Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09930 and EX31_RS09935 are separated by 25 nucleotides EX31_RS09935 and EX31_RS09940 are separated by 148 nucleotides EX31_RS09940 and EX31_RS09945 are separated by 198 nucleotides EX31_RS09945 and EX31_RS09950 are separated by 34 nucleotides
EX31_RS09930: EX31_RS09930 - adenosylcobinamide-GDP ribazoletransferase, at 194,916 to 195,668
_RS09930
EX31_RS09935: EX31_RS09935 - bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase, at 195,694 to 196,248
_RS09935
EX31_RS09940: EX31_RS09940 - 23S rRNA pseudouridine(2605) synthase RluB, at 196,397 to 197,716
_RS09940
EX31_RS09945: EX31_RS09945 - threonylcarbamoyl-AMP synthase, at 197,915 to 198,535
_RS09945
EX31_RS09950: EX31_RS09950 - PHP domain-containing protein, at 198,570 to 199,493
_RS09950
Position (kb) 196
197
198 Strain fitness (log2 ratio) -2
-1
0
1
2 at 195.427 kb on - strand, within EX31_RS09930 at 195.427 kb on - strand, within EX31_RS09930 at 195.427 kb on - strand, within EX31_RS09930 at 195.444 kb on - strand, within EX31_RS09930 at 195.446 kb on + strand, within EX31_RS09930 at 195.446 kb on + strand, within EX31_RS09930 at 195.447 kb on - strand, within EX31_RS09930 at 195.447 kb on - strand, within EX31_RS09930 at 195.447 kb on - strand, within EX31_RS09930 at 195.447 kb on - strand, within EX31_RS09930 at 195.574 kb on + strand, within EX31_RS09930 at 195.574 kb on + strand, within EX31_RS09930 at 195.574 kb on + strand, within EX31_RS09930 at 195.575 kb on - strand, within EX31_RS09930 at 195.575 kb on - strand, within EX31_RS09930 at 195.575 kb on - strand, within EX31_RS09930 at 195.661 kb on - strand at 195.671 kb on + strand at 195.672 kb on - strand at 195.672 kb on - strand at 195.695 kb on + strand at 195.696 kb on - strand at 195.741 kb on - strand at 195.741 kb on - strand at 195.741 kb on - strand at 195.790 kb on - strand, within EX31_RS09935 at 195.926 kb on - strand, within EX31_RS09935 at 196.014 kb on + strand, within EX31_RS09935 at 196.080 kb on + strand, within EX31_RS09935 at 196.081 kb on - strand, within EX31_RS09935 at 196.143 kb on + strand, within EX31_RS09935 at 196.143 kb on + strand, within EX31_RS09935 at 196.144 kb on - strand, within EX31_RS09935 at 196.148 kb on - strand, within EX31_RS09935 at 196.163 kb on + strand, within EX31_RS09935 at 196.163 kb on + strand, within EX31_RS09935 at 196.164 kb on - strand, within EX31_RS09935 at 196.164 kb on - strand, within EX31_RS09935 at 196.164 kb on - strand, within EX31_RS09935 at 196.164 kb on - strand, within EX31_RS09935 at 196.164 kb on - strand, within EX31_RS09935 at 196.216 kb on - strand at 196.240 kb on + strand at 196.249 kb on - strand at 196.267 kb on + strand at 196.267 kb on + strand at 196.270 kb on + strand at 196.280 kb on + strand at 196.280 kb on + strand at 196.312 kb on + strand at 196.369 kb on + strand at 196.370 kb on - strand at 196.448 kb on - strand at 196.484 kb on + strand at 196.558 kb on - strand, within EX31_RS09940 at 196.558 kb on - strand, within EX31_RS09940 at 196.558 kb on - strand, within EX31_RS09940 at 196.558 kb on - strand, within EX31_RS09940 at 196.586 kb on - strand, within EX31_RS09940 at 196.588 kb on - strand, within EX31_RS09940 at 196.723 kb on - strand, within EX31_RS09940 at 196.723 kb on - strand, within EX31_RS09940 at 196.723 kb on - strand, within EX31_RS09940 at 196.847 kb on + strand, within EX31_RS09940 at 196.848 kb on - strand, within EX31_RS09940 at 196.855 kb on - strand, within EX31_RS09940 at 196.920 kb on - strand, within EX31_RS09940 at 196.956 kb on - strand, within EX31_RS09940 at 197.056 kb on - strand, within EX31_RS09940 at 197.056 kb on - strand, within EX31_RS09940 at 197.056 kb on - strand, within EX31_RS09940 at 197.056 kb on - strand, within EX31_RS09940 at 197.056 kb on - strand, within EX31_RS09940 at 197.056 kb on - strand, within EX31_RS09940 at 197.056 kb on - strand, within EX31_RS09940 at 197.056 kb on - strand, within EX31_RS09940 at 197.056 kb on - strand, within EX31_RS09940 at 197.059 kb on - strand, within EX31_RS09940 at 197.059 kb on - strand, within EX31_RS09940 at 197.059 kb on - strand, within EX31_RS09940 at 197.059 kb on - strand, within EX31_RS09940 at 197.059 kb on - strand, within EX31_RS09940 at 197.059 kb on - strand, within EX31_RS09940 at 197.311 kb on - strand, within EX31_RS09940 at 197.360 kb on - strand, within EX31_RS09940 at 197.360 kb on - strand, within EX31_RS09940 at 197.360 kb on - strand, within EX31_RS09940 at 197.449 kb on + strand, within EX31_RS09940 at 197.449 kb on + strand, within EX31_RS09940 at 197.450 kb on - strand, within EX31_RS09940 at 197.564 kb on - strand, within EX31_RS09940 at 197.702 kb on - strand at 197.702 kb on - strand at 197.703 kb on + strand at 197.704 kb on - strand at 197.815 kb on + strand at 197.848 kb on - strand at 197.857 kb on + strand at 197.857 kb on + strand at 197.858 kb on - strand at 197.858 kb on - strand at 197.858 kb on - strand at 197.858 kb on - strand at 197.858 kb on - strand at 197.858 kb on - strand at 197.858 kb on - strand at 197.860 kb on - strand at 198.188 kb on - strand, within EX31_RS09945 at 198.246 kb on + strand, within EX31_RS09945 at 198.247 kb on - strand, within EX31_RS09945 at 198.298 kb on - strand, within EX31_RS09945 at 198.298 kb on - strand, within EX31_RS09945 at 198.298 kb on - strand, within EX31_RS09945 at 198.303 kb on + strand, within EX31_RS09945 at 198.303 kb on + strand, within EX31_RS09945 at 198.303 kb on + strand, within EX31_RS09945 at 198.304 kb on - strand, within EX31_RS09945 at 198.304 kb on - strand, within EX31_RS09945 at 198.347 kb on - strand, within EX31_RS09945 at 198.347 kb on - strand, within EX31_RS09945 at 198.379 kb on + strand, within EX31_RS09945 at 198.379 kb on + strand, within EX31_RS09945 at 198.380 kb on - strand, within EX31_RS09945 at 198.380 kb on - strand, within EX31_RS09945 at 198.484 kb on + strand at 198.517 kb on + strand at 198.518 kb on - strand at 198.518 kb on - strand at 198.526 kb on - strand at 198.546 kb on + strand at 198.547 kb on - strand at 198.631 kb on - strand at 198.631 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 195,427 - EX31_RS09930 0.68 +1.2 195,427 - EX31_RS09930 0.68 -1.5 195,427 - EX31_RS09930 0.68 +1.2 195,444 - EX31_RS09930 0.70 +0.5 195,446 + EX31_RS09930 0.70 -1.5 195,446 + EX31_RS09930 0.70 -2.1 195,447 - EX31_RS09930 0.71 +0.8 195,447 - EX31_RS09930 0.71 -0.1 195,447 - EX31_RS09930 0.71 +1.6 195,447 - EX31_RS09930 0.71 -0.9 