Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS05500 and EX31_RS05505 are separated by 10 nucleotides EX31_RS05505 and EX31_RS05510 are separated by 10 nucleotides
EX31_RS05500: EX31_RS05500 - dipeptide ABC transporter ATP-binding protein, at 252,131 to 253,156
_RS05500
EX31_RS05505: EX31_RS05505 - dipeptide ABC transporter permease DppC, at 253,167 to 254,081
_RS05505
EX31_RS05510: EX31_RS05510 - dipeptide ABC transporter permease DppB, at 254,092 to 255,111
_RS05510
Position (kb)
253
254
255 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 252.226 kb on + strand at 252.246 kb on + strand, within EX31_RS05500 at 252.246 kb on + strand, within EX31_RS05500 at 252.247 kb on - strand, within EX31_RS05500 at 252.277 kb on + strand, within EX31_RS05500 at 252.288 kb on + strand, within EX31_RS05500 at 252.288 kb on + strand, within EX31_RS05500 at 252.288 kb on + strand, within EX31_RS05500 at 252.288 kb on + strand, within EX31_RS05500 at 252.288 kb on + strand, within EX31_RS05500 at 252.288 kb on + strand, within EX31_RS05500 at 252.288 kb on + strand, within EX31_RS05500 at 252.288 kb on + strand, within EX31_RS05500 at 252.288 kb on + strand, within EX31_RS05500 at 252.288 kb on + strand, within EX31_RS05500 at 252.288 kb on + strand, within EX31_RS05500 at 252.288 kb on + strand, within EX31_RS05500 at 252.289 kb on - strand, within EX31_RS05500 at 252.289 kb on - strand, within EX31_RS05500 at 252.289 kb on - strand, within EX31_RS05500 at 252.289 kb on - strand, within EX31_RS05500 at 252.331 kb on + strand, within EX31_RS05500 at 252.332 kb on - strand, within EX31_RS05500 at 252.376 kb on - strand, within EX31_RS05500 at 252.376 kb on - strand, within EX31_RS05500 at 252.376 kb on - strand, within EX31_RS05500 at 252.425 kb on + strand, within EX31_RS05500 at 252.426 kb on - strand, within EX31_RS05500 at 252.426 kb on - strand, within EX31_RS05500 at 252.426 kb on - strand, within EX31_RS05500 at 252.426 kb on - strand, within EX31_RS05500 at 252.438 kb on + strand, within EX31_RS05500 at 252.438 kb on + strand, within EX31_RS05500 at 252.438 kb on + strand, within EX31_RS05500 at 252.438 kb on + strand, within EX31_RS05500 at 252.438 kb on + strand, within EX31_RS05500 at 252.438 kb on + strand, within EX31_RS05500 at 252.439 kb on - strand, within EX31_RS05500 at 252.439 kb on - strand, within EX31_RS05500 at 252.439 kb on - strand, within EX31_RS05500 at 252.439 kb on - strand, within EX31_RS05500 at 252.439 kb on - strand, within EX31_RS05500 at 252.439 kb on - strand, within EX31_RS05500 at 252.448 kb on + strand, within EX31_RS05500 at 252.448 kb on + strand, within EX31_RS05500 at 252.449 kb on - strand, within EX31_RS05500 at 252.449 kb on - strand, within EX31_RS05500 at 252.599 kb on + strand, within EX31_RS05500 at 252.599 kb on + strand, within EX31_RS05500 at 252.600 kb on - strand, within EX31_RS05500 at 252.672 kb on + strand, within EX31_RS05500 at 252.672 kb on + strand, within EX31_RS05500 at 252.673 kb on - strand, within EX31_RS05500 at 252.784 kb on + strand, within EX31_RS05500 at 252.804 kb on + strand, within EX31_RS05500 at 252.804 kb on + strand, within EX31_RS05500 at 252.804 kb on + strand, within EX31_RS05500 at 252.805 kb on - strand, within EX31_RS05500 at 252.901 kb on + strand, within EX31_RS05500 at 253.038 kb on + strand, within EX31_RS05500 at 253.038 kb on + strand, within EX31_RS05500 at 253.039 kb on - strand, within EX31_RS05500 at 253.039 kb on - strand, within EX31_RS05500 at 253.046 kb on - strand, within EX31_RS05500 at 253.052 kb on + strand, within EX31_RS05500 at 253.052 kb on + strand, within EX31_RS05500 at 253.099 kb on + strand at 253.175 kb on + strand at 253.175 kb on + strand at 253.175 kb on + strand at 253.175 kb on + strand at 253.175 kb on + strand at 253.175 kb on + strand at 253.176 kb on - strand at 253.176 kb on - strand at 253.176 kb on - strand at 253.176 kb on - strand at 253.176 kb on - strand at 253.176 kb on - strand at 253.176 kb on - strand at 253.176 kb on - strand at 253.220 kb on + strand at 253.221 kb on - strand at 253.221 kb on - strand at 253.293 kb on + strand, within EX31_RS05505 at 253.293 kb on + strand, within EX31_RS05505 at 253.294 kb on - strand, within EX31_RS05505 at 253.430 kb on + strand, within EX31_RS05505 at 253.431 kb on - strand, within EX31_RS05505 at 253.431 kb on - strand, within EX31_RS05505 at 253.431 kb on - strand, within EX31_RS05505 at 253.431 kb on - strand, within EX31_RS05505 at 253.431 kb on - strand, within EX31_RS05505 at 253.431 kb on - strand, within EX31_RS05505 at 253.435 kb on - strand, within EX31_RS05505 at 253.435 kb on - strand, within EX31_RS05505 at 253.439 kb on + strand, within EX31_RS05505 at 253.440 kb on - strand, within EX31_RS05505 at 253.440 kb on - strand, within EX31_RS05505 at 253.440 kb on - strand, within EX31_RS05505 at 253.440 kb on - strand, within EX31_RS05505 at 253.495 kb on + strand, within EX31_RS05505 at 253.496 kb on - strand, within EX31_RS05505 at 253.496 kb on - strand, within EX31_RS05505 at 253.543 kb on + strand, within EX31_RS05505 at 253.543 kb on + strand, within EX31_RS05505 at 253.543 kb on + strand, within EX31_RS05505 at 253.543 kb on + strand, within EX31_RS05505 at 253.543 kb on + strand, within EX31_RS05505 at 253.544 kb on - strand, within EX31_RS05505 at 253.544 kb on - strand, within EX31_RS05505 at 253.544 kb on - strand, within EX31_RS05505 at 253.544 kb on - strand, within EX31_RS05505 at 253.544 kb on - strand, within EX31_RS05505 at 253.544 kb on - strand, within EX31_RS05505 at 253.544 kb on - strand, within EX31_RS05505 at 253.544 kb on - strand, within EX31_RS05505 at 253.544 kb on - strand, within EX31_RS05505 at 253.544 kb on - strand, within EX31_RS05505 at 253.544 kb on - strand, within EX31_RS05505 at 253.652 kb on + strand, within EX31_RS05505 at 253.693 kb on + strand, within EX31_RS05505 at 253.694 kb on - strand, within EX31_RS05505 at 253.694 kb on - strand, within EX31_RS05505 at 253.740 kb on + strand, within EX31_RS05505 at 253.741 kb on - strand, within EX31_RS05505 at 253.783 kb on + strand, within EX31_RS05505 at 253.783 kb on + strand, within EX31_RS05505 at 253.783 kb on + strand, within EX31_RS05505 at 253.784 kb on - strand, within EX31_RS05505 at 253.803 kb on - strand, within EX31_RS05505 at 253.949 kb on + strand, within EX31_RS05505 at 253.949 kb on + strand, within EX31_RS05505 at 253.949 kb on + strand, within EX31_RS05505 at 253.949 kb on + strand, within EX31_RS05505 at 253.949 kb on + strand, within EX31_RS05505 at 253.950 kb on - strand, within EX31_RS05505 at 253.950 kb on - strand, within EX31_RS05505 at 253.950 kb on - strand, within EX31_RS05505 at 253.950 kb on - strand, within EX31_RS05505 at 253.957 kb on + strand, within EX31_RS05505 at 253.957 kb on + strand, within EX31_RS05505 at 253.957 kb on + strand, within EX31_RS05505 at 253.957 kb on + strand, within EX31_RS05505 at 253.958 kb on - strand, within EX31_RS05505 at 253.958 kb on - strand, within EX31_RS05505 at 253.958 kb on - strand, within EX31_RS05505 at 253.958 kb on - strand, within EX31_RS05505 at 253.958 kb on - strand, within EX31_RS05505 at 253.996 kb on + strand at 253.997 kb on - strand at 253.997 kb on - strand at 254.047 kb on - strand at 254.047 kb on - strand at 254.093 kb on + strand at 254.094 kb on - strand at 254.110 kb on - strand at 254.130 kb on - strand at 254.140 kb on + strand at 254.140 kb on + strand at 254.140 kb on + strand at 254.140 kb on + strand at 254.140 kb on + strand at 254.140 kb on + strand at 254.140 kb on + strand at 254.141 kb on - strand at 254.141 kb on - strand at 254.141 kb on - strand at 254.169 kb on + strand at 254.170 kb on - strand at 254.205 kb on - strand, within EX31_RS05510 at 254.205 kb on - strand, within EX31_RS05510 at 254.296 kb on - strand, within EX31_RS05510 at 254.296 kb on - strand, within EX31_RS05510 at 254.370 kb on + strand, within EX31_RS05510 at 254.405 kb on + strand, within EX31_RS05510 at 254.405 kb on + strand, within EX31_RS05510 at 254.405 kb on + strand, within EX31_RS05510 at 254.405 kb on + strand, within EX31_RS05510 at 254.405 kb on + strand, within EX31_RS05510 at 254.405 kb on + strand, within EX31_RS05510 at 254.405 kb on + strand, within EX31_RS05510 at 254.406 kb on - strand, within EX31_RS05510 at 254.406 kb on - strand, within EX31_RS05510 at 254.406 kb on - strand, within EX31_RS05510 at 254.406 kb on - strand, within EX31_RS05510 at 254.406 kb on - strand, within EX31_RS05510 at 254.406 kb on - strand, within EX31_RS05510 at 254.406 kb on - strand, within EX31_RS05510 at 254.406 kb on - strand, within EX31_RS05510 at 254.406 kb on - strand, within EX31_RS05510 at 254.406 kb on - strand, within EX31_RS05510 at 254.445 kb on + strand, within EX31_RS05510 at 254.445 kb on + strand, within EX31_RS05510 at 254.446 kb on - strand, within