Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS10705 and EX31_RS10710 are separated by 49 nucleotides EX31_RS10710 and EX31_RS10715 overlap by 4 nucleotides EX31_RS10715 and EX31_RS10720 are separated by 216 nucleotides EX31_RS10720 and EX31_RS10725 are separated by 55 nucleotides EX31_RS10725 and EX31_RS10730 are separated by 98 nucleotides
EX31_RS10705: EX31_RS10705 - adhesin, at 58,080 to 59,216
_RS10705
EX31_RS10710: EX31_RS10710 - hypothetical protein, at 59,266 to 59,682
_RS10710
EX31_RS10715: EX31_RS10715 - hypothetical protein, at 59,679 to 59,903
_RS10715
EX31_RS10720: EX31_RS10720 - NAD(P)H:quinone oxidoreductase, at 60,120 to 60,809
_RS10720
EX31_RS10725: EX31_RS10725 - plasmid maintenance protein CcdB, at 60,865 to 61,053
_RS10725
EX31_RS10730: EX31_RS10730 - elongation factor G, at 61,152 to 63,257
_RS10730
Position (kb)
60
61 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 59.177 kb on + strand at 59.178 kb on - strand at 59.178 kb on - strand at 59.214 kb on + strand at 59.215 kb on - strand at 59.317 kb on - strand, within EX31_RS10710 at 59.374 kb on + strand, within EX31_RS10710 at 59.374 kb on + strand, within EX31_RS10710 at 59.374 kb on + strand, within EX31_RS10710 at 59.374 kb on + strand, within EX31_RS10710 at 59.374 kb on + strand, within EX31_RS10710 at 59.374 kb on + strand, within EX31_RS10710 at 59.375 kb on - strand, within EX31_RS10710 at 59.375 kb on - strand, within EX31_RS10710 at 59.375 kb on - strand, within EX31_RS10710 at 59.375 kb on - strand, within EX31_RS10710 at 59.375 kb on - strand, within EX31_RS10710 at 59.375 kb on - strand, within EX31_RS10710 at 59.375 kb on - strand, within EX31_RS10710 at 59.377 kb on - strand, within EX31_RS10710 at 59.377 kb on - strand, within EX31_RS10710 at 59.377 kb on - strand, within EX31_RS10710 at 59.377 kb on - strand, within EX31_RS10710 at 59.452 kb on + strand, within EX31_RS10710 at 59.490 kb on + strand, within EX31_RS10710 at 59.496 kb on + strand, within EX31_RS10710 at 59.533 kb on + strand, within EX31_RS10710 at 59.533 kb on + strand, within EX31_RS10710 at 59.533 kb on + strand, within EX31_RS10710 at 59.534 kb on - strand, within EX31_RS10710 at 59.535 kb on + strand, within EX31_RS10710 at 59.536 kb on - strand, within EX31_RS10710 at 59.615 kb on - strand, within EX31_RS10710 at 59.615 kb on - strand, within EX31_RS10710 at 59.617 kb on + strand, within EX31_RS10710 at 59.617 kb on + strand, within EX31_RS10710 at 59.662 kb on + strand at 59.730 kb on + strand, within EX31_RS10715 at 59.730 kb on + strand, within EX31_RS10715 at 59.730 kb on + strand, within EX31_RS10715 at 59.731 kb on - strand, within EX31_RS10715 at 59.731 kb on - strand, within EX31_RS10715 at 59.731 kb on - strand, within EX31_RS10715 at 59.731 kb on - strand, within EX31_RS10715 at 59.731 kb on - strand, within EX31_RS10715 at 59.732 kb on + strand, within EX31_RS10715 at 59.732 kb on + strand, within EX31_RS10715 at 59.732 kb on + strand, within EX31_RS10715 at 59.732 kb on + strand, within EX31_RS10715 at 59.732 kb on + strand, within EX31_RS10715 at 59.732 