Strain Fitness in Rahnella sp. WP5 around EX31_RS00580

Experiment: L-Glutamine (N); ShewMM media; no shaking

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntEX31_RS00570 and EX31_RS00575 are separated by 39 nucleotidesEX31_RS00575 and EX31_RS00580 are separated by 87 nucleotidesEX31_RS00580 and EX31_RS00585 are separated by 115 nucleotidesEX31_RS00585 and EX31_RS00590 are separated by 60 nucleotidesEX31_RS00590 and EX31_RS00595 overlap by 4 nucleotides EX31_RS00570: EX31_RS00570 - UbiX family flavin prenyltransferase, at 122,129 to 122,698 _RS00570 EX31_RS00575: EX31_RS00575 - DUF554 domain-containing protein, at 122,738 to 123,451 _RS00575 EX31_RS00580: EX31_RS00580 - LysR family transcriptional regulator, at 123,539 to 124,393 _RS00580 EX31_RS00585: EX31_RS00585 - multidrug efflux SMR transporter, at 124,509 to 124,829 _RS00585 EX31_RS00590: EX31_RS00590 - helix-turn-helix transcriptional regulator, at 124,890 to 125,363 _RS00590 EX31_RS00595: EX31_RS00595 - type II toxin-antitoxin system HipA family toxin, at 125,360 to 126,709 _RS00595 Position (kb) 123 124 125Strain fitness (log2 ratio) -2 -1 0 1 2at 122.594 kb on - strand, within EX31_RS00570at 122.614 kb on + strand, within EX31_RS00570at 122.615 kb on - strand, within EX31_RS00570at 122.615 kb on - strand, within EX31_RS00570at 122.617 kb on + strand, within EX31_RS00570at 122.672 kb on - strandat 122.715 kb on + strandat 122.734 kb on + strandat 122.735 kb on - strandat 122.735 kb on - strandat 122.743 kb on - strandat 122.752 kb on + strandat 122.752 kb on + strandat 122.753 kb on - strandat 122.753 kb on - strandat 122.753 kb on - strandat 122.969 kb on + strand, within EX31_RS00575at 122.969 kb on + strand, within EX31_RS00575at 122.969 kb on + strand, within EX31_RS00575at 122.970 kb on - strand, within EX31_RS00575at 122.970 kb on - strand, within EX31_RS00575at 122.972 kb on - strand, within EX31_RS00575at 122.989 kb on + strand, within EX31_RS00575at 123.070 kb on + strand, within EX31_RS00575at 123.194 kb on - strand, within EX31_RS00575at 123.219 kb on + strand, within EX31_RS00575at 123.332 kb on - strand, within EX31_RS00575at 123.367 kb on + strand, within EX31_RS00575at 123.374 kb on - strand, within EX31_RS00575at 123.403 kb on - strandat 123.494 kb on + strandat 123.495 kb on - strandat 123.497 kb on - strandat 123.560 kb on + strandat 123.569 kb on - strandat 123.840 kb on - strand, within EX31_RS00580at 124.002 kb on - strand, within EX31_RS00580at 124.002 kb on - strand, within EX31_RS00580at 124.145 kb on + strand, within EX31_RS00580at 124.169 kb on + strand, within EX31_RS00580at 124.169 kb on + strand, within EX31_RS00580at 124.170 kb on - strand, within EX31_RS00580at 124.348 kb on + strandat 124.348 kb on + strandat 124.392 kb on - strandat 124.421 kb on + strandat 124.421 kb on + strandat 124.421 kb on + strandat 124.422 kb on - strandat 124.469 kb on - strandat 124.497 kb on + strandat 124.522 kb on - strandat 124.604 kb on + strand, within EX31_RS00585at 124.611 kb on - strand, within EX31_RS00585at 124.627 kb on + strand, within EX31_RS00585at 124.683 kb on + strand, within EX31_RS00585at 124.683 kb on + strand, within EX31_RS00585at 124.683 kb on + strand, within EX31_RS00585at 124.683 kb on + strand, within EX31_RS00585at 124.684 kb on - strand, within EX31_RS00585at 124.754 kb on + strand, within EX31_RS00585at 124.827 kb on + strandat 124.865 kb on - strandat 124.865 kb on - strandat 124.892 kb on - strandat 124.921 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking
remove
122,594 - EX31_RS00570 0.82 +1.5
122,614 + EX31_RS00570 0.85 +2.1
122,615 - EX31_RS00570 0.85 +0.2
122,615 - EX31_RS00570 0.85 +1.0
122,617 + EX31_RS00570 0.86 +0.2
122,672 - +1.1
122,715 + +2.1
122,734 + -0.2
122,735 - +0.1
122,735 - -1.5
122,743 - +0.3
122,752 + -0.9
122,752 + +1.2
122,753 - +1.8
122,753 - +0.5
122,753 - +0.3
122,969 + EX31_RS00575 0.32 +0.6
122,969 + EX31_RS00575 0.32 +0.2
122,969 + EX31_RS00575 0.32 +0.5
122,970 - EX31_RS00575 0.32 +0.8
122,970 - EX31_RS00575 0.32 +2.1
122,972 - EX31_RS00575 0.33 -0.2
122,989 + EX31_RS00575 0.35 +0.6
123,070 + EX31_RS00575 0.46 +1.4
123,194 - EX31_RS00575 0.64 -0.9
123,219 + EX31_RS00575 0.67 -1.2
123,332 - EX31_RS00575 0.83 -0.8
123,367 + EX31_RS00575 0.88 +2.1
123,374 - EX31_RS00575 0.89 +0.0
123,403 - +0.1
123,494 + -0.2
123,495 - -0.7
123,497 - +0.7
123,560 + -0.6
123,569 - -0.0
123,840 - EX31_RS00580 0.35 -0.8
124,002 - EX31_RS00580 0.54 -1.5
124,002 - EX31_RS00580 0.54 -1.2
124,145 + EX31_RS00580 0.71 +0.5
124,169 + EX31_RS00580 0.74 -0.5
124,169 + EX31_RS00580 0.74 -0.4
124,170 - EX31_RS00580 0.74 -1.8
124,348 + +0.1
124,348 + -0.5
124,392 - -1.4
124,421 + -0.9
124,421 + +1.3
124,421 + +0.3
124,422 - +0.3
124,469 - +0.6
124,497 + +0.3
124,522 - +0.4
124,604 + EX31_RS00585 0.30 +1.7
124,611 - EX31_RS00585 0.32 +1.3
124,627 + EX31_RS00585 0.37 -0.6
124,683 + EX31_RS00585 0.54 -1.0
124,683 + EX31_RS00585 0.54 +1.1
124,683 + EX31_RS00585 0.54 -1.1
124,683 + EX31_RS00585 0.54 +0.2
124,684 - EX31_RS00585 0.55 +0.8
124,754 + EX31_RS00585 0.76 -0.2
124,827 + -1.4
124,865 - -0.0
124,865 - +1.1
124,892 - +0.5
124,921 - +2.7

Or see this region's nucleotide sequence