Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS11535 and EX31_RS11540 are separated by 43 nucleotides EX31_RS11540 and EX31_RS11545 are separated by 37 nucleotides EX31_RS11545 and EX31_RS11550 are separated by 146 nucleotides
EX31_RS11535: EX31_RS11535 - outer membrane assembly protein AsmA, at 245,218 to 247,068
_RS11535
EX31_RS11540: EX31_RS11540 - dCTP deaminase, at 247,112 to 247,693
_RS11540
EX31_RS11545: EX31_RS11545 - uridine kinase, at 247,731 to 248,372
_RS11545
EX31_RS11550: EX31_RS11550 - iron-sulfur cluster carrier protein ApbC, at 248,519 to 249,631
_RS11550
Position (kb) 247
248
249 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 246.786 kb on + strand, within EX31_RS11535 at 246.786 kb on + strand, within EX31_RS11535 at 246.787 kb on - strand, within EX31_RS11535 at 246.787 kb on - strand, within EX31_RS11535 at 246.843 kb on + strand, within EX31_RS11535 at 246.962 kb on - strand at 246.968 kb on + strand at 246.969 kb on - strand at 246.969 kb on - strand at 246.969 kb on - strand at 246.969 kb on - strand at 246.969 kb on - strand at 246.969 kb on - strand at 246.969 kb on - strand at 246.969 kb on - strand at 247.001 kb on - strand at 247.001 kb on - strand at 247.037 kb on - strand at 247.127 kb on - strand at 247.182 kb on + strand, within EX31_RS11540 at 247.183 kb on - strand, within EX31_RS11540 at 247.183 kb on - strand, within EX31_RS11540 at 247.274 kb on - strand, within EX31_RS11540 at 247.274 kb on - strand, within EX31_RS11540 at 247.292 kb on - strand, within EX31_RS11540 at 247.541 kb on - strand, within EX31_RS11540 at 247.541 kb on - strand, within EX31_RS11540 at 247.543 kb on - strand, within EX31_RS11540 at 247.543 kb on - strand, within EX31_RS11540 at 247.596 kb on - strand, within EX31_RS11540 at 247.596 kb on - strand, within EX31_RS11540 at 247.596 kb on - strand, within EX31_RS11540 at 247.596 kb on - strand, within EX31_RS11540 at 247.644 kb on - strand at 247.670 kb on + strand at 247.671 kb on - strand at 247.671 kb on - strand at 247.671 kb on - strand at 247.671 kb on - strand at 247.671 kb on - strand at 247.671 kb on - strand at 247.671 kb on - strand at 247.671 kb on - strand at 247.671 kb on - strand at 247.673 kb on - strand at 247.697 kb on - strand at 247.733 kb on - strand at 247.733 kb on - strand at 247.817 kb on - strand, within EX31_RS11545 at 247.926 kb on - strand, within EX31_RS11545 at 247.943 kb on + strand, within EX31_RS11545 at 248.090 kb on - strand, within EX31_RS11545 at 248.090 kb on - strand, within EX31_RS11545 at 248.090 kb on - strand, within EX31_RS11545 at 248.090 kb on - strand, within EX31_RS11545 at 248.131 kb on - strand, within EX31_RS11545 at 248.131 kb on - strand, within EX31_RS11545 at 248.176 kb on + strand, within EX31_RS11545 at 248.177 kb on - strand, within EX31_RS11545 at 248.177 kb on - strand, within EX31_RS11545 at 248.177 kb on - strand, within EX31_RS11545 at 248.177 kb on - strand, within EX31_RS11545 at 248.177 kb on - strand, within EX31_RS11545 at 248.177 kb on - strand, within EX31_RS11545 at 248.177 kb on - strand, within EX31_RS11545 at 248.177 kb on - strand, within EX31_RS11545 at 248.223 kb on - strand, within EX31_RS11545 at 248.223 kb on - strand, within EX31_RS11545 at 248.224 kb on + strand, within EX31_RS11545 at 248.225 kb on - strand, within EX31_RS11545 at 248.225 kb on - strand, within EX31_RS11545 at 248.225 kb on - strand, within EX31_RS11545 at 248.225 kb on - strand, within EX31_RS11545 at 248.225 kb on - strand, within EX31_RS11545 at 248.225 kb on - strand, within EX31_RS11545 at 248.225 kb on - strand, within EX31_RS11545 at 248.287 kb on + strand, within EX31_RS11545 at 