Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS10940 and EX31_RS10945 are separated by 99 nucleotides EX31_RS10945 and EX31_RS10950 are separated by 200 nucleotides EX31_RS10950 and EX31_RS10955 are separated by 206 nucleotides
EX31_RS10940: EX31_RS10940 - potassium channel family protein, at 116,121 to 117,044
_RS10940
EX31_RS10945: EX31_RS10945 - hypothetical protein, at 117,144 to 117,482
_RS10945
EX31_RS10950: EX31_RS10950 - NAD(P)-dependent alcohol dehydrogenase, at 117,683 to 118,741
_RS10950
EX31_RS10955: EX31_RS10955 - AI-2E family transporter, at 118,948 to 119,958
_RS10955
Position (kb)
117
118
119 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 116.752 kb on - strand, within EX31_RS10940 at 116.758 kb on + strand, within EX31_RS10940 at 116.758 kb on + strand, within EX31_RS10940 at 116.759 kb on - strand, within EX31_RS10940 at 116.759 kb on - strand, within EX31_RS10940 at 116.759 kb on - strand, within EX31_RS10940 at 116.816 kb on + strand, within EX31_RS10940 at 116.816 kb on + strand, within EX31_RS10940 at 116.819 kb on + strand, within EX31_RS10940 at 116.819 kb on + strand, within EX31_RS10940 at 116.819 kb on + strand, within EX31_RS10940 at 116.820 kb on - strand, within EX31_RS10940 at 116.820 kb on - strand, within EX31_RS10940 at 116.843 kb on - strand, within EX31_RS10940 at 116.902 kb on - strand, within EX31_RS10940 at 116.940 kb on + strand, within EX31_RS10940 at 116.950 kb on + strand, within EX31_RS10940 at 116.996 kb on + strand at 116.996 kb on + strand at 116.996 kb on + strand at 117.031 kb on + strand at 117.096 kb on - strand at 117.096 kb on - strand at 117.096 kb on - strand at 117.102 kb on + strand at 117.106 kb on + strand at 117.106 kb on + strand at 117.106 kb on + strand at 117.107 kb on - strand at 117.107 kb on - strand at 117.107 kb on - strand at 117.107 kb on - strand at 117.146 kb on - strand at 117.146 kb on - strand at 117.146 kb on - strand at 117.146 kb on - strand at 117.178 kb on + strand, within EX31_RS10945 at 117.179 kb on - strand, within EX31_RS10945 at 117.179 kb on - strand, within EX31_RS10945 at 117.179 kb on - strand, within EX31_RS10945 at 117.179 kb on - strand, within EX31_RS10945 at 117.179 kb on - strand, within EX31_RS10945 at 117.179 kb on - strand, within EX31_RS10945 at 117.179 kb on - strand, within EX31_RS10945 at 117.179 kb on - strand, within EX31_RS10945 at 117.179 kb on - strand, within EX31_RS10945 at 117.179 kb on - strand, within EX31_RS10945 at 117.179 kb on - strand, within EX31_RS10945 at 117.179 kb on - strand, within EX31_RS10945 at 117.179 kb on - strand, within EX31_RS10945 at 117.179 kb on - strand, within EX31_RS10945 at 117.179 kb on - strand, within EX31_RS10945 at 117.240 kb on - strand, within EX31_RS10945 at 117.296 kb on + strand, within EX31_RS10945 at 117.297 kb on - strand, within EX31_RS10945 at 117.314 kb on + strand, within EX31_RS10945 at 117.315 kb on - strand, within EX31_RS10945 at 117.315 kb on - strand, within EX31_RS10945 at 117.315 kb on - strand, within EX31_RS10945 at 117.364 kb on + strand, within EX31_RS10945 at 117.365 kb on - strand, within EX31_RS10945 at 117.365 kb on - strand, within EX31_RS10945 at 117.477 kb on - strand at 117.555 kb on + strand at 117.555 kb on + strand at 117.556 kb on - strand at 117.556 kb