Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS10340 and EX31_RS10345 are separated by 145 nucleotides
EX31_RS10340: EX31_RS10340 - GNAT family N-acetyltransferase, at 282,060 to 282,497
_RS10340
EX31_RS10345: EX31_RS10345 - GNAT family N-acetyltransferase, at 282,643 to 283,158
_RS10345
Position (kb) 282
283
284 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 281.748 kb on + strand at 281.748 kb on + strand at 281.916 kb on + strand at 281.917 kb on - strand at 281.920 kb on + strand at 281.939 kb on + strand at 281.939 kb on + strand at 281.942 kb on + strand at 281.942 kb on + strand at 281.943 kb on - strand at 281.943 kb on - strand at 282.095 kb on - strand at 282.161 kb on + strand, within EX31_RS10340 at 282.162 kb on - strand, within EX31_RS10340 at 282.170 kb on + strand, within EX31_RS10340 at 282.226 kb on + strand, within EX31_RS10340 at 282.227 kb on - strand, within EX31_RS10340 at 282.473 kb on + strand at 282.474 kb on - strand at 282.495 kb on + strand at 282.495 kb on + strand at 282.496 kb on - strand at 282.519 kb on + strand at 282.519 kb on + strand at 282.520 kb on - strand at 282.531 kb on + strand at 282.531 kb on + strand at 282.532 kb on - strand at 282.536 kb on - strand at 282.536 kb on - strand at 282.596 kb on + strand at 282.596 kb on + strand at 282.597 kb on - strand at 282.597 kb on - strand at 282.657 kb on + strand at 282.772 kb on - strand, within EX31_RS10345 at 282.809 kb on - strand, within EX31_RS10345 at 282.809 kb on - strand, within EX31_RS10345 at 282.809 kb on - strand, within EX31_RS10345 at 282.833 kb on + strand, within EX31_RS10345 at 282.833 kb on + strand, within EX31_RS10345 at 282.834 kb on - strand, within EX31_RS10345 at 282.850 kb on + strand, within EX31_RS10345 at 282.851 kb on - strand, within EX31_RS10345 at 282.864 kb on + strand, within EX31_RS10345 at 282.865 kb on - strand, within EX31_RS10345 at 282.908 kb on - strand, within EX31_RS10345 at 282.908 kb on - strand, within EX31_RS10345 at 282.943 kb on - strand, within EX31_RS10345 at 283.034 kb on + strand, within EX31_RS10345 at 283.038 kb on + strand, within EX31_RS10345 at 283.039 kb on - strand, within EX31_RS10345 at 283.054 kb on - strand, within EX31_RS10345 at 283.212 kb on - strand at 283.405 kb on + strand at 283.421 kb on - strand at 283.421 kb on - strand at 283.441 kb on - strand at 283.451 kb on - strand at 283.505 kb on - strand at 283.523 kb on - strand at 283.528 kb on + strand at 283.528 kb on + strand at 283.528 kb on + strand at 283.529 kb on - strand at 283.529 kb on - strand at 283.530 kb on + strand at 283.530 kb on + strand at 283.530 kb on + strand at 283.530 kb on + strand at 283.530 kb on + strand at 283.531 kb on - strand at 283.531 kb on - strand at 283.531 kb on - strand at 283.531 kb on - strand at 283.531 kb on - strand at 283.531 kb on - strand at 283.531 kb on - strand at 