Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS01490 and EX31_RS01495 are separated by 16 nucleotides EX31_RS01495 and EX31_RS01500 are separated by 103 nucleotides EX31_RS01500 and EX31_RS01505 are separated by 211 nucleotides
EX31_RS01490: EX31_RS01490 - efflux RND transporter periplasmic adaptor subunit, at 334,700 to 335,866
_RS01490
EX31_RS01495: EX31_RS01495 - multidrug efflux RND transporter permease subunit, at 335,883 to 339,035
_RS01495
EX31_RS01500: EX31_RS01500 - transcriptional regulator, at 339,139 to 339,624
_RS01500
EX31_RS01505: EX31_RS01505 - porin, at 339,836 to 340,438
_RS01505
Position (kb) 335
336
337
338
339
340 Strain fitness (log2 ratio) -2
-1
0
1
2 at 334.902 kb on - strand, within EX31_RS01490 at 334.920 kb on - strand, within EX31_RS01490 at 335.262 kb on + strand, within EX31_RS01490 at 335.263 kb on - strand, within EX31_RS01490 at 335.286 kb on + strand, within EX31_RS01490 at 335.286 kb on + strand, within EX31_RS01490 at 335.287 kb on - strand, within EX31_RS01490 at 335.318 kb on + strand, within EX31_RS01490 at 335.318 kb on + strand, within EX31_RS01490 at 335.318 kb on + strand, within EX31_RS01490 at 335.319 kb on - strand, within EX31_RS01490 at 335.324 kb on + strand, within EX31_RS01490 at 335.471 kb on - strand, within EX31_RS01490 at 335.626 kb on + strand, within EX31_RS01490 at 335.642 kb on - strand, within EX31_RS01490 at 335.651 kb on + strand, within EX31_RS01490 at 335.652 kb on - strand, within EX31_RS01490 at 335.707 kb on - strand, within EX31_RS01490 at 335.708 kb on + strand, within EX31_RS01490 at 335.708 kb on + strand, within EX31_RS01490 at 335.709 kb on - strand, within EX31_RS01490 at 335.719 kb on - strand, within EX31_RS01490 at 335.772 kb on + strand at 335.773 kb on - strand at 335.773 kb on - strand at 335.830 kb on + strand at 335.947 kb on + strand at 335.991 kb on + strand at 336.117 kb on + strand at 336.117 kb on + strand at 336.117 kb on + strand at 336.117 kb on + strand at 336.117 kb on + strand at 336.117 kb on + strand at 336.117 kb on + strand at 336.117 kb on + strand at 336.117 kb on + strand at 336.117 kb on + strand at 336.118 kb on - strand at 336.118 kb on - strand at 336.118 kb on - strand at 336.118 kb on - strand at 336.118 kb on - strand at 336.333 kb on + strand, within EX31_RS01495 at 336.333 kb on + strand, within EX31_RS01495 at 336.333 kb on + strand, within EX31_RS01495 at 336.333 kb on + strand, within EX31_RS01495 at 336.334 kb on - strand, within EX31_RS01495 at 336.334 kb on - strand, within EX31_RS01495 at 336.334 kb on - strand, within EX31_RS01495 at 336.334 kb on - strand, within EX31_RS01495 at 336.357 kb on + strand, within EX31_RS01495 at 336.357 kb on + strand, within EX31_RS01495 at 336.357 kb on + strand, within