Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS14180 and EX31_RS14185 are separated by 83 nucleotides EX31_RS14185 and EX31_RS14190 are separated by 131 nucleotides
EX31_RS14180: EX31_RS14180 - argininosuccinate lyase, at 12,033 to 13,406
_RS14180
EX31_RS14185: EX31_RS14185 - acetylglutamate kinase, at 13,490 to 14,263
_RS14185
EX31_RS14190: EX31_RS14190 - N-acetyl-gamma-glutamyl-phosphate reductase, at 14,395 to 15,399
_RS14190
Position (kb) 13
14
15 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3 at 12.510 kb on + strand, within EX31_RS14180 at 12.510 kb on + strand, within EX31_RS14180 at 12.511 kb on - strand, within EX31_RS14180 at 12.511 kb on - strand, within EX31_RS14180 at 12.629 kb on + strand, within EX31_RS14180 at 12.629 kb on + strand, within EX31_RS14180 at 12.671 kb on + strand, within EX31_RS14180 at 12.671 kb on + strand, within EX31_RS14180 at 12.671 kb on + strand, within EX31_RS14180 at 12.671 kb on + strand, within EX31_RS14180 at 12.672 kb on - strand, within EX31_RS14180 at 12.672 kb on - strand, within EX31_RS14180 at 12.672 kb on - strand, within EX31_RS14180 at 12.672 kb on - strand, within EX31_RS14180 at 12.738 kb on - strand, within EX31_RS14180 at 12.892 kb on + strand, within EX31_RS14180 at 12.892 kb on + strand, within EX31_RS14180 at 12.892 kb on + strand, within EX31_RS14180 at 12.892 kb on + strand, within EX31_RS14180 at 12.892 kb on + strand, within EX31_RS14180 at 12.892 kb on + strand, within EX31_RS14180 at 12.892 kb on + strand, within EX31_RS14180 at 12.892 kb on + strand, within EX31_RS14180 at 12.893 kb on - strand, within EX31_RS14180 at 12.893 kb on - strand, within EX31_RS14180 at 12.893 kb on - strand, within EX31_RS14180 at 13.002 kb on + strand, within EX31_RS14180 at 13.002 kb on + strand, within EX31_RS14180 at 13.003 kb on - strand, within EX31_RS14180 at 13.043 kb on + strand, within EX31_RS14180 at 13.043 kb on + strand, within EX31_RS14180 at 13.044 kb on - strand, within EX31_RS14180 at 13.044 kb on - strand, within EX31_RS14180 at 13.044 kb on - strand, within EX31_RS14180 at 13.044 kb on - strand, within EX31_RS14180 at 13.137 kb on - strand, within EX31_RS14180 at 13.151 kb on + strand, within EX31_RS14180 at 13.151 kb on + strand, within EX31_RS14180 at 13.151 kb on + strand, within EX31_RS14180 at 13.157 kb on + strand, within EX31_RS14180 at 13.158 kb on - strand, within EX31_RS14180 at 13.158 kb on - strand, within EX31_RS14180 at 13.158 kb on - strand, within EX31_RS14180 at 13.158 kb on - strand, within EX31_RS14180 at 13.176 kb on + strand, within EX31_RS14180 at 13.176 kb on + strand, within EX31_RS14180 at 13.177 kb on - strand, within EX31_RS14180 at 13.260 kb on + strand, within