283.531 kb on - strand at 283.531 kb on - strand at 283.531 kb on - strand at 283.531 kb on - strand at 283.584 kb on - strand at 283.609 kb on + strand at 283.609 kb on + strand at 283.624 kb on + strand at 283.624 kb on + strand at 283.625 kb on - strand at 283.625 kb on - strand at 283.625 kb on - strand at 283.628 kb on + strand at 283.681 kb on - strand at 283.698 kb on - strand at 283.728 kb on - strand at 283.828 kb on - strand at 283.887 kb on - strand at 283.889 kb on + strand at 283.952 kb on - strand at 283.963 kb on + strand at 284.065 kb on - strand at 284.066 kb on + strand at 284.066 kb on + strand at 284.066 kb on + strand at 284.068 kb on + strand at 284.068 kb on + strand at 284.069 kb on - strand at 284.081 kb on + strand at 284.090 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 281,748 + -1.8 281,748 + +0.3 281,916 + +0.2 281,917 - -1.7 281,920 + +0.5 281,939 + -1.6 281,939 + +0.3 281,942 + +0.8 281,942 + -0.1 281,943 - -1.4 281,943 - -0.8 282,095 - +0.2 282,161 + EX31_RS10340 0.23 +1.0 282,162 - EX31_RS10340 0.23 -0.2 282,170 + EX31_RS10340 0.25 +1.8 282,226 + EX31_RS10340 0.38 +0.8 282,227 - EX31_RS10340 0.38 -0.9 282,473 + -0.1 282,474 - -0.2 282,495 + +2.2 282,495 + -0.4 282,496 - +0.6 282,519 + +1.2 282,519 + +1.2 282,520 - +0.5 282,531 + +2.0 282,531 + +0.8 282,532 - -0.6 282,536 - +0.6 282,536 - -1.2 282,596 + -0.7 282,596 + -0.9 282,597 - +1.0 282,597 - -1.8 282,657 + -0.4 282,772 - EX31_RS10345 0.25 -1.6 282,809 - EX31_RS10345 0.32 +0.8 282,809 - EX31_RS10345 0.32 +1.2 282,809 - EX31_RS10345 0.32 -0.7 282,833 + EX31_RS10345 0.37 -0.4 282,833 + EX31_RS10345 0.37 -1.0 282,834 - EX31_RS10345 0.37 +0.0 282,850 + EX31_RS10345 0.40 -2.0 282,851 - EX31_RS10345 0.40 +0.8 282,864 + EX31_RS10345 0.43 -1.0 282,865 - EX31_RS10345 0.43 +1.8 282,908 - EX31_RS10345 0.51 -0.4 282,908 - EX31_RS10345 0.51 -1.6 282,943 - EX31_RS10345 0.58 +0.4 283,034 + EX31_RS10345 0.76 -0.6 283,038 + EX31_RS10345 0.77 +0.1 283,039 - EX31_RS10345 0.77 +0.4 283,054 - EX31_RS10345 0.80 +0.6 283,212 - +0.8 283,405 + +0.4 283,421 - +0.8 283,421 - +0.2 283,441 - +0.2 283,451 - -0.6 283,505 - -1.4 283,523 - +0.8 283,528 + +2.1 283,528 + -0.2 283,528 + -1.0 283,529 - -1.3 283,529 - +0.8 283,530 + -1.2 283,530 + -0.4 283,530 + -0.2 283,530 + +0.5 283,530 + +0.8 283,531 - +0.7 283,531 - +1.4 283,531 - +0.8 283,531 - -0.5 283,531 - +1.2 283,531 - +1.4 283,531 - +1.8 283,531 - +0.4 283,531 - +1.4 283,531 - +1.2 283,531 - +0.1 283,584 - +0.1 283,609 + +1.6 283,609 + -0.2 283,624 + +1.0 283,624 + +0.3 283,625 - +0.2 283,625 - +0.7 283,625 - +0.6 283,628 + -2.0 283,681 - -0.4 283,698 - -0.5 283,728 - +3.5 283,828 - +1.6 283,887 - +0.2 283,889 + +0.1 283,952 - +0.5 283,963 + -1.1 284,065 - +0.3 284,066 + +0.2 284,066 + -0.4 284,066 + +0.2 284,068 + -0.4 284,068 + +0.6 284,069 - -0.6 284,081 + +1.5 284,090 - -1.7
Or see this region's nucleotide sequence