kb on + strand, within EX31_RS10715 at 59.733 kb on - strand, within EX31_RS10715 at 59.733 kb on - strand, within EX31_RS10715 at 59.923 kb on + strand at 59.923 kb on + strand at 59.924 kb on - strand at 59.925 kb on + strand at 59.967 kb on + strand at 59.968 kb on - strand at 59.968 kb on - strand at 59.970 kb on + strand at 59.970 kb on + strand at 59.970 kb on + strand at 59.971 kb on - strand at 59.971 kb on - strand at 59.971 kb on - strand at 59.971 kb on - strand at 59.971 kb on - strand at 59.972 kb on + strand at 59.972 kb on + strand at 59.973 kb on - strand at 59.973 kb on - strand at 59.973 kb on - strand at 59.973 kb on - strand at 59.974 kb on + strand at 59.974 kb on + strand at 59.974 kb on + strand at 59.974 kb on + strand at 59.975 kb on - strand at 59.975 kb on - strand at 59.975 kb on - strand at 59.975 kb on - strand at 59.975 kb on - strand at 60.015 kb on + strand at 60.016 kb on - strand at 60.016 kb on - strand at 60.016 kb on - strand at 60.048 kb on + strand at 60.049 kb on - strand at 60.149 kb on + strand at 60.205 kb on + strand, within EX31_RS10720 at 60.243 kb on + strand, within EX31_RS10720 at 60.243 kb on + strand, within EX31_RS10720 at 60.243 kb on + strand, within EX31_RS10720 at 60.243 kb on + strand, within EX31_RS10720 at 60.243 kb on + strand, within EX31_RS10720 at 60.244 kb on - strand, within EX31_RS10720 at 60.244 kb on - strand, within EX31_RS10720 at 60.244 kb on - strand, within EX31_RS10720 at 60.244 kb on - strand, within EX31_RS10720 at 60.244 kb on - strand, within EX31_RS10720 at 60.316 kb on + strand, within EX31_RS10720 at 60.418 kb on - strand, within EX31_RS10720 at 60.542 kb on - strand, within EX31_RS10720 at 60.542 kb on - strand, within EX31_RS10720 at 60.542 kb on - strand, within EX31_RS10720 at 60.584 kb on + strand, within EX31_RS10720 at 60.584 kb on + strand, within EX31_RS10720 at 60.584 kb on + strand, within EX31_RS10720 at 60.760 kb on + strand at 60.918 kb on + strand, within EX31_RS10725 at 60.930 kb on + strand, within EX31_RS10725 at 60.989 kb on - strand, within EX31_RS10725 at 61.040 kb on - strand at 61.042 kb on + strand at 61.042 kb on + strand at 61.067 kb on - strand at 61.067 kb on - strand at 61.069 kb on + strand at 61.070 kb on - strand at 61.070 kb on - strand at 61.138 kb on + strand at 61.157 kb on + strand at 61.158 kb on - strand at 61.158 kb on - strand at 61.158 kb on - strand at 61.229 kb on - strand at 61.229 kb on - strand at 61.229 kb on - strand at 61.271 kb on - strand at 61.353 kb on - strand at 61.526 kb on - strand, within EX31_RS10730 at 61.628 kb on - strand, within EX31_RS10730 at 61.628 kb on - strand, within EX31_RS10730 at 61.628 kb on - strand, within EX31_RS10730 at 61.628 kb on - strand, within EX31_RS10730 at 61.628 kb on - strand, within EX31_RS10730
