Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS17525 and EX31_RS17530 are separated by 151 nucleotides EX31_RS17530 and EX31_RS17535 are separated by 298 nucleotides EX31_RS17535 and EX31_RS17540 are separated by 143 nucleotides
EX31_RS17525: EX31_RS17525 - pyridoxal phosphate-dependent aminotransferase, at 57,499 to 58,707
_RS17525
EX31_RS17530: EX31_RS17530 - tRNA-Asn, at 58,859 to 58,934
_RS17530
EX31_RS17535: EX31_RS17535 - DUF1028 domain-containing protein, at 59,233 to 59,883
_RS17535
EX31_RS17540: EX31_RS17540 - ABC transporter substrate-binding protein, at 60,027 to 61,601
_RS17540
Position (kb)
59
60 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 58.477 kb on + strand, within EX31_RS17525 at 58.478 kb on - strand, within EX31_RS17525 at 58.488 kb on + strand, within EX31_RS17525 at 58.510 kb on - strand, within EX31_RS17525 at 58.510 kb on - strand, within EX31_RS17525 at 58.561 kb on + strand, within EX31_RS17525 at 58.562 kb on - strand, within EX31_RS17525 at 58.562 kb on - strand, within EX31_RS17525 at 58.562 kb on - strand, within EX31_RS17525 at 58.562 kb on - strand, within EX31_RS17525 at 58.621 kb on + strand at 58.625 kb on + strand at 58.626 kb on - strand at 58.626 kb on - strand at 58.629 kb on + strand at 58.629 kb on + strand at 58.629 kb on + strand at 58.629 kb on + strand at 58.629 kb on + strand at 58.637 kb on - strand at 58.637 kb on - strand at 58.777 kb on + strand at 58.797 kb on + strand at 58.962 kb on + strand at 58.982 kb on + strand at 59.004 kb on - strand at 59.131 kb on + strand at 59.131 kb on + strand at 59.131 kb on + strand at 59.132 kb on - strand at 59.215 kb on - strand at 59.224 kb on + strand at 59.225 kb on - strand at 59.401 kb on - strand, within EX31_RS17535 at 59.530 kb on + strand, within EX31_RS17535 at 59.531 kb on - strand, within EX31_RS17535 at 59.635 kb on + strand, within EX31_RS17535 at 59.636 kb on - strand, within EX31_RS17535 at 59.636 kb on - strand, within EX31_RS17535 at 59.685 kb on + strand, within EX31_RS17535 at 59.685 kb on + strand, within EX31_RS17535 at 59.685 kb on + strand, within EX31_RS17535 at 59.685 kb on + strand, within EX31_RS17535 at 59.685 kb on + strand, within EX31_RS17535 at 59.685 kb on + strand, within EX31_RS17535 at 59.685 kb on + strand, within EX31_RS17535 at 59.685 kb on + strand, within EX31_RS17535 at 59.685 kb on + strand, within EX31_RS17535 at 59.686 kb on - strand, within EX31_RS17535 at 59.686 kb on - strand, within EX31_RS17535 at 59.686 kb on - strand, within EX31_RS17535 at 59.686 kb on - strand, within EX31_RS17535 at 59.686 kb on - strand, within EX31_RS17535 at 59.686 kb on - strand, within EX31_RS17535 at 59.686 kb on - strand, within EX31_RS17535 at 59.686 kb on - strand, within EX31_RS17535 at 59.686 kb on - strand, within EX31_RS17535 at 59.686 kb on - strand, within EX31_RS17535 at 59.686 kb on - strand, within EX31_RS17535 at 59.686 kb on - strand, within EX31_RS17535 at 59.687 kb on + strand, within EX31_RS17535 at 59.687 kb on + strand, within EX31_RS17535 at 59.687 kb on + strand, within EX31_RS17535 at 59.688 kb on - strand, within EX31_RS17535 at 59.688 kb on - strand, within EX31_RS17535 at 59.688 kb on - strand, within EX31_RS17535 at 59.710 kb on + strand, within EX31_RS17535 at 59.710 kb on + strand, within EX31_RS17535 at 59.711 kb on - strand, within EX31_RS17535 at 59.711 kb on - strand, within EX31_RS17535 at 59.711 kb on - strand, within EX31_RS17535 at 59.792 kb on - strand, within EX31_RS17535 at 59.883 kb on + strand at 59.884 kb on - strand at 59.887 kb on + strand at 59.887 kb on + strand at 59.887 kb on + strand at 59.887 kb on + strand at 59.888 kb on - strand at 59.888 kb on - strand at 59.888 kb on - strand at 59.999 kb on + strand at 59.999 kb on + strand