Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS21400 and EX31_RS21405 are separated by 501 nucleotides EX31_RS21405 and EX31_RS21410 are separated by 72 nucleotides
EX31_RS21400: EX31_RS21400 - transketolase, at 18,451 to 20,445
_RS21400
EX31_RS21405: EX31_RS21405 - erythrose-4-phosphate dehydrogenase, at 20,947 to 21,963
_RS21405
EX31_RS21410: EX31_RS21410 - phosphoglycerate kinase, at 22,036 to 23,199
_RS21410
Position (kb) 20
21
22 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 19.963 kb on + strand, within EX31_RS21400 at 20.034 kb on + strand, within EX31_RS21400 at 20.071 kb on + strand, within EX31_RS21400 at 20.144 kb on + strand, within EX31_RS21400 at 20.314 kb on + strand at 20.314 kb on + strand at 20.314 kb on + strand at 20.314 kb on + strand at 20.314 kb on + strand at 20.315 kb on - strand at 20.334 kb on + strand at 20.435 kb on + strand at 20.435 kb on + strand at 20.443 kb on + strand at 20.444 kb on - strand at 20.444 kb on - strand at 20.457 kb on + strand at 20.457 kb on + strand at 20.467 kb on + strand at 20.663 kb on + strand at 20.663 kb on + strand at 20.663 kb on + strand at 20.705 kb on + strand at 20.729 kb on - strand at 20.737 kb on + strand at 20.753 kb on + strand at 20.792 kb on + strand at 20.793 kb on - strand at 20.897 kb on + strand at 20.897 kb on + strand at 20.897 kb on + strand at 20.897 kb on + strand at 20.897 kb on + strand at 20.897 kb on + strand at 20.897 kb on + strand at 20.897 kb on + strand at 20.898 kb on - strand at 20.898 kb on - strand at 20.898 kb on - strand at 20.898 kb on - strand at 20.898 kb on - strand at 20.898 kb on - strand at 20.898 kb on - strand at 20.900 kb on + strand at 20.900 kb on + strand at 20.900 kb on + strand at 20.901 kb on - strand at 20.901 kb on - strand at 20.901 kb on - strand at 20.902 kb on + strand at 20.902 kb on + strand at 20.902 kb on + strand at 20.902 kb on + strand at 20.902 kb on + strand at 20.902 kb on + strand at 20.903 kb on - strand at 20.903 kb on - strand at 20.903 kb on - strand at 20.903 kb on - strand at 20.903 kb on - strand at 20.903 kb on - strand at 20.910 kb on - strand at 20.946 kb on + strand at 20.946 kb on + strand at 20.946 kb on + strand at 20.946 kb on + strand at 20.946 kb on + strand at 20.946 kb on + strand at 20.946 kb on + strand at 20.946 kb on + strand at 20.946 kb on + strand at 20.947 kb on - strand at 20.947 kb on - strand at 20.947 kb on - strand at 20.947 kb on - strand at 20.947 kb on - strand at 20.954 kb on + strand at 20.954 kb on + strand at 20.954 kb on + strand at 20.954 kb on + strand at 20.954 kb on + strand at 20.955 kb on - strand at 20.955 kb on - strand at 20.958 kb on + strand at 20.958 kb on + strand at 20.958 kb on + strand at 20.958 kb on + strand at 20.959 kb on - strand at 20.967 kb on + strand at 20.968 kb on - strand at 20.996 kb on + strand at 20.996 kb on + strand at 20.997 kb on - strand at 21.010 kb on + strand at 21.010 kb on + strand at 21.010 kb on + strand at 21.010 kb on + strand at 21.010 kb on + strand at 21.010 kb on + strand at 21.011 kb on - strand at 21.090 kb on + strand, within EX31_RS21405 at 21.090 kb on + strand, within EX31_RS21405 at 21.090 kb on + strand, within EX31_RS21405 at 21.090 kb on + strand, within EX31_RS21405 at 21.090 kb on + strand, within EX31_RS21405 at 21.091 kb on - strand, within EX31_RS21405 at 21.091 kb on - strand, within EX31_RS21405 at 21.091 kb on - strand, within EX31_RS21405 at 21.094 kb on + strand, within EX31_RS21405 at 21.095 kb on - strand, within EX31_RS21405 at 21.151 kb on + strand, within EX31_RS21405 at 21.152 kb on - strand, within EX31_RS21405 at 21.176 kb on + strand, within EX31_RS21405 at 21.176 kb on + strand, within EX31_RS21405 at 21.176 kb on + strand, within EX31_RS21405 at 21.177 kb on - strand, within EX31_RS21405 at 21.177 kb on - strand, within EX31_RS21405 at 21.352 kb on + strand, within EX31_RS21405 at 21.352 kb on + strand, within EX31_RS21405 at 21.352 kb on + strand, within EX31_RS21405 at 21.353 kb on - strand, within EX31_RS21405 at 21.423 kb on + strand, within EX31_RS21405 at 21.423 kb on + strand, within EX31_RS21405 at 21.423 kb on + strand, within EX31_RS21405 at 21.423 kb on + strand, within EX31_RS21405 at 21.423 kb on + strand, within EX31_RS21405 at 21.423 kb on + strand, within EX31_RS21405 at 21.423 kb on + strand, within EX31_RS21405 at 21.423 kb on + strand, within EX31_RS21405 at 21.423 kb on + strand, within EX31_RS21405 at 21.423 kb on + strand, within EX31_RS21405 at 21.423 kb on + strand, within EX31_RS21405 at 21.423 kb on + strand, within EX31_RS21405 at 21.423 kb on + strand, within EX31_RS21405 at 21.423 kb on + strand, within EX31_RS21405 at 21.423 kb on + strand, within EX31_RS21405 at 21.424 kb on - strand, within EX31_RS21405 at 21.424 kb on - strand, within EX31_RS21405 at 21.424 kb on - strand, within EX31_RS21405 at 21.424 kb on - strand, within EX31_RS21405 at 21.424 kb on - strand, within EX31_RS21405 at 21.424 kb on - strand, within EX31_RS21405 at 21.424 kb on - strand, within EX31_RS21405 at 21.424 kb on - strand, within EX31_RS21405 at 21.424 kb on - strand, within EX31_RS21405 at 21.427 kb on - strand, within EX31_RS21405 at 21.427 kb on - strand, within EX31_RS21405 at 21.526 kb on + strand, within EX31_RS21405 at 21.526 kb on + strand, within EX31_RS21405 at 21.526 kb on + strand, within EX31_RS21405 at 21.527 kb on - strand, within EX31_RS21405 at 21.527 kb on - strand, within EX31_RS21405 at 21.527 kb on - strand, within EX31_RS21405 at 21.539 kb on + strand, within EX31_RS21405 at 21.583 kb on - strand, within