Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS11275 and EX31_RS11280 overlap by 1 nucleotides EX31_RS11280 and EX31_RS11285 are separated by 10 nucleotides EX31_RS11285 and EX31_RS11290 are separated by 127 nucleotides
EX31_RS11275: EX31_RS11275 - flagellar assembly peptidoglycan hydrolase FlgJ, at 188,192 to 189,142
_RS11275
EX31_RS11280: EX31_RS11280 - flagellar basal body P-ring protein FlgI, at 189,142 to 190,248
_RS11280
EX31_RS11285: EX31_RS11285 - flagellar basal body L-ring protein FlgH, at 190,259 to 190,963
_RS11285
EX31_RS11290: EX31_RS11290 - flagellar basal-body rod protein FlgG, at 191,091 to 191,873
_RS11290
Position (kb) 189
190
191 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 188.200 kb on + strand at 188.200 kb on + strand at 188.220 kb on + strand at 188.286 kb on + strand at 188.287 kb on - strand, within EX31_RS11275 at 188.344 kb on + strand, within EX31_RS11275 at 188.344 kb on + strand, within EX31_RS11275 at 188.384 kb on - strand, within EX31_RS11275 at 188.397 kb on + strand, within EX31_RS11275 at 188.398 kb on - strand, within EX31_RS11275 at 188.398 kb on - strand, within EX31_RS11275 at 188.398 kb on - strand, within EX31_RS11275 at 188.418 kb on + strand, within EX31_RS11275 at 188.419 kb on - strand, within EX31_RS11275 at 188.419 kb on - strand, within EX31_RS11275 at 188.419 kb on - strand, within EX31_RS11275 at 188.427 kb on + strand, within EX31_RS11275 at 188.427 kb on + strand, within EX31_RS11275 at 188.470 kb on + strand, within EX31_RS11275 at 188.471 kb on - strand, within EX31_RS11275 at 188.471 kb on - strand, within EX31_RS11275 at 188.471 kb on - strand, within EX31_RS11275 at 188.642 kb on - strand, within EX31_RS11275 at 188.755 kb on - strand, within EX31_RS11275 at 188.926 kb on - strand, within EX31_RS11275 at 188.926 kb on - strand, within EX31_RS11275 at 188.926 kb on - strand, within EX31_RS11275 at 188.935 kb on + strand, within EX31_RS11275 at 189.092 kb on - strand at 189.109 kb on - strand at 189.214 kb on - strand at 189.274 kb on + strand, within EX31_RS11280 at 189.552 kb on + strand, within EX31_RS11280 at 189.553 kb on - strand, within EX31_RS11280 at 189.669 kb on + strand, within EX31_RS11280 at 189.669 kb on + strand, within EX31_RS11280 at 189.705 kb on + strand, within EX31_RS11280 at 189.745 kb on - strand, within EX31_RS11280 at 189.766 kb on - strand, within EX31_RS11280 at 189.837 kb on - strand, within EX31_RS11280 at 189.903 kb on + strand, within EX31_RS11280 at 190.047 kb on + strand, within EX31_RS11280 at 190.048 kb on - strand, within EX31_RS11280 at 190.173 kb on + strand at 190.260 kb on + strand at 190.260 kb on + strand at 190.261 kb on - strand at 190.262 kb on + strand at 190.262 kb on + strand at 190.262 kb on + strand at 190.263 kb on - strand at 190.373 kb on - strand, within EX31_RS11285 at 190.582 kb on + strand, within EX31_RS11285 at 190.623 kb on + strand, within EX31_RS11285 at 190.624 kb on - strand, within EX31_RS11285 at 190.741 kb on + strand, within EX31_RS11285 at 190.742 kb on - strand, within EX31_RS11285 at 190.742 kb on - strand, within EX31_RS11285 at 190.742 kb on - strand, within EX31_RS11285 at 190.779 kb on + strand, within EX31_RS11285 at 190.780 kb on - strand, within EX31_RS11285 at 190.885 