Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS11405 and EX31_RS11410 are separated by 30 nucleotides EX31_RS11410 and EX31_RS11415 overlap by 4 nucleotides
EX31_RS11405: EX31_RS11405 - chemotaxis protein CheA, at 213,311 to 215,353
_RS11405
EX31_RS11410: EX31_RS11410 - flagellar motor protein MotB, at 215,384 to 216,487
_RS11410
EX31_RS11415: EX31_RS11415 - flagellar motor stator protein MotA, at 216,484 to 217,374
_RS11415
Position (kb) 215
216
217 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 214.712 kb on - strand, within EX31_RS11405 at 214.781 kb on + strand, within EX31_RS11405 at 215.000 kb on - strand, within EX31_RS11405 at 215.000 kb on - strand, within EX31_RS11405 at 215.000 kb on - strand, within EX31_RS11405 at 215.000 kb on - strand, within EX31_RS11405 at 215.000 kb on - strand, within EX31_RS11405 at 215.000 kb on - strand, within EX31_RS11405 at 215.002 kb on - strand, within EX31_RS11405 at 215.002 kb on - strand, within EX31_RS11405 at 215.002 kb on - strand, within EX31_RS11405 at 215.002 kb on - strand, within EX31_RS11405 at 215.038 kb on + strand, within EX31_RS11405 at 215.038 kb on + strand, within EX31_RS11405 at 215.062 kb on + strand, within EX31_RS11405 at 215.063 kb on - strand, within EX31_RS11405 at 215.151 kb on - strand at 215.169 kb on - strand at 215.267 kb on + strand at 215.267 kb on + strand at 215.268 kb on - strand at 215.577 kb on - strand, within EX31_RS11410 at 215.603 kb on + strand, within EX31_RS11410 at 215.603 kb on + strand, within EX31_RS11410 at 215.603 kb on + strand, within EX31_RS11410 at 215.603 kb on + strand, within EX31_RS11410 at 215.604 kb on - strand, within EX31_RS11410 at 215.604 kb on - strand, within EX31_RS11410 at 215.604 kb on - strand, within EX31_RS11410 at 215.604 kb on - strand, within EX31_RS11410 at 215.604 kb on - strand, within EX31_RS11410 at 215.604 kb on - strand, within EX31_RS11410 at 215.604 kb on - strand, within EX31_RS11410 at 215.710 kb on + strand, within EX31_RS11410 at 215.711 kb on - strand, within EX31_RS11410 at 215.711 kb on - strand, within EX31_RS11410 at 215.865 kb on + strand, within EX31_RS11410 at 215.887 kb on - strand, within EX31_RS11410 at 215.903 kb on - strand, within EX31_RS11410 at 215.976 kb on + strand, within EX31_RS11410 at 215.976 kb on + strand, within EX31_RS11410 at 215.976 kb on + strand, within EX31_RS11410 at 215.977 kb on - strand, within EX31_RS11410 at 215.977 kb on - strand, within EX31_RS11410 at 215.977 kb on - strand, within EX31_RS11410 at 215.977 kb on - strand, within EX31_RS11410 at 216.143 kb on + strand, within EX31_RS11410 at 216.144 kb on - strand, within EX31_RS11410 at 216.163 kb on + strand, within EX31_RS11410 at 216.164 kb on - strand, within EX31_RS11410 at 216.247 kb on + strand, within EX31_RS11410 at 216.304 kb on - strand, within EX31_RS11410 at 216.386 kb on - strand at 216.386 kb on - strand at 216.386 kb on - strand at 216.440 kb on - strand at 216.440 kb on - strand at 216.455 kb on + strand at 216.455 kb on + strand at 216.456 kb on - strand at 216.456 kb on - strand at 216.456 kb on - strand at 216.487 kb on + strand at 216.488 kb on - strand at 216.488 kb on - strand at 216.488 kb on - strand at 216.488 kb on - strand at 216.556 kb on - strand at 216.578 kb on + strand, within EX31_RS11415 at 216.578 kb on + strand, within EX31_RS11415 at 216.578 kb on + strand, within EX31_RS11415 at 216.578 kb on + strand, within EX31_RS11415 at 216.578 kb on + strand, within EX31_RS11415 at 216.578 kb on + strand, within EX31_RS11415 at 216.579 kb on - strand, within EX31_RS11415 at 216.579 kb on - strand, within