EX31_RS14180 at 13.260 kb on + strand, within EX31_RS14180 at 13.260 kb on + strand, within EX31_RS14180 at 13.261 kb on - strand, within EX31_RS14180 at 13.261 kb on - strand, within EX31_RS14180 at 13.261 kb on - strand, within EX31_RS14180 at 13.261 kb on - strand, within EX31_RS14180 at 13.261 kb on - strand, within EX31_RS14180 at 13.293 kb on + strand at 13.293 kb on + strand at 13.293 kb on + strand at 13.383 kb on - strand at 13.427 kb on + strand at 13.444 kb on + strand at 13.444 kb on + strand at 13.445 kb on - strand at 13.445 kb on - strand at 13.445 kb on - strand at 13.445 kb on - strand at 13.445 kb on - strand at 13.445 kb on - strand at 13.445 kb on - strand at 13.466 kb on + strand at 13.466 kb on + strand at 13.491 kb on + strand at 13.501 kb on + strand at 13.501 kb on + strand at 13.501 kb on + strand at 13.501 kb on + strand at 13.501 kb on + strand at 13.501 kb on + strand at 13.501 kb on + strand at 13.501 kb on + strand at 13.502 kb on - strand at 13.502 kb on - strand at 13.502 kb on - strand at 13.502 kb on - strand at 13.502 kb on - strand at 13.502 kb on - strand at 13.502 kb on - strand at 13.502 kb on - strand at 13.567 kb on + strand at 13.567 kb on + strand at 13.568 kb on - strand, within EX31_RS14185 at 13.639 kb on + strand, within EX31_RS14185 at 13.640 kb on - strand, within EX31_RS14185 at 13.640 kb on - strand, within EX31_RS14185 at 13.640 kb on - strand, within EX31_RS14185 at 13.642 kb on + strand, within EX31_RS14185 at 13.642 kb on + strand, within EX31_RS14185 at 13.642 kb on + strand, within EX31_RS14185 at 13.642 kb on + strand, within EX31_RS14185 at 13.642 kb on + strand, within EX31_RS14185 at 13.690 kb on + strand, within EX31_RS14185 at 13.690 kb on + strand, within EX31_RS14185 at 13.690 kb on + strand, within EX31_RS14185 at 13.845 kb on + strand, within EX31_RS14185 at 13.845 kb on + strand, within EX31_RS14185 at 13.846 kb on - strand, within EX31_RS14185 at 13.867 kb on + strand, within EX31_RS14185 at 13.868 kb on - strand, within EX31_RS14185 at 14.041 kb on + strand, within EX31_RS14185 at 14.042 kb on - strand, within EX31_RS14185 at 14.166 kb on + strand, within EX31_RS14185 at 14.166 kb on + strand, within EX31_RS14185 at 14.166 kb on + strand, within EX31_RS14185 at 14.167 kb on - strand, within EX31_RS14185 at 14.178 kb on + strand, within EX31_RS14185 at 14.178 kb on + strand, within EX31_RS14185 at 14.178 kb on + strand, within EX31_RS14185 at 14.240 kb on + strand at 14.240 kb on + strand at 14.240 kb on + strand at 14.241 kb on - strand at 14.247 kb on - strand at 14.263 kb on + strand at 14.263 kb on + strand at 14.263 kb on + strand at 14.264 kb on - strand at 14.264 kb on - strand at 14.264 kb on - strand at 14.264 kb on - strand at 14.264 kb on - strand at 14.264 kb on - strand at 14.295 kb on + strand at 14.296 kb on - strand at 14.299 kb on + strand at 14.299 kb on + strand at 14.299 kb on + strand at 14.300 kb on - strand at 14.300 kb on - strand at 14.302 kb on + strand at 14.302 kb on + strand at 14.303 kb on - strand at 14.303 kb on - strand at 14.308 kb on + strand at 14.308 kb on + strand at 14.308 kb on + strand at 14.309 kb on - strand at 14.309 kb on - strand at 14.309 kb on - strand at 14.309 kb on - strand at 14.310 kb on + strand at 14.310 kb on + strand at 14.310 kb on + strand at 14.311 kb on - strand at 14.311 kb on - strand at 14.311 kb on - strand at 14.311 kb on - strand at 14.314 kb on + strand at 14.314 kb on + strand at 14.314 kb on + strand at 14.319 kb on + strand at 14.319 kb on + strand at 14.320 kb on - strand at 14.324 kb on + strand at 14.325 kb on - strand at 14.325 kb on - strand at 14.325 kb on - strand at 14.325 kb on - strand at 14.325 kb on - strand at 14.325 kb on - strand at 14.325 kb on - strand at 14.346 kb on - strand at 14.469 kb on + strand at 14.470 kb on - strand at 14.478 kb on + strand at 14.582 kb on + strand, within EX31_RS14190 at 14.583 kb on - strand, within EX31_RS14190 at 14.610 kb on - strand, within EX31_RS14190 at 14.616 kb on + strand, within EX31_RS14190 at 14.617 kb on - strand, within EX31_RS14190 at 14.674 kb on - strand, within EX31_RS14190 at 14.674 kb on - strand, within EX31_RS14190 at 14.674 kb on - strand, within EX31_RS14190 at 14.674 kb on - strand, within EX31_RS14190 at 14.781 kb on - strand, within EX31_RS14190 at 14.781 kb on - strand, within EX31_RS14190 at 14.781 kb on - strand, within EX31_RS14190 at 14.841 kb on + strand, within EX31_RS14190 at 14.911 kb on - strand, within EX31_RS14190 at 14.911 kb on - strand, within EX31_RS14190 at 14.971 kb on + strand, within EX31_RS14190 at 14.972 kb on - strand, within EX31_RS14190 at 14.972 kb on - strand, within EX31_RS14190 at 14.972 kb on - strand, within EX31_RS14190 at 15.002 kb on + strand, within EX31_RS14190 at 15.048 kb on - strand, within EX31_RS14190 at 15.059 kb on + strand, within EX31_RS14190 at 15.059 kb on + strand, within EX31_RS14190 at 15.059 kb on + strand, within EX31_RS14190 at 15.059 kb on + strand, within EX31_RS14190 at 15.060 kb on - strand, within EX31_RS14190 at 15.060 kb on - strand, within EX31_RS14190 at 15.060 kb on - strand, within EX31_RS14190 at 15.094 kb on + strand, within EX31_RS14190 at 15.094 kb on + strand, within EX31_RS14190 at 15.095 kb on - strand, within EX31_RS14190 at 15.095 kb on - strand, within EX31_RS14190 at 15.095 kb on - strand, within EX31_RS14190 at 15.106 kb on + strand, within EX31_RS14190 at 15.107 kb on - strand, within EX31_RS14190 at 15.173 kb on - strand, within EX31_RS14190 at 15.173 kb on - strand, within EX31_RS14190 at 15.245 kb on - strand, within EX31_RS14190
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 12,510 + EX31_RS14180 0.35 -2.6 12,510 + EX31_RS14180 0.35 -2.5 12,511 - EX31_RS14180 0.35 -2.5 12,511 - EX31_RS14180 0.35 -2.5 12,629 + EX31_RS14180 0.43 -3.4 12,629 + EX31_RS14180 0.43 -4.7 12,671 + EX31_RS14180 0.46 -2.7 12,671 + EX31_RS14180 0.46 -3.8 12,671 + EX31_RS14180 0.46 -5.1 12,671 + EX31_RS14180 0.46 -2.8 12,672 - EX31_RS14180 0.47 -3.0 12,672 - EX31_RS14180 0.47 -2.5 12,672 - EX31_RS14180 0.47 -2.3 12,672 - EX31_RS14180 0.47 -2.5 12,738 - EX31_RS14180 0.51 -3.3 12,892 + EX31_RS14180 0.63 -2.8 12,892 + EX31_RS14180 0.63 -3.1 12,892 + EX31_RS14180 0.63 -4.2 12,892 + EX31_RS14180 0.63 -3.7 12,892 + EX31_RS14180 0.63 -2.1 12,892 + EX31_RS14180 0.63 -2.5 12,892 + EX31_RS14180 0.63 -2.8 12,892 + EX31_RS14180 0.63 -1.3 12,893 - EX31_RS14180 0.63 -4.3 12,893 - EX31_RS14180 0.63 -2.8 12,893 - EX31_RS14180 0.63 -2.0 13,002 + EX31_RS14180 0.71 -3.0 13,002 + EX31_RS14180 0.71 -1.9 13,003 - EX31_RS14180 0.71 -2.7 13,043 + EX31_RS14180 0.74 -4.1 13,043 + EX31_RS14180 0.74 -3.4 13,044 - EX31_RS14180 0.74 -4.0 13,044 - EX31_RS14180 0.74 +0.1 13,044 - EX31_RS14180 0.74 -2.5 13,044 - EX31_RS14180 0.74 -4.4 13,137 - EX31_RS14180 0.80 -1.5 13,151 + EX31_RS14180 0.81 -3.3 13,151 + EX31_RS14180 0.81 +0.8 13,151 + EX31_RS14180 0.81 -3.2 13,157 + EX31_RS14180 0.82 -3.3 13,158 - EX31_RS14180 0.82 -2.5 13,158 - EX31_RS14180 0.82 -5.1 13,158 - EX31_RS14180 0.82 -0.6 13,158 - EX31_RS14180 0.82 -4.5 13,176 + EX31_RS14180 0.83 -2.6 13,176 + EX31_RS14180 0.83 -3.7 13,177 - EX31_RS14180 0.83 -1.6 13,260 + EX31_RS14180 0.89 -3.3 13,260 + EX31_RS14180 0.89 -2.0 13,260 + EX31_RS14180 0.89 -5.8 13,261 - EX31_RS14180 0.89 -3.5 13,261 - EX31_RS14180 0.89 -3.7 13,261 - EX31_RS14180 0.89 -4.3 13,261 - EX31_RS14180 0.89 -5.0 13,261 - EX31_RS14180 0.89 -2.2 13,293 + -2.9 13,293 + -2.2 13,293 + -2.2 13,383 - -3.6 13,427 + -3.2 13,444 + -4.1 13,444 + -3.5 13,445 - -4.4 13,445 - -0.8 13,445 - +0.1 13,445 - +0.1 13,445 - -0.7 13,445 - -0.8 13,445 - -0.7 13,466 + -2.6 13,466 + +1.4 13,491 + -3.5 13,501 + -3.5 13,501 + -3.3 13,501 + -2.5 13,501 + -2.6 13,501 + -1.8 13,501 + -2.9 13,501 + -4.0 13,501 + -3.4 13,502 - -3.8 13,502 - -3.4 13,502 - -4.0 13,502 - -1.9 13,502 - -2.2 13,502 - -2.2 13,502 - -3.4 13,502 - -3.1 13,567 + -4.4 13,567 + -2.2 13,568 - EX31_RS14185 0.10 -3.1 13,639 + EX31_RS14185 0.19 -3.1 13,640 - EX31_RS14185 0.19 -1.7 13,640 - EX31_RS14185 0.19 -3.7 13,640 - EX31_RS14185 0.19 -2.7 13,642 + EX31_RS14185 0.20 -2.1 13,642 + EX31_RS14185 0.20 -2.5 13,642 + EX31_RS14185 0.20 -2.2 13,642 + EX31_RS14185 0.20 -2.5 13,642 + EX31_RS14185 0.20 -2.5 13,690 + EX31_RS14185 0.26 -3.9 13,690 + EX31_RS14185 0.26 -4.4 13,690 + EX31_RS14185 0.26 -1.7 13,845 + EX31_RS14185 0.46 -2.5 13,845 + EX31_RS14185 0.46 -3.2 13,846 - EX31_RS14185 0.46 -3.1 13,867 + EX31_RS14185 0.49 -3.0 13,868 - EX31_RS14185 0.49 -3.1 14,041 + EX31_RS14185 0.71 -2.3 14,042 - EX31_RS14185 0.71 -4.0 14,166 + EX31_RS14185 0.87 -0.1 14,166 + EX31_RS14185 0.87 -2.5 14,166 + EX31_RS14185 0.87 -2.3 14,167 - EX31_RS14185 0.87 -3.5 14,178 + EX31_RS14185 0.89 -2.3 14,178 + EX31_RS14185 0.89 -4.4 14,178 + EX31_RS14185 0.89 -1.2 14,240 + -4.9 14,240 + -3.3 14,240 + -3.1 14,241 - -3.0 14,247 - -4.5 14,263 + -2.9 14,263 + -4.0 14,263 + -2.1 14,264 - -3.5 14,264 - -3.2 14,264 - -4.2 14,264 - -3.5 14,264 - -2.1 14,264 - -4.0 14,295 + -4.1 14,296 - -1.2 14,299 + -1.3 14,299 + -1.1 14,299 + -0.6 14,300 - +0.1 14,300 - -0.2 14,302 + -4.1 14,302 + -2.9 14,303 - -0.1 14,303 - +0.1 14,308 + -1.9 14,308 + -3.7 14,308 + -2.9 14,309 - -0.1 14,309 - -0.5 14,309 - +0.5 14,309 - +0.1 14,310 + -2.2 14,310 + -2.8 14,310 + -2.4 14,311 - -0.0 14,311 - +0.1 14,311 - -1.4 14,311 - -0.9 14,314 + -2.3 14,314 + -3.5 14,314 + -3.7 14,319 + -3.6 14,319 + -1.8 14,320 - +0.9 14,324 + -4.7 14,325 - -0.4 14,325 - -0.7 14,325 - -0.3 14,325 - -0.5 14,325 - -1.2 14,325 - +0.5 14,325 - +2.8 14,346 - -0.0 14,469 + -2.8 14,470 - -3.8 14,478 + -3.1 14,582 + EX31_RS14190 0.19 -3.6 14,583 - EX31_RS14190 0.19 -4.4 14,610 - EX31_RS14190 0.21 -3.6 14,616 + EX31_RS14190 0.22 -5.2 14,617 - EX31_RS14190 0.22 -3.4 14,674 - EX31_RS14190 0.28 -3.5 14,674 - EX31_RS14190 0.28 -3.9 14,674 - EX31_RS14190 0.28 -4.2 14,674 - EX31_RS14190 0.28 -3.6 14,781 - EX31_RS14190 0.38 -2.2 14,781 - EX31_RS14190 0.38 -3.3 14,781 - EX31_RS14190 0.38 -2.4 14,841 + EX31_RS14190 0.44 -4.1 14,911 - EX31_RS14190 0.51 -1.7 14,911 - EX31_RS14190 0.51 -2.4 14,971 + EX31_RS14190 0.57 -2.0 14,972 - EX31_RS14190 0.57 -3.1 14,972 - EX31_RS14190 0.57 -4.2 14,972 - EX31_RS14190 0.57 -4.0 15,002 + EX31_RS14190 0.60 -2.5 15,048 - EX31_RS14190 0.65 -3.1 15,059 + EX31_RS14190 0.66 -1.6 15,059 + EX31_RS14190 0.66 -3.8 15,059 + EX31_RS14190 0.66 -4.6 15,059 + EX31_RS14190 0.66 -3.1 15,060 - EX31_RS14190 0.66 -0.9 15,060 - EX31_RS14190 0.66 -2.2 15,060 - EX31_RS14190 0.66 -4.1 15,094 + EX31_RS14190 0.70 -3.0 15,094 + EX31_RS14190 0.70 -3.4 15,095 - EX31_RS14190 0.70 -3.5 15,095 - EX31_RS14190 0.70 -3.1 15,095 - EX31_RS14190 0.70 -2.3 15,106 + EX31_RS14190 0.71 -2.1 15,107 - EX31_RS14190 0.71 -2.1 15,173 - EX31_RS14190 0.77 -1.5 15,173 - EX31_RS14190 0.77 -3.2 15,245 - EX31_RS14190 0.85 -3.0
Or see this region's nucleotide sequence