EX31_RS01495 at 336.358 kb on - strand, within EX31_RS01495 at 336.435 kb on + strand, within EX31_RS01495 at 336.526 kb on + strand, within EX31_RS01495 at 336.527 kb on - strand, within EX31_RS01495 at 336.579 kb on + strand, within EX31_RS01495 at 336.714 kb on + strand, within EX31_RS01495 at 336.714 kb on + strand, within EX31_RS01495 at 336.734 kb on + strand, within EX31_RS01495 at 336.734 kb on + strand, within EX31_RS01495 at 336.734 kb on + strand, within EX31_RS01495 at 336.735 kb on - strand, within EX31_RS01495 at 336.735 kb on - strand, within EX31_RS01495 at 336.740 kb on + strand, within EX31_RS01495 at 337.064 kb on + strand, within EX31_RS01495 at 337.064 kb on + strand, within EX31_RS01495 at 337.065 kb on - strand, within EX31_RS01495 at 337.103 kb on - strand, within EX31_RS01495 at 337.287 kb on + strand, within EX31_RS01495 at 337.288 kb on - strand, within EX31_RS01495 at 337.529 kb on - strand, within EX31_RS01495 at 337.539 kb on + strand, within EX31_RS01495 at 337.618 kb on - strand, within EX31_RS01495 at 337.702 kb on + strand, within EX31_RS01495 at 337.702 kb on + strand, within EX31_RS01495 at 337.703 kb on - strand, within EX31_RS01495 at 337.706 kb on + strand, within EX31_RS01495 at 337.706 kb on + strand, within EX31_RS01495 at 337.707 kb on - strand, within EX31_RS01495 at 337.717 kb on + strand, within EX31_RS01495 at 337.717 kb on + strand, within EX31_RS01495 at 337.824 kb on - strand, within EX31_RS01495 at 337.838 kb on + strand, within EX31_RS01495 at 337.936 kb on - strand, within EX31_RS01495 at 337.944 kb on + strand, within EX31_RS01495 at 337.945 kb on - strand, within EX31_RS01495 at 337.945 kb on - strand, within EX31_RS01495 at 337.946 kb on + strand, within EX31_RS01495 at 337.946 kb on + strand, within EX31_RS01495 at 337.948 kb on + strand, within EX31_RS01495 at 337.948 kb on + strand, within EX31_RS01495 at 337.949 kb on - strand, within EX31_RS01495 at 337.949 kb on - strand, within EX31_RS01495 at 337.995 kb on - strand, within EX31_RS01495 at 337.995 kb on - strand, within EX31_RS01495 at 337.995 kb on - strand, within EX31_RS01495 at 338.151 kb on + strand, within EX31_RS01495 at 338.152 kb on - strand, within EX31_RS01495 at 338.166 kb on + strand, within EX31_RS01495 at 338.166 kb on + strand, within EX31_RS01495 at 338.166 kb on + strand, within EX31_RS01495 at 338.167 kb on - strand, within EX31_RS01495 at 338.168 kb on + strand, within EX31_RS01495 at 338.251 kb on + strand, within EX31_RS01495 at 338.251 kb on + strand, within EX31_RS01495 at 338.252 kb on - strand, within EX31_RS01495 at 338.469 kb on - strand, within EX31_RS01495 at 338.490 kb on + strand, within EX31_RS01495 at 338.575 kb on - strand, within EX31_RS01495 at 338.575 kb on - strand, within EX31_RS01495 at 338.575 kb on - strand, within EX31_RS01495 at 338.717 kb on + strand, within EX31_RS01495 at 338.717 kb on + strand, within EX31_RS01495 at 338.717 kb on + strand, within EX31_RS01495 at 338.717 kb on + strand, within EX31_RS01495 at 338.718 kb on - strand, within EX31_RS01495 at 338.718 kb on - strand, within EX31_RS01495 at 338.718 kb on - strand, within EX31_RS01495 at 338.768 kb on + strand at 339.002 kb on + strand at 339.033 kb on + strand at 339.092 kb on + strand at 339.092 kb on + strand at 339.094 kb on + strand at 339.095 kb on - strand at 339.095 kb on - strand at 339.130 kb on - strand at 339.198 kb on - strand, within EX31_RS01500 at 339.198 kb on - strand, within EX31_RS01500 at 339.198 kb on - strand, within EX31_RS01500 at 339.300 kb on - strand, within EX31_RS01500 at 339.367 kb on - strand, within EX31_RS01500 at 339.367 kb on - strand, within EX31_RS01500 at 339.387 kb on - strand, within EX31_RS01500 at 339.387 kb on - strand, within EX31_RS01500 at 339.533 kb on - strand, within EX31_RS01500 at 339.598 kb on - strand at 339.598 kb on - strand at 339.598 kb on - strand at 339.598 kb on - strand at 339.620 kb on - strand at 339.620 kb on - strand at 339.620 kb on - strand at 339.620 kb on - strand at 339.651 kb on - strand at 339.651 kb on - strand at 339.684 kb on - strand at 339.686 kb on - strand at 339.686 kb on - strand at 339.686 kb on - strand at 339.686 kb on - strand at 339.686 kb on - strand at 339.686 kb on - strand at 339.686 kb on - strand at 339.686 kb on - strand at 339.686 kb on - strand at 339.686 kb on - strand at 339.686 kb on - strand at 339.686 kb on - strand at 339.686 kb on - strand at 339.702 kb on - strand at 339.705 kb on - strand at 339.705 kb on - strand at 339.707 kb on - strand at 339.745 kb on - strand at 339.767 kb on + strand at 339.768 kb on - strand at 339.768 kb on - strand at 339.768 kb on - strand at 339.768 kb on - strand at 339.838 kb on - strand at 339.846 kb on + strand at 339.846 kb on + strand at 339.847 kb on - strand at 339.851 kb on + strand at 339.852 kb on - strand at 339.852 kb on - strand at 339.852 kb on - strand at 339.925 kb on + strand, within EX31_RS01505 at 339.934 kb on - strand, within EX31_RS01505 at 339.942 kb on + strand, within EX31_RS01505 at 339.943 kb on - strand, within EX31_RS01505 at 339.952 kb on + strand, within EX31_RS01505 at 339.967 kb on - strand, within EX31_RS01505 at 339.967 kb on - strand, within EX31_RS01505 at 339.970 kb on - strand, within EX31_RS01505 at 339.990 kb on + strand, within EX31_RS01505 at 339.990 kb on + strand, within EX31_RS01505 at 339.991 kb on - strand, within EX31_RS01505 at 339.991 kb on - strand, within EX31_RS01505 at 339.994 kb on + strand, within EX31_RS01505 at 340.024 kb on + strand, within EX31_RS01505 at 340.025 kb on - strand, within EX31_RS01505 at 340.025 kb on - strand, within EX31_RS01505 at 340.025 kb on - strand, within EX31_RS01505 at 340.025 kb on - strand, within EX31_RS01505