EX31_RS05510 at 254.446 kb on - strand, within EX31_RS05510 at 254.480 kb on - strand, within EX31_RS05510 at 254.559 kb on + strand, within EX31_RS05510 at 254.559 kb on + strand, within EX31_RS05510 at 254.560 kb on - strand, within EX31_RS05510 at 254.560 kb on - strand, within EX31_RS05510 at 254.632 kb on - strand, within EX31_RS05510 at 254.632 kb on - strand, within EX31_RS05510 at 254.632 kb on - strand, within EX31_RS05510 at 254.677 kb on + strand, within EX31_RS05510 at 254.690 kb on + strand, within EX31_RS05510 at 254.833 kb on + strand, within EX31_RS05510 at 254.907 kb on - strand, within EX31_RS05510 at 254.907 kb on - strand, within EX31_RS05510 at 254.915 kb on + strand, within EX31_RS05510 at 254.915 kb on + strand, within EX31_RS05510 at 254.915 kb on + strand, within EX31_RS05510 at 254.916 kb on - strand, within EX31_RS05510 at 254.916 kb on - strand, within EX31_RS05510 at 254.916 kb on - strand, within EX31_RS05510 at 254.924 kb on + strand, within EX31_RS05510 at 254.946 kb on + strand, within EX31_RS05510 at 254.946 kb on + strand, within EX31_RS05510 at 255.022 kb on + strand at 255.023 kb on - strand at 255.023 kb on - strand at 255.031 kb on + strand at 255.031 kb on + strand at 255.031 kb on + strand at 255.031 kb on + strand at 255.031 kb on + strand at 255.031 kb on + strand at 255.032 kb on - strand at 255.032 kb on - strand at 255.032 kb on - strand at 255.032 kb on - strand at 255.032 kb on - strand at 255.032 kb on - strand at 255.032 kb on - strand at 255.054 kb on + strand at 255.054 kb on + strand at 255.054 kb on + strand at 255.054 kb on + strand at 255.054 kb on + strand at 255.054 kb on + strand at 255.054 kb on + strand at 255.054 kb on + strand at 255.055 kb on - strand at 255.055 kb on - strand at 255.076 kb on - strand at 255.076 kb on - strand at 255.079 kb on + strand at 255.079 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 252,226 + +1.5 252,246 + EX31_RS05500 0.11 -0.7 252,246 + EX31_RS05500 0.11 -0.2 252,247 - EX31_RS05500 0.11 -1.6 252,277 + EX31_RS05500 0.14 +0.4 252,288 + EX31_RS05500 0.15 -0.6 252,288 + EX31_RS05500 0.15 -0.3 252,288 + EX31_RS05500 0.15 -0.9 252,288 + EX31_RS05500 0.15 +0.1 252,288 + EX31_RS05500 0.15 -0.1 252,288 + EX31_RS05500 0.15 -1.3 252,288 + EX31_RS05500 0.15 +0.5 252,288 + EX31_RS05500 0.15 -0.0 252,288 + EX31_RS05500 0.15 +0.1 252,288 + EX31_RS05500 0.15 -1.2 252,288 + EX31_RS05500 0.15 -0.1 252,288 + EX31_RS05500 0.15 -0.0 252,289 - EX31_RS05500 0.15 +0.2 252,289 - EX31_RS05500 0.15 -0.7 252,289 - EX31_RS05500 0.15 -0.7 252,289 - EX31_RS05500 0.15 +0.9 252,331 + EX31_RS05500 0.19 +1.0 252,332 - EX31_RS05500 0.20 +0.8 252,376 - EX31_RS05500 0.24 +0.4 252,376 - EX31_RS05500 0.24 -0.4 252,376 - EX31_RS05500 0.24 -0.2 252,425 + EX31_RS05500 0.29 +0.7 252,426 - EX31_RS05500 0.29 -0.2 252,426 - EX31_RS05500 0.29 +0.4 252,426 - EX31_RS05500 0.29 -0.3 252,426 - EX31_RS05500 0.29 +0.1 252,438 + EX31_RS05500 0.30 +0.5 252,438 + EX31_RS05500 0.30 -0.4 252,438 + EX31_RS05500 0.30 +0.0 252,438 + EX31_RS05500 0.30 +0.2 252,438 + EX31_RS05500 0.30 -0.4 252,438 + EX31_RS05500 0.30 +0.1 252,439 - EX31_RS05500 0.30 -0.1 252,439 - EX31_RS05500 0.30 +0.9 252,439 - EX31_RS05500 0.30 +0.0 252,439 - EX31_RS05500 0.30 +2.3 252,439 - EX31_RS05500 0.30 +0.8 252,439 - EX31_RS05500 0.30 -0.1 252,448 + EX31_RS05500 0.31 -1.4 252,448 + EX31_RS05500 0.31 +0.9 252,449 - EX31_RS05500 0.31 -0.5 252,449 - EX31_RS05500 0.31 +0.4 252,599 + EX31_RS05500 0.46 +0.9 252,599 + EX31_RS05500 0.46 +0.1 252,600 - EX31_RS05500 0.46 -0.1 252,672 + EX31_RS05500 0.53 +0.3 252,672 + EX31_RS05500 0.53 -1.1 252,673 - EX31_RS05500 0.53 -0.5 252,784 + EX31_RS05500 0.64 -1.1 252,804 + EX31_RS05500 0.66 +0.1 252,804 + EX31_RS05500 0.66 -0.2 252,804 + EX31_RS05500 0.66 +0.1 252,805 - EX31_RS05500 0.66 +0.5 252,901 + EX31_RS05500 0.75 +0.2 253,038 + EX31_RS05500 0.88 -1.0 253,038 + EX31_RS05500 0.88 -0.9 253,039 - EX31_RS05500 0.88 -0.0 253,039 - EX31_RS05500 0.88 -0.1 253,046 - EX31_RS05500 0.89 -0.5 253,052 + EX31_RS05500 0.90 -1.2 253,052 + EX31_RS05500 0.90 +0.4 253,099 + +0.5 253,175 + +0.2 253,175 + +0.5 253,175 + -0.0 253,175 + -0.6 253,175 + +0.8 253,175 + -2.0 253,176 - +0.8 253,176 - -0.0 253,176 - +0.2 253,176 - -0.2 253,176 - -0.7 253,176 - +0.4 253,176 - +0.4 253,176 - -0.7 253,220 + +1.0 253,221 - +0.5 253,221 - -0.7 253,293 + EX31_RS05505 0.14 -0.0 253,293 + EX31_RS05505 0.14 +0.6 253,294 - EX31_RS05505 0.14 -0.2 253,430 + EX31_RS05505 0.29 -0.3 253,431 - EX31_RS05505 0.29 -1.0 253,431 - EX31_RS05505 0.29 -0.5 253,431 - EX31_RS05505 0.29 -0.9 253,431 - EX31_RS05505 0.29 -0.0 253,431 - EX31_RS05505 0.29 -0.0 253,431 - EX31_RS05505 0.29 +0.8 253,435 - EX31_RS05505 0.29 -0.1 253,435 - EX31_RS05505 0.29 -0.2 253,439 + EX31_RS05505 0.30 -1.3 253,440 - EX31_RS05505 0.30 +0.4 253,440 - EX31_RS05505 0.30 -0.6 253,440 - EX31_RS05505 0.30 -0.5 253,440 - EX31_RS05505 0.30 -2.0 253,495 + EX31_RS05505 0.36 +0.3 253,496 - EX31_RS05505 0.36 +1.1 253,496 - EX31_RS05505 0.36 -0.1 253,543 + EX31_RS05505 0.41 +0.1 253,543 + EX31_RS05505 0.41 -0.2 253,543 + EX31_RS05505 0.41 -0.4 253,543 + EX31_RS05505 0.41 +1.3 253,543 + EX31_RS05505 0.41 +0.8 253,544 - EX31_RS05505 0.41 +1.1 253,544 - EX31_RS05505 0.41 -0.7 253,544 - EX31_RS05505 0.41 -0.1 253,544 - EX31_RS05505 0.41 -0.3 253,544 - EX31_RS05505 0.41 +0.6 253,544 - EX31_RS05505 0.41 -0.1 253,544 - EX31_RS05505 0.41 -0.4 253,544 - EX31_RS05505 0.41 +0.3 253,544 - EX31_RS05505 0.41 +0.3 253,544 - EX31_RS05505 0.41 -0.4 253,544 - EX31_RS05505 0.41 -0.7 253,652 + EX31_RS05505 0.53 -0.4 253,693 + EX31_RS05505 0.57 -0.1 253,694 - EX31_RS05505 0.58 +0.1 253,694 - EX31_RS05505 0.58 -1.0 253,740 + EX31_RS05505 0.63 +0.6 253,741 - EX31_RS05505 0.63 -0.3 253,783 + EX31_RS05505 0.67 +0.2 253,783 + EX31_RS05505 0.67 +0.2 253,783 + EX31_RS05505 0.67 +0.5 253,784 - EX31_RS05505 0.67 -1.0 253,803 - EX31_RS05505 0.70 -0.8 253,949 + EX31_RS05505 