Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS00570 and EX31_RS00575 are separated by 39 nucleotides EX31_RS00575 and EX31_RS00580 are separated by 87 nucleotides EX31_RS00580 and EX31_RS00585 are separated by 115 nucleotides EX31_RS00585 and EX31_RS00590 are separated by 60 nucleotides EX31_RS00590 and EX31_RS00595 overlap by 4 nucleotides
EX31_RS00570: EX31_RS00570 - UbiX family flavin prenyltransferase, at 122,129 to 122,698
_RS00570
EX31_RS00575: EX31_RS00575 - DUF554 domain-containing protein, at 122,738 to 123,451
_RS00575
EX31_RS00580: EX31_RS00580 - LysR family transcriptional regulator, at 123,539 to 124,393
_RS00580
EX31_RS00585: EX31_RS00585 - multidrug efflux SMR transporter, at 124,509 to 124,829
_RS00585
EX31_RS00590: EX31_RS00590 - helix-turn-helix transcriptional regulator, at 124,890 to 125,363
_RS00590
EX31_RS00595: EX31_RS00595 - type II toxin-antitoxin system HipA family toxin, at 125,360 to 126,709
_RS00595
Position (kb)
123
124
125 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 122.594 kb on - strand, within EX31_RS00570 at 122.614 kb on + strand, within EX31_RS00570 at 122.615 kb on - strand, within EX31_RS00570 at 122.615 kb on - strand, within EX31_RS00570 at 122.617 kb on + strand, within EX31_RS00570 at 122.672 kb on - strand at 122.715 kb on + strand at 122.734 kb on + strand at 122.735 kb on - strand at 122.735 kb on - strand at 122.743 kb on - strand at 122.752 kb on + strand at 122.752 kb on + strand at 122.753 kb on - strand at 122.753 kb on - strand at 122.753 kb on - strand at 122.969 kb on + strand, within EX31_RS00575 at 122.969 kb on + strand, within EX31_RS00575 at 122.969 kb on + strand, within EX31_RS00575 at 122.970 kb on - strand, within EX31_RS00575 at 122.970 kb on - strand, within EX31_RS00575 at 122.972 kb on - strand, within EX31_RS00575 at 122.989 kb on + strand, within EX31_RS00575 at 123.070 kb on + strand, within EX31_RS00575 at 123.194 kb on - strand, within EX31_RS00575 at 123.219 kb on + strand, within EX31_RS00575 at 123.332 kb on - strand, within EX31_RS00575 at 123.367 kb on + strand, within EX31_RS00575 at 123.374 kb on - strand, within EX31_RS00575 at 123.403 kb on - strand at 123.494 kb on + strand at 123.495 kb on - strand at 123.497 kb on - strand at 123.560 kb on + strand at 123.569 kb on - strand at 123.840 kb on - strand, within EX31_RS00580 at 124.002 kb on - strand, within EX31_RS00580 at 124.002 kb on - strand, within EX31_RS00580 at 124.145 kb on + strand, within EX31_RS00580 at 124.169 kb on + strand, within EX31_RS00580 at 124.169 kb on + strand, within EX31_RS00580 at 124.170 kb on - strand, within EX31_RS00580 at 124.348 kb on + strand at 124.348 kb on + strand at 124.392 kb on - strand at 124.421 kb on + strand at 124.421 kb on + strand at 124.421 kb on + strand at 124.422 kb on - strand at 124.469 kb on - strand at 124.497 kb on + strand at 124.522 kb on - strand at 124.604 kb on + strand, within EX31_RS00585 at 124.611 kb on - strand, within EX31_RS00585 at 124.627 kb on + strand, within EX31_RS00585 at 124.683 kb on + strand, within EX31_RS00585 at 124.683 kb on + strand, within EX31_RS00585 at 124.683 kb on + strand, within EX31_RS00585 at 124.683 kb on + strand, within EX31_RS00585 at 124.684 kb on - strand, within EX31_RS00585 at 124.754 kb on + strand, within EX31_RS00585 at 124.827 kb on + strand at 124.865 kb on - strand at 124.865 kb on - strand at 124.892 kb on - strand at 124.921 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 122,594 - EX31_RS00570 0.82 +1.5 122,614 + EX31_RS00570 0.85 +2.1 122,615 - EX31_RS00570 0.85 +0.2 122,615 - EX31_RS00570 0.85 +1.0 122,617 + EX31_RS00570 0.86 +0.2 122,672 - +1.1 122,715 + +2.1 122,734 + -0.2 122,735 - +0.1 122,735 - -1.5 122,743 - +0.3 122,752 + -0.9 122,752 + +1.2 122,753 - +1.8 122,753 - +0.5 122,753 - +0.3 122,969 + EX31_RS00575 0.32 +0.6 122,969 + EX31_RS00575 0.32 +0.2 122,969 + EX31_RS00575 0.32 +0.5 122,970 - EX31_RS00575 0.32 +0.8 122,970 - EX31_RS00575 0.32 +2.1 122,972 - EX31_RS00575 0.33 -0.2 122,989 + EX31_RS00575 0.35 +0.6 123,070 + EX31_RS00575 0.46 +1.4 123,194 - EX31_RS00575 0.64 -0.9 123,219 + EX31_RS00575 0.67 -1.2 123,332 - EX31_RS00575 0.83 -0.8 123,367 + EX31_RS00575 0.88 +2.1 123,374 - EX31_RS00575 0.89 +0.0 123,403 - +0.1 123,494 + -0.2 123,495 - -0.7 123,497 - +0.7 123,560 + -0.6 123,569 - -0.0 123,840 - EX31_RS00580 0.35 -0.8 124,002 - EX31_RS00580 0.54 -1.5 124,002 - EX31_RS00580 0.54 -1.2 124,145 + EX31_RS00580 0.71 +0.5 124,169 + EX31_RS00580 0.74 -0.5 124,169 + EX31_RS00580 0.74 -0.4 124,170 - EX31_RS00580 0.74 -1.8 124,348 + +0.1 124,348 + -0.5 124,392 - -1.4 124,421 + -0.9 124,421 + +1.3 124,421 + +0.3 124,422 - +0.3 124,469 - +0.6 124,497 + +0.3 124,522 - +0.4 124,604 + EX31_RS00585 0.30 +1.7 124,611 - EX31_RS00585 0.32 +1.3 124,627 + EX31_RS00585 0.37 -0.6 124,683 + EX31_RS00585 0.54 -1.0 124,683 + EX31_RS00585 0.54 +1.1 124,683 + EX31_RS00585 0.54 -1.1 124,683 + EX31_RS00585 0.54 +0.2 124,684 - EX31_RS00585 0.55 +0.8 124,754 + EX31_RS00585 0.76 -0.2 124,827 + -1.4 124,865 - -0.0 124,865 - +1.1 124,892 - +0.5 124,921 - +2.7
Or see this region's nucleotide sequence