EX31_RS11415 at 216.579 kb on - strand, within EX31_RS11415 at 216.579 kb on - strand, within EX31_RS11415 at 216.579 kb on - strand, within EX31_RS11415 at 216.579 kb on - strand, within EX31_RS11415 at 216.695 kb on - strand, within EX31_RS11415 at 216.816 kb on + strand, within EX31_RS11415 at 216.816 kb on + strand, within EX31_RS11415 at 216.817 kb on - strand, within EX31_RS11415 at 216.817 kb on - strand, within EX31_RS11415 at 216.820 kb on + strand, within EX31_RS11415 at 216.820 kb on + strand, within EX31_RS11415 at 216.820 kb on + strand, within EX31_RS11415 at 216.821 kb on - strand, within EX31_RS11415 at 216.821 kb on - strand, within EX31_RS11415 at 216.821 kb on - strand, within EX31_RS11415 at 216.821 kb on - strand, within EX31_RS11415 at 216.821 kb on - strand, within EX31_RS11415 at 216.821 kb on - strand, within EX31_RS11415 at 216.821 kb on - strand, within EX31_RS11415 at 216.821 kb on - strand, within EX31_RS11415 at 216.821 kb on - strand, within EX31_RS11415 at 216.821 kb on - strand, within EX31_RS11415 at 216.821 kb on - strand, within EX31_RS11415 at 216.821 kb on - strand, within EX31_RS11415 at 216.914 kb on + strand, within EX31_RS11415 at 216.915 kb on - strand, within EX31_RS11415 at 216.915 kb on - strand, within EX31_RS11415 at 216.915 kb on - strand, within EX31_RS11415 at 216.989 kb on + strand, within EX31_RS11415 at 217.149 kb on + strand, within EX31_RS11415 at 217.150 kb on - strand, within EX31_RS11415 at 217.150 kb on - strand, within EX31_RS11415 at 217.151 kb on + strand, within EX31_RS11415 at 217.151 kb on + strand, within EX31_RS11415 at 217.151 kb on + strand, within EX31_RS11415 at 217.151 kb on + strand, within EX31_RS11415 at 217.151 kb on + strand, within EX31_RS11415 at 217.151 kb on + strand, within EX31_RS11415 at 217.152 kb on - strand, within EX31_RS11415 at 217.152 kb on - strand, within EX31_RS11415 at 217.152 kb on - strand, within EX31_RS11415 at 217.152 kb on - strand, within EX31_RS11415 at 217.152 kb on - strand, within EX31_RS11415 at 217.152 kb on - strand, within EX31_RS11415 at 217.152 kb on - strand, within EX31_RS11415 at 217.152 kb on - strand, within EX31_RS11415 at 217.152 kb on - strand, within EX31_RS11415 at 217.152 kb on - strand, within EX31_RS11415 at 217.161 kb on + strand, within EX31_RS11415 at 217.161 kb on + strand, within EX31_RS11415 at 217.166 kb on + strand, within EX31_RS11415 at 217.289 kb on + strand at 217.289 kb on + strand at 217.290 kb on - strand at 217.290 kb on - strand at 217.290 kb on - strand at 217.290 kb on - strand at 217.290 kb on - strand at 217.290 kb on - strand at 217.290 kb on - strand at 217.290 kb on - strand at 217.355 kb on + strand at 217.355 kb on + strand at 217.355 kb on + strand at 217.356 kb on - strand at 217.360 kb on + strand at 217.361 kb on - strand at 217.361 kb on - strand at 217.361 kb on - strand at 217.364 kb on - strand at 217.386 kb on - strand at 217.386 kb on - strand at 217.460 kb on - strand at 217.460 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 214,712 - EX31_RS11405 0.69 -1.0 214,781 + EX31_RS11405 0.72 -0.5 215,000 - EX31_RS11405 0.83 -0.2 215,000 - EX31_RS11405 0.83 -0.9 215,000 - EX31_RS11405 0.83 +0.0 215,000 - EX31_RS11405 0.83 -0.2 215,000 - EX31_RS11405 0.83 -0.4 215,000 - EX31_RS11405 0.83 -1.1 215,002 - EX31_RS11405 0.83 +0.1 215,002 - EX31_RS11405 0.83 +0.2 215,002 - EX31_RS11405 0.83 -0.3 215,002 - EX31_RS11405 0.83 -1.5 215,038 + EX31_RS11405 0.85 -0.2 215,038 + EX31_RS11405 0.85 -1.2 215,062 + EX31_RS11405 0.86 +0.3 215,063 - EX31_RS11405 0.86 -1.7 215,151 - -0.3 215,169 - +0.6 215,267 + -0.2 