0.85 +0.3 253,949 + EX31_RS05505 0.85 -1.2 253,949 + EX31_RS05505 0.85 -1.3 253,949 + EX31_RS05505 0.85 +0.2 253,949 + EX31_RS05505 0.85 -0.6 253,950 - EX31_RS05505 0.86 -0.0 253,950 - EX31_RS05505 0.86 -0.4 253,950 - EX31_RS05505 0.86 -0.4 253,950 - EX31_RS05505 0.86 +0.1 253,957 + EX31_RS05505 0.86 +1.1 253,957 + EX31_RS05505 0.86 -0.3 253,957 + EX31_RS05505 0.86 +3.3 253,957 + EX31_RS05505 0.86 +0.0 253,958 - EX31_RS05505 0.86 +0.8 253,958 - EX31_RS05505 0.86 -0.4 253,958 - EX31_RS05505 0.86 +0.8 253,958 - EX31_RS05505 0.86 +0.2 253,958 - EX31_RS05505 0.86 +0.2 253,996 + -0.9 253,997 - -0.9 253,997 - +0.1 254,047 - +1.4 254,047 - -0.6 254,093 + +1.8 254,094 - -0.2 254,110 - -1.1 254,130 - -0.4 254,140 + -0.8 254,140 + -0.8 254,140 + -1.3 254,140 + -0.2 254,140 + -0.5 254,140 + -0.7 254,140 + +0.7 254,141 - -0.4 254,141 - +0.6 254,141 - -0.9 254,169 + +0.5 254,170 - +0.4 254,205 - EX31_RS05510 0.11 +0.2 254,205 - EX31_RS05510 0.11 +0.8 254,296 - EX31_RS05510 0.20 +0.1 254,296 - EX31_RS05510 0.20 +0.7 254,370 + EX31_RS05510 0.27 -1.9 254,405 + EX31_RS05510 0.31 +2.1 254,405 + EX31_RS05510 0.31 -0.1 254,405 + EX31_RS05510 0.31 -0.8 254,405 + EX31_RS05510 0.31 +0.2 254,405 + EX31_RS05510 0.31 +0.4 254,405 + EX31_RS05510 0.31 -1.5 254,405 + EX31_RS05510 0.31 -0.1 254,406 - EX31_RS05510 0.31 -0.0 254,406 - EX31_RS05510 0.31 +0.2 254,406 - EX31_RS05510 0.31 -0.3 254,406 - EX31_RS05510 0.31 +0.2 254,406 - EX31_RS05510 0.31 -0.5 254,406 - EX31_RS05510 0.31 -0.1 254,406 - EX31_RS05510 0.31 +2.3 254,406 - EX31_RS05510 0.31 -2.7 254,406 - EX31_RS05510 0.31 -1.1 254,406 - EX31_RS05510 0.31 -0.7 254,445 + EX31_RS05510 0.35 -0.8 254,445 + EX31_RS05510 0.35 -0.1 254,446 - EX31_RS05510 0.35 +0.4 254,446 - EX31_RS05510 0.35 -0.8 254,480 - EX31_RS05510 0.38 -0.1 254,559 + EX31_RS05510 0.46 -0.1 254,559 + EX31_RS05510 0.46 -0.0 254,560 - EX31_RS05510 0.46 -0.2 254,560 - EX31_RS05510 0.46 -0.6 254,632 - EX31_RS05510 0.53 +1.0 254,632 - EX31_RS05510 0.53 -0.8 254,632 - EX31_RS05510 0.53 -0.4 254,677 + EX31_RS05510 0.57 -0.9 254,690 + EX31_RS05510 0.59 -0.1 254,833 + EX31_RS05510 0.73 +0.4 254,907 - EX31_RS05510 0.80 +0.4 254,907 - EX31_RS05510 0.80 +0.0 254,915 + EX31_RS05510 0.81 -0.9 254,915 + EX31_RS05510 0.81 +1.0 254,915 + EX31_RS05510 0.81 +0.5 254,916 - EX31_RS05510 0.81 -0.3 254,916 - EX31_RS05510 0.81 -0.9 254,916 - EX31_RS05510 0.81 +0.6 254,924 + EX31_RS05510 0.82 -0.8 254,946 + EX31_RS05510 0.84 +0.0 254,946 + EX31_RS05510 0.84 -0.9 255,022 + +0.5 255,023 - +0.0 255,023 - -0.3 255,031 + +0.1 255,031 + -0.6 255,031 + -0.3 255,031 + -0.5 255,031 + -0.6 255,031 + +0.6 255,032 - -1.4 255,032 - -0.1 255,032 - +0.4 255,032 - -0.1 255,032 - +0.1 255,032 - -0.3 255,032 - +1.0 255,054 + -0.1 255,054 + -0.2 255,054 + -0.7 255,054 + +1.0 255,054 + +0.7 255,054 + -0.8 255,054 + +0.0 255,054 + +1.1 255,055 - -0.2 255,055 - -0.4 255,076 - +0.9 255,076 - -0.3 255,079 + +1.2 255,079 + -0.2
Or see this region's nucleotide sequence