EX31_RS21405 at 21.692 kb on + strand, within EX31_RS21405 at 21.692 kb on + strand, within EX31_RS21405 at 21.692 kb on + strand, within EX31_RS21405 at 21.692 kb on + strand, within EX31_RS21405 at 21.693 kb on - strand, within EX31_RS21405 at 21.693 kb on - strand, within EX31_RS21405 at 21.693 kb on - strand, within EX31_RS21405 at 21.716 kb on + strand, within EX31_RS21405 at 21.716 kb on + strand, within EX31_RS21405 at 21.768 kb on + strand, within EX31_RS21405 at 21.768 kb on + strand, within EX31_RS21405 at 21.778 kb on + strand, within EX31_RS21405 at 21.780 kb on + strand, within EX31_RS21405 at 21.788 kb on + strand, within EX31_RS21405 at 21.788 kb on + strand, within EX31_RS21405 at 21.788 kb on + strand, within EX31_RS21405 at 21.794 kb on + strand, within EX31_RS21405 at 21.794 kb on + strand, within EX31_RS21405 at 21.794 kb on + strand, within EX31_RS21405 at 21.836 kb on + strand, within EX31_RS21405 at 21.836 kb on + strand, within EX31_RS21405 at 21.836 kb on + strand, within EX31_RS21405 at 21.836 kb on + strand, within EX31_RS21405 at 21.836 kb on + strand, within EX31_RS21405 at 21.857 kb on + strand, within EX31_RS21405 at 21.857 kb on + strand, within EX31_RS21405 at 21.930 kb on + strand at 21.930 kb on + strand at 21.930 kb on + strand at 22.009 kb on + strand at 22.009 kb on + strand at 22.009 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 19,963 + EX31_RS21400 0.76 +2.6 20,034 + EX31_RS21400 0.79 +0.2 20,071 + EX31_RS21400 0.81 +1.4 20,144 + EX31_RS21400 0.85 -1.9 20,314 + +0.4 20,314 + -0.3 20,314 + -0.0 20,314 + +1.4 20,314 + -0.0 20,315 - -0.3 20,334 + -0.8 20,435 + -0.1 20,435 + -0.0 20,443 + -2.2 20,444 - -1.0 20,444 - +1.3 20,457 + -1.7 20,457 + +0.1 20,467 + -0.6 20,663 + -0.2 20,663 + +0.3 20,663 + +0.6 20,705 + +0.5 20,729 - -0.2 20,737 + +0.1 20,753 + +0.1 20,792 + +0.1 20,793 - -1.2 20,897 + +0.6 20,897 + -0.0 20,897 + -0.2 20,897 + -0.5 20,897 + -0.8 20,897 + -0.6 20,897 + -2.1 20,897 + -0.9 20,898 - +0.1 20,898 - +0.4 20,898 - -0.7 20,898 - +0.7 20,898 - -0.6 20,898 - -1.1 20,898 - -1.1 20,900 + -0.3 20,900 + -0.9 20,900 + -1.0 20,901 - -0.5 20,901 - -0.3 20,901 - -1.1 20,902 + -0.7 20,902 + -1.5 20,902 + +0.4 20,902 + -0.9 20,902 + -1.3 20,902 + +0.3 20,903 - -0.5 20,903 - +0.2 20,903 - +0.4 20,903 - -0.5 20,903 - -1.7 20,903 - +0.8 20,910 - -1.7 20,946 + -1.4 20,946 + -1.1 20,946 + +1.2 20,946 + -1.2 20,946 + -2.0 20,946 + -0.5 20,946 + -1.2 20,946 + -1.2 20,946 + -0.1 20,947 - -1.7 20,947 - -0.4 20,947 - -2.2 20,947 - -1.6 20,947 - -1.8 20,954 + -1.5 20,954 + +0.6 20,954 + -1.2 20,954 + +0.4 20,954 + -0.6 20,955 - +3.0 20,955 - -0.9 20,958 + -0.5 20,958 + -0.8 20,958 + -0.6 20,958 + -0.6 20,959 - -0.6 20,967 + -1.3 20,968 - -0.0 20,996 + -1.0 20,996 + -2.6 20,997 - -0.6 21,010 + -0.7 21,010 + -0.2 21,010 + -0.6 21,010 + -0.5 21,010 + -0.4 21,010 + -1.1 21,011 - +0.2 21,090 + EX31_RS21405 0.14 +1.1 21,090 + EX31_RS21405 0.14 -1.3 21,090 + EX31_RS21405 0.14 -1.2 21,090 + EX31_RS21405 0.14 -1.7 21,090 + EX31_RS21405 0.14 -1.8 21,091 - EX31_RS21405 0.14 -0.5 21,091 - EX31_RS21405 0.14 -0.5 21,091 - EX31_RS21405 0.14 +0.3 21,094 + EX31_RS21405 0.14 -1.4 21,095 - EX31_RS21405 0.15 -1.3 21,151 + EX31_RS21405 0.20 -1.5 21,152 - EX31_RS21405 0.20 +0.6 21,176 + EX31_RS21405 0.23 -0.3 21,176 + EX31_RS21405 0.23 -0.7 21,176 + EX31_RS21405 0.23 -1.0 21,177 - EX31_RS21405 0.23 -0.6 21,177 - EX31_RS21405 0.23 -1.8 21,352 + EX31_RS21405 0.40 -0.7 21,352 + EX31_RS21405 0.40 -1.9 21,352 + EX31_RS21405 0.40 +0.1 21,353 - EX31_RS21405 0.40 -0.4 21,423 + EX31_RS21405 0.47 -0.3 21,423 + EX31_RS21405 0.47 -0.1 21,423 + EX31_RS21405 0.47 -0.1 21,423 + EX31_RS21405 0.47 -1.5 21,423 + EX31_RS21405 0.47 +0.1 21,423 + EX31_RS21405 0.47 -0.8 21,423 + EX31_RS21405 0.47 -1.0 21,423 + EX31_RS21405 0.47 -0.4 21,423 + EX31_RS21405 0.47 -0.4 21,423 + EX31_RS21405 0.47 -0.4 21,423 + EX31_RS21405 0.47 -0.0 21,423 + EX31_RS21405 0.47 +1.4 21,423 + EX31_RS21405 0.47 +0.1 21,423 + EX31_RS21405 0.47 -0.8 21,423 + EX31_RS21405 0.47 -0.5 21,424 - EX31_RS21405 0.47 -1.4 21,424 - EX31_RS21405 0.47 -0.5 21,424 - EX31_RS21405 0.47 -1.3 21,424 - EX31_RS21405 0.47 -0.3 21,424 - EX31_RS21405 0.47 -0.8 21,424 - EX31_RS21405 0.47 +0.1 21,424 - EX31_RS21405 0.47 +1.2 21,424 - EX31_RS21405 0.47 -0.8 21,424 - EX31_RS21405 0.47 -0.2 21,427 - EX31_RS21405 0.47 -1.6 21,427 - EX31_RS21405 0.47 +1.3 21,526 + EX31_RS21405 0.57 -0.5 21,526 + EX31_RS21405 0.57 +1.3 21,526 + EX31_RS21405 0.57 +1.0 21,527 - EX31_RS21405 0.57 +0.5 21,527 - EX31_RS21405 0.57 -0.1 21,527 - EX31_RS21405 0.57 +1.3 21,539 + EX31_RS21405 0.58 +0.8 21,583 - EX31_RS21405 0.63 -3.1 21,692 + EX31_RS21405 0.73 -1.4 21,692 + EX31_RS21405 0.73 -0.4 21,692 + EX31_RS21405 0.73 -0.4 21,692 + EX31_RS21405 0.73 -0.4 21,693 - EX31_RS21405 0.73 +0.3 21,693 - EX31_RS21405 0.73 -0.4 21,693 - EX31_RS21405 0.73 -2.6 21,716 + EX31_RS21405 0.76 -1.2 21,716 + EX31_RS21405 0.76 -1.2 21,768 + EX31_RS21405 0.81 +0.4 21,768 + EX31_RS21405 0.81 -0.1 21,778 + EX31_RS21405 0.82 -1.3 21,780 + EX31_RS21405 0.82 -0.4 21,788 + EX31_RS21405 0.83 +0.6 21,788 + EX31_RS21405 0.83 -0.6 21,788 + EX31_RS21405 0.83 -0.0 21,794 + EX31_RS21405 0.83 +0.1 21,794 + EX31_RS21405 0.83 -0.6 21,794 + EX31_RS21405 0.83 -0.3 21,836 + EX31_RS21405 0.87 -0.0 21,836 + EX31_RS21405 0.87 -0.1 21,836 + EX31_RS21405 0.87 -1.0 21,836 + EX31_RS21405 0.87 -0.4 21,836 + EX31_RS21405 0.87 -0.2 21,857 + EX31_RS21405 0.89 -0.0 21,857 + EX31_RS21405 0.89 -0.7 21,930 + -0.2 21,930 + +2.0 21,930 + -1.2 22,009 + -1.3 22,009 + -1.0 22,009 + -0.5
Or see this region's nucleotide sequence