Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS20490 and EX31_RS20495 are separated by 211 nucleotides EX31_RS20495 and EX31_RS20500 are separated by 11 nucleotides EX31_RS20500 and EX31_RS20505 are separated by 13 nucleotides
EX31_RS20490: EX31_RS20490 - ribosome biogenesis GTPase Der, at 734,436 to 735,920
_RS20490
EX31_RS20495: EX31_RS20495 - outer membrane protein assembly factor BamB, at 736,132 to 737,313
_RS20495
EX31_RS20500: EX31_RS20500 - YfgM family protein, at 737,325 to 737,945
_RS20500
EX31_RS20505: EX31_RS20505 - histidine--tRNA ligase, at 737,959 to 739,233
_RS20505
Position (kb) 736
737
738 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 735.997 kb on - strand at 736.131 kb on - strand at 736.149 kb on - strand at 736.305 kb on - strand, within EX31_RS20495 at 736.305 kb on - strand, within EX31_RS20495 at 736.305 kb on - strand, within EX31_RS20495 at 736.305 kb on - strand, within EX31_RS20495 at 736.314 kb on - strand, within EX31_RS20495 at 736.314 kb on - strand, within EX31_RS20495 at 736.314 kb on - strand, within EX31_RS20495 at 736.336 kb on - strand, within EX31_RS20495 at 736.420 kb on - strand, within EX31_RS20495 at 736.422 kb on - strand, within EX31_RS20495 at 736.422 kb on - strand, within EX31_RS20495 at 736.422 kb on - strand, within EX31_RS20495 at 736.422 kb on - strand, within EX31_RS20495 at 736.422 kb on - strand, within EX31_RS20495 at 736.422 kb on - strand, within EX31_RS20495 at 736.422 kb on - strand, within EX31_RS20495 at 736.422 kb on - strand, within EX31_RS20495 at 736.422 kb on - strand, within EX31_RS20495 at 736.422 kb on - strand, within EX31_RS20495 at 736.422 kb on - strand, within EX31_RS20495 at 736.422 kb on - strand, within EX31_RS20495 at 736.422 kb on - strand, within EX31_RS20495 at 736.422 kb on - strand, within EX31_RS20495 at 736.422 kb on - strand, within EX31_RS20495 at 736.422 kb on - strand, within EX31_RS20495 at 736.422 kb on - strand, within EX31_RS20495 at 736.422 kb on - strand, within EX31_RS20495 at 736.422 kb on - strand, within EX31_RS20495 at 736.422 kb on - strand, within EX31_RS20495 at 736.422 kb on - strand, within EX31_RS20495 at 736.422 kb on - strand, within EX31_RS20495 at 736.422 kb on - strand, within EX31_RS20495 at 736.422 kb on - strand, within EX31_RS20495 at 736.422 kb on - strand, within EX31_RS20495 at 736.422 kb on - strand, within EX31_RS20495 at 736.422 kb on - strand, within EX31_RS20495 at 736.426 kb on - strand, within EX31_RS20495 at 736.426 kb on - strand, within EX31_RS20495 at 736.426 kb on - strand, within EX31_RS20495 at 736.480 kb on - strand, within EX31_RS20495 at 736.480 kb on - strand, within EX31_RS20495 at 736.480 kb on - strand, within EX31_RS20495 at 736.524 kb on - strand, within EX31_RS20495 at 736.524 kb on - strand, within EX31_RS20495 at 736.524 kb on - strand, within EX31_RS20495 at 736.536 kb on - strand, within EX31_RS20495 at 736.536 kb on - strand, within EX31_RS20495 at 736.618 kb on - strand, within EX31_RS20495 at 736.618 kb on - strand, within EX31_RS20495 at 736.618 kb on - strand, within EX31_RS20495 at 736.618 kb on - strand, within EX31_RS20495 at 736.618 kb on - strand, within EX31_RS20495 at 736.655 kb on - strand, within EX31_RS20495 at 736.804 kb on - strand, within EX31_RS20495 at 736.804 kb on - strand, within EX31_RS20495 at 736.804 kb on - strand, within EX31_RS20495 at 736.804 kb on - strand, within EX31_RS20495 at 736.894 kb on - strand, within EX31_RS20495 at 736.916 kb on + strand, within EX31_RS20495 at 736.944 kb on - strand, within EX31_RS20495 at 736.944 kb on - strand, within EX31_RS20495 at 736.944 kb on - strand, within EX31_RS20495 at 737.245 kb on - strand at 737.264 kb on - strand at 737.288 kb on - strand at 737.288 kb on - strand at 737.335 kb on - strand at 737.335 kb on - strand at 737.560 kb on - strand, within EX31_RS20500 at 737.560 kb on - strand, within EX31_RS20500 at 737.560 kb on - strand, within EX31_RS20500 at 737.560 kb on - strand, within EX31_RS20500 at 737.560 kb on - strand, within EX31_RS20500 at 737.560 