at 60.000 kb on - strand at 60.000 kb on - strand at 60.000 kb on - strand at 60.008 kb on - strand at 60.008 kb on - strand at 60.008 kb on - strand at 60.053 kb on + strand at 60.053 kb on + strand at 60.053 kb on + strand at 60.053 kb on + strand at 60.054 kb on - strand at 60.054 kb on - strand at 60.054 kb on - strand at 60.054 kb on - strand at 60.214 kb on - strand, within EX31_RS17540 at 60.299 kb on + strand, within EX31_RS17540 at 60.300 kb on - strand, within EX31_RS17540 at 60.313 kb on - strand, within EX31_RS17540 at 60.387 kb on - strand, within EX31_RS17540 at 60.387 kb on - strand, within EX31_RS17540 at 60.483 kb on - strand, within EX31_RS17540 at 60.678 kb on + strand, within EX31_RS17540 at 60.738 kb on - strand, within EX31_RS17540 at 60.781 kb on + strand, within EX31_RS17540 at 60.781 kb on + strand, within EX31_RS17540 at 60.781 kb on + strand, within EX31_RS17540 at 60.882 kb on - strand, within EX31_RS17540 at 60.882 kb on - strand, within EX31_RS17540
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 58,477 + EX31_RS17525 0.81 +1.3 58,478 - EX31_RS17525 0.81 +0.4 58,488 + EX31_RS17525 0.82 +1.3 58,510 - EX31_RS17525 0.84 +0.5 58,510 - EX31_RS17525 0.84 -0.5 58,561 + EX31_RS17525 0.88 +0.3 58,562 - EX31_RS17525 0.88 +1.7 58,562 - EX31_RS17525 0.88 +1.2 58,562 - EX31_RS17525 0.88 -1.3 58,562 - EX31_RS17525 0.88 +0.5 58,621 + +0.1 58,625 + -2.5 58,626 - -0.8 58,626 - -0.7 58,629 + -2.8 58,629 + -1.3 58,629 + -0.7 58,629 + +0.7 58,629 + +0.9 58,637 - -1.0 58,637 - +0.8 58,777 + +0.3 58,797 + +0.5 58,962 + +0.4 58,982 + +0.6 59,004 - -0.8 59,131 + -0.0 59,131 + +1.3 59,131 + +0.9 59,132 - -1.9 59,215 - -0.5 59,224 + +0.5 59,225 - +0.1 59,401 - EX31_RS17535 0.26 -0.7 59,530 + EX31_RS17535 0.46 -0.9 59,531 - EX31_RS17535 0.46 +0.3 59,635 + EX31_RS17535 0.62 -0.1 59,636 - EX31_RS17535 0.62 -0.9 59,636 - EX31_RS17535 0.62 +0.6 59,685 + EX31_RS17535 0.69 +0.3 59,685 + EX31_RS17535 0.69 -0.2 59,685 + EX31_RS17535 0.69 -2.4 59,685 + EX31_RS17535 0.69 -1.3 59,685 + EX31_RS17535 0.69 -0.8 59,685 + EX31_RS17535 0.69 +1.0 59,685 + EX31_RS17535 0.69 +0.3 59,685 + EX31_RS17535 0.69 -0.5 59,685 + EX31_RS17535 0.69 -0.1 59,686 - EX31_RS17535 0.70 +0.8 59,686 - EX31_RS17535 0.70 +0.6 59,686 - EX31_RS17535 0.70 -0.6 59,686 - EX31_RS17535 0.70 -0.9 59,686 - EX31_RS17535 0.70 +0.1 59,686 - EX31_RS17535 0.70 -0.6 59,686 - EX31_RS17535 0.70 +0.4 59,686 - EX31_RS17535 0.70 +0.3 59,686 - EX31_RS17535 0.70 -1.0 59,686 - EX31_RS17535 0.70 +0.2 59,686 - EX31_RS17535 0.70 -0.7 59,686 - EX31_RS17535 0.70 -0.6 59,687 + EX31_RS17535 0.70 +0.5 59,687 + EX31_RS17535 0.70 -1.0 59,687 + EX31_RS17535 0.70 +0.7 59,688 - EX31_RS17535 0.70 +0.2 59,688 - EX31_RS17535 0.70 -1.4 59,688 - EX31_RS17535 0.70 +0.3 59,710 + EX31_RS17535 0.73 -0.8 59,710 + EX31_RS17535 0.73 +0.6 59,711 - EX31_RS17535 0.73 +0.3 59,711 - EX31_RS17535 0.73 -1.1 59,711 - EX31_RS17535 0.73 +0.3 59,792 - EX31_RS17535 0.86 -2.3 59,883 + +2.1 59,884 - -0.1 59,887 + -0.3 59,887 + +1.3 59,887 + -0.8 59,887 + +1.8 59,888 - +1.4 59,888 - +1.9 59,888 - +0.1 59,999 + -0.1 59,999 + +0.2 60,000 - -1.3 60,000 - -0.1 60,000 - +0.2 60,008 - -0.3 60,008 - +0.6 60,008 - +0.3 60,053 + +1.5 60,053 + +0.7 60,053 + -0.5 60,053 + -0.4 60,054 - +0.9 60,054 - -0.1 60,054 - +0.4 60,054 - +0.6 60,214 - EX31_RS17540 0.12 +1.4 60,299 + EX31_RS17540 0.17 +0.4 60,300 - EX31_RS17540 0.17 +0.3 60,313 - EX31_RS17540 0.18 -1.1 60,387 - EX31_RS17540 0.23 -0.8 60,387 - EX31_RS17540 0.23 -0.4 60,483 - EX31_RS17540 0.29 +0.3 60,678 + EX31_RS17540 0.41 +0.5 60,738 - EX31_RS17540 0.45 +1.4 60,781 + EX31_RS17540 0.48 +0.2 60,781 + EX31_RS17540 0.48 +0.9 60,781 + EX31_RS17540 0.48 +0.5 60,882 - EX31_RS17540 0.54 +1.3 60,882 - EX31_RS17540 0.54 +0.9
Or see this region's nucleotide sequence