Strain Fitness in Rahnella sp. WP5 around EX31_RS06820

Experiment: L-Glutamine (N); ShewMM media; no shaking

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS06815 and EX31_RS06820 are separated by 18 nucleotidesEX31_RS06820 and EX31_RS06825 are separated by 13 nucleotidesEX31_RS06825 and EX31_RS06830 are separated by 38 nucleotides EX31_RS06815: EX31_RS06815 - argininosuccinate lyase, at 150,616 to 152,043 _RS06815 EX31_RS06820: EX31_RS06820 - amino acid ABC transporter permease, at 152,062 to 152,934 _RS06820 EX31_RS06825: EX31_RS06825 - amino acid ABC transporter ATP-binding protein, at 152,948 to 153,712 _RS06825 EX31_RS06830: EX31_RS06830 - ABC transporter substrate-binding protein, at 153,751 to 154,572 _RS06830 Position (kb) 152 153Strain fitness (log2 ratio) -1 0 1 2at 151.065 kb on + strand, within EX31_RS06815at 151.066 kb on - strand, within EX31_RS06815at 151.071 kb on + strand, within EX31_RS06815at 151.072 kb on - strand, within EX31_RS06815at 151.090 kb on - strand, within EX31_RS06815at 151.576 kb on + strand, within EX31_RS06815at 151.605 kb on + strand, within EX31_RS06815at 151.605 kb on + strand, within EX31_RS06815at 151.606 kb on - strand, within EX31_RS06815at 151.606 kb on - strand, within EX31_RS06815at 151.637 kb on + strand, within EX31_RS06815at 151.638 kb on - strand, within EX31_RS06815at 151.668 kb on + strand, within EX31_RS06815at 151.719 kb on + strand, within EX31_RS06815at 151.719 kb on + strand, within EX31_RS06815at 151.720 kb on - strand, within EX31_RS06815at 151.821 kb on + strand, within EX31_RS06815at 151.821 kb on + strand, within EX31_RS06815at 151.821 kb on + strand, within EX31_RS06815at 151.822 kb on - strand, within EX31_RS06815at 151.865 kb on + strand, within EX31_RS06815at 151.865 kb on + strand, within EX31_RS06815at 152.017 kb on - strandat 152.018 kb on + strandat 152.018 kb on + strandat 152.018 kb on + strandat 152.019 kb on - strandat 152.024 kb on - strandat 152.039 kb on + strandat 152.128 kb on + strandat 152.128 kb on + strandat 152.143 kb on + strandat 152.147 kb on - strandat 152.233 kb on + strand, within EX31_RS06820at 152.392 kb on + strand, within EX31_RS06820at 152.394 kb on + strand, within EX31_RS06820at 152.394 kb on + strand, within EX31_RS06820at 152.394 kb on + strand, within EX31_RS06820at 152.394 kb on + strand, within EX31_RS06820at 152.563 kb on + strand, within EX31_RS06820at 152.563 kb on + strand, within EX31_RS06820at 152.776 kb on + strand, within EX31_RS06820at 152.776 kb on + strand, within EX31_RS06820at 152.777 kb on - strand, within EX31_RS06820at 152.794 kb on + strand, within EX31_RS06820at 152.794 kb on + strand, within EX31_RS06820at 152.795 kb on - strand, within EX31_RS06820at 152.896 kb on + strandat 152.896 kb on + strandat 152.959 kb on + strandat 152.980 kb on + strandat 152.984 kb on + strandat 152.984 kb on + strandat 152.985 kb on - strandat 152.985 kb on - strandat 153.231 kb on + strand, within EX31_RS06825at 153.231 kb on + strand, within EX31_RS06825at 153.232 kb on - strand, within EX31_RS06825at 153.370 kb on + strand, within EX31_RS06825at 153.573 kb on + strand, within EX31_RS06825at 153.574 kb on - strand, within EX31_RS06825at 153.630 kb on + strand, within EX31_RS06825at 153.631 kb on - strand, within EX31_RS06825at 153.652 kb on + strandat 153.849 kb on + strand, within EX31_RS06830

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking
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151,065 + EX31_RS06815 0.31 +0.2
151,066 - EX31_RS06815 0.32 +0.8
151,071 + EX31_RS06815 0.32 +0.3
151,072 - EX31_RS06815 0.32 +1.8
151,090 - EX31_RS06815 0.33 -1.1
151,576 + EX31_RS06815 0.67 +0.8
151,605 + EX31_RS06815 0.69 -0.6
151,605 + EX31_RS06815 0.69 +0.2
151,606 - EX31_RS06815 0.69 +1.8
151,606 - EX31_RS06815 0.69 -0.1
151,637 + EX31_RS06815 0.71 -1.7
151,638 - EX31_RS06815 0.72 -1.1
151,668 + EX31_RS06815 0.74 +0.1
151,719 + EX31_RS06815 0.77 -0.1
151,719 + EX31_RS06815 0.77 +1.8
151,720 - EX31_RS06815 0.77 +1.0
151,821 + EX31_RS06815 0.84 -1.1
151,821 + EX31_RS06815 0.84 +1.1
151,821 + EX31_RS06815 0.84 +0.2
151,822 - EX31_RS06815 0.84 +0.9
151,865 + EX31_RS06815 0.87 -0.2
151,865 + EX31_RS06815 0.87 +0.6
152,017 - +1.8
152,018 + +0.3
152,018 + -0.4
152,018 + +1.3
152,019 - -0.6
152,024 - +0.3
152,039 + +0.6
152,128 + -1.1
152,128 + +0.2
152,143 + +2.2
152,147 - +0.5
152,233 + EX31_RS06820 0.20 +2.5
152,392 + EX31_RS06820 0.38 +1.3
152,394 + EX31_RS06820 0.38 +1.8
152,394 + EX31_RS06820 0.38 -0.8
152,394 + EX31_RS06820 0.38 +0.5
152,394 + EX31_RS06820 0.38 -1.1
152,563 + EX31_RS06820 0.57 -0.2
152,563 + EX31_RS06820 0.57 +2.0
152,776 + EX31_RS06820 0.82 +2.1
152,776 + EX31_RS06820 0.82 +0.8
152,777 - EX31_RS06820 0.82 -0.7
152,794 + EX31_RS06820 0.84 -0.3
152,794 + EX31_RS06820 0.84 +0.6
152,795 - EX31_RS06820 0.84 +0.8
152,896 + -0.1
152,896 + +0.5
152,959 + -0.2
152,980 + +0.1
152,984 + -0.2
152,984 + +0.2
152,985 - -0.6
152,985 - -0.9
153,231 + EX31_RS06825 0.37 -0.2
153,231 + EX31_RS06825 0.37 +0.2
153,232 - EX31_RS06825 0.37 +0.9
153,370 + EX31_RS06825 0.55 -1.6
153,573 + EX31_RS06825 0.82 +0.8
153,574 - EX31_RS06825 0.82 +1.8
153,630 + EX31_RS06825 0.89 -0.6
153,631 - EX31_RS06825 0.89 +1.3
153,652 + -0.1
153,849 + EX31_RS06830 0.12 -0.2

Or see this region's nucleotide sequence