Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09825 and EX31_RS09830 are separated by 5 nucleotides EX31_RS09830 and EX31_RS09835 are separated by 60 nucleotides EX31_RS09835 and EX31_RS09840 are separated by 42 nucleotides
EX31_RS09825: EX31_RS09825 - glycyl-radical enzyme activating protein, at 172,116 to 173,015
_RS09825
EX31_RS09830: EX31_RS09830 - formate C-acetyltransferase/glycerol dehydratase family glycyl radical enzyme, at 173,021 to 175,453
_RS09830
EX31_RS09835: EX31_RS09835 - DeoR/GlpR transcriptional regulator, at 175,514 to 176,257
_RS09835
EX31_RS09840: EX31_RS09840 - L-ribulose-5-phosphate 4-epimerase, at 176,300 to 176,995
_RS09840
Position (kb) 173
174
175
176 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 172.137 kb on + strand at 172.265 kb on - strand, within EX31_RS09825 at 172.318 kb on + strand, within EX31_RS09825 at 172.318 kb on + strand, within EX31_RS09825 at 172.319 kb on - strand, within EX31_RS09825 at 172.319 kb on - strand, within EX31_RS09825 at 172.473 kb on + strand, within EX31_RS09825 at 172.473 kb on + strand, within EX31_RS09825 at 172.615 kb on - strand, within EX31_RS09825 at 172.724 kb on + strand, within EX31_RS09825 at 172.896 kb on + strand, within EX31_RS09825 at 172.896 kb on + strand, within EX31_RS09825 at 172.896 kb on + strand, within EX31_RS09825 at 172.896 kb on + strand, within EX31_RS09825 at 172.896 kb on + strand, within EX31_RS09825 at 172.897 kb on - strand, within EX31_RS09825 at 172.897 kb on - strand, within EX31_RS09825 at 172.918 kb on - strand, within EX31_RS09825 at 172.918 kb on - strand, within EX31_RS09825 at 172.918 kb on - strand, within EX31_RS09825 at 172.918 kb on - strand, within EX31_RS09825 at 172.974 kb on + strand at 172.974 kb on + strand at 172.975 kb on - strand at 173.005 kb on + strand at 173.005 kb on + strand at 173.005 kb on + strand at 173.069 kb on - strand at 173.104 kb on - strand at 173.126 kb on + strand at 173.126 kb on + strand at 173.127 kb on - strand at 173.212 kb on + strand at 173.213 kb on - strand at 173.288 kb on + strand, within EX31_RS09830 at 173.429 kb on + strand, within EX31_RS09830 at 173.494 kb on + strand, within EX31_RS09830 at 173.495 kb on - strand, within EX31_RS09830 at 173.495 kb on - strand, within EX31_RS09830 at 173.495 kb on - strand, within EX31_RS09830 at 173.825 kb on + strand, within EX31_RS09830 at 173.895 kb on - strand, within EX31_RS09830 at 173.909 kb on + strand, within EX31_RS09830 at 174.051 kb on - strand, within EX31_RS09830 at 174.051 kb on - strand, within EX31_RS09830 at 174.051 kb on - strand, within EX31_RS09830 at 174.122 kb on + strand, within EX31_RS09830 at 174.260 kb on - strand, within EX31_RS09830 at 174.320 kb on + strand, within EX31_RS09830 at 174.321 kb on - strand, within EX31_RS09830 at 174.326 kb on + strand, within EX31_RS09830 at 174.326 kb on + strand, within EX31_RS09830 at 174.551 kb on + strand, within EX31_RS09830 at 174.551 kb on + strand, within EX31_RS09830 at 174.551 kb on + strand, within EX31_RS09830 at 174.551 kb on + strand, within EX31_RS09830 at 174.551 kb on + strand, within EX31_RS09830 at 174.552 kb on - strand, within EX31_RS09830 at 174.552 kb on - strand, within EX31_RS09830 at 174.552 kb on - strand, within EX31_RS09830 at 174.552 kb on - strand, within EX31_RS09830 at 174.616 kb on + strand, within EX31_RS09830 at 174.646 kb on + strand, within EX31_RS09830 at 174.646 kb on + strand, within EX31_RS09830 at 174.646 kb on + strand, within EX31_RS09830 at 174.648 kb on + strand, within EX31_RS09830 at 174.648 kb on + strand, within EX31_RS09830 at 174.648 kb on + strand, within EX31_RS09830 at 174.649 kb on - strand, within EX31_RS09830 at 174.649 kb on - strand, within EX31_RS09830 at 174.686 kb on + strand, within EX31_RS09830 at 174.686 kb on + strand, within EX31_RS09830 at 174.815 kb on + strand, within EX31_RS09830 at 174.872 kb on + strand, within EX31_RS09830 at 174.898 kb on + strand, within EX31_RS09830 at 174.898 kb on + strand, within EX31_RS09830 at 174.926 kb on + strand, within EX31_RS09830 at 174.926 kb on + strand, within EX31_RS09830 at 174.926 kb on + strand, within EX31_RS09830 at 174.927 kb on - strand, within EX31_RS09830 at 174.927 kb on - strand, within EX31_RS09830 at 174.989 kb on + strand, within EX31_RS09830 at 174.989 kb on + strand, within EX31_RS09830 at 174.989 kb on + strand, within EX31_RS09830 at 174.989 kb on + strand, within EX31_RS09830 at 174.990 kb on - strand, within EX31_RS09830 at 174.993 kb on - strand, within EX31_RS09830 at 175.063 kb on + strand, within EX31_RS09830 at 175.072 kb on + strand, within EX31_RS09830 at 175.138 kb on + strand, within EX31_RS09830 at 175.243 kb on - strand at 175.300 kb on + strand at 175.320 kb on + strand at 175.321 kb on - strand at 175.324 kb on + strand at 175.324 kb on + strand at 175.325 kb on - strand at 175.350 kb on - strand at 175.423 kb on + strand at 175.423 kb on + strand at 175.423 kb on + strand at 175.424 kb on - strand at 175.424 kb on - strand at 175.424 kb on - strand at 175.426 kb on + strand at 175.452 kb on - strand at 175.500 kb on + strand at 175.513 kb on + strand at 175.534 kb on + strand at 175.627 kb on + strand, within EX31_RS09835 at 175.649 kb on + strand, within EX31_RS09835 at 175.682 kb on - strand, within EX31_RS09835 at 175.824 kb on - strand, within EX31_RS09835 at 175.868 kb on + strand, within EX31_RS09835 at 175.869 kb on - strand, within EX31_RS09835 at 175.891 kb on + strand, within EX31_RS09835 at 175.891 kb on + strand, within EX31_RS09835 at 176.055 kb on + strand, within EX31_RS09835 at 176.055 kb on + strand, within EX31_RS09835 at 176.055 kb on + strand, within EX31_RS09835 at 176.056 kb on - strand, within EX31_RS09835 at 176.056 kb on - strand, within EX31_RS09835 at 176.207 kb on + strand at 176.237 kb on - strand at 176.260 kb on + strand at 176.260 kb on + strand at 176.261 kb on - strand at 176.267 kb on - strand at 176.301 kb on + strand at 176.302 kb on - strand at 176.314 kb on - strand at 176.347 kb on + strand at 176.347 kb on + strand at 176.348 kb on - strand at 176.413 kb on - strand, within EX31_RS09840
