Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09890 and EX31_RS09895 are separated by 350 nucleotides EX31_RS09895 and EX31_RS09900 are separated by 463 nucleotides EX31_RS09900 and EX31_RS09905 are separated by 114 nucleotides
EX31_RS09890: EX31_RS09890 - HTH-type transcriptional regulator CysB, at 185,691 to 186,665
_RS09890
EX31_RS09895: EX31_RS09895 - type I DNA topoisomerase, at 187,016 to 189,613
_RS09895
EX31_RS09900: EX31_RS09900 - YciN family protein, at 190,077 to 190,328
_RS09900
EX31_RS09905: EX31_RS09905 - protease SohB, at 190,443 to 191,486
_RS09905
Position (kb) 187
188
189
190 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 186.074 kb on + strand, within EX31_RS09890 at 186.074 kb on + strand, within EX31_RS09890 at 186.075 kb on - strand, within EX31_RS09890 at 186.075 kb on - strand, within EX31_RS09890 at 186.075 kb on - strand, within EX31_RS09890 at 186.077 kb on - strand, within EX31_RS09890 at 186.077 kb on - strand, within EX31_RS09890 at 186.077 kb on - strand, within EX31_RS09890 at 186.126 kb on - strand, within EX31_RS09890 at 186.176 kb on - strand, within EX31_RS09890 at 186.203 kb on - strand, within EX31_RS09890 at 186.291 kb on - strand, within EX31_RS09890 at 186.330 kb on - strand, within EX31_RS09890 at 186.363 kb on - strand, within EX31_RS09890 at 186.363 kb on - strand, within EX31_RS09890 at 186.363 kb on - strand, within EX31_RS09890 at 186.380 kb on - strand, within EX31_RS09890 at 186.380 kb on - strand, within EX31_RS09890 at 186.400 kb on + strand, within EX31_RS09890 at 186.405 kb on - strand, within EX31_RS09890 at 186.405 kb on - strand, within EX31_RS09890 at 186.405 kb on - strand, within EX31_RS09890 at 186.405 kb on - strand, within EX31_RS09890 at 186.405 kb on - strand, within EX31_RS09890 at 186.405 kb on - strand, within EX31_RS09890 at 186.405 kb on - strand, within EX31_RS09890 at 186.441 kb on - strand, within EX31_RS09890 at 186.448 kb on - strand, within EX31_RS09890 at 186.448 kb on - strand, within EX31_RS09890 at 186.448 kb on - strand, within EX31_RS09890 at 186.448 kb on - strand, within EX31_RS09890 at 186.448 kb on - strand, within EX31_RS09890 at 186.587 kb on - strand at 186.587 kb on - strand at 186.587 kb on - strand at 186.627 kb on - strand at 186.666 kb on - strand at 186.672 kb on + strand at 186.672 kb on + strand at 186.673 kb on - strand at 186.673 kb on - strand at 186.673 kb on - strand at 186.673 kb on - strand at 186.673 kb on - strand at 186.860 kb on + strand at 186.860 kb on + strand at 186.861 kb on - strand at 186.861 kb on - strand at 186.861 kb on - strand at 186.875 kb on + strand at 186.876 kb on - strand at 186.920 kb on + strand at 186.921 kb on - strand at 186.921 kb on - strand at 186.934 kb on + strand at 186.935 kb on - strand at 186.942 kb on - strand at 187.003 kb on - strand at 187.003 kb on - strand at 187.003 kb on - strand at 187.003 kb on - strand at 187.018 kb on - strand at 187.040 kb on - strand at 187.040 kb on - strand at 187.040 kb on - strand at 187.040 kb on - strand at 187.095 kb on - strand at 187.095 kb on - strand at 187.095 kb on - strand at 187.095 kb on - strand at 187.095 kb on - strand at 187.095 kb on - strand at 187.099 kb on - strand at 187.099 kb on - strand at 187.099 kb on - strand at 187.099 kb on - strand at 187.099 kb on - strand at 187.099 kb on - strand at 187.099 