Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_2212 and Ac3H11_2213 overlap by 4 nucleotides Ac3H11_2213 and Ac3H11_2214 are separated by 81 nucleotides Ac3H11_2214 and Ac3H11_2215 are separated by 141 nucleotides
Ac3H11_2212 - Permease of the drug/metabolite transporter (DMT) superfamily, at 444,261 to 445,187
_2212
Ac3H11_2213 - Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63), at 445,184 to 445,756
_2213
Ac3H11_2214 - ADA regulatory protein, at 445,838 to 447,400
_2214
Ac3H11_2215 - Phosphomannomutase (EC 5.4.2.8), at 447,542 to 448,930
_2215
Position (kb) 445
446
447
448 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7 at 444.997 kb on + strand, within Ac3H11_2212 at 445.303 kb on - strand, within Ac3H11_2213 at 445.434 kb on + strand, within Ac3H11_2213 at 445.434 kb on + strand, within Ac3H11_2213 at 445.434 kb on + strand, within Ac3H11_2213 at 445.435 kb on + strand, within Ac3H11_2213 at 445.552 kb on - strand, within Ac3H11_2213 at 445.552 kb on - strand, within Ac3H11_2213 at 445.623 kb on + strand, within Ac3H11_2213 at 445.624 kb on - strand, within Ac3H11_2213 at 445.749 kb on + strand at 445.750 kb on - strand at 445.872 kb on + strand at 445.872 kb on + strand at 445.873 kb on - strand at 445.873 kb on - strand at 445.873 kb on - strand at 445.873 kb on - strand at 446.016 kb on + strand, within Ac3H11_2214 at 446.016 kb on + strand, within Ac3H11_2214 at 446.115 kb on - strand, within Ac3H11_2214 at 446.793 kb on + strand, within Ac3H11_2214 at 446.922 kb on + strand, within Ac3H11_2214 at 446.922 kb on + strand, within Ac3H11_2214 at 446.922 kb on + strand, within Ac3H11_2214 at 446.922 kb on + strand, within Ac3H11_2214 at 446.922 kb on + strand, within Ac3H11_2214 at 446.922 kb on + strand, within Ac3H11_2214 at 446.923 kb on - strand, within Ac3H11_2214 at 446.923 kb on - strand, within Ac3H11_2214 at 446.923 kb on - strand, within Ac3H11_2214 at 446.923 kb on - strand, within Ac3H11_2214 at 446.936 kb on + strand, within Ac3H11_2214 at 447.255 kb on + strand at 447.255 kb on + strand at 447.255 kb on + strand at 447.255 kb on + strand at 447.255 kb on + strand at 447.255 kb on + strand at 447.255 kb on + strand at 447.256 kb on - strand at 447.256 kb on - strand at 447.256 kb on - strand at 447.327 kb on + strand at 447.400 kb on + strand at 447.400 kb on - strand at 447.401 kb on - strand at 447.401 kb on - strand at 447.401 kb on - strand at 447.401 kb on - strand at 447.401 kb on - strand at 447.495 kb on + strand at 447.495 kb on + strand at 447.495 kb on + strand at 447.495 kb on + strand at 447.495 kb on + strand at 447.527 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 444,997 + Ac3H11_2212 0.79 -1.2 445,303 - Ac3H11_2213 0.21 -1.3 445,434 + Ac3H11_2213 0.44 +0.9 445,434 + Ac3H11_2213 0.44 +0.3 445,434 + Ac3H11_2213 0.44 -0.9 445,435 + Ac3H11_2213 0.44 +0.1 445,552 - Ac3H11_2213 0.64 -1.1 445,552 - Ac3H11_2213 0.64 -0.4 445,623 + Ac3H11_2213 0.77 -2.2 445,624 - Ac3H11_2213 0.77 -0.1 445,749 + -1.2 445,750 - +0.0 445,872 + +0.7 445,872 + -1.5 445,873 - +1.6 445,873 - +2.9 445,873 - -0.5 445,873 - +1.1 446,016 + Ac3H11_2214 0.11 -0.3 446,016 + Ac3H11_2214 0.11 -0.4 446,115 - Ac3H11_2214 0.18 -2.0 446,793 + Ac3H11_2214 0.61 +1.3 446,922 + Ac3H11_2214 0.69 -0.3 446,922 + Ac3H11_2214 0.69 -0.4 446,922 + Ac3H11_2214 0.69 +0.1 446,922 + Ac3H11_2214 0.69 +0.7 446,922 + Ac3H11_2214 0.69 +0.7 446,922 + Ac3H11_2214 0.69 +7.0 446,923 - Ac3H11_2214 0.69 +0.1 446,923 - Ac3H11_2214 0.69 +0.4 446,923 - Ac3H11_2214 0.69 +1.0 446,923 - Ac3H11_2214 0.69 +0.1 446,936 + Ac3H11_2214 0.70 -1.8 447,255 + -0.2 447,255 + -0.7 447,255 + -0.4 447,255 + -0.0 447,255 + -1.7 447,255 + -0.7 447,255 + +1.0 447,256 - +1.1 447,256 - -1.5 447,256 - -2.3 447,327 + -0.6 447,400 + +2.0 447,400 - -0.5 447,401 - -0.9 447,401 - +0.3 447,401 - +1.1 447,401 - +1.1 447,401 - -0.1 447,495 + +0.4 447,495 + +1.0 447,495 + +1.9 447,495 + +0.3 447,495 + -0.8 447,527 + -0.2
Or see this region's nucleotide sequence