215,267 + -0.4 215,268 - -1.0 215,577 - EX31_RS11410 0.17 +0.6 215,603 + EX31_RS11410 0.20 -0.9 215,603 + EX31_RS11410 0.20 -2.5 215,603 + EX31_RS11410 0.20 -0.8 215,603 + EX31_RS11410 0.20 -2.2 215,604 - EX31_RS11410 0.20 -0.2 215,604 - EX31_RS11410 0.20 +0.3 215,604 - EX31_RS11410 0.20 -0.7 215,604 - EX31_RS11410 0.20 +0.6 215,604 - EX31_RS11410 0.20 -2.0 215,604 - EX31_RS11410 0.20 -0.9 215,604 - EX31_RS11410 0.20 -0.8 215,710 + EX31_RS11410 0.30 -0.6 215,711 - EX31_RS11410 0.30 -0.9 215,711 - EX31_RS11410 0.30 -0.6 215,865 + EX31_RS11410 0.44 -0.7 215,887 - EX31_RS11410 0.46 -1.9 215,903 - EX31_RS11410 0.47 -3.2 215,976 + EX31_RS11410 0.54 -0.7 215,976 + EX31_RS11410 0.54 -1.1 215,976 + EX31_RS11410 0.54 +0.1 215,977 - EX31_RS11410 0.54 -2.8 215,977 - EX31_RS11410 0.54 -0.7 215,977 - EX31_RS11410 0.54 +0.3 215,977 - EX31_RS11410 0.54 -0.4 216,143 + EX31_RS11410 0.69 +0.1 216,144 - EX31_RS11410 0.69 +1.0 216,163 + EX31_RS11410 0.71 -2.2 216,164 - EX31_RS11410 0.71 -0.2 216,247 + EX31_RS11410 0.78 +0.2 216,304 - EX31_RS11410 0.83 -0.8 216,386 - -1.2 216,386 - -1.5 216,386 - -0.9 216,440 - -0.4 216,440 - +0.6 216,455 + +0.7 216,455 + +0.1 216,456 - -0.1 216,456 - -2.1 216,456 - +0.0 216,487 + +0.9 216,488 - -0.3 216,488 - -0.2 216,488 - -1.3 216,488 - -0.5 216,556 - -0.1 216,578 + EX31_RS11415 0.11 +0.4 216,578 + EX31_RS11415 0.11 -0.3 216,578 + EX31_RS11415 0.11 -0.1 216,578 + EX31_RS11415 0.11 +1.9 216,578 + EX31_RS11415 0.11 +2.9 216,578 + EX31_RS11415 0.11 -0.5 216,579 - EX31_RS11415 0.11 -0.2 216,579 - EX31_RS11415 0.11 +1.3 216,579 - EX31_RS11415 0.11 -0.7 216,579 - EX31_RS11415 0.11 -1.6 216,579 - EX31_RS11415 0.11 -0.1 216,579 - EX31_RS11415 0.11 +0.6 216,695 - EX31_RS11415 0.24 -1.4 216,816 + EX31_RS11415 0.37 -0.9 216,816 + EX31_RS11415 0.37 +0.5 216,817 - EX31_RS11415 0.37 -1.0 216,817 - EX31_RS11415 0.37 -0.8 216,820 + EX31_RS11415 0.38 -0.4 216,820 + EX31_RS11415 0.38 +0.9 216,820 + EX31_RS11415 0.38 +0.2 216,821 - EX31_RS11415 0.38 +1.9 216,821 - EX31_RS11415 0.38 +0.9 216,821 - EX31_RS11415 0.38 +0.1 216,821 - EX31_RS11415 0.38 -0.7 216,821 - EX31_RS11415 0.38 +1.1 216,821 - EX31_RS11415 0.38 -0.9 216,821 - EX31_RS11415 0.38 -2.2 216,821 - EX31_RS11415 0.38 -0.9 216,821 - EX31_RS11415 0.38 -0.2 216,821 - EX31_RS11415 0.38 -0.2 216,821 - EX31_RS11415 0.38 +1.1 216,821 - EX31_RS11415 0.38 +0.3 216,914 + EX31_RS11415 0.48 +0.9 216,915 - EX31_RS11415 0.48 +0.1 216,915 - EX31_RS11415 0.48 -0.3 216,915 - EX31_RS11415 0.48 -1.5 216,989 + EX31_RS11415 0.57 -0.3 217,149 + EX31_RS11415 0.75 +1.3 217,150 - EX31_RS11415 0.75 -1.2 217,150 - EX31_RS11415 0.75 -0.7 217,151 + EX31_RS11415 0.75 -0.4 217,151 + EX31_RS11415 0.75 -0.9 217,151 + EX31_RS11415 0.75 -1.1 217,151 + EX31_RS11415 0.75 +0.2 217,151 + EX31_RS11415 0.75 -0.9 217,151 + EX31_RS11415 0.75 -0.4 217,152 - EX31_RS11415 0.75 -1.2 217,152 - EX31_RS11415 0.75 -1.1 217,152 - EX31_RS11415 0.75 -0.4 217,152 - EX31_RS11415 0.75 +0.7 217,152 - EX31_RS11415 0.75 -0.1 217,152 - EX31_RS11415 0.75 -1.9 217,152 - EX31_RS11415 0.75 -2.5 217,152 - EX31_RS11415 0.75 -0.8 217,152 - EX31_RS11415 0.75 -0.7 217,152 - EX31_RS11415 0.75 -2.0 217,161 + EX31_RS11415 0.76 +0.3 217,161 + EX31_RS11415 0.76 +1.3 217,166 + EX31_RS11415 0.77 -1.9 217,289 + +0.3 217,289 + -0.7 217,290 - +2.2 217,290 - +0.8 217,290 - +1.9 217,290 - -1.4 217,290 - -0.2 217,290 - +0.6 217,290 - -1.4 217,290 - -0.5 217,355 + -1.2 217,355 + -0.1 217,355 + -0.8 217,356 - -1.2 217,360 + -0.1 217,361 - +0.5 217,361 - +1.3 217,361 - -2.2 217,364 - -1.2 217,386 - -0.6 217,386 - +1.1 217,460 - +0.2 217,460 - -2.2
Or see this region's nucleotide sequence