Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS11445 and EX31_RS11450 overlap by 4 nucleotides EX31_RS11450 and EX31_RS11455 overlap by 1 nucleotides EX31_RS11455 and EX31_RS11460 are separated by 5 nucleotides
EX31_RS11445: EX31_RS11445 - histidinol-phosphate transaminase, at 223,334 to 224,467
_RS11445
EX31_RS11450: EX31_RS11450 - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 224,464 to 225,546
_RS11450
EX31_RS11455: EX31_RS11455 - imidazole glycerol phosphate synthase subunit HisH, at 225,546 to 226,136
_RS11455
EX31_RS11460: EX31_RS11460 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 226,142 to 226,879
_RS11460
Position (kb) 224
225
226 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 223.550 kb on + strand, within EX31_RS11445 at 223.550 kb on + strand, within EX31_RS11445 at 223.679 kb on + strand, within EX31_RS11445 at 223.679 kb on + strand, within EX31_RS11445 at 223.680 kb on - strand, within EX31_RS11445 at 223.680 kb on - strand, within EX31_RS11445 at 223.680 kb on - strand, within EX31_RS11445 at 223.680 kb on - strand, within EX31_RS11445 at 223.680 kb on - strand, within EX31_RS11445 at 223.793 kb on + strand, within EX31_RS11445 at 223.793 kb on + strand, within EX31_RS11445 at 223.825 kb on + strand, within EX31_RS11445 at 223.884 kb on + strand, within EX31_RS11445 at 223.901 kb on + strand, within EX31_RS11445 at 223.901 kb on + strand, within EX31_RS11445 at 223.901 kb on + strand, within EX31_RS11445 at 223.901 kb on + strand, within EX31_RS11445 at 223.901 kb on + strand, within EX31_RS11445 at 223.901 kb on + strand, within EX31_RS11445 at 223.901 kb on + strand, within EX31_RS11445 at 223.901 kb on + strand, within EX31_RS11445 at 223.902 kb on - strand, within EX31_RS11445 at 223.902 kb on - strand, within EX31_RS11445 at 223.902 kb on - strand, within EX31_RS11445 at 223.903 kb on + strand, within EX31_RS11445 at 223.904 kb on - strand, within EX31_RS11445 at 224.046 kb on + strand, within EX31_RS11445 at 224.046 kb on + strand, within EX31_RS11445 at 224.047 kb on - strand, within EX31_RS11445 at 224.050 kb on - strand, within EX31_RS11445 at 224.070 kb on - strand, within EX31_RS11445 at 224.076 kb on + strand, within EX31_RS11445 at 224.080 kb on + strand, within EX31_RS11445 at 224.081 kb on - strand, within EX31_RS11445 at 224.233 kb on + strand, within EX31_RS11445 at 224.233 kb on + strand, within EX31_RS11445 at 224.234 kb on - strand, within EX31_RS11445 at 224.234 kb on - strand, within EX31_RS11445 at 224.234 kb on - strand, within EX31_RS11445 at 224.237 kb on + strand, within EX31_RS11445 at 224.237 kb on + strand, within EX31_RS11445 at 224.237 kb on + strand, within EX31_RS11445 at 224.238 kb on - strand, within EX31_RS11445 at 224.238 kb on - strand, within EX31_RS11445 at 224.238 kb on - strand, within EX31_RS11445 at 224.255 kb on + strand, within EX31_RS11445 at 224.255 kb on + strand, within EX31_RS11445 at 224.276 kb on - strand, within EX31_RS11445 at 224.434 kb on + strand at 224.600 kb on + strand, within EX31_RS11450 at 224.601 kb on - strand, within EX31_RS11450 at 224.834 kb on + strand, within EX31_RS11450 at 224.834 kb on + strand, within EX31_RS11450 at 224.854 kb on + strand, within EX31_RS11450 at 224.854 kb on + strand, within EX31_RS11450 at 224.854 kb on + strand, within EX31_RS11450 at 224.854 kb on + strand, within EX31_RS11450 at 224.854 kb on + strand, within EX31_RS11450 at 224.854 kb on + strand, within EX31_RS11450 at 224.854 kb on + strand, within EX31_RS11450 at 224.855 kb on - strand, within EX31_RS11450 at 225.087 kb on + strand, within EX31_RS11450 at 225.087 kb on + strand, within EX31_RS11450 at 225.087 kb on + strand, within EX31_RS11450 at 225.087 kb on + strand, within EX31_RS11450 at 225.088 kb on - strand, within EX31_RS11450 at 225.151 kb on + strand, within EX31_RS11450 at 225.151 kb on + strand, within EX31_RS11450 at 225.151 kb on + strand, within EX31_RS11450 at 225.151 kb on + strand, within EX31_RS11450 at 225.151 kb on + strand, within EX31_RS11450 at 225.151 kb on + strand, within EX31_RS11450 at 225.151 kb on + strand, within EX31_RS11450 at 225.151 kb on + strand, within EX31_RS11450 at 225.152 kb on - strand, within EX31_RS11450 at 225.225 kb on + strand, within EX31_RS11450 at 225.320 kb on - strand, within EX31_RS11450 at 225.334 kb on - strand, within EX31_RS11450 at 225.337 kb on + strand, within EX31_RS11450 at 225.397 kb on + strand, within EX31_RS11450 at 225.397 kb on + strand, within EX31_RS11450 at 225.397 kb on + strand, within EX31_RS11450 at 225.398 kb on - strand, within EX31_RS11450 at 225.597 kb on + strand at 225.891 kb on - strand, within EX31_RS11455 at 225.891 kb on - strand, within EX31_RS11455 at 225.891 kb on - strand, within EX31_RS11455 at 225.932 kb on - strand, within EX31_RS11455 at 225.932 kb on - strand, within EX31_RS11455 at 225.957 kb on + strand, within EX31_RS11455 at 225.957 kb on + strand, within EX31_RS11455 at 225.957 kb on + strand, within EX31_RS11455 at 225.958 kb on - strand, within EX31_RS11455 at 225.958 kb on - strand, within EX31_RS11455 at 225.964 kb on - strand, within EX31_RS11455 at 225.964 kb on - strand, within EX31_RS11455 at 225.964 kb on - strand, within EX31_RS11455 at 226.020 kb on + strand, within EX31_RS11455 at 226.134 kb on + strand at 226.134 kb on + strand at 226.134 kb on + strand at 226.134 kb on + strand at 226.134 kb on + strand at 226.135 kb on - strand at 226.135 kb on - strand at 226.135 kb on - strand at 226.147 kb on + strand at 226.202 kb on + strand at 226.202 kb on + strand at 226.345 kb on - strand, within EX31_RS11460 at 226.530 kb on + strand, within EX31_RS11460 at 226.530 kb on + strand, within EX31_RS11460 at 226.531 kb on - strand, within EX31_RS11460
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 223,550 + EX31_RS11445 0.19 -2.0 223,550 + EX31_RS11445 0.19 -2.1 223,679 + EX31_RS11445 0.30 -2.9 223,679 + EX31_RS11445 0.30 -1.9 223,680 - EX31_RS11445 0.31 -1.4 223,680 - EX31_RS11445 0.31 +0.3 223,680 - EX31_RS11445 0.31 -3.4 223,680 - EX31_RS11445 0.31 +0.6 223,680 - EX31_RS11445 0.31 -2.0 223,793 + EX31_RS11445 0.40 -1.9 223,793 + EX31_RS11445 0.40 -1.9 223,825 + EX31_RS11445 0.43 -2.3 223,884 + EX31_RS11445 