.36 -0.1 203,276 - EX31_RS11360 0.36 +0.1 203,276 - EX31_RS11360 0.36 +1.4 203,276 - EX31_RS11360 0.36 -1.1 203,276 - EX31_RS11360 0.36 -0.1 203,276 - EX31_RS11360 0.36 -1.0 203,276 - EX31_RS11360 0.36 -1.0 203,276 - EX31_RS11360 0.36 +0.1 203,276 - EX31_RS11360 0.36 -0.8 203,334 + EX31_RS11360 0.41 -1.7 203,334 + EX31_RS11360 0.41 +0.2 203,334 + EX31_RS11360 0.41 -0.9 203,334 + EX31_RS11360 0.41 +0.3 203,335 - EX31_RS11360 0.41 -1.1 203,335 - EX31_RS11360 0.41 +0.6 203,335 - EX31_RS11360 0.41 -0.1 203,335 - EX31_RS11360 0.41 -0.9 203,381 + EX31_RS11360 0.46 -1.2 203,381 + EX31_RS11360 0.46 -0.1 203,382 - EX31_RS11360 0.46 +0.3 203,382 - EX31_RS11360 0.46 -1.5 203,382 - EX31_RS11360 0.46 -0.5 203,382 - EX31_RS11360 0.46 +0.4 203,382 - EX31_RS11360 0.46 +1.6 203,382 - EX31_RS11360 0.46 -0.9 203,382 - EX31_RS11360 0.46 -0.8 203,382 - EX31_RS11360 0.46 -0.5 203,504 + EX31_RS11360 0.57 -0.8 203,606 + EX31_RS11360 0.67 +0.1 203,607 - EX31_RS11360 0.67 -1.6 203,607 - EX31_RS11360 0.67 +0.4 203,607 - EX31_RS11360 0.67 -0.2 203,607 - EX31_RS11360 0.67 -0.5 203,619 - EX31_RS11360 0.68 -0.9 203,676 - EX31_RS11360 0.74 +0.4 203,676 - EX31_RS11360 0.74 +0.4 203,676 - EX31_RS11360 0.74 -0.2
Or see this region's nucleotide sequence