kb on + strand, within EX31_RS11285 at 190.886 kb on - strand, within EX31_RS11285 at 190.887 kb on + strand, within EX31_RS11285 at 190.887 kb on + strand, within EX31_RS11285 at 190.888 kb on - strand, within EX31_RS11285 at 190.888 kb on - strand, within EX31_RS11285 at 190.888 kb on - strand, within EX31_RS11285 at 190.888 kb on - strand, within EX31_RS11285 at 190.888 kb on - strand, within EX31_RS11285 at 190.888 kb on - strand, within EX31_RS11285 at 190.907 kb on + strand at 190.952 kb on - strand at 191.141 kb on + strand at 191.141 kb on + strand at 191.142 kb on - strand at 191.142 kb on - strand at 191.208 kb on - strand, within EX31_RS11290 at 191.221 kb on + strand, within EX31_RS11290 at 191.222 kb on - strand, within EX31_RS11290 at 191.222 kb on - strand, within EX31_RS11290 at 191.231 kb on - strand, within EX31_RS11290
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 188,200 + +0.7 188,200 + -0.9 188,220 + -0.4 188,286 + -2.0 188,287 - EX31_RS11275 0.10 -2.2 188,344 + EX31_RS11275 0.16 +1.5 188,344 + EX31_RS11275 0.16 -0.9 188,384 - EX31_RS11275 0.20 +0.9 188,397 + EX31_RS11275 0.22 -1.4 188,398 - EX31_RS11275 0.22 +0.4 188,398 - EX31_RS11275 0.22 -1.0 188,398 - EX31_RS11275 0.22 -1.4 188,418 + EX31_RS11275 0.24 -0.4 188,419 - EX31_RS11275 0.24 -2.5 188,419 - EX31_RS11275 0.24 -0.7 188,419 - EX31_RS11275 0.24 +0.1 188,427 + EX31_RS11275 0.25 -1.8 188,427 + EX31_RS11275 0.25 +0.9 188,470 + EX31_RS11275 0.29 +0.9 188,471 - EX31_RS11275 0.29 -0.8 188,471 - EX31_RS11275 0.29 -1.1 188,471 - EX31_RS11275 0.29 +0.6 188,642 - EX31_RS11275 0.47 -1.1 188,755 - EX31_RS11275 0.59 -1.8 188,926 - EX31_RS11275 0.77 -0.3 188,926 - EX31_RS11275 0.77 -1.1 188,926 - EX31_RS11275 0.77 -2.3 188,935 + EX31_RS11275 0.78 +2.1 189,092 - -1.1 189,109 - -0.2 189,214 - -1.3 189,274 + EX31_RS11280 0.12 -1.0 189,552 + EX31_RS11280 0.37 +0.9 189,553 - EX31_RS11280 0.37 -0.7 189,669 + EX31_RS11280 0.48 +0.5 189,669 + EX31_RS11280 0.48 -1.4 189,705 + EX31_RS11280 0.51 +0.1 189,745 - EX31_RS11280 0.54 -0.9 189,766 - EX31_RS11280 0.56 -0.2 189,837 - EX31_RS11280 0.63 +0.3 189,903 + EX31_RS11280 0.69 +0.3 190,047 + EX31_RS11280 0.82 -1.2 190,048 - EX31_RS11280 0.82 -0.9 190,173 + +0.3 190,260 + -0.5 190,260 + -0.1 190,261 - -1.5 190,262 + -1.4 190,262 + -0.7 190,262 + -0.5 190,263 - -0.1 190,373 - EX31_RS11285 0.16 +0.1 190,582 + EX31_RS11285 0.46 -0.6 190,623 + EX31_RS11285 0.52 +1.9 190,624 - EX31_RS11285 0.52 -0.4 190,741 + EX31_RS11285 0.68 -0.7 190,742 - EX31_RS11285 0.69 -0.9 190,742 - EX31_RS11285 0.69 +3.5 190,742 - EX31_RS11285 0.69 -2.0 190,779 + EX31_RS11285 0.74 -0.7 190,780 - EX31_RS11285 0.74 -0.9 190,885 + EX31_RS11285 0.89 -2.5 190,886 - EX31_RS11285 0.89 -1.1 190,887 + EX31_RS11285 0.89 +0.5 190,887 + EX31_RS11285 0.89 -0.1 190,888 - EX31_RS11285 0.89 +0.6 190,888 - EX31_RS11285 0.89 +0.3 190,888 - EX31_RS11285 0.89 -0.7 190,888 - EX31_RS11285 0.89 -0.1 190,888 - EX31_RS11285 0.89 -0.4 190,888 - EX31_RS11285 0.89 -1.8 190,907 + -1.7 190,952 - -0.1 191,141 + -0.7 191,141 + -0.8 191,142 - -0.7 191,142 - -1.8 191,208 - EX31_RS11290 0.15 -1.5 191,221 + EX31_RS11290 0.17 -0.9 191,222 - EX31_RS11290 0.17 +0.3 191,222 - EX31_RS11290 0.17 -0.3 191,231 - EX31_RS11290 0.18 -0.2
Or see this region's nucleotide sequence