Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS00110 and EX31_RS00115 are separated by 88 nucleotides EX31_RS00115 and EX31_RS00120 overlap by 8 nucleotides EX31_RS00120 and EX31_RS00125 are separated by 11 nucleotides
EX31_RS00110: EX31_RS00110 - ABC transporter permease, at 24,490 to 25,317
_RS00110
EX31_RS00115: EX31_RS00115 - SDR family oxidoreductase, at 25,406 to 26,149
_RS00115
EX31_RS00120: EX31_RS00120 - phosphotransferase, at 26,142 to 27,191
_RS00120
EX31_RS00125: EX31_RS00125 - aminotransferase class III-fold pyridoxal phosphate-dependent enzyme, at 27,203 to 28,462
_RS00125
Position (kb) 26
27
28 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 25.341 kb on - strand at 25.570 kb on + strand, within EX31_RS00115 at 25.571 kb on - strand, within EX31_RS00115 at 25.672 kb on + strand, within EX31_RS00115 at 25.673 kb on - strand, within EX31_RS00115 at 25.830 kb on + strand, within EX31_RS00115 at 25.877 kb on + strand, within EX31_RS00115 at 25.877 kb on + strand, within EX31_RS00115 at 25.877 kb on + strand, within EX31_RS00115 at 25.878 kb on - strand, within EX31_RS00115 at 25.878 kb on - strand, within EX31_RS00115 at 25.888 kb on + strand, within EX31_RS00115 at 25.954 kb on - strand, within EX31_RS00115 at 25.981 kb on + strand, within EX31_RS00115 at 26.102 kb on + strand at 26.102 kb on + strand at 26.102 kb on + strand at 26.103 kb on - strand at 26.103 kb on - strand at 26.121 kb on + strand at 26.121 kb on + strand at 26.121 kb on + strand at 26.122 kb on - strand at 26.122 kb on - strand at 26.208 kb on - strand at 26.304 kb on + strand, within EX31_RS00120 at 26.304 kb on + strand, within EX31_RS00120 at 26.304 kb on + strand, within EX31_RS00120 at 26.304 kb on + strand, within EX31_RS00120 at 26.304 kb on + strand, within EX31_RS00120 at 26.305 kb on - strand, within EX31_RS00120 at 26.490 kb on + strand, within EX31_RS00120 at 26.490 kb on + strand, within EX31_RS00120 at 26.490 kb on + strand, within EX31_RS00120 at 26.491 kb on - strand, within EX31_RS00120 at 26.591 kb on + strand, within EX31_RS00120 at 26.929 kb on - strand, within EX31_RS00120 at 26.952 kb on + strand, within EX31_RS00120 at 26.954 kb on + strand, within EX31_RS00120 at 27.079 kb on - strand, within EX31_RS00120 at 27.079 kb on - strand, within EX31_RS00120 at 27.080 kb on + strand, within EX31_RS00120 at 27.080 kb on + strand, within EX31_RS00120 at 27.081 kb on - strand, within EX31_RS00120 at 27.157 kb on + strand at 27.158 kb on - strand at 27.158 kb on - strand at 27.189 kb on + strand at 27.189 kb on + strand at 27.189 kb on + strand at 27.190 kb on - strand at 27.200 kb on + strand at 27.200 kb on + strand at 27.200 kb on + strand at 27.201 kb on - strand at 27.201 kb on - strand at 27.202 kb on + strand at 27.202 kb on + strand at 27.202 kb on + strand at 27.202 kb on + strand at 27.202 kb on + strand at 27.203 kb on - strand at 27.203 kb on - strand at 27.203 kb on - strand at 27.203 kb on - strand at 27.203 kb on - strand at 27.203 kb on - strand at 27.312 kb on + strand at 27.312 kb on + strand at 27.312 kb on + strand at 27.313 kb on - strand at 27.313 kb on - strand at 27.314 kb on + strand at 27.314 kb on + strand at 27.315 kb on - strand at 27.315 kb on - strand at 27.315 kb on - strand at 27.315 kb on - strand at 27.315 kb on - strand at 27.348 kb on - strand, within EX31_RS00125 at 27.348 kb on - strand, within EX31_RS00125 at 27.348 kb on - strand, within EX31_RS00125 at 27.352 kb on + strand, within EX31_RS00125 at 27.353 kb on - strand, within EX31_RS00125 at 27.568 kb on + strand, within EX31_RS00125 at 27.720 kb on + strand, within EX31_RS00125 at 27.720 kb on + strand, within EX31_RS00125 at 27.820 kb on - strand, within EX31_RS00125 at 27.820 kb on - strand, within EX31_RS00125
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 25,341 - -0.3 25,570 + EX31_RS00115 0.22 +0.4 25,571 - EX31_RS00115 0.22 +0.0 25,672 + EX31_RS00115 0.36 -1.6 25,673 - EX31_RS00115 0.36 +0.3 25,830 + EX31_RS00115 0.57 +0.6 25,877 + EX31_RS00115 0.63 +0.8 25,877 + EX31_RS00115 0.63 +1.0 25,877 + EX31_RS00115 0.63 -0.8 25,878 - EX31_RS00115 0.63 -0.8 25,878 - EX31_RS00115 0.63 +0.9 25,888 + EX31_RS00115 0.65 +0.6 25,954 - EX31_RS00115 0.74 -0.3 25,981 + EX31_RS00115 0.77 -0.4 26,102 + +0.9 26,102 + +0.0 26,102 + -0.8 26,103 - -1.3 26,103 - -1.0 26,121 + -0.3 26,121 + -0.9 26,121 + +0.2 26,122 - -0.5 26,122 - -1.4 26,208 - +0.7 26,304 + EX31_RS00120 0.15 +0.8 26,304 + EX31_RS00120 0.15 +1.3 26,304 + EX31_RS00120 0.15 -0.6 26,304 + EX31_RS00120 0.15 -0.4 26,304 + EX31_RS00120 0.15 +0.6 26,305 - EX31_RS00120 0.16 +0.6 26,490 + EX31_RS00120 0.33 -0.3 26,490 + EX31_RS00120 0.33 +0.6 26,490 + EX31_RS00120 0.33 -0.5 26,491 - EX31_RS00120 0.33 +0.6 26,591 + EX31_RS00120 0.43 -1.0 26,929 - EX31_RS00120 0.75 +1.0 26,952 + EX31_RS00120 0.77 +1.1 26,954 + EX31_RS00120 0.77 +2.4 27,079 - EX31_RS00120 0.89 +0.6 27,079 - EX31_RS00120 0.89 +0.4 27,080 + EX31_RS00120 0.89 +0.7 27,080 + EX31_RS00120 0.89 +0.8 27,081 - EX31_RS00120 0.89 +1.0 27,157 + +0.3 27,158 - +0.6 27,158 - +0.5 27,189 + +0.6 27,189 + +3.3 27,189 + +3.2 27,190 - +0.6 27,200 + -1.0 27,200 + -1.3 27,200 + +0.9 27,201 - +0.5 27,201 - +1.5 27,202 + -0.5 27,202 + +2.0 27,202 + +0.4 27,202 + +0.8 27,202 + -0.4 27,203 - -0.3 27,203 - -0.1 27,203 - -0.6 27,203 - +1.4 27,203 - +1.3 27,203 - +0.8 27,312 + -0.7 27,312 + +1.1 27,312 + -0.8 27,313 - -0.6 27,313 - +0.6 27,314 + -0.6 27,314 + +0.3 27,315 - +0.0 27,315 - -0.4 27,315 - +0.2 27,315 - +0.3 27,315 - -0.8 27,348 - EX31_RS00125 0.12 -0.3 27,348 - EX31_RS00125 0.12 -1.1 27,348 - EX31_RS00125 0.12 -1.8 27,352 + EX31_RS00125 0.12 -0.7 27,353 - EX31_RS00125 0.12 +0.2 27,568 + EX31_RS00125 0.29 -1.4 27,720 + EX31_RS00125 0.41 -1.4 27,720 + EX31_RS00125 0.41 +0.4 27,820 - EX31_RS00125 0.49 -0.3 27,820 - EX31_RS00125 0.49 +0.4
Or see this region's nucleotide sequence