kb on - strand at 187.139 kb on - strand at 187.139 kb on - strand at 187.309 kb on - strand, within EX31_RS09895 at 187.432 kb on - strand, within EX31_RS09895 at 187.432 kb on - strand, within EX31_RS09895 at 187.432 kb on - strand, within EX31_RS09895 at 187.432 kb on - strand, within EX31_RS09895 at 187.432 kb on - strand, within EX31_RS09895 at 187.436 kb on - strand, within EX31_RS09895 at 187.436 kb on - strand, within EX31_RS09895 at 187.504 kb on - strand, within EX31_RS09895 at 189.667 kb on - strand at 189.697 kb on - strand at 189.699 kb on - strand at 189.792 kb on - strand at 189.836 kb on - strand at 189.889 kb on + strand at 189.889 kb on + strand at 189.890 kb on - strand at 189.890 kb on - strand at 189.890 kb on - strand at 189.915 kb on + strand at 189.916 kb on - strand at 189.916 kb on - strand at 189.916 kb on - strand at 189.978 kb on - strand at 190.045 kb on - strand at 190.045 kb on - strand at 190.057 kb on + strand at 190.057 kb on + strand at 190.057 kb on + strand at 190.057 kb on + strand at 190.057 kb on + strand at 190.057 kb on + strand at 190.058 kb on - strand at 190.058 kb on - strand at 190.058 kb on - strand at 190.069 kb on + strand at 190.073 kb on + strand at 190.073 kb on + strand at 190.073 kb on + strand at 190.088 kb on + strand at 190.088 kb on + strand at 190.100 kb on + strand at 190.155 kb on + strand, within EX31_RS09900 at 190.155 kb on + strand, within EX31_RS09900 at 190.155 kb on + strand, within EX31_RS09900 at 190.155 kb on + strand, within EX31_RS09900 at 190.156 kb on - strand, within EX31_RS09900 at 190.156 kb on - strand, within EX31_RS09900 at 190.271 kb on + strand, within EX31_RS09900 at 190.271 kb on + strand, within EX31_RS09900 at 190.271 kb on + strand, within EX31_RS09900 at 190.272 kb on - strand, within EX31_RS09900 at 190.272 kb on - strand, within EX31_RS09900 at 190.274 kb on + strand, within EX31_RS09900 at 190.274 kb on + strand, within EX31_RS09900 at 190.274 kb on + strand, within EX31_RS09900 at 190.274 kb on + strand, within EX31_RS09900 at 190.274 kb on + strand, within EX31_RS09900 at 190.274 kb on + strand, within EX31_RS09900 at 190.275 kb on - strand, within EX31_RS09900 at 190.343 kb on + strand at 190.343 kb on + strand at 190.346 kb on + strand at 190.346 kb on + strand at 190.346 kb on + strand at 190.346 kb on + strand at 190.347 kb on - strand at 190.353 kb on - strand at 190.354 kb on + strand at 190.354 kb on + strand at 190.354 kb on + strand at 190.354 kb on + strand at 190.354 kb on + strand at 190.354 kb on + strand at 190.354 kb on + strand at 190.354 kb on + strand at 190.355 kb on - strand at 190.355 kb on - strand at 190.379 kb on + strand at 190.393 kb on - strand at 190.421 kb on + strand at 190.422 kb on - strand at 190.444 kb on + strand at 190.444 kb on + strand at 190.444 kb on + strand at 190.471 kb on - strand at 190.471 kb on - strand at 190.563 kb on + strand, within EX31_RS09905 at 190.564 kb on - strand, within EX31_RS09905