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 172,137 + +0.8 172,265 - EX31_RS09825 0.17 -0.2 172,318 + EX31_RS09825 0.22 +1.0 172,318 + EX31_RS09825 0.22 +0.3 172,319 - EX31_RS09825 0.23 +0.4 172,319 - EX31_RS09825 0.23 -1.2 172,473 + EX31_RS09825 0.40 +1.1 172,473 + EX31_RS09825 0.40 +0.8 172,615 - EX31_RS09825 0.55 +0.2 172,724 + EX31_RS09825 0.68 +0.5 172,896 + EX31_RS09825 0.87 +0.7 172,896 + EX31_RS09825 0.87 -0.1 172,896 + EX31_RS09825 0.87 -1.3 172,896 + EX31_RS09825 0.87 +0.8 172,896 + EX31_RS09825 0.87 +0.8 172,897 - EX31_RS09825 0.87 +0.2 172,897 - EX31_RS09825 0.87 -0.4 172,918 - EX31_RS09825 0.89 +1.0 172,918 - EX31_RS09825 0.89 -0.5 172,918 - EX31_RS09825 0.89 +0.0 172,918 - EX31_RS09825 0.89 -0.2 172,974 + +1.7 172,974 + -0.2 172,975 - -0.2 173,005 + +1.5 173,005 + -1.3 173,005 + +1.6 173,069 - -0.4 173,104 - -0.2 173,126 + -1.4 173,126 + -0.8 173,127 - -0.5 173,212 + +0.5 173,213 - +1.8 173,288 + EX31_RS09830 0.11 +0.2 173,429 + EX31_RS09830 0.17 +0.2 173,494 + EX31_RS09830 0.19 +0.8 173,495 - EX31_RS09830 0.19 -0.9 173,495 - EX31_RS09830 0.19 -0.3 173,495 - EX31_RS09830 0.19 -0.7 173,825 + EX31_RS09830 0.33 +1.8 173,895 - EX31_RS09830 0.36 -1.6 173,909 + EX31_RS09830 0.36 -0.3 174,051 - EX31_RS09830 0.42 -1.9 174,051 - EX31_RS09830 0.42 +1.5 174,051 - EX31_RS09830 0.42 +1.2 174,122 + EX31_RS09830 0.45 +0.2 174,260 - EX31_RS09830 0.51 +0.4 174,320 + EX31_RS09830 0.53 -0.4 174,321 - EX31_RS09830 0.53 -1.6 174,326 + EX31_RS09830 0.54 +0.1 174,326 + EX31_RS09830 0.54 +0.8 174,551 + EX31_RS09830 0.63 -1.1 174,551 + EX31_RS09830 0.63 -0.0 174,551 + EX31_RS09830 0.63 -0.8 174,551 + EX31_RS09830 0.63 -0.1 174,551 + EX31_RS09830 0.63 +1.6 174,552 - EX31_RS09830 0.63 +1.3 174,552 - EX31_RS09830 0.63 +1.2 174,552 - EX31_RS09830 0.63 -1.3 174,552 - EX31_RS09830 0.63 +0.2 174,616 + EX31_RS09830 0.66 -1.7 174,646 + EX31_RS09830 0.67 +0.0 174,646 + EX31_RS09830 0.67 -0.1 174,646 + EX31_RS09830 0.67 +0.5 174,648 + EX31_RS09830 0.67 +1.1 174,648 + EX31_RS09830 0.67 +0.8 174,648 + EX31_RS09830 0.67 +0.8 174,649 - EX31_RS09830 0.67 +1.4 174,649 - EX31_RS09830 0.67 -1.6 174,686 + EX31_RS09830 0.68 +1.1 174,686 + EX31_RS09830 0.68 +2.8 174,815 + EX31_RS09830 0.74 -1.6 174,872 + EX31_RS09830 0.76 -0.2 174,898 + EX31_RS09830 0.77 +0.5 174,898 + EX31_RS09830 0.77 -0.2 174,926 + EX31_RS09830 0.78 +2.8 174,926 + EX31_RS09830 0.78 -0.9 174,926 + EX31_RS09830 0.78 -0.9 174,927 - EX31_RS09830 0.78 +0.5 174,927 - EX31_RS09830 0.78 -1.0 174,989 + EX31_RS09830 0.81 +1.2 174,989 + EX31_RS09830 0.81 +0.5 174,989 + EX31_RS09830 0.81 +0.8 174,989 + EX31_RS09830 0.81 +0.7 174,990 - EX31_RS09830 0.81 +0.6 174,993 - EX31_RS09830 0.81 -0.3 175,063 + EX31_RS09830 0.84 -1.5 175,072 + EX31_RS09830 0.84 -0.2 175,138 + EX31_RS09830 0.87 +0.6 175,243 - +2.4 175,300 + -0.2 175,320 + -0.1 175,321 - +0.4 175,324 + -0.6 175,324 + +0.2 175,325 - -1.2 175,350 - +3.0 175,423 + -1.2 175,423 + -1.4 175,423 + +1.8 175,424 - +0.4 175,424 - -0.8 175,424 - -0.6 175,426 + +0.5 175,452 - +0.1 175,500 + +1.0 175,513 + +1.5 175,534 + +2.7 175,627 + EX31_RS09835 0.15 +0.3 175,649 + EX31_RS09835 0.18 +0.5 175,682 - EX31_RS09835 0.23 -0.1 175,824 - EX31_RS09835 0.42 +0.5 175,868 + EX31_RS09835 0.48 +1.0 175,869 - EX31_RS09835 0.48 +2.8 175,891 + EX31_RS09835 0.51 +1.8 175,891 + EX31_RS09835 0.51 -0.5 176,055 + EX31_RS09835 0.73 -1.2 176,055 + EX31_RS09835 0.73 -0.1 176,055 + EX31_RS09835 0.73 -1.2 176,056 - EX31_RS09835 0.73 +1.8 176,056 - EX31_RS09835 0.73 +0.0 176,207 + -2.1 176,237 - +0.4 176,260 + +0.5 176,260 + +3.1 176,261 - -1.5 176,267 - +2.4 176,301 + -0.8 176,302 - -1.6 176,314 - +1.0 176,347 + -0.2 176,347 + +1.8 176,348 - +2.6 176,413 - EX31_RS09840 0.16 +0.8
Or see this region's nucleotide sequence