Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS00885 and EX31_RS00895 are separated by 523 nucleotides EX31_RS00895 and EX31_RS25045 are separated by 141 nucleotides EX31_RS25045 and EX31_RS00900 are separated by 304 nucleotides
EX31_RS00885: EX31_RS00885 - transcriptional regulator CecR, at 192,848 to 193,519
_RS00885
EX31_RS00895: EX31_RS00895 - iron-containing alcohol dehydrogenase, at 194,043 to 195,203
_RS00895
EX31_RS25045: EX31_RS25045 - IS5 family transposase, at 195,345 to 195,782
_RS25045
EX31_RS00900: EX31_RS00900 - mechanosensitive ion channel, at 196,087 to 197,709
_RS00900
Position (kb) 194
195
196 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 193.051 kb on - strand, within EX31_RS00885 at 193.091 kb on + strand, within EX31_RS00885 at 193.220 kb on + strand, within EX31_RS00885 at 193.261 kb on + strand, within EX31_RS00885 at 193.262 kb on - strand, within EX31_RS00885 at 193.338 kb on + strand, within EX31_RS00885 at 193.338 kb on + strand, within EX31_RS00885 at 193.338 kb on + strand, within EX31_RS00885 at 193.338 kb on + strand, within EX31_RS00885 at 193.338 kb on + strand, within EX31_RS00885 at 193.338 kb on + strand, within EX31_RS00885 at 193.338 kb on + strand, within EX31_RS00885 at 193.339 kb on - strand, within EX31_RS00885 at 193.339 kb on - strand, within EX31_RS00885 at 193.339 kb on - strand, within EX31_RS00885 at 193.339 kb on - strand, within EX31_RS00885 at 193.339 kb on - strand, within EX31_RS00885 at 193.366 kb on - strand, within EX31_RS00885 at 193.406 kb on - strand, within EX31_RS00885 at 193.459 kb on + strand at 193.460 kb on - strand at 193.539 kb on - strand at 193.574 kb on + strand at 193.575 kb on - strand at 193.645 kb on + strand at 193.645 kb on + strand at 193.646 kb on - strand at 193.650 kb on - strand at 193.650 kb on - strand at 193.650 kb on - strand at 193.708 kb on + strand at 193.860 kb on - strand at 193.904 kb on - strand at 193.926 kb on + strand at 193.983 kb on + strand at 194.011 kb on + strand at 194.012 kb on - strand at 194.024 kb on + strand at 194.025 kb on - strand at 194.052 kb on + strand at 194.053 kb on - strand at 194.053 kb on - strand at 194.055 kb on + strand at 194.056 kb on - strand at 194.056 kb on - strand at 194.077 kb on + strand at 194.109 kb on - strand at 194.125 kb on + strand at 194.126 kb on - strand at 194.131 kb on + strand at 194.195 kb on + strand, within EX31_RS00895 at 194.230 kb on + strand, within EX31_RS00895 at 194.230 kb on + strand, within EX31_RS00895 at 194.231 kb on - strand, within EX31_RS00895 at 194.231 kb on - strand, within EX31_RS00895 at 194.231 kb on - strand, within EX31_RS00895 at 194.249 kb on + strand, within EX31_RS00895 at 194.357 kb on + strand, within EX31_RS00895 at 194.405 kb on + strand, within EX31_RS00895 at 194.411 kb on + strand, within EX31_RS00895 at 194.426 kb on + strand, within EX31_RS00895 at 194.426 kb on + strand, within EX31_RS00895 at 194.572 kb on + strand, within EX31_RS00895 at 194.647 kb on - strand, within EX31_RS00895 at 194.647 kb on - strand, within EX31_RS00895 at 194.647 kb on - strand, within EX31_RS00895 at 194.647 kb on - strand, within EX31_RS00895 at 194.654 kb on + strand, within EX31_RS00895 at 194.715 kb on + strand, within EX31_RS00895 at 194.715 kb on + strand, within EX31_RS00895 at 194.716 kb on - strand, within EX31_RS00895 at 194.745 kb on + strand, within EX31_RS00895 at 194.745 kb on + strand, within EX31_RS00895 at 194.745 kb on + strand, within EX31_RS00895 at 194.747 kb on + strand, within EX31_RS00895 at 194.747 kb on + strand, within EX31_RS00895 at 194.747 kb on + strand, within EX31_RS00895 at 194.747 kb on + strand, within EX31_RS00895 at 194.747 kb on + strand, within EX31_RS00895 at 194.748 kb on - strand, within EX31_RS00895 at 194.748 kb on - strand, within EX31_RS00895 at 194.748 kb on - strand, within EX31_RS00895 at 194.748 kb on - strand, within EX31_RS00895 at 194.748 kb on - strand, within EX31_RS00895 at 194.765 kb on + strand, within EX31_RS00895 at 194.766 kb on - strand, within EX31_RS00895 at 194.801 kb on + strand, within EX31_RS00895 at 194.820 kb on + strand, within EX31_RS00895 at 194.820 kb on + strand, within EX31_RS00895 at 194.821 kb on - strand, within EX31_RS00895 at 194.856 kb on + strand, within EX31_RS00895 at 194.856 kb on + strand, within EX31_RS00895 at 194.856 kb on + strand, within EX31_RS00895 at 194.857 kb on - strand, within EX31_RS00895 at 194.857 kb on - strand, within EX31_RS00895 at 194.857 kb on - strand, within EX31_RS00895 at 194.857 kb on - strand, within EX31_RS00895 at 194.857 kb on - strand, within EX31_RS00895 at 194.858 kb on + strand, within EX31_RS00895 at 194.875 kb on + strand, within EX31_RS00895 at 194.876 kb on - strand, within EX31_RS00895 at 194.879 kb on + strand, within EX31_RS00895 at 194.879 kb on + strand, within EX31_RS00895 at 194.879 kb on + strand, within EX31_RS00895 at 194.879 kb on + strand, within EX31_RS00895 at 194.879 kb on + strand, within EX31_RS00895 at 194.879 kb on + strand, within EX31_RS00895 at 194.879 kb on + strand, within EX31_RS00895 at 194.879 kb on + strand, within EX31_RS00895 at 194.880 kb on - strand, within EX31_RS00895 at 194.880 kb on - strand, within EX31_RS00895 at 194.880 kb on - strand, within EX31_RS00895 at 194.880 kb on - strand, within EX31_RS00895 at 194.880 kb on - strand, within EX31_RS00895 at 194.880 kb on - strand, within EX31_RS00895 at 194.880 kb on - strand, within EX31_RS00895 at 194.880 kb on - strand, within EX31_RS00895 at 194.880 kb on - strand, within EX31_RS00895 at 194.898 kb on + strand, within EX31_RS00895 at 194.898 kb on + strand, within EX31_RS00895 at 194.898 kb on + strand, within EX31_RS00895 at 194.899 kb on - strand, within EX31_RS00895 at 194.899 kb on - strand, within EX31_RS00895 at 194.912 kb on + strand, within EX31_RS00895 at 194.912 kb on + strand, within EX31_RS00895 at 194.931 kb on - strand, within EX31_RS00895 at 194.984 kb on + strand, within EX31_RS00895 at 195.174 kb on + strand at 195.207 kb on + strand at 195.211 kb on + strand at 195.211 kb on + strand at 195.211 kb on + strand at 195.211 kb on + strand at 195.212 kb on - strand at 195.212 kb on - strand at 195.247 kb on + strand at 195.256 kb on + strand at 195.298 kb on - strand at 195.312 kb on + strand at 195.312 kb on + strand at 195.313 kb on - strand at 195.313 kb on - strand at 195.313 kb on - strand at 195.313 kb on - strand at 195.315 kb on + strand at 195.316 kb on - strand at 195.316 kb on - strand at 195.316 kb on - strand at 195.316 kb on - strand at 195.316 kb on - strand at 195.316 kb on - strand at 195.316 kb on - strand at 195.316 kb on - strand at 195.316 kb on - strand at 195.319 kb on + strand at 195.320 kb on - strand at 195.336 kb on + strand at 195.336 kb on + strand at 195.337 kb on - strand at 195.337 kb on - strand at 195.337 kb on - strand at 195.339 kb on + strand at 195.339 kb on + strand at 195.340 kb on - strand at 195.340 kb on - strand at 195.340 kb on - strand at 195.340 kb on - strand at 195.345 kb on - strand at 195.414 kb on - strand, within EX31_RS25045 at 195.414 kb on - strand, within EX31_RS25045 at 195.505 kb on + strand, within EX31_RS25045 at 195.506 kb on - strand, within EX31_RS25045 at 195.506 kb on - strand, within EX31_RS25045 at 195.510 kb on - strand, within EX31_RS25045 at 195.689 kb on + strand, within EX31_RS25045 at 195.689 kb on + strand, within EX31_RS25045 at 195.690 kb on - strand, within EX31_RS25045 at 195.691 kb on + strand, within EX31_RS25045 at 195.691 kb on + strand, within EX31_RS25045 at 195.701 kb on + strand, within EX31_RS25045 at 195.702 kb on - strand, within EX31_RS25045 at 195.820 kb on + strand at 195.821 kb on - strand at 195.821 kb on - strand at 195.823 kb on + strand at 195.824 kb on - strand at 195.835 kb on - strand at 195.863 kb on + strand at 195.878 kb on + strand at 195.878 kb on + strand at 195.879 kb on - strand at 195.932 kb on + strand at 195.935 kb on + strand at 195.935 kb on + strand at 195.936 kb on - strand at 196.017 kb on - strand at 196.031 kb on + strand at 196.031 kb on + strand at 196.032 kb on - strand at 196.033 kb on + strand at 196.033 kb on + strand at 196.034 kb on - strand at 196.085 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 193,051 - EX31_RS00885 0.30 +0.1 193,091 + EX31_RS00885 0.36 +1.3 193,220 + EX31_RS00885 0.55 -2.1 193,261 + EX31_RS00885 0.61 +0.4 193,262 - EX31_RS00885 0.62 -0.3 193,338 + EX31_RS00885 0.73 -0.1 193,338 + EX31_RS00885 0.73 -0.2 193,338 + EX31_RS00885 0.73 +0.7 193,338 + EX31_RS00885 0.73 -0.5 193,338 + EX31_RS00885 0.73 +0.5 193,338 + EX31_RS00885 0.73 +0.5 193,338 + EX31_RS00885 0.73 +0.4 193,339 - EX31_RS00885 0.73 -0.8 193,339 - EX31_RS00885 0.73 +1.5 193,339 - EX31_RS00885 0.73 -0.0 193,339 - EX31_RS00885 0.73 -0.9 193,339 - EX31_RS00885 0.73 -0.4 193,366 - EX31_RS00885 0.77 -0.7 193,406 - EX31_RS00885 0.83 +1.1 193,459 + +0.7 193,460 - +0.1 193,539 - -0.5 193,574 + +0.2 193,575 - +0.7 193,645 + +1.9 193,645 + -0.4 193,646 - +0.2 193,650 - +2.1 193,650 - +2.3 193,650 - -0.3 193,708 + -0.9 193,860 - +1.8 193,904 - -0.5 193,926 + +0.3 193,983 + -0.4 194,011 + +0.3 194,012 - +0.6 194,024 + -1.1 194,025 - +0.6 194,052 + +1.6 194,053 - +0.8 194,053 - -0.0 194,055 + +0.5 194,056 - +0.3 194,056 - +0.3 194,077 + +1.4 194,109 - +0.4 194,125 + +0.7 194,126 - -0.5 194,131 + -2.3 194,195 + EX31_RS00895 0.13 +0.7 194,230 + EX31_RS00895 0.16 -0.6 194,230 + EX31_RS00895 0.16 -1.6 194,231 - EX31_RS00895 0.16 +0.1 194,231 - EX31_RS00895 0.16 -0.8 194,231 - EX31_RS00895 0.16 +0.5 194,249 + EX31_RS00895 0.18 +0.1 194,357 + EX31_RS00895 0.27 +1.3 194,405 + EX31_RS00895 0.31 +0.6 194,411 + EX31_RS00895 0.32 +0.7 194,426 + EX31_RS00895 0.33 -0.4 194,426 + EX31_RS00895 0.33 +0.4 194,572 + EX31_RS00895 0.46 -0.4 194,647 - EX31_RS00895 0.52 +0.2 194,647 - EX31_RS00895 0.52 +1.7 194,647 - EX31_RS00895 0.52 -0.1 194,647 - EX31_RS00895 0.52 +1.1 194,654 + EX31_RS00895 0.53 +0.8 194,715 + EX31_RS00895 0.58 -0.9 194,715 + EX31_RS00895 0.58 -0.9 194,716 - EX31_RS00895 0.58 +0.0 194,745 + EX31_RS00895 0.60 +0.1 194,745 + EX31_RS00895 0.60 +0.7 194,745 + EX31_RS00895 0.60 +0.7 194,747 + EX31_RS00895 0.61 +0.8 194,747 + EX31_RS00895 0.61 +0.1 194,747 + EX31_RS00895 0.61 -0.4 194,747 + EX31_RS00895 0.61 -1.3 194,747 + EX31_RS00895 0.61 +0.5 194,748 - EX31_RS00895 0.61 -0.0 194,748 - EX31_RS00895 0.61 +1.5 194,748 - EX31_RS00895 0.61 +1.7 194,748 - EX31_RS00895 0.61 +0.4 194,748 - EX31_RS00895 0.61 +0.3 194,765 + EX31_RS00895 0.62 -0.6 194,766 - EX31_RS00895 0.62 +0.9 194,801 + EX31_RS00895 0.65 -0.9 194,820 + EX31_RS00895 0.67 -1.3 194,820 + EX31_RS00895 0.67 -0.4 194,821 - EX31_RS00895 0.67 -1.4 194,856 + EX31_RS00895 0.70 -1.2 194,856 + EX31_RS00895 0.70 -1.0 194,856 + EX31_RS00895 0.70 -1.5 194,857 - EX31_RS00895 0.70 +0.3 194,857 - EX31_RS00895 0.70 +1.0 194,857 - EX31_RS00895 0.70 +1.2 194,857 - EX31_RS00895 0.70 -0.7 194,857 - EX31_RS00895 0.70 +0.3 194,858 + EX31_RS00895 0.70 -1.1 194,875 + EX31_RS00895 0.72 -0.6 194,876 - EX31_RS00895 0.72 +0.2 194,879 + EX31_RS00895 0.72 -0.3 194,879 + EX31_RS00895 0.72 +2.2 194,879 + EX31_RS00895 0.72 +1.0 194,879 + EX31_RS00895 0.72 +1.1 194,879 + EX31_RS00895 0.72 +3.1 194,879 + EX31_RS00895 0.72 -0.1 194,879 + EX31_RS00895 0.72 -0.3 194,879 + EX31_RS00895 0.72 +1.1 194,880 - EX31_RS00895 0.72 -0.1 194,880 - EX31_RS00895 0.72 -0.2 194,880 - EX31_RS00895 0.72 +0.3 194,880 - EX31_RS00895 0.72 +1.1 194,880 - EX31_RS00895 0.72 +1.5 194,880 - EX31_RS00895 0.72 -0.1 194,880 - EX31_RS00895 0.72 +0.7 194,880 - EX31_RS00895 0.72 +0.5 194,880 - EX31_RS00895 0.72 -1.9 194,898 + EX31_RS00895 0.74 +1.6 194,898 + EX31_RS00895 0.74 +3.4 194,898 + EX31_RS00895 0.74 +0.3 194,899 - EX31_RS00895 0.74 -0.1 194,899 - EX31_RS00895 0.74 -0.7 194,912 + EX31_RS00895 0.75 -0.0 194,912 + EX31_RS00895 0.75 +0.8 194,931 - EX31_RS00895 0.76 -1.3 194,984 + EX31_RS00895 0.81 +1.3 195,174 + -0.1 195,207 + -1.6 195,211 + -0.6 195,211 + +0.5 195,211 + +0.1 195,211 + -0.7 195,212 - +0.7 195,212 - +1.3 195,247 + +0.5 195,256 + -0.5 195,298 - -2.1 195,312 + -0.0 195,312 + -1.1 195,313 - +0.1 195,313 - -0.7 195,313 - -0.3 195,313 - -0.5 195,315 + -0.7 195,316 - +0.7 195,316 - -0.9 195,316 - +1.1 195,316 - -0.8 195,316 - -0.4 195,316 - +0.2 195,316 - +2.3 195,316 - -0.9 195,316 - -0.5 195,319 + -0.8 195,320 - -1.8 195,336 + +0.8 195,336 + +0.5 195,337 - -0.0 195,337 - +0.9 195,337 - +0.7 195,339 + -0.9 195,339 + +3.1 195,340 - +0.5 195,340 - +2.1 195,340 - +0.7 195,340 - +0.8 195,345 - +0.1 195,414 - EX31_RS25045 0.16 +0.0 195,414 - EX31_RS25045 0.16 +2.1 195,505 + EX31_RS25045 0.37 -0.5 195,506 - EX31_RS25045 0.37 +0.6 195,506 - EX31_RS25045 0.37 -0.0 195,510 - EX31_RS25045 0.38 +0.0 195,689 + EX31_RS25045 0.79 +0.3 195,689 + EX31_RS25045 0.79 +1.4 195,690 - EX31_RS25045 0.79 +0.7 195,691 + EX31_RS25045 0.79 +0.7 195,691 + EX31_RS25045 0.79 -0.3 195,701 + EX31_RS25045 0.81 -1.6 195,702 - EX31_RS25045 0.82 +2.3 195,820 + -0.2 195,821 - +0.3 195,821 - -0.3 195,823 + -0.3 195,824 - +0.7 195,835 - -0.9 195,863 + -0.1 195,878 + +0.1 195,878 + -0.6 195,879 - -1.2 195,932 + +0.8 195,935 + -0.3 195,935 + +0.0 195,936 - -1.5 196,017 - -0.3 196,031 + -0.4 196,031 + +2.1 196,032 - +0.3 196,033 + +1.1 196,033 + +3.1 196,034 - -0.9 196,085 - +1.1
Or see this region's nucleotide sequence