on - strand at 117.556 kb on - strand at 117.601 kb on + strand at 117.615 kb on + strand at 117.616 kb on - strand at 117.616 kb on - strand at 117.642 kb on - strand at 117.642 kb on - strand at 117.654 kb on - strand at 117.657 kb on + strand at 117.658 kb on - strand at 117.674 kb on + strand at 117.707 kb on - strand at 117.753 kb on + strand at 117.754 kb on - strand at 118.109 kb on - strand, within EX31_RS10950 at 118.245 kb on + strand, within EX31_RS10950 at 118.246 kb on - strand, within EX31_RS10950 at 118.356 kb on + strand, within EX31_RS10950 at 118.366 kb on + strand, within EX31_RS10950 at 118.366 kb on + strand, within EX31_RS10950 at 118.366 kb on + strand, within EX31_RS10950 at 118.366 kb on + strand, within EX31_RS10950 at 118.367 kb on - strand, within EX31_RS10950 at 118.367 kb on - strand, within EX31_RS10950 at 118.367 kb on - strand, within EX31_RS10950 at 118.367 kb on - strand, within EX31_RS10950 at 118.391 kb on - strand, within EX31_RS10950 at 118.392 kb on + strand, within EX31_RS10950 at 118.392 kb on + strand, within EX31_RS10950 at 118.392 kb on + strand, within EX31_RS10950 at 118.392 kb on + strand, within EX31_RS10950 at 118.392 kb on + strand, within EX31_RS10950 at 118.393 kb on - strand, within EX31_RS10950 at 118.393 kb on - strand, within EX31_RS10950 at 118.402 kb on + strand, within EX31_RS10950 at 118.419 kb on + strand, within EX31_RS10950 at 118.420 kb on - strand, within EX31_RS10950 at 118.420 kb on - strand, within EX31_RS10950 at 118.420 kb on - strand, within EX31_RS10950 at 118.471 kb on + strand, within EX31_RS10950 at 118.471 kb on + strand, within EX31_RS10950 at 118.472 kb on - strand, within EX31_RS10950 at 118.629 kb on + strand, within EX31_RS10950 at 118.746 kb on - strand at 118.768 kb on + strand at 118.806 kb on - strand at 118.815 kb on + strand at 118.816 kb on - strand at 118.831 kb on + strand at 118.831 kb on + strand at 118.831 kb on + strand at 118.832 kb on - strand at 118.832 kb on - strand at 118.832 kb on - strand at 118.832 kb on - strand at 118.833 kb on + strand at 118.834 kb on - strand at 118.849 kb on + strand at 118.849 kb on + strand at 118.850 kb on - strand at 118.868 kb on - strand at 118.950 kb on - strand at 118.991 kb on + strand at 118.991 kb on + strand at 118.992 kb on - strand at 118.992 kb on - strand at 118.995 kb on - strand at 118.995 kb on - strand at 118.995 kb on - strand at 118.995 kb on - strand at 118.995 kb on - strand at 118.995 kb on - strand at 118.999 kb on - strand at 118.999 kb on - strand at 119.115 kb on - strand, within EX31_RS10955 at 119.202 kb on + strand, within EX31_RS10955 at 119.466 kb on + strand, within EX31_RS10955 at 119.606 kb on + strand, within EX31_RS10955 at 119.607 kb on - strand, within EX31_RS10955 at 119.665 kb on - strand, within EX31_RS10955 at 119.670 kb on + strand, within EX31_RS10955