195,574 + EX31_RS09930 0.87 +1.1 195,574 + EX31_RS09930 0.87 -0.2 195,574 + EX31_RS09930 0.87 -1.0 195,575 - EX31_RS09930 0.88 -0.7 195,575 - EX31_RS09930 0.88 -0.1 195,575 - EX31_RS09930 0.88 -0.2 195,661 - +1.1 195,671 + -0.1 195,672 - -1.8 195,672 - -0.6 195,695 + -0.4 195,696 - +1.7 195,741 - +0.1 195,741 - +0.7 195,741 - -0.3 195,790 - EX31_RS09935 0.17 -0.5 195,926 - EX31_RS09935 0.42 -0.1 196,014 + EX31_RS09935 0.58 +1.2 196,080 + EX31_RS09935 0.70 +0.4 196,081 - EX31_RS09935 0.70 -0.8 196,143 + EX31_RS09935 0.81 -1.1 196,143 + EX31_RS09935 0.81 +0.0 196,144 - EX31_RS09935 0.81 +1.0 196,148 - EX31_RS09935 0.82 +1.4 196,163 + EX31_RS09935 0.85 +0.2 196,163 + EX31_RS09935 0.85 +0.5 196,164 - EX31_RS09935 0.85 +1.1 196,164 - EX31_RS09935 0.85 +0.1 196,164 - EX31_RS09935 0.85 -2.1 196,164 - EX31_RS09935 0.85 -0.5 196,164 - EX31_RS09935 0.85 +0.8 196,216 - -1.5 196,240 + -0.7 196,249 - +0.7 196,267 + -0.1 196,267 + +0.9 196,270 + -2.2 196,280 + +1.1 196,280 + +1.3 196,312 + -0.8 196,369 + -1.1 196,370 - -0.5 196,448 - +0.3 196,484 + +0.2 196,558 - EX31_RS09940 0.12 -0.9 196,558 - EX31_RS09940 0.12 -0.8 196,558 - EX31_RS09940 0.12 -1.9 196,558 - EX31_RS09940 0.12 -1.6 196,586 - EX31_RS09940 0.14 +1.5 196,588 - EX31_RS09940 0.14 +0.2 196,723 - EX31_RS09940 0.25 -1.7 196,723 - EX31_RS09940 0.25 -0.8 196,723 - EX31_RS09940 0.25 -1.5 196,847 + EX31_RS09940 0.34 -1.9 196,848 - EX31_RS09940 0.34 -1.2 196,855 - EX31_RS09940 0.35 -1.4 196,920 - EX31_RS09940 0.40 +0.8 196,956 - EX31_RS09940 0.42 +0.2 197,056 - EX31_RS09940 0.50 -0.8 197,056 - EX31_RS09940 0.50 -1.1 197,056 - EX31_RS09940 0.50 -0.5 197,056 - EX31_RS09940 0.50 -0.5 197,056 - EX31_RS09940 0.50 +1.8 197,056 - EX31_RS09940 0.50 -1.2 197,056 - EX31_RS09940 0.50 +0.1 197,056 - EX31_RS09940 0.50 -0.5 197,056 - EX31_RS09940 0.50 -0.5 197,059 - EX31_RS09940 0.50 -0.1 197,059 - EX31_RS09940 0.50 -0.7 197,059 - EX31_RS09940 0.50 -1.5 197,059 - EX31_RS09940 0.50 -1.3 197,059 - EX31_RS09940 0.50 -0.8 197,059 - EX31_RS09940 0.50 -0.2 197,311 - EX31_RS09940 0.69 -0.4 197,360 - EX31_RS09940 0.73 -0.4 197,360 - EX31_RS09940 0.73 +0.1 197,360 - EX31_RS09940 0.73 +0.2 197,449 + EX31_RS09940 0.80 +1.2 197,449 + EX31_RS09940 0.80 +0.2 197,450 - EX31_RS09940 0.80 -1.9 197,564 - EX31_RS09940 0.88 -1.5 197,702 - -1.4 197,702 - -2.1 197,703 + -0.2 197,704 - -0.8 197,815 + +0.2 197,848 - -0.8 197,857 + -1.4 197,857 + -0.2 197,858 - +0.1 197,858 - +0.1 197,858 - -0.6 197,858 - -0.4 197,858 - -0.4 197,858 - -0.2 197,858 - +0.2 197,860 - -0.8 198,188 - EX31_RS09945 0.44 +1.6 198,246 + EX31_RS09945 0.53 +0.4 198,247 - EX31_RS09945 0.53 +1.0 198,298 - EX31_RS09945 0.62 -1.2 198,298 - EX31_RS09945 0.62 +0.6 198,298 - EX31_RS09945 0.62 -1.0 198,303 + EX31_RS09945 0.62 -0.6 198,303 + EX31_RS09945 0.62 +1.1 198,303 + EX31_RS09945 0.62 +0.0 198,304 - EX31_RS09945 0.63 +1.8 198,304 - EX31_RS09945 0.63 -0.6 198,347 - EX31_RS09945 0.70 +0.8 198,347 - EX31_RS09945 0.70 -0.2 198,379 + EX31_RS09945 0.75 +0.2 198,379 + EX31_RS09945 0.75 +0.2 198,380 - EX31_RS09945 0.75 -0.2 198,380 - EX31_RS09945 0.75 -0.5 198,484 + +0.5 198,517 + +0.4 198,518 - +1.1 198,518 - -2.6 198,526 - +0.7 198,546 + -1.5 198,547 - -0.0 198,631 - +1.1 198,631 - -0.8
Or see this region's nucleotide sequence