Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS12775 and EX31_RS12780 are separated by 55 nucleotides EX31_RS12780 and EX31_RS12785 are separated by 19 nucleotides EX31_RS12785 and EX31_RS12790 are separated by 248 nucleotides EX31_RS12790 and EX31_RS12795 are separated by 61 nucleotides
EX31_RS12775: EX31_RS12775 - 2-iminobutanoate/2-iminopropanoate deaminase, at 228,785 to 229,171
_RS12775
EX31_RS12780: EX31_RS12780 - aspartate carbamoyltransferase regulatory subunit, at 229,227 to 229,691
_RS12780
EX31_RS12785: EX31_RS12785 - aspartate carbamoyltransferase, at 229,711 to 230,646
_RS12785
EX31_RS12790: EX31_RS12790 - ArgR family transcriptional regulator, at 230,895 to 231,401
_RS12790
EX31_RS12795: EX31_RS12795 - YfcC family protein, at 231,463 to 232,866
_RS12795
Position (kb) 229
230
231 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 228.900 kb on + strand, within EX31_RS12775 at 228.900 kb on + strand, within EX31_RS12775 at 228.901 kb on - strand, within EX31_RS12775 at 228.969 kb on - strand, within EX31_RS12775 at 229.001 kb on + strand, within EX31_RS12775 at 229.001 kb on + strand, within EX31_RS12775 at 229.096 kb on + strand, within EX31_RS12775 at 229.096 kb on + strand, within EX31_RS12775 at 229.097 kb on - strand, within EX31_RS12775 at 229.097 kb on - strand, within EX31_RS12775 at 229.097 kb on - strand, within EX31_RS12775 at 229.122 kb on + strand, within EX31_RS12775 at 229.122 kb on + strand, within EX31_RS12775 at 229.122 kb on + strand, within EX31_RS12775 at 229.122 kb on + strand, within EX31_RS12775 at 229.123 kb on - strand, within EX31_RS12775 at 229.123 kb on - strand, within EX31_RS12775 at 229.123 kb on - strand, within EX31_RS12775 at 229.123 kb on - strand, within EX31_RS12775 at 229.171 kb on + strand at 229.171 kb on + strand at 229.171 kb on + strand at 229.171 kb on + strand at 229.171 kb on + strand at 229.171 kb on + strand at 229.171 kb on + strand at 229.171 kb on + strand at 229.171 kb on + strand at 229.171 kb on + strand at 229.171 kb on + strand at 229.171 kb on + strand at 229.172 kb on - strand at 229.172 kb on - strand at 229.172 kb on - strand at 229.172 kb on - strand at 229.172 kb on - strand at 229.172 kb on - strand at 229.172 kb on - strand at 229.172 kb on - strand at 229.172 kb on - strand at 229.172 kb on - strand at 229.218 kb on + strand at 229.219 kb on - strand at 229.219 kb on - strand at 229.220 kb on + strand at 229.220 kb on + strand at 229.236 kb on - strand at 229.273 kb on + strand at 229.273 kb on + strand at 229.273 kb on + strand at 229.426 kb on + strand, within EX31_RS12780 at 229.427 kb on - strand, within EX31_RS12780 at 229.427 kb on - strand, within EX31_RS12780 at 229.462 kb on + strand, within EX31_RS12780 at 229.462 kb on + strand, within EX31_RS12780 at 229.462 kb on + strand, within EX31_RS12780 at 229.463 kb on - strand, within EX31_RS12780 at 229.587 kb on - strand, within EX31_RS12780 at 229.631 kb on + strand, within EX31_RS12780 at 229.632 kb on - strand, within EX31_RS12780 at 229.646 kb on + strand at 229.712 kb on + strand at 229.713 kb on - strand at 229.713 kb on - strand at 229.713 kb on - strand at 229.790 kb on + strand at 229.800 kb on - strand at 229.800 kb on - strand at 229.809 kb on + strand, within EX31_RS12785 at 229.809 kb on + strand, within EX31_RS12785 at 229.810 kb on - strand, within EX31_RS12785 at 229.810 kb on - strand, within EX31_RS12785 at 229.926 kb on - strand, within EX31_RS12785 at 229.967 kb on + strand, within EX31_RS12785 at 229.967 kb on + strand, within EX31_RS12785 at 229.967 kb on + strand, within EX31_RS12785 at 229.968 kb on - strand, within EX31_RS12785 at 229.968 kb on - strand, within EX31_RS12785 at 230.018 kb on + strand, within EX31_RS12785 at 230.052 kb on + strand, within EX31_RS12785 at 230.052 kb on + strand, within EX31_RS12785 at 230.053 kb on - strand, within EX31_RS12785 at 230.054 kb on + strand, within EX31_RS12785 at 230.054 kb on + strand, within EX31_RS12785 at 230.054 kb on + strand, within EX31_RS12785 at 230.054 kb on + strand, within EX31_RS12785 at 230.054 kb on + strand, within EX31_RS12785 at 230.055 kb on - strand, within EX31_RS12785 at 230.086 kb on - strand, within EX31_RS12785 at 230.090 kb on + strand, within EX31_RS12785 at 230.090 kb on + strand, within EX31_RS12785 at 230.090 kb on + strand, within EX31_RS12785 at 230.091 kb on - strand, within EX31_RS12785 at 230.091 kb on - strand, within EX31_RS12785 at 230.091 kb on - strand, within EX31_RS12785 at 230.091 kb on - strand, within EX31_RS12785 at 230.091 kb on - strand, within EX31_RS12785 at 230.107 kb on - strand, within EX31_RS12785 at 230.107 kb on - strand, within EX31_RS12785 at 230.137 kb on + strand, within EX31_RS12785 at 230.138 kb on - strand, within EX31_RS12785 at 230.138 kb on - strand, within EX31_RS12785 at 230.138 kb on - strand, within EX31_RS12785 at 230.150 kb on + strand, within EX31_RS12785 at 230.178 kb on + strand, within EX31_RS12785 at 230.179 kb on - strand, within EX31_RS12785 at 230.179 kb on - strand, within EX31_RS12785 at 230.351 kb on + strand, within EX31_RS12785 at 230.351 kb on + strand, within EX31_RS12785 at 230.351 kb on + strand, within EX31_RS12785 at 230.351 kb on + strand, within EX31_RS12785 at 230.351 kb on + strand, within EX31_RS12785 at 230.351 kb on + strand, within EX31_RS12785 at 230.351 kb on + strand, within EX31_RS12785 at 230.352 kb on - strand, within EX31_RS12785 at 230.352 kb on - strand, within EX31_RS12785 at 230.352 kb on - strand, within EX31_RS12785 at 230.352 kb on - strand, within EX31_RS12785 at 230.352 kb on - strand, within EX31_RS12785 at 230.352 kb on - strand, within EX31_RS12785 at 230.352 kb on - strand, within EX31_RS12785 at 230.352 kb on - strand, within EX31_RS12785 at 230.352 kb on - strand, within EX31_RS12785 at 230.352 kb on - strand, within EX31_RS12785 at 230.611 kb on - strand at 230.668 kb on + strand at 230.675 kb on - strand at 230.744 kb on + strand at 230.744 kb on + strand at 230.745 kb on - strand at 230.745 kb on - strand at 230.856 kb on + strand at 230.859 kb on - strand at 230.859 kb on - strand at 230.859 kb on - strand at 230.859 kb on - strand at 231.005 kb on + strand, within EX31_RS12790 at 231.054 kb on - strand, within EX31_RS12790 at 231.093 kb on + strand, within EX31_RS12790 at 231.093 kb on + strand, within EX31_RS12790 at 231.093 kb on + strand, within EX31_RS12790 