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 334,902 - EX31_RS01490 0.17 -1.6 334,920 - EX31_RS01490 0.19 +0.4 335,262 + EX31_RS01490 0.48 -0.9 335,263 - EX31_RS01490 0.48 +0.2 335,286 + EX31_RS01490 0.50 -0.9 335,286 + EX31_RS01490 0.50 -0.7 335,287 - EX31_RS01490 0.50 +1.6 335,318 + EX31_RS01490 0.53 -2.1 335,318 + EX31_RS01490 0.53 +0.0 335,318 + EX31_RS01490 0.53 -0.4 335,319 - EX31_RS01490 0.53 +1.8 335,324 + EX31_RS01490 0.53 +0.6 335,471 - EX31_RS01490 0.66 +2.0 335,626 + EX31_RS01490 0.79 +1.5 335,642 - EX31_RS01490 0.81 +0.8 335,651 + EX31_RS01490 0.81 +0.4 335,652 - EX31_RS01490 0.82 -0.6 335,707 - EX31_RS01490 0.86 +1.2 335,708 + EX31_RS01490 0.86 +0.4 335,708 + EX31_RS01490 0.86 +1.5 335,709 - EX31_RS01490 0.86 +1.4 335,719 - EX31_RS01490 0.87 -1.3 335,772 + +2.0 335,773 - +0.2 335,773 - +2.0 335,830 + +1.0 335,947 + -0.1 335,991 + -0.6 336,117 + +1.6 336,117 + -0.8 336,117 + -1.0 336,117 + +0.2 336,117 + -0.1 336,117 + -1.3 336,117 + +0.6 336,117 + -2.1 336,117 + +0.2 336,117 + +1.4 336,118 - +0.1 336,118 - -0.4 336,118 - +0.9 336,118 - +1.6 336,118 - +0.2 336,333 + EX31_RS01495 0.14 +0.3 336,333 + EX31_RS01495 0.14 +1.0 336,333 + EX31_RS01495 0.14 +2.0 336,333 + EX31_RS01495 0.14 +1.0 336,334 - EX31_RS01495 0.14 +0.2 336,334 - EX31_RS01495 0.14 -0.4 336,334 - EX31_RS01495 0.14 -0.1 336,334 - EX31_RS01495 0.14 +0.6 336,357 + EX31_RS01495 0.15 +1.5 336,357 + EX31_RS01495 0.15 +0.2 336,357 + EX31_RS01495 0.15 -0.1 336,358 - EX31_RS01495 0.15 +0.4 336,435 + EX31_RS01495 0.18 -1.6 336,526 + EX31_RS01495 0.20 -0.6 336,527 - EX31_RS01495 0.20 +0.7 336,579 + EX31_RS01495 0.22 +0.0 336,714 + EX31_RS01495 0.26 -0.3 336,714 + EX31_RS01495 0.26 +1.0 336,734 + EX31_RS01495 0.27 +0.4 336,734 + EX31_RS01495 0.27 +0.4 336,734 + EX31_RS01495 0.27 +2.4 336,735 - EX31_RS01495 0.27 +0.6 336,735 - EX31_RS01495 0.27 +0.1 336,740 + EX31_RS01495 0.27 -0.6 337,064 + EX31_RS01495 0.37 +1.2 337,064 + EX31_RS01495 0.37 +0.4 337,065 - EX31_RS01495 0.37 +1.1 337,103 - EX31_RS01495 0.39 +1.0 337,287 + EX31_RS01495 0.45 -0.2 337,288 - EX31_RS01495 0.45 +2.0 337,529 - EX31_RS01495 0.52 -0.6 337,539 + EX31_RS01495 0.53 +0.0 337,618 - EX31_RS01495 0.55 -0.0 337,702 + EX31_RS01495 0.58 +0.4 337,702 + EX31_RS01495 0.58 +0.6 337,703 - EX31_RS01495 0.58 -0.4 337,706 + EX31_RS01495 0.58 -2.4 337,706 + EX31_RS01495 0.58 +0.6 337,707 - EX31_RS01495 0.58 -0.2 337,717 + EX31_RS01495 0.58 +0.0 337,717 + EX31_RS01495 0.58 -0.1 337,824 - EX31_RS01495 0.62 +1.6 337,838 + EX31_RS01495 0.62 -0.3 337,936 - EX31_RS01495 0.65 +0.4 337,944 + EX31_RS01495 0.65 +0.8 337,945 - EX31_RS01495 0.65 -1.4 337,945 - EX31_RS01495 0.65 +0.2 337,946 + EX31_RS01495 0.65 +0.5 337,946 + EX31_RS01495 0.65 -0.2 337,948 + EX31_RS01495 0.65 +1.3 337,948 + EX31_RS01495 0.65 -0.0 337,949 - EX31_RS01495 0.66 +1.7 337,949 - EX31_RS01495 0.66 +1.0 337,995 - EX31_RS01495 0.67 +0.6 337,995 - EX31_RS01495 0.67 +0.2 337,995 - EX31_RS01495 0.67 -1.4 338,151 + EX31_RS01495 0.72 -1.7 338,152 - EX31_RS01495 0.72 -0.2 338,166 + EX31_RS01495 0.72 +1.6 338,166 + EX31_RS01495 0.72 +1.4 338,166 + EX31_RS01495 0.72 +0.3 338,167 - EX31_RS01495 0.72 -0.3 338,168 + EX31_RS01495 0.72 +2.0 338,251 + EX31_RS01495 0.75 +0.7 338,251 + EX31_RS01495 0.75 -0.2 338,252 - EX31_RS01495 0.75 -1.3 338,469 - EX31_RS01495 0.82 +0.2 338,490 + EX31_RS01495 0.83 +1.0 338,575 - EX31_RS01495 0.85 +1.0 338,575 - EX31_RS01495 0.85 -2.2 338,575 - EX31_RS01495 0.85 +0.4 338,717 + EX31_RS01495 0.90 +0.9 338,717 + EX31_RS01495 0.90 +0.2 338,717 + EX31_RS01495 0.90 +1.6 338,717 + EX31_RS01495 0.90 +0.0 338,718 - EX31_RS01495 0.90 +0.8 338,718 - EX31_RS01495 0.90 +0.4 338,718 - EX31_RS01495 0.90 +0.6 338,768 + -1.0 339,002 + +0.6 339,033 + +0.3 339,092 + +1.6 339,092 + -0.3 339,094 + +0.2 339,095 - +0.0 339,095 - +0.4 339,130 - +0.3 339,198 - EX31_RS01500 0.12 +0.5 339,198 - EX31_RS01500 0.12 +0.0 339,198 - EX31_RS01500 0.12 +0.0 339,300 - EX31_RS01500 0.33 -0.8 339,367 - EX31_RS01500 0.47 -0.6 339,367 - EX31_RS01500 0.47 -0.6 339,387 - EX31_RS01500 0.51 +0.4 339,387 - EX31_RS01500 0.51 -0.9 339,533 - EX31_RS01500 0.81 -1.9 339,598 - -0.3 339,598 - -0.6 339,598 - -0.8 339,598 - -1.4 339,620 - -0.7 339,620 - -0.1 339,620 - +0.2 339,620 - +1.2 339,651 - +0.8 339,651 - -1.8 339,684 - +2.0 339,686 - +0.1 339,686 - +0.6 339,686 - +0.7 339,686 - -0.3 339,686 - +1.0 339,686 - -1.3 339,686 - -0.4 339,686 - +0.8 339,686 - -0.7 339,686 - +0.7 339,686 - -0.1 339,686 - -0.7 339,686 - +1.4 339,702 - +0.0 339,705 - -0.4 339,705 - +0.2 339,707 - +0.3 339,745 - +1.0 339,767 + -0.6 339,768 - +0.6 339,768 - -1.6 339,768 - -2.2 339,768 - +0.5 339,838 - +0.8 339,846 + -0.7 339,846 + +1.0 339,847 - +1.0 339,851 + +0.0 339,852 - -0.2 339,852 - -2.1 339,852 - +0.9 339,925 + EX31_RS01505 0.15 +0.0 339,934 - EX31_RS01505 0.16 -0.2 339,942 + EX31_RS01505 0.18 +0.9 339,943 - EX31_RS01505 0.18 +1.0 339,952 + EX31_RS01505 0.19 +0.2 339,967 - EX31_RS01505 0.22 -0.8 339,967 - EX31_RS01505 0.22 +1.0 339,970 - EX31_RS01505 0.22 -0.1 339,990 + EX31_RS01505 0.26 +0.8 339,990 + EX31_RS01505 0.26 +0.3 339,991 - EX31_RS01505 0.26 +0.6 339,991 - EX31_RS01505 0.26 -0.4 339,994 + EX31_RS01505 0.26 -1.1 340,024 + EX31_RS01505 0.31 +0.2 340,025 - EX31_RS01505 0.31 -0.8 340,025 - EX31_RS01505 0.31 +0.3 340,025 - EX31_RS01505 0.31 +0.9 340,025 - EX31_RS01505 0.31 -0.3
Or see this region's nucleotide sequence