Experiment: R2A with Nalidixic 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS25370 and CA265_RS25375 overlap by 31 nucleotides CA265_RS25375 and CA265_RS25380 are separated by 29 nucleotides CA265_RS25380 and CA265_RS25385 overlap by 19 nucleotides CA265_RS25385 and CA265_RS25390 are separated by 16 nucleotides CA265_RS25390 and CA265_RS25395 are separated by 31 nucleotides
CA265_RS25370: CA265_RS25370 - glycosyl transferase family 2, at 6,165,058 to 6,166,155
_RS25370
CA265_RS25375: CA265_RS25375 - hypothetical protein, at 6,166,125 to 6,166,799
_RS25375
CA265_RS25380: CA265_RS25380 - hypothetical protein, at 6,166,829 to 6,167,038
_RS25380
CA265_RS25385: CA265_RS25385 - Txe/YoeB family addiction module toxin, at 6,167,020 to 6,167,301
_RS25385
CA265_RS25390: CA265_RS25390 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 6,167,318 to 6,168,169
_RS25390
CA265_RS25395: CA265_RS25395 - cysteine hydrolase, at 6,168,201 to 6,168,743
_RS25395
Position (kb)
6167
6168 Strain fitness (log2 ratio)
-1
0
1
2
3 at 6166.077 kb on - strand at 6166.090 kb on - strand at 6166.153 kb on + strand at 6166.181 kb on - strand at 6166.239 kb on + strand, within CA265_RS25375 at 6166.324 kb on + strand, within CA265_RS25375 at 6166.342 kb on + strand, within CA265_RS25375 at 6166.400 kb on + strand, within CA265_RS25375 at 6166.408 kb on - strand, within CA265_RS25375 at 6166.411 kb on - strand, within CA265_RS25375 at 6166.454 kb on - strand, within CA265_RS25375 at 6166.553 kb on + strand, within CA265_RS25375 at 6166.562 kb on - strand, within CA265_RS25375 at 6166.687 kb on - strand, within CA265_RS25375 at 6166.716 kb on + strand, within CA265_RS25375 at 6166.717 kb on - strand, within CA265_RS25375 at 6166.748 kb on - strand at 6166.748 kb on - strand at 6166.766 kb on - strand at 6167.036 kb on + strand at 6167.055 kb on + strand, within CA265_RS25385 at 6167.091 kb on + strand, within CA265_RS25385 at 6167.132 kb on + strand, within CA265_RS25385 at 6167.132 kb on + strand, within CA265_RS25385 at 6167.231 kb on + strand, within CA265_RS25385 at 6167.231 kb on + strand, within CA265_RS25385 at 6167.233 kb on + strand, within CA265_RS25385 at 6167.256 kb on + strand, within CA265_RS25385 at 6168.186 kb on - strand at 6168.188 kb on - strand at 6168.191 kb on + strand at 6168.220 kb on + strand at 6168.290 kb on - strand, within CA265_RS25395 at 6168.294 kb on - strand, within CA265_RS25395
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nalidixic 0.008 mg/ml remove 6,166,077 - -0.1 6,166,090 - +0.9 6,166,153 + +3.7 6,166,181 - +0.6 6,166,239 + CA265_RS25375 0.17 +0.5 6,166,324 + CA265_RS25375 0.29 -0.1 6,166,342 + CA265_RS25375 0.32 -0.3 6,166,400 + CA265_RS25375 0.41 +0.5 6,166,408 - CA265_RS25375 0.42 +0.6 6,166,411 - CA265_RS25375 0.42 +0.5 6,166,454 - CA265_RS25375 0.49 +1.0 6,166,553 + CA265_RS25375 0.63 -1.0 6,166,562 - CA265_RS25375 0.65 +1.4 6,166,687 - CA265_RS25375 0.83 +0.1 6,166,716 + CA265_RS25375 0.88 +0.8 6,166,717 - CA265_RS25375 0.88 +1.1 6,166,748 - +1.1 6,166,748 - -0.2 6,166,766 - +0.4 6,167,036 + -0.6 6,167,055 + CA265_RS25385 0.12 +0.0 6,167,091 + CA265_RS25385 0.25 -0.3 6,167,132 + CA265_RS25385 0.40 -0.0 6,167,132 + CA265_RS25385 0.40 +0.1 6,167,231 + CA265_RS25385 0.75 -0.5 6,167,231 + CA265_RS25385 0.75 -0.8 6,167,233 + CA265_RS25385 0.76 +0.8 6,167,256 + CA265_RS25385 0.84 -0.1 6,168,186 - +1.4 6,168,188 - +0.8 6,168,191 + +0.4 6,168,220 + +0.6 6,168,290 - CA265_RS25395 0.16 +0.7 6,168,294 - CA265_RS25395 0.17 +0.8
Or see this region's nucleotide sequence