0.49 -1.1 223,901 + EX31_RS11445 0.50 -1.8 223,901 + EX31_RS11445 0.50 -1.1 223,901 + EX31_RS11445 0.50 -2.4 223,901 + EX31_RS11445 0.50 -2.8 223,901 + EX31_RS11445 0.50 -2.1 223,901 + EX31_RS11445 0.50 -0.7 223,901 + EX31_RS11445 0.50 -2.2 223,901 + EX31_RS11445 0.50 -3.7 223,902 - EX31_RS11445 0.50 -1.1 223,902 - EX31_RS11445 0.50 -1.4 223,902 - EX31_RS11445 0.50 -2.4 223,903 + EX31_RS11445 0.50 -2.4 223,904 - EX31_RS11445 0.50 -1.5 224,046 + EX31_RS11445 0.63 -2.5 224,046 + EX31_RS11445 0.63 -2.8 224,047 - EX31_RS11445 0.63 +0.3 224,050 - EX31_RS11445 0.63 -3.2 224,070 - EX31_RS11445 0.65 -2.3 224,076 + EX31_RS11445 0.65 -2.2 224,080 + EX31_RS11445 0.66 +0.9 224,081 - EX31_RS11445 0.66 -0.9 224,233 + EX31_RS11445 0.79 -2.9 224,233 + EX31_RS11445 0.79 -2.2 224,234 - EX31_RS11445 0.79 -1.2 224,234 - EX31_RS11445 0.79 -1.9 224,234 - EX31_RS11445 0.79 -2.0 224,237 + EX31_RS11445 0.80 -1.5 224,237 + EX31_RS11445 0.80 -0.9 224,237 + EX31_RS11445 0.80 +0.3 224,238 - EX31_RS11445 0.80 -0.7 224,238 - EX31_RS11445 0.80 -1.2 224,238 - EX31_RS11445 0.80 -1.4 224,255 + EX31_RS11445 0.81 -0.4 224,255 + EX31_RS11445 0.81 -1.7 224,276 - EX31_RS11445 0.83 -2.4 224,434 + +0.2 224,600 + EX31_RS11450 0.13 -3.0 224,601 - EX31_RS11450 0.13 -1.4 224,834 + EX31_RS11450 0.34 -0.4 224,834 + EX31_RS11450 0.34 -2.6 224,854 + EX31_RS11450 0.36 -0.9 224,854 + EX31_RS11450 0.36 -1.5 224,854 + EX31_RS11450 0.36 -0.9 224,854 + EX31_RS11450 0.36 -0.9 224,854 + EX31_RS11450 0.36 -1.8 224,854 + EX31_RS11450 0.36 -2.2 224,854 + EX31_RS11450 0.36 -0.7 224,855 - EX31_RS11450 0.36 -1.9 225,087 + EX31_RS11450 0.58 -0.4 225,087 + EX31_RS11450 0.58 -0.7 225,087 + EX31_RS11450 0.58 -1.8 225,087 + EX31_RS11450 0.58 +0.3 225,088 - EX31_RS11450 0.58 -2.6 225,151 + EX31_RS11450 0.63 -2.8 225,151 + EX31_RS11450 0.63 -1.1 225,151 + EX31_RS11450 0.63 -1.9 225,151 + EX31_RS11450 0.63 -2.8 225,151 + EX31_RS11450 0.63 -2.3 225,151 + EX31_RS11450 0.63 -3.3 225,151 + EX31_RS11450 0.63 -2.1 225,151 + EX31_RS11450 0.63 -0.1 225,152 - EX31_RS11450 0.64 -0.7 225,225 + EX31_RS11450 0.70 +0.3 225,320 - EX31_RS11450 0.79 -1.5 225,334 - EX31_RS11450 0.80 -2.3 225,337 + EX31_RS11450 0.81 -1.5 225,397 + EX31_RS11450 0.86 +0.3 225,397 + EX31_RS11450 0.86 -1.8 225,397 + EX31_RS11450 0.86 -2.6 225,398 - EX31_RS11450 0.86 -2.2 225,597 + +0.9 225,891 - EX31_RS11455 0.58 -2.5 225,891 - EX31_RS11455 0.58 -0.9 225,891 - EX31_RS11455 0.58 -0.7 225,932 - EX31_RS11455 0.65 -1.8 225,932 - EX31_RS11455 0.65 -0.5 225,957 + EX31_RS11455 0.70 -1.4 225,957 + EX31_RS11455 0.70 -2.5 225,957 + EX31_RS11455 0.70 -0.9 225,958 - EX31_RS11455 0.70 -3.2 225,958 - EX31_RS11455 0.70 -0.9 225,964 - EX31_RS11455 0.71 -2.0 225,964 - EX31_RS11455 0.71 -3.1 225,964 - EX31_RS11455 0.71 -2.3 226,020 + EX31_RS11455 0.80 -2.2 226,134 + -1.4 226,134 + -2.8 226,134 + -2.2 226,134 + -0.7 226,134 + -3.4 226,135 - -2.2 226,135 - -2.0 226,135 - -1.7 226,147 + -2.2 226,202 + -1.2 226,202 + -2.2 226,345 - EX31_RS11460 0.28 -2.0 226,530 + EX31_RS11460 0.53 -2.1 226,530 + EX31_RS11460 0.53 -0.1 226,531 - EX31_RS11460 0.53 -2.2
Or see this region's nucleotide sequence