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 186,074 + EX31_RS09890 0.39 -0.5 186,074 + EX31_RS09890 0.39 +0.2 186,075 - EX31_RS09890 0.39 -1.4 186,075 - EX31_RS09890 0.39 -2.1 186,075 - EX31_RS09890 0.39 -2.6 186,077 - EX31_RS09890 0.40 -2.0 186,077 - EX31_RS09890 0.40 -1.4 186,077 - EX31_RS09890 0.40 -1.4 186,126 - EX31_RS09890 0.45 -3.3 186,176 - EX31_RS09890 0.50 +0.2 186,203 - EX31_RS09890 0.53 -2.6 186,291 - EX31_RS09890 0.62 -1.5 186,330 - EX31_RS09890 0.66 -2.4 186,363 - EX31_RS09890 0.69 -0.5 186,363 - EX31_RS09890 0.69 +0.8 186,363 - EX31_RS09890 0.69 -2.6 186,380 - EX31_RS09890 0.71 -2.5 186,380 - EX31_RS09890 0.71 -3.6 186,400 + EX31_RS09890 0.73 +1.8 186,405 - EX31_RS09890 0.73 -0.5 186,405 - EX31_RS09890 0.73 -0.8 186,405 - EX31_RS09890 0.73 -3.1 186,405 - EX31_RS09890 0.73 -0.5 186,405 - EX31_RS09890 0.73 -3.4 186,405 - EX31_RS09890 0.73 -2.8 186,405 - EX31_RS09890 0.73 -0.7 186,441 - EX31_RS09890 0.77 -3.0 186,448 - EX31_RS09890 0.78 -1.5 186,448 - EX31_RS09890 0.78 -0.4 186,448 - EX31_RS09890 0.78 -0.8 186,448 - EX31_RS09890 0.78 -2.6 186,448 - EX31_RS09890 0.78 -0.7 186,587 - -0.5 186,587 - -3.2 186,587 - -0.7 186,627 - -2.7 186,666 - -1.5 186,672 + -1.2 186,672 + -0.2 186,673 - -1.5 186,673 - -0.8 186,673 - -4.6 186,673 - -1.5 186,673 - -1.0 186,860 + +0.1 186,860 + +1.1 186,861 - -0.2 186,861 - -0.1 186,861 - +0.4 186,875 + -0.9 186,876 - +0.3 186,920 + +0.2 186,921 - -1.8 186,921 - -1.0 186,934 + +0.2 186,935 - -1.1 186,942 - +0.6 187,003 - +0.2 187,003 - +0.1 187,003 - -0.6 187,003 - +1.1 187,018 - -0.4 187,040 - -1.4 187,040 - +0.1 187,040 - -0.4 187,040 - -1.2 187,095 - +0.1 187,095 - -0.8 187,095 - -0.6 187,095 - -0.4 187,095 - -0.1 187,095 - -0.8 187,099 - -1.6 187,099 - +0.8 187,099 - -0.8 187,099 - -1.0 187,099 - -0.6 187,099 - -0.1 187,099 - -0.0 187,139 - -0.5 187,139 - -1.0 187,309 - EX31_RS09895 0.11 -1.9 187,432 - EX31_RS09895 0.16 -1.1 187,432 - EX31_RS09895 0.16 -0.8 187,432 - EX31_RS09895 0.16 -2.6 187,432 - EX31_RS09895 0.16 -0.8 187,432 - EX31_RS09895 0.16 +0.2 187,436 - EX31_RS09895 0.16 +0.2 187,436 - EX31_RS09895 0.16 +0.2 187,504 - EX31_RS09895 0.19 +0.2 189,667 - +1.8 189,697 - -0.8 189,699 - -0.3 189,792 - +0.2 189,836 - +0.6 189,889 + +1.4 189,889 + -0.8 189,890 - +1.0 189,890 - -1.0 189,890 - +0.4 189,915 + +0.8 189,916 - +2.4 189,916 - +1.2 189,916 - -0.1 189,978 - +0.6 190,045 - -0.4 190,045 - -0.4 190,057 + -0.3 190,057 + +0.8 190,057 + -0.3 190,057 + -0.9 190,057 + +0.2 190,057 + -0.1 190,058 - +1.5 190,058 - +2.1 190,058 - +1.2 190,069 + +0.4 190,073 + -0.9 190,073 + -0.1 190,073 + +0.5 190,088 + -2.0 190,088 + +0.8 190,100 + +0.6 190,155 + EX31_RS09900 0.31 -1.0 190,155 + EX31_RS09900 0.31 +0.1 190,155 + EX31_RS09900 0.31 -0.8 190,155 + EX31_RS09900 0.31 -0.2 190,156 - EX31_RS09900 0.31 -0.5 190,156 - EX31_RS09900 0.31 -1.4 190,271 + EX31_RS09900 0.77 +0.8 190,271 + EX31_RS09900 0.77 +0.1 190,271 + EX31_RS09900 0.77 +0.0 190,272 - EX31_RS09900 0.77 -0.4 190,272 - EX31_RS09900 0.77 -0.0 190,274 + EX31_RS09900 0.78 +0.0 190,274 + EX31_RS09900 0.78 +0.8 190,274 + EX31_RS09900 0.78 -1.1 190,274 + EX31_RS09900 0.78 +0.9 190,274 + EX31_RS09900 0.78 +0.3 190,274 + EX31_RS09900 0.78 +0.5 190,275 - EX31_RS09900 0.79 +0.0 190,343 + +0.2 190,343 + -0.7 190,346 + +0.1 190,346 + -2.3 190,346 + -1.1 190,346 + -0.4 190,347 - +0.1 190,353 - +1.8 190,354 + -0.6 190,354 + +2.2 190,354 + -0.4 190,354 + -1.6 190,354 + +0.8 190,354 + -0.2 190,354 + -0.8 190,354 + +1.8 190,355 - -0.9 190,355 - -0.8 190,379 + -0.4 190,393 - -0.2 190,421 + -1.4 190,422 - -0.8 190,444 + +0.7 190,444 + +0.1 190,444 + +0.8 190,471 - +0.2 190,471 - +0.4 190,563 + EX31_RS09905 0.11 +1.2 190,564 - EX31_RS09905 0.12 -1.0
Or see this region's nucleotide sequence