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 116,752 - EX31_RS10940 0.68 +1.4 116,758 + EX31_RS10940 0.69 +2.5 116,758 + EX31_RS10940 0.69 -0.1 116,759 - EX31_RS10940 0.69 +0.0 116,759 - EX31_RS10940 0.69 -0.9 116,759 - EX31_RS10940 0.69 +1.3 116,816 + EX31_RS10940 0.75 -1.5 116,816 + EX31_RS10940 0.75 +0.1 116,819 + EX31_RS10940 0.76 +0.4 116,819 + EX31_RS10940 0.76 +0.3 116,819 + EX31_RS10940 0.76 -0.6 116,820 - EX31_RS10940 0.76 +0.5 116,820 - EX31_RS10940 0.76 +0.6 116,843 - EX31_RS10940 0.78 -0.8 116,902 - EX31_RS10940 0.85 +0.3 116,940 + EX31_RS10940 0.89 -2.3 116,950 + EX31_RS10940 0.90 +0.6 116,996 + -0.8 116,996 + +0.2 116,996 + -0.6 117,031 + +0.3 117,096 - -0.1 117,096 - +0.4 117,096 - +1.7 117,102 + +0.8 117,106 + +0.8 117,106 + -0.4 117,106 + +0.9 117,107 - +0.2 117,107 - +1.6 117,107 - -0.5 117,107 - +0.6 117,146 - -0.1 117,146 - +0.4 117,146 - -0.7 117,146 - +0.5 117,178 + EX31_RS10945 0.10 +2.2 117,179 - EX31_RS10945 0.10 -1.0 117,179 - EX31_RS10945 0.10 +0.4 117,179 - EX31_RS10945 0.10 -1.2 117,179 - EX31_RS10945 0.10 +0.2 117,179 - EX31_RS10945 0.10 +0.1 117,179 - EX31_RS10945 0.10 +0.8 117,179 - EX31_RS10945 0.10 +0.3 117,179 - EX31_RS10945 0.10 +0.1 117,179 - EX31_RS10945 0.10 +1.9 117,179 - EX31_RS10945 0.10 +0.1 117,179 - EX31_RS10945 0.10 +0.9 117,179 - EX31_RS10945 0.10 +1.0 117,179 - EX31_RS10945 0.10 +0.8 117,179 - EX31_RS10945 0.10 +0.4 117,179 - EX31_RS10945 0.10 +3.5 117,240 - EX31_RS10945 0.28 -0.2 117,296 + EX31_RS10945 0.45 +0.6 117,297 - EX31_RS10945 0.45 +0.2 117,314 + EX31_RS10945 0.50 -0.4 117,315 - EX31_RS10945 0.50 -0.5 117,315 - EX31_RS10945 0.50 +0.8 117,315 - EX31_RS10945 0.50 +0.1 117,364 + EX31_RS10945 0.65 -0.2 117,365 - EX31_RS10945 0.65 +1.1 117,365 - EX31_RS10945 0.65 -1.0 117,477 - -1.1 117,555 + +0.1 117,555 + +1.2 117,556 - +0.8 117,556 - -0.6 117,556 - +0.2 117,601 + -1.5 117,615 + -0.6 117,616 - +1.7 117,616 - -1.2 117,642 - -2.1 117,642 - -0.9 117,654 - +0.7 117,657 + -0.2 117,658 - +0.2 117,674 + -0.0 117,707 - +0.4 117,753 + -0.2 117,754 - -1.1 118,109 - EX31_RS10950 0.40 -0.3 118,245 + EX31_RS10950 0.53 +1.2 118,246 - EX31_RS10950 0.53 -0.3 118,356 + EX31_RS10950 0.64 +0.9 118,366 + EX31_RS10950 0.64 +0.4 118,366 + EX31_RS10950 0.64 -0.4 118,366 + EX31_RS10950 0.64 -0.1 118,366 + EX31_RS10950 0.64 +2.4 118,367 - EX31_RS10950 0.65 +0.9 118,367 - EX31_RS10950 0.65 -0.6 118,367 - EX31_RS10950 0.65 -0.9 118,367 - EX31_RS10950 0.65 +0.4 118,391 - EX31_RS10950 0.67 +1.6 118,392 + EX31_RS10950 0.67 +0.6 118,392 + EX31_RS10950 0.67 +0.1 118,392 + EX31_RS10950 0.67 +1.6 118,392 + EX31_RS10950 0.67 +0.5 118,392 + EX31_RS10950 0.67 +0.4 118,393 - EX31_RS10950 0.67 +0.2 118,393 - EX31_RS10950 0.67 +0.9 118,402 + EX31_RS10950 0.68 -0.2 118,419 + EX31_RS10950 0.69 -1.1 118,420 - EX31_RS10950 0.70 +0.4 118,420 - EX31_RS10950 0.70 -0.6 118,420 - EX31_RS10950 0.70 +1.9 118,471 + EX31_RS10950 0.74 +1.2 118,471 + EX31_RS10950 0.74 -1.2 118,472 - EX31_RS10950 0.75 -0.6 118,629 + EX31_RS10950 0.89 +0.8 118,746 - -0.9 118,768 + +1.5 118,806 - -0.6 118,815 + +0.1 118,816 - -1.0 118,831 + -0.7 118,831 + +1.2 118,831 + +0.4 118,832 - +0.8 118,832 - +0.2 118,832 - +0.4 118,832 - -0.4 118,833 + -0.1 118,834 - +0.8 118,849 + -0.1 118,849 + -0.4 118,850 - -0.5 118,868 - +1.9 118,950 - +1.6 118,991 + +0.9 118,991 + +0.7 118,992 - +1.9 118,992 - +1.6 118,995 - -1.1 118,995 - +0.9 118,995 - +2.9 118,995 - +0.7 118,995 - -0.1 118,995 - +0.7 118,999 - -1.2 118,999 - +1.2 119,115 - EX31_RS10955 0.17 +1.6 119,202 + EX31_RS10955 0.25 +0.9 119,466 + EX31_RS10955 0.51 +0.4 119,606 + EX31_RS10955 0.65 +0.7 119,607 - EX31_RS10955 0.65 +0.9 119,665 - EX31_RS10955 0.71 +0.4 119,670 + EX31_RS10955 0.71 -0.8
Or see this region's nucleotide sequence