248.288 kb on - strand, within EX31_RS11545 at 248.288 kb on - strand, within EX31_RS11545 at 248.290 kb on - strand, within EX31_RS11545 at 248.290 kb on - strand, within EX31_RS11545 at 248.372 kb on + strand at 248.379 kb on - strand at 248.379 kb on - strand at 248.415 kb on + strand at 248.415 kb on + strand at 248.415 kb on + strand at 248.415 kb on + strand at 248.415 kb on + strand at 248.415 kb on + strand at 248.415 kb on + strand at 248.415 kb on + strand at 248.415 kb on + strand at 248.415 kb on + strand at 248.415 kb on + strand at 248.416 kb on - strand at 248.416 kb on - strand at 248.416 kb on - strand at 248.416 kb on - strand at 248.416 kb on - strand at 248.416 kb on - strand at 248.416 kb on - strand at 248.416 kb on - strand at 248.416 kb on - strand at 248.416 kb on - strand at 248.416 kb on - strand at 248.416 kb on - strand at 248.416 kb on - strand at 248.416 kb on - strand at 248.416 kb on - strand at 248.416 kb on - strand at 248.416 kb on - strand at 248.416 kb on - strand at 248.416 kb on - strand at 248.416 kb on - strand at 248.416 kb on - strand at 248.416 kb on - strand at 248.416 kb on - strand at 248.416 kb on - strand at 248.416 kb on - strand at 248.416 kb on - strand at 248.416 kb on - strand at 248.417 kb on + strand at 248.417 kb on + strand at 248.418 kb on - strand at 248.418 kb on - strand at 248.418 kb on - strand at 248.418 kb on - strand at 248.418 kb on - strand at 248.418 kb on - strand at 248.418 kb on - strand at 248.418 kb on - strand at 248.418 kb on - strand at 248.418 kb on - strand at 248.418 kb on - strand at 248.418 kb on - strand at 248.418 kb on - strand at 248.437 kb on + strand at 248.437 kb on + strand at 248.438 kb on - strand at 248.438 kb on - strand at 248.438 kb on - strand at 248.438 kb on - strand at 248.438 kb on - strand at 248.439 kb on + strand at 248.439 kb on + strand at 248.439 kb on + strand at 248.443 kb on + strand at 248.443 kb on + strand at 248.443 kb on + strand at 248.443 kb on + strand at 248.443 kb on + strand at 248.444 kb on - strand at 248.444 kb on - strand at 248.444 kb on - strand at 248.469 kb on + strand at 248.499 kb on + strand at 248.499 kb on + strand at 248.500 kb on - strand at 248.604 kb on + strand at 248.661 kb on - strand, within EX31_RS11550 at 248.661 kb on - strand, within EX31_RS11550 at 248.661 kb on - strand, within EX31_RS11550 at 248.680 kb on + strand, within EX31_RS11550 at 248.680 kb on + strand, within EX31_RS11550 at 248.791 kb on + strand, within EX31_RS11550 at 248.792 kb on - strand, within EX31_RS11550 at 248.792 kb on - strand, within EX31_RS11550 at 248.792 kb on - strand, within EX31_RS11550 at 248.792 kb on - strand, within EX31_RS11550 at 248.792 kb on - strand, within EX31_RS11550 at 248.804 kb on + strand, within EX31_RS11550 at 248.897 kb on + strand, within EX31_RS11550 at 248.942 kb on - strand, within EX31_RS11550 at 248.976 kb on + strand, within EX31_RS11550 at 248.999 kb on - strand, within EX31_RS11550 at 249.169 kb on + strand, within EX31_RS11550 at 249.169 kb on + strand, within EX31_RS11550 at 249.169 kb on + strand, within EX31_RS11550 at 249.190 kb on - strand, within EX31_RS11550 at 249.304 kb on + strand, within EX31_RS11550
