Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09820 and EX31_RS09825 are separated by 147 nucleotides EX31_RS09825 and EX31_RS09830 are separated by 5 nucleotides
EX31_RS09820: EX31_RS09820 - fructose-6-phosphate aldolase, at 171,303 to 171,968
_RS09820
EX31_RS09825: EX31_RS09825 - glycyl-radical enzyme activating protein, at 172,116 to 173,015
_RS09825
EX31_RS09830: EX31_RS09830 - formate C-acetyltransferase/glycerol dehydratase family glycyl radical enzyme, at 173,021 to 175,453
_RS09830
Position (kb) 172
173
174 Strain fitness (log2 ratio) -1
0
1
2 at 171.187 kb on + strand at 171.222 kb on - strand at 171.345 kb on - strand at 171.381 kb on - strand, within EX31_RS09820 at 171.381 kb on - strand, within EX31_RS09820 at 171.381 kb on - strand, within EX31_RS09820 at 171.388 kb on - strand, within EX31_RS09820 at 171.388 kb on - strand, within EX31_RS09820 at 171.577 kb on + strand, within EX31_RS09820 at 171.589 kb on + strand, within EX31_RS09820 at 171.589 kb on + strand, within EX31_RS09820 at 171.590 kb on - strand, within EX31_RS09820 at 171.660 kb on - strand, within EX31_RS09820 at 171.894 kb on - strand, within EX31_RS09820 at 171.931 kb on - strand at 171.958 kb on + strand at 172.015 kb on + strand at 172.015 kb on + strand at 172.016 kb on - strand at 172.016 kb on - strand at 172.016 kb on - strand at 172.137 kb on + strand at 172.265 kb on - strand, within EX31_RS09825 at 172.318 kb on + strand, within EX31_RS09825 at 172.318 kb on + strand, within EX31_RS09825 at 172.319 kb on - strand, within EX31_RS09825 at 172.319 kb on - strand, within EX31_RS09825 at 172.473 kb on + strand, within EX31_RS09825 at 172.473 kb on + strand, within EX31_RS09825 at 172.615 kb on - strand, within EX31_RS09825 at 172.724 kb on + strand, within EX31_RS09825 at 172.896 kb on + strand, within EX31_RS09825 at 172.896 kb on + strand, within EX31_RS09825 at 172.896 kb on + strand, within EX31_RS09825 at 172.896 kb on + strand, within EX31_RS09825 at 172.896 kb on + strand, within EX31_RS09825 at 172.897 kb on - strand, within EX31_RS09825 at 172.897 kb on - strand, within EX31_RS09825 at 172.918 kb on - strand, within EX31_RS09825 at 172.918 kb on - strand, within EX31_RS09825 at 172.918 kb on - strand, within EX31_RS09825 at 172.918 kb on - strand, within EX31_RS09825 at 172.974 kb on + strand at 172.974 kb on + strand at 172.975 kb on - strand at 173.005 kb on + strand at 173.005 kb on + strand at 173.005 kb on + strand at 173.069 kb on - strand at 173.104 kb on - strand at 173.126 kb on + strand at 173.126 kb on + strand at 173.127 kb on - strand at 173.212 kb on + strand at 173.213 kb on - strand at 173.288 kb on + strand, within EX31_RS09830 at 173.429 kb on + strand, within EX31_RS09830 at 173.494 kb on + strand, within EX31_RS09830 at 173.495 kb on - strand, within EX31_RS09830 at 173.495 kb on - strand, within EX31_RS09830 at 173.495 kb on - strand, within EX31_RS09830 at 173.825 kb on + strand, within EX31_RS09830 at 173.895 kb on - strand, within EX31_RS09830 at 173.909 kb on + strand, within EX31_RS09830
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 171,187 + +2.0 171,222 - +0.9 171,345 - +0.2 171,381 - EX31_RS09820 0.12 +1.2 171,381 - EX31_RS09820 0.12 -1.2 171,381 - EX31_RS09820 0.12 -1.0 171,388 - EX31_RS09820 0.13 +1.2 171,388 - EX31_RS09820 0.13 -0.7 171,577 + EX31_RS09820 0.41 +1.6 171,589 + EX31_RS09820 0.43 +1.2 171,589 + EX31_RS09820 0.43 +2.4 171,590 - EX31_RS09820 0.43 -1.0 171,660 - EX31_RS09820 0.54 +1.1 171,894 - EX31_RS09820 0.89 +0.5 171,931 - -0.8 171,958 + +1.1 172,015 + -1.0 172,015 + -0.2 172,016 - -1.6 172,016 - -0.1 172,016 - -0.2 172,137 + +0.8 172,265 - EX31_RS09825 0.17 -0.2 172,318 + EX31_RS09825 0.22 +1.0 172,318 + EX31_RS09825 0.22 +0.3 172,319 - EX31_RS09825 0.23 +0.4 172,319 - EX31_RS09825 0.23 -1.2 172,473 + EX31_RS09825 0.40 +1.1 172,473 + EX31_RS09825 0.40 +0.8 172,615 - EX31_RS09825 0.55 +0.2 172,724 + EX31_RS09825 0.68 +0.5 172,896 + EX31_RS09825 0.87 +0.7 172,896 + EX31_RS09825 0.87 -0.1 172,896 + EX31_RS09825 0.87 -1.3 172,896 + EX31_RS09825 0.87 +0.8 172,896 + EX31_RS09825 0.87 +0.8 172,897 - EX31_RS09825 0.87 +0.2 172,897 - EX31_RS09825 0.87 -0.4 172,918 - EX31_RS09825 0.89 +1.0 172,918 - EX31_RS09825 0.89 -0.5 172,918 - EX31_RS09825 0.89 +0.0 172,918 - EX31_RS09825 0.89 -0.2 172,974 + +1.7 172,974 + -0.2 172,975 - -0.2 173,005 + +1.5 173,005 + -1.3 173,005 + +1.6 173,069 - -0.4 173,104 - -0.2 173,126 + -1.4 173,126 + -0.8 173,127 - -0.5 173,212 + +0.5 173,213 - +1.8 173,288 + EX31_RS09830 0.11 +0.2 173,429 + EX31_RS09830 0.17 +0.2 173,494 + EX31_RS09830 0.19 +0.8 173,495 - EX31_RS09830 0.19 -0.9 173,495 - EX31_RS09830 0.19 -0.3 173,495 - EX31_RS09830 0.19 -0.7 173,825 + EX31_RS09830 0.33 +1.8 173,895 - EX31_RS09830 0.36 -1.6 173,909 + EX31_RS09830 0.36 -0.3
Or see this region's nucleotide sequence