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 59,177 + +1.1 59,178 - +0.3 59,178 - -1.5 59,214 + +1.0 59,215 - +0.9 59,317 - EX31_RS10710 0.12 -1.0 59,374 + EX31_RS10710 0.26 +0.5 59,374 + EX31_RS10710 0.26 +0.7 59,374 + EX31_RS10710 0.26 +0.7 59,374 + EX31_RS10710 0.26 -0.1 59,374 + EX31_RS10710 0.26 -1.1 59,374 + EX31_RS10710 0.26 +1.7 59,375 - EX31_RS10710 0.26 +0.7 59,375 - EX31_RS10710 0.26 +1.2 59,375 - EX31_RS10710 0.26 +0.4 59,375 - EX31_RS10710 0.26 -0.7 59,375 - EX31_RS10710 0.26 -0.1 59,375 - EX31_RS10710 0.26 +1.0 59,375 - EX31_RS10710 0.26 +2.3 59,377 - EX31_RS10710 0.27 +0.7 59,377 - EX31_RS10710 0.27 +2.5 59,377 - EX31_RS10710 0.27 +0.0 59,377 - EX31_RS10710 0.27 +1.2 59,452 + EX31_RS10710 0.45 +0.5 59,490 + EX31_RS10710 0.54 +0.4 59,496 + EX31_RS10710 0.55 +0.3 59,533 + EX31_RS10710 0.64 +0.3 59,533 + EX31_RS10710 0.64 +0.2 59,533 + EX31_RS10710 0.64 +0.7 59,534 - EX31_RS10710 0.64 -1.4 59,535 + EX31_RS10710 0.65 +1.2 59,536 - EX31_RS10710 0.65 +1.9 59,615 - EX31_RS10710 0.84 +0.0 59,615 - EX31_RS10710 0.84 +0.6 59,617 + EX31_RS10710 0.84 +0.1 59,617 + EX31_RS10710 0.84 +1.0 59,662 + +0.7 59,730 + EX31_RS10715 0.23 -0.4 59,730 + EX31_RS10715 0.23 +3.2 59,730 + EX31_RS10715 0.23 +0.6 59,731 - EX31_RS10715 0.23 +1.7 59,731 - EX31_RS10715 0.23 -1.1 59,731 - EX31_RS10715 0.23 +0.3 59,731 - EX31_RS10715 0.23 +1.5 59,731 - EX31_RS10715 0.23 +1.5 59,732 + EX31_RS10715 0.24 -0.1 59,732 + EX31_RS10715 0.24 +0.7 59,732 + EX31_RS10715 0.24 +0.2 59,732 + EX31_RS10715 0.24 -0.4 59,732 + EX31_RS10715 0.24 -0.4 59,732 + EX31_RS10715 0.24 +0.9 59,733 - EX31_RS10715 0.24 +0.1 59,733 - EX31_RS10715 0.24 +0.9 59,923 + +0.0 59,923 + +0.8 59,924 - +0.5 59,925 + +0.1 59,967 + +0.9 59,968 - +1.2 59,968 - +0.6 59,970 + +0.9 59,970 + +0.7 59,970 + +0.3 59,971 - -0.5 59,971 - +1.1 59,971 - +0.4 59,971 - -0.2 59,971 - +0.5 59,972 + +0.1 59,972 + +0.5 59,973 - -1.4 59,973 - +0.8 59,973 - -1.9 59,973 - +0.7 59,974 + +1.2 59,974 + +1.2 59,974 + +0.2 59,974 + -0.1 59,975 - +0.7 59,975 - -1.1 59,975 - +2.1 59,975 - -0.4 59,975 - +1.5 60,015 + -2.2 60,016 - +1.2 60,016 - +0.2 60,016 - +0.8 60,048 + -0.3 60,049 - +1.4 60,149 + +1.4 60,205 + EX31_RS10720 0.12 +0.3 60,243 + EX31_RS10720 0.18 +0.3 60,243 + EX31_RS10720 0.18 -0.9 60,243 + EX31_RS10720 0.18 +0.7 60,243 + EX31_RS10720 0.18 +1.2 60,243 + EX31_RS10720 0.18 -0.4 60,244 - EX31_RS10720 0.18 -1.7 60,244 - EX31_RS10720 0.18 +2.8 60,244 - EX31_RS10720 0.18 -0.2 60,244 - EX31_RS10720 0.18 -0.2 60,244 - EX31_RS10720 0.18 +1.3 60,316 + EX31_RS10720 0.28 +1.9 60,418 - EX31_RS10720 0.43 +0.5 60,542 - EX31_RS10720 0.61 +0.9 60,542 - EX31_RS10720 0.61 +1.9 60,542 - EX31_RS10720 0.61 -0.3 60,584 + EX31_RS10720 0.67 +1.9 60,584 + EX31_RS10720 0.67 -0.1 60,584 + EX31_RS10720 0.67 +0.4 60,760 + +1.5 60,918 + EX31_RS10725 0.28 -0.2 60,930 + EX31_RS10725 0.34 +1.1 60,989 - EX31_RS10725 0.66 +0.0 61,040 - +0.0 61,042 + -0.9 61,042 + +2.0 61,067 - +0.9 61,067 - -1.1 61,069 + +0.9 61,070 - +1.3 61,070 - -0.5 61,138 + +0.7 61,157 + +0.2 61,158 - -1.6 61,158 - +1.2 61,158 - +0.7 61,229 - -1.1 61,229 - +0.5 61,229 - +0.4 61,271 - +0.9 61,353 - -1.7 61,526 - EX31_RS10730 0.18 -0.3 61,628 - EX31_RS10730 0.23 +0.6 61,628 - EX31_RS10730 0.23 -0.2 61,628 - EX31_RS10730 0.23 -0.1 61,628 - EX31_RS10730 0.23 -0.7 61,628 - EX31_RS10730 0.23 +0.1
Or see this region's nucleotide sequence