Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_3515 and Ac3H11_3516 are separated by 28 nucleotides Ac3H11_3516 and Ac3H11_3517 are separated by 265 nucleotides Ac3H11_3517 and Ac3H11_3518 are separated by 110 nucleotides Ac3H11_3518 and Ac3H11_3519 overlap by 17 nucleotides Ac3H11_3519 and Ac3H11_3520 overlap by 16 nucleotides
Ac3H11_3515 - putative transcriptional regulator, LysR family, at 61,414 to 62,292
_3515
Ac3H11_3516 - hypothetical protein, at 62,321 to 62,725
_3516
Ac3H11_3517 - Transcriptional regulator, LysR family, at 62,991 to 63,893
_3517
Ac3H11_3518 - Rrf2-linked NADH-flavin reductase, at 64,004 to 64,645
_3518
Ac3H11_3519 - hypothetical protein, at 64,629 to 64,796
_3519
Ac3H11_3520 - TRAP-type C4-dicarboxylate transport system, large permease component, at 64,781 to 66,061
_3520
Position (kb) 62
63
64 Strain fitness (log2 ratio) -2
-1
0
1 at 62.093 kb on + strand, within Ac3H11_3515 at 62.094 kb on - strand, within Ac3H11_3515 at 62.094 kb on - strand, within Ac3H11_3515 at 62.250 kb on - strand at 62.265 kb on - strand at 62.265 kb on - strand at 62.358 kb on + strand at 62.359 kb on - strand at 62.359 kb on - strand at 62.359 kb on - strand at 62.359 kb on - strand at 62.359 kb on - strand at 62.423 kb on + strand, within Ac3H11_3516 at 62.424 kb on - strand, within Ac3H11_3516 at 62.424 kb on - strand, within Ac3H11_3516 at 62.424 kb on - strand, within Ac3H11_3516 at 62.424 kb on - strand, within Ac3H11_3516 at 62.430 kb on + strand, within Ac3H11_3516 at 62.717 kb on - strand at 62.756 kb on + strand at 62.757 kb on - strand at 62.757 kb on - strand at 62.795 kb on + strand at 62.795 kb on + strand at 62.796 kb on - strand at 62.796 kb on - strand at 62.796 kb on - strand at 63.023 kb on - strand at 63.023 kb on - strand at 63.023 kb on - strand at 63.406 kb on + strand, within Ac3H11_3517 at 63.406 kb on + strand, within Ac3H11_3517 at 63.407 kb on - strand, within Ac3H11_3517 at 63.407 kb on - strand, within Ac3H11_3517 at 63.407 kb on - strand, within Ac3H11_3517 at 63.899 kb on - strand at 63.943 kb on - strand at 63.965 kb on + strand at 63.977 kb on - strand at 64.211 kb on + strand, within Ac3H11_3518 at 64.211 kb on + strand, within Ac3H11_3518 at 64.212 kb on - strand, within Ac3H11_3518 at 64.212 kb on - strand, within Ac3H11_3518 at 64.212 kb on - strand, within Ac3H11_3518 at 64.698 kb on + strand, within Ac3H11_3519 at 64.717 kb on - strand, within Ac3H11_3519 at 64.717 kb on - strand, within Ac3H11_3519
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 62,093 + Ac3H11_3515 0.77 +0.0 62,094 - Ac3H11_3515 0.77 -0.4 62,094 - Ac3H11_3515 0.77 -1.2 62,250 - -1.0 62,265 - -0.5 62,265 - +1.1 62,358 + -1.0 62,359 - +0.1 62,359 - +1.1 62,359 - -0.0 62,359 - -0.7 62,359 - -0.8 62,423 + Ac3H11_3516 0.25 -1.3 62,424 - Ac3H11_3516 0.25 -0.1 62,424 - Ac3H11_3516 0.25 +0.2 62,424 - Ac3H11_3516 0.25 -2.1 62,424 - Ac3H11_3516 0.25 +0.0 62,430 + Ac3H11_3516 0.27 +0.2 62,717 - -1.0 62,756 + -1.1 62,757 - -0.8 62,757 - -0.4 62,795 + -1.8 62,795 + +0.1 62,796 - -0.1 62,796 - -0.4 62,796 - -0.4 63,023 - +0.1 63,023 - -0.6 63,023 - -0.1 63,406 + Ac3H11_3517 0.46 -0.4 63,406 + Ac3H11_3517 0.46 +0.3 63,407 - Ac3H11_3517 0.46 +1.2 63,407 - Ac3H11_3517 0.46 -2.7 63,407 - Ac3H11_3517 0.46 +0.5 63,899 - +0.4 63,943 - -1.3 63,965 + -0.2 63,977 - -1.8 64,211 + Ac3H11_3518 0.32 -0.9 64,211 + Ac3H11_3518 0.32 -0.8 64,212 - Ac3H11_3518 0.32 +1.3 64,212 - Ac3H11_3518 0.32 +1.1 64,212 - Ac3H11_3518 0.32 -0.8 64,698 + Ac3H11_3519 0.41 -0.5 64,717 - Ac3H11_3519 0.52 -0.4 64,717 - Ac3H11_3519 0.52 +0.0
Or see this region's nucleotide sequence