Experiment: R2A with Nalidixic 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS20715 and CA265_RS20720 are separated by 5 nucleotides CA265_RS20720 and CA265_RS20725 overlap by 4 nucleotides CA265_RS20725 and CA265_RS20730 overlap by 4 nucleotides
CA265_RS20715: CA265_RS20715 - conjugal transfer protein TraG, at 5,119,680 to 5,122,130
_RS20715
CA265_RS20720: CA265_RS20720 - hypothetical protein, at 5,122,136 to 5,122,798
_RS20720
CA265_RS20725: CA265_RS20725 - hypothetical protein, at 5,122,795 to 5,123,451
_RS20725
CA265_RS20730: CA265_RS20730 - hypothetical protein, at 5,123,448 to 5,124,113
_RS20730
Position (kb)
5122
5123 Strain fitness (log2 ratio)
-2
-1
0
1 at 5121.249 kb on + strand, within CA265_RS20715 at 5121.300 kb on - strand, within CA265_RS20715 at 5121.422 kb on + strand, within CA265_RS20715 at 5121.478 kb on - strand, within CA265_RS20715 at 5121.534 kb on - strand, within CA265_RS20715 at 5121.534 kb on - strand, within CA265_RS20715 at 5121.544 kb on + strand, within CA265_RS20715 at 5121.544 kb on + strand, within CA265_RS20715 at 5121.545 kb on - strand, within CA265_RS20715 at 5121.545 kb on - strand, within CA265_RS20715 at 5121.545 kb on - strand, within CA265_RS20715 at 5121.545 kb on - strand, within CA265_RS20715 at 5121.549 kb on - strand, within CA265_RS20715 at 5121.643 kb on + strand, within CA265_RS20715 at 5121.648 kb on + strand, within CA265_RS20715 at 5121.649 kb on - strand, within CA265_RS20715 at 5121.743 kb on + strand, within CA265_RS20715 at 5121.744 kb on - strand, within CA265_RS20715 at 5121.903 kb on + strand at 5121.931 kb on + strand at 5121.933 kb on + strand at 5121.934 kb on - strand at 5121.955 kb on - strand at 5122.009 kb on - strand at 5122.119 kb on - strand at 5122.132 kb on + strand at 5122.135 kb on + strand at 5122.154 kb on - strand at 5122.154 kb on - strand at 5122.200 kb on - strand at 5122.234 kb on - strand, within CA265_RS20720 at 5122.355 kb on + strand, within CA265_RS20720 at 5122.356 kb on - strand, within CA265_RS20720 at 5122.356 kb on - strand, within CA265_RS20720 at 5122.356 kb on - strand, within CA265_RS20720 at 5122.357 kb on + strand, within CA265_RS20720 at 5122.415 kb on + strand, within CA265_RS20720 at 5122.416 kb on - strand, within CA265_RS20720 at 5122.433 kb on - strand, within CA265_RS20720 at 5122.433 kb on - strand, within CA265_RS20720 at 5122.434 kb on + strand, within CA265_RS20720 at 5122.435 kb on - strand, within CA265_RS20720 at 5122.545 kb on + strand, within CA265_RS20720 at 5122.670 kb on + strand, within CA265_RS20720 at 5122.670 kb on + strand, within CA265_RS20720 at 5122.682 kb on + strand, within CA265_RS20720 at 5122.682 kb on + strand, within CA265_RS20720 at 5122.683 kb on - strand, within CA265_RS20720 at 5122.736 kb on - strand at 5122.747 kb on + strand at 5122.747 kb on + strand at 5122.809 kb on + strand at 5122.810 