at 231.093 kb on + strand, within EX31_RS12790 at 231.094 kb on - strand, within EX31_RS12790 at 231.094 kb on - strand, within EX31_RS12790 at 231.094 kb on - strand, within EX31_RS12790 at 231.094 kb on - strand, within EX31_RS12790 at 231.094 kb on - strand, within EX31_RS12790 at 231.094 kb on - strand, within EX31_RS12790 at 231.094 kb on - strand, within EX31_RS12790 at 231.094 kb on - strand, within EX31_RS12790 at 231.094 kb on - strand, within EX31_RS12790 at 231.094 kb on - strand, within EX31_RS12790 at 231.094 kb on - strand, within EX31_RS12790 at 231.191 kb on - strand, within EX31_RS12790 at 231.191 kb on - strand, within EX31_RS12790 at 231.216 kb on + strand, within EX31_RS12790 at 231.216 kb on + strand, within EX31_RS12790 at 231.216 kb on + strand, within EX31_RS12790 at 231.217 kb on - strand, within EX31_RS12790 at 231.217 kb on - strand, within EX31_RS12790 at 231.338 kb on + strand, within EX31_RS12790 at 231.383 kb on + strand at 231.383 kb on + strand at 231.383 kb on + strand at 231.384 kb on - strand at 231.405 kb on + strand at 231.405 kb on + strand at 231.406 kb on - strand at 231.406 kb on - strand at 231.420 kb on + strand at 231.420 kb on + strand at 231.421 kb on - strand at 231.421 kb on - strand at 231.484 kb on + strand at 231.484 kb on + strand at 231.509 kb on - strand at 231.551 kb on + strand at 231.551 kb on + strand at 231.551 kb on + strand at 231.552 kb on - strand at 231.552 kb on - strand at 231.635 kb on + strand, within EX31_RS12795 at 231.636 kb on - strand, within EX31_RS12795
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 228,900 + EX31_RS12775 0.30 +0.7 228,900 + EX31_RS12775 0.30 +1.2 228,901 - EX31_RS12775 0.30 +0.9 228,969 - EX31_RS12775 0.48 +1.1 229,001 + EX31_RS12775 0.56 +0.1 229,001 + EX31_RS12775 0.56 -1.1 229,096 + EX31_RS12775 0.80 +0.2 229,096 + EX31_RS12775 0.80 -0.2 229,097 - EX31_RS12775 0.81 -0.0 229,097 - EX31_RS12775 0.81 -0.6 229,097 - EX31_RS12775 0.81 -0.4 229,122 + EX31_RS12775 0.87 +0.4 229,122 + EX31_RS12775 0.87 -0.1 229,122 + EX31_RS12775 0.87 -0.4 229,122 + EX31_RS12775 0.87 +0.5 229,123 - EX31_RS12775 0.87 -0.6 229,123 - EX31_RS12775 0.87 +0.1 229,123 - EX31_RS12775 0.87 -0.2 229,123 - EX31_RS12775 0.87 +2.1 229,171 + +0.1 229,171 + +0.6 229,171 + +0.0 229,171 + +0.0 229,171 + +1.0 229,171 + -1.3 229,171 + +1.1 229,171 + -1.2 229,171 + +0.6 229,171 + +0.2 229,171 + -0.1 229,171 + +0.0 229,172 - -0.9 229,172 - -0.8 229,172 - -0.3 229,172 - +1.2 229,172 - -0.0 229,172 - +0.7 229,172 - +0.1 229,172 - -0.7 229,172 - -0.6 229,172 - +0.0 229,218 + -1.4 229,219 - -0.8 229,219 - -0.6 229,220 + -0.5 229,220 + +0.6 229,236 - -0.0 229,273 + -0.7 229,273 + +0.8 229,273 + +0.2 229,426 + EX31_RS12780 0.43 +0.5 229,427 - EX31_RS12780 0.43 -0.5 229,427 - EX31_RS12780 0.43 +0.6 229,462 + EX31_RS12780 0.51 -0.5 229,462 + EX31_RS12780 0.51 +0.8 229,462 + EX31_RS12780 0.51 +0.3 229,463 - EX31_RS12780 0.51 +0.6 229,587 - EX31_RS12780 0.77 -0.9 229,631 + EX31_RS12780 0.87 +0.3 229,632 - EX31_RS12780 0.87 -0.3 229,646 + -0.0 229,712 + +0.2 229,713 - -0.1 229,713 - -0.9 229,713 - +0.0 229,790 + +0.6 229,800 - -3.1 229,800 - -2.5 229,809 + EX31_RS12785 0.10 -3.1 229,809 + EX31_RS12785 0.10 -3.7 229,810 - EX31_RS12785 0.11 -3.9 229,810 - EX31_RS12785 0.11 -2.2 229,926 - EX31_RS12785 0.23 -1.6 229,967 + EX31_RS12785 0.27 -2.8 229,967 + EX31_RS12785 0.27 -1.5 229,967 + EX31_RS12785 0.27 -4.0 229,968 - EX31_RS12785 0.27 -2.6 229,968 - EX31_RS12785 0.27 -0.1 230,018 + EX31_RS12785 0.33 -3.2 230,052 + EX31_RS12785 0.36 -2.7 230,052 + EX31_RS12785 0.36 -2.3 230,053 - EX31_RS12785 0.37 -3.1 230,054 + EX31_RS12785 0.37 -2.7 230,054 + EX31_RS12785 0.37 -2.8 230,054 + EX31_RS12785 0.37 -1.4 230,054 + EX31_RS12785 0.37 -0.6 230,054 + EX31_RS12785 0.37 -3.3 230,055 - EX31_RS12785 0.37 -4.1 230,086 - EX31_RS12785 0.40 -2.2 230,090 + EX31_RS12785 0.40 -2.9 230,090 + EX31_RS12785 0.40 -2.0 230,090 + EX31_RS12785 0.40 -2.7 230,091 - EX31_RS12785 0.41 -2.6 230,091 - EX31_RS12785 0.41 -2.8 230,091 - EX31_RS12785 0.41 -1.2 230,091 - EX31_RS12785 0.41 -3.6 230,091 - EX31_RS12785 0.41 -0.7 230,107 - EX31_RS12785 0.42 -1.5 230,107 - EX31_RS12785 0.42 -3.2 230,137 + EX31_RS12785 0.46 -3.5 230,138 - EX31_RS12785 0.46 -2.7 230,138 - EX31_RS12785 0.46 -1.9 230,138 - EX31_RS12785 0.46 -4.3 230,150 + EX31_RS12785 0.47 -4.0 230,178 + EX31_RS12785 0.50 -0.9 230,179 - EX31_RS12785 0.50 -4.2 230,179 - EX31_RS12785 0.50 -2.9 230,351 + EX31_RS12785 0.68 -4.1 230,351 + EX31_RS12785 0.68 -2.9 230,351 + EX31_RS12785 0.68 -4.9 230,351 + EX31_RS12785 0.68 -3.6 230,351 + EX31_RS12785 0.68 -4.2 230,351 + EX31_RS12785 0.68 -3.0 230,351 + EX31_RS12785 0.68 -2.4 230,352 - EX31_RS12785 0.68 -4.7 230,352 - EX31_RS12785 0.68 -4.3 230,352 - EX31_RS12785 0.68 -3.8 230,352 - EX31_RS12785 0.68 -1.9 230,352 - EX31_RS12785 0.68 -4.2 230,352 - EX31_RS12785 0.68 -3.3 230,352 - EX31_RS12785 0.68 +0.2 230,352 - EX31_RS12785 0.68 -3.8 230,352 - EX31_RS12785 0.68 -1.6 230,352 - EX31_RS12785 0.68 -3.7 230,611 - -3.2 230,668 + -0.6 230,675 - +0.6 230,744 + +0.6 230,744 + -0.8 230,745 - +2.2 230,745 - -0.1 230,856 + -1.7 230,859 - +0.9 230,859 - -0.9 230,859 - +0.3 230,859 - +1.1 231,005 + EX31_RS12790 0.22 -0.9 231,054 - EX31_RS12790 0.31 -0.6 231,093 + EX31_RS12790 0.39 +0.2 231,093 + EX31_RS12790 0.39 +0.1 231,093 + EX31_RS12790 0.39 +0.5 231,093 + EX31_RS12790 0.39 +0.8 231,094 - EX31_RS12790 0.39 +0.5 231,094 - EX31_RS12790 0.39 +0.1 231,094 - EX31_RS12790 0.39 -0.6 231,094 - EX31_RS12790 0.39 -0.6 231,094 - EX31_RS12790 0.39 -0.7 231,094 - EX31_RS12790 0.39 +0.2 231,094 - EX31_RS12790 0.39 +0.2 231,094 - EX31_RS12790 0.39 +0.8 231,094 - EX31_RS12790 0.39 +0.4 231,094 - EX31_RS12790 0.39 -0.5 231,094 - EX31_RS12790 0.39 +0.5 231,191 - EX31_RS12790 0.58 -1.1 231,191 - EX31_RS12790 0.58 +0.9 231,216 + EX31_RS12790 0.63 -1.1 231,216 + EX31_RS12790 0.63 +1.1 231,216 + EX31_RS12790 0.63 -0.5 231,217 - EX31_RS12790 0.64 +1.0 231,217 - EX31_RS12790 0.64 +0.4 231,338 + EX31_RS12790 0.87 +1.1 231,383 + +2.1 231,383 + +0.4 231,383 + +0.6 231,384 - +0.2 231,405 + -1.0 231,405 + -0.6 231,406 - +0.5 231,406 - -0.5 231,420 + -0.7 231,420 + +0.8 231,421 - -0.3 231,421 - -1.8 231,484 + +0.4 231,484 + +1.0 231,509 - +0.6 231,551 + -0.3 231,551 + +0.4 231,551 + -1.5 231,552 - +0.5 231,552 - +0.6 231,635 + EX31_RS12795 0.12 -0.5 231,636 - EX31_RS12795 0.12 -0.6
Or see this region's nucleotide sequence