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 246,786 + EX31_RS11535 0.85 +0.2 246,786 + EX31_RS11535 0.85 -1.8 246,787 - EX31_RS11535 0.85 +0.3 246,787 - EX31_RS11535 0.85 -1.6 246,843 + EX31_RS11535 0.88 +0.1 246,962 - -0.8 246,968 + +0.6 246,969 - -1.8 246,969 - +0.9 246,969 - +0.4 246,969 - -0.1 246,969 - +0.3 246,969 - -0.1 246,969 - +0.8 246,969 - +0.1 247,001 - -0.1 247,001 - +0.7 247,037 - +0.1 247,127 - -2.0 247,182 + EX31_RS11540 0.12 +1.2 247,183 - EX31_RS11540 0.12 -0.7 247,183 - EX31_RS11540 0.12 +0.3 247,274 - EX31_RS11540 0.28 -0.2 247,274 - EX31_RS11540 0.28 -0.7 247,292 - EX31_RS11540 0.31 -2.3 247,541 - EX31_RS11540 0.74 +0.5 247,541 - EX31_RS11540 0.74 -0.5 247,543 - EX31_RS11540 0.74 -0.9 247,543 - EX31_RS11540 0.74 -0.8 247,596 - EX31_RS11540 0.83 -1.3 247,596 - EX31_RS11540 0.83 -0.7 247,596 - EX31_RS11540 0.83 -0.9 247,596 - EX31_RS11540 0.83 -2.3 247,644 - +0.3 247,670 + +0.3 247,671 - -1.5 247,671 - -0.1 247,671 - +1.3 247,671 - -1.1 247,671 - -2.1 247,671 - -0.4 247,671 - -1.9 247,671 - +0.8 247,671 - -2.2 247,673 - -0.7 247,697 - -0.3 247,733 - +0.5 247,733 - -0.8 247,817 - EX31_RS11545 0.13 +0.7 247,926 - EX31_RS11545 0.30 -0.1 247,943 + EX31_RS11545 0.33 -0.5 248,090 - EX31_RS11545 0.56 +0.8 248,090 - EX31_RS11545 0.56 +1.1 248,090 - EX31_RS11545 0.56 -0.2 248,090 - EX31_RS11545 0.56 -0.7 248,131 - EX31_RS11545 0.62 -1.0 248,131 - EX31_RS11545 0.62 -2.2 248,176 + EX31_RS11545 0.69 +1.9 248,177 - EX31_RS11545 0.69 +3.5 248,177 - EX31_RS11545 0.69 -0.9 248,177 - EX31_RS11545 0.69 -0.5 248,177 - EX31_RS11545 0.69 +1.2 248,177 - EX31_RS11545 0.69 -1.4 248,177 - EX31_RS11545 0.69 -0.3 248,177 - EX31_RS11545 0.69 -0.7 248,177 - EX31_RS11545 0.69 +0.1 248,223 - EX31_RS11545 0.77 -1.2 248,223 - EX31_RS11545 0.77 -0.2 248,224 + EX31_RS11545 0.77 -0.4 248,225 - EX31_RS11545 0.77 -1.2 248,225 - EX31_RS11545 0.77 -0.5 248,225 - EX31_RS11545 0.77 +0.6 248,225 - EX31_RS11545 0.77 -0.6 248,225 - EX31_RS11545 0.77 -1.7 248,225 - EX31_RS11545 0.77 +0.5 248,225 - EX31_RS11545 0.77 -0.7 248,287 + EX31_RS11545 0.87 -1.5 248,288 - EX31_RS11545 0.87 +0.9 248,288 - EX31_RS11545 0.87 -0.4 248,290 - EX31_RS11545 0.87 -0.1 248,290 - EX31_RS11545 0.87 +0.4 248,372 + +0.1 248,379 - +0.3 248,379 - +0.3 248,415 + +0.9 248,415 + +0.9 248,415 + -0.1 248,415 + +1.6 248,415 + +1.2 248,415 + -0.5 248,415 + +0.3 248,415 + +0.2 248,415 + +1.9 248,415 + +0.9 248,415 + -0.7 248,416 - -0.5 248,416 - -0.9 248,416 - -0.4 248,416 - +1.0 248,416 - -0.3 248,416 - +0.5 248,416 - -0.2 248,416 - +0.6 248,416 - +0.3 248,416 - -0.1 248,416 - -0.0 248,416 - -0.1 248,416 - -1.4 248,416 - +0.6 248,416 - +0.5 248,416 - +0.5 248,416 - -0.2 248,416 - +0.7 248,416 - -1.6 248,416 - -0.1 248,416 - -1.3 248,416 - -0.4 248,416 - -0.7 248,416 - -1.1 248,416 - -0.4 248,416 - -1.1 248,416 - -0.3 248,417 + +0.2 248,417 + +0.3 248,418 - +0.9 248,418 - +0.3 248,418 - +0.4 248,418 - +0.2 248,418 - +0.7 248,418 - +0.5 248,418 - +1.1 248,418 - -0.1 248,418 - +0.5 248,418 - -1.4 248,418 - +0.7 248,418 - +0.5 248,418 - -1.9 248,437 + +0.9 248,437 + -0.9 248,438 - +1.1 248,438 - +0.9 248,438 - +0.3 248,438 - +0.4 248,438 - +0.6 248,439 + -1.5 248,439 + -1.2 248,439 + -1.2 248,443 + +1.7 248,443 + -1.5 248,443 + -1.7 248,443 + +1.7 248,443 + -1.2 248,444 - +1.2 248,444 - -0.4 248,444 - -0.5 248,469 + +0.6 248,499 + +0.2 248,499 + -0.1 248,500 - -0.0 248,604 + +1.0 248,661 - EX31_RS11550 0.13 +0.3 248,661 - EX31_RS11550 0.13 +0.9 248,661 - EX31_RS11550 0.13 +0.0 248,680 + EX31_RS11550 0.14 +1.8 248,680 + EX31_RS11550 0.14 +0.3 248,791 + EX31_RS11550 0.24 -0.6 248,792 - EX31_RS11550 0.25 +1.9 248,792 - EX31_RS11550 0.25 +0.4 248,792 - EX31_RS11550 0.25 +0.7 248,792 - EX31_RS11550 0.25 +0.4 248,792 - EX31_RS11550 0.25 -0.5 248,804 + EX31_RS11550 0.26 +0.7 248,897 + EX31_RS11550 0.34 -0.1 248,942 - EX31_RS11550 0.38 -0.5 248,976 + EX31_RS11550 0.41 -1.7 248,999 - EX31_RS11550 0.43 -0.1 249,169 + EX31_RS11550 0.58 -0.4 249,169 + EX31_RS11550 0.58 -0.1 249,169 + EX31_RS11550 0.58 +0.2 249,190 - EX31_RS11550 0.60 -0.1 249,304 + EX31_RS11550 0.71 -0.1
Or see this region's nucleotide sequence