kb on - strand, within EX31_RS20500 at 737.560 kb on - strand, within EX31_RS20500 at 737.560 kb on - strand, within EX31_RS20500 at 737.560 kb on - strand, within EX31_RS20500 at 737.560 kb on - strand, within EX31_RS20500 at 737.560 kb on - strand, within EX31_RS20500 at 737.560 kb on - strand, within EX31_RS20500 at 737.560 kb on - strand, within EX31_RS20500 at 737.583 kb on - strand, within EX31_RS20500 at 737.583 kb on - strand, within EX31_RS20500 at 737.614 kb on - strand, within EX31_RS20500 at 737.614 kb on - strand, within EX31_RS20500 at 737.614 kb on - strand, within EX31_RS20500 at 737.690 kb on - strand, within EX31_RS20500 at 737.766 kb on - strand, within EX31_RS20500 at 737.905 kb on - strand at 737.905 kb on - strand at 737.905 kb on - strand at 737.905 kb on - strand at 737.905 kb on - strand at 737.936 kb on - strand at 737.936 kb on - strand at 737.936 kb on - strand at 737.936 kb on - strand at 737.936 kb on - strand at 737.936 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 735,997 - +0.4 736,131 - +0.3 736,149 - -0.2 736,305 - EX31_RS20495 0.15 -4.3 736,305 - EX31_RS20495 0.15 -3.1 736,305 - EX31_RS20495 0.15 -1.1 736,305 - EX31_RS20495 0.15 -3.2 736,314 - EX31_RS20495 0.15 -2.5 736,314 - EX31_RS20495 0.15 -2.0 736,314 - EX31_RS20495 0.15 -0.7 736,336 - EX31_RS20495 0.17 -2.2 736,420 - EX31_RS20495 0.24 -1.5 736,422 - EX31_RS20495 0.25 -2.3 736,422 - EX31_RS20495 0.25 -2.6 736,422 - EX31_RS20495 0.25 -3.8 736,422 - EX31_RS20495 0.25 -2.9 736,422 - EX31_RS20495 0.25 -3.1 736,422 - EX31_RS20495 0.25 -1.9 736,422 - EX31_RS20495 0.25 -2.6 736,422 - EX31_RS20495 0.25 -1.1 736,422 - EX31_RS20495 0.25 -1.2 736,422 - EX31_RS20495 0.25 -2.2 736,422 - EX31_RS20495 0.25 -3.0 736,422 - EX31_RS20495 0.25 -2.1 736,422 - EX31_RS20495 0.25 +0.1 736,422 - EX31_RS20495 0.25 -3.3 736,422 - EX31_RS20495 0.25 -1.4 736,422 - EX31_RS20495 0.25 -2.8 736,422 - EX31_RS20495 0.25 -3.2 736,422 - EX31_RS20495 0.25 -2.7 736,422 - EX31_RS20495 0.25 -1.8 736,422 - EX31_RS20495 0.25 -3.5 736,422 - EX31_RS20495 0.25 -0.2 736,422 - EX31_RS20495 0.25 -3.7 736,422 - EX31_RS20495 0.25 -1.7 736,422 - EX31_RS20495 0.25 -3.9 736,422 - EX31_RS20495 0.25 -1.1 736,422 - EX31_RS20495 0.25 -1.6 736,422 - EX31_RS20495 0.25 -0.5 736,426 - EX31_RS20495 0.25 -2.3 736,426 - EX31_RS20495 0.25 -2.4 736,426 - EX31_RS20495 0.25 -2.2 736,480 - EX31_RS20495 0.29 -2.3 736,480 - EX31_RS20495 0.29 -3.3 736,480 - EX31_RS20495 0.29 -2.1 736,524 - EX31_RS20495 0.33 -1.6 736,524 - EX31_RS20495 0.33 -1.5 736,524 - EX31_RS20495 0.33 -0.5 736,536 - EX31_RS20495 0.34 -2.4 736,536 - EX31_RS20495 0.34 -3.8 736,618 - EX31_RS20495 0.41 -2.9 736,618 - EX31_RS20495 0.41 -1.2 736,618 - EX31_RS20495 0.41 -4.0 736,618 - EX31_RS20495 0.41 +2.1 736,618 - EX31_RS20495 0.41 -3.3 736,655 - EX31_RS20495 0.44 -2.4 736,804 - EX31_RS20495 0.57 -1.7 736,804 - EX31_RS20495 0.57 -0.9 736,804 - EX31_RS20495 0.57 -1.6 736,804 - EX31_RS20495 0.57 -2.1 736,894 - EX31_RS20495 0.64 -4.1 736,916 + EX31_RS20495 0.66 -0.9 736,944 - EX31_RS20495 0.69 -3.0 736,944 - EX31_RS20495 0.69 -2.9 736,944 - EX31_RS20495 0.69 -2.2 737,245 - -2.4 737,264 - -2.0 737,288 - -3.9 737,288 - -1.7 737,335 - +0.1 737,335 - -1.4 737,560 - EX31_RS20500 0.38 +0.8 737,560 - EX31_RS20500 0.38 +0.2 737,560 - EX31_RS20500 0.38 +0.1 737,560 - EX31_RS20500 0.38 +0.4 737,560 - EX31_RS20500 0.38 +0.7 737,560 - EX31_RS20500 0.38 -0.3 737,560 - EX31_RS20500 0.38 +0.6 737,560 - EX31_RS20500 0.38 -0.3 737,560 - EX31_RS20500 0.38 -0.4 737,560 - EX31_RS20500 0.38 -0.7 737,560 - EX31_RS20500 0.38 -0.1 737,560 - EX31_RS20500 0.38 +0.1 737,560 - EX31_RS20500 0.38 -0.5 737,583 - EX31_RS20500 0.42 -0.3 737,583 - EX31_RS20500 0.42 +0.3 737,614 - EX31_RS20500 0.47 -0.3 737,614 - EX31_RS20500 0.47 -0.3 737,614 - EX31_RS20500 0.47 +0.4 737,690 - EX31_RS20500 0.59 -0.9 737,766 - EX31_RS20500 0.71 -0.2 737,905 - +1.5 737,905 - +0.5 737,905 - +1.0 737,905 - -0.3 737,905 - -1.0 737,936 - -0.0 737,936 - +1.0 737,936 - +0.6 737,936 - -0.2 737,936 - -0.2 737,936 - -0.5
Or see this region's nucleotide sequence