kb on - strand at 5122.810 kb on - strand at 5122.815 kb on - strand at 5122.835 kb on + strand at 5123.079 kb on - strand, within CA265_RS20725 at 5123.089 kb on - strand, within CA265_RS20725 at 5123.121 kb on + strand, within CA265_RS20725 at 5123.122 kb on - strand, within CA265_RS20725 at 5123.122 kb on - strand, within CA265_RS20725 at 5123.187 kb on + strand, within CA265_RS20725 at 5123.187 kb on + strand, within CA265_RS20725 at 5123.201 kb on - strand at 5123.256 kb on + strand, within CA265_RS20725 at 5123.292 kb on + strand, within CA265_RS20725 at 5123.463 kb on - strand at 5123.463 kb on - strand at 5123.468 kb on - strand at 5123.477 kb on - strand at 5123.593 kb on - strand, within CA265_RS20730 at 5123.601 kb on - strand, within CA265_RS20730 at 5123.624 kb on + strand, within CA265_RS20730 at 5123.625 kb on - strand, within CA265_RS20730 at 5123.702 kb on - strand, within CA265_RS20730 at 5123.748 kb on - strand, within CA265_RS20730 at 5123.748 kb on - strand, within CA265_RS20730 at 5123.750 kb on - strand, within CA265_RS20730 at 5123.750 kb on - strand, within CA265_RS20730
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nalidixic 0.008 mg/ml remove 5,121,249 + CA265_RS20715 0.64 -0.4 5,121,300 - CA265_RS20715 0.66 -0.3 5,121,422 + CA265_RS20715 0.71 -0.3 5,121,478 - CA265_RS20715 0.73 -0.2 5,121,534 - CA265_RS20715 0.76 -0.8 5,121,534 - CA265_RS20715 0.76 -0.0 5,121,544 + CA265_RS20715 0.76 -0.0 5,121,544 + CA265_RS20715 0.76 -1.2 5,121,545 - CA265_RS20715 0.76 +0.7 5,121,545 - CA265_RS20715 0.76 -0.8 5,121,545 - CA265_RS20715 0.76 -0.6 5,121,545 - CA265_RS20715 0.76 +0.9 5,121,549 - CA265_RS20715 0.76 -0.3 5,121,643 + CA265_RS20715 0.80 -0.4 5,121,648 + CA265_RS20715 0.80 +0.4 5,121,649 - CA265_RS20715 0.80 +1.4 5,121,743 + CA265_RS20715 0.84 -0.7 5,121,744 - CA265_RS20715 0.84 +0.9 5,121,903 + -1.0 5,121,931 + -0.4 5,121,933 + -1.3 5,121,934 - -0.0 5,121,955 - +0.7 5,122,009 - -0.8 5,122,119 - -0.5 5,122,132 + -0.3 5,122,135 + -1.3 5,122,154 - -0.6 5,122,154 - -0.2 5,122,200 - -0.6 5,122,234 - CA265_RS20720 0.15 +0.2 5,122,355 + CA265_RS20720 0.33 -0.3 5,122,356 - CA265_RS20720 0.33 -0.8 5,122,356 - CA265_RS20720 0.33 -0.7 5,122,356 - CA265_RS20720 0.33 -2.2 5,122,357 + CA265_RS20720 0.33 -0.7 5,122,415 + CA265_RS20720 0.42 +0.7 5,122,416 - CA265_RS20720 0.42 -0.3 5,122,433 - CA265_RS20720 0.45 +0.3 5,122,433 - CA265_RS20720 0.45 +0.6 5,122,434 + CA265_RS20720 0.45 +0.1 5,122,435 - CA265_RS20720 0.45 -0.5 5,122,545 + CA265_RS20720 0.62 +0.5 5,122,670 + CA265_RS20720 0.81 -0.1 5,122,670 + CA265_RS20720 0.81 -0.6 5,122,682 + CA265_RS20720 0.82 -1.8 5,122,682 + CA265_RS20720 0.82 -0.8 5,122,683 - CA265_RS20720 0.83 -1.1 5,122,736 - -0.6 5,122,747 + +0.2 5,122,747 + -0.8 5,122,809 + -1.6 5,122,810 - -0.1 5,122,810 - -1.2 5,122,815 - -1.2 5,122,835 + -0.9 5,123,079 - CA265_RS20725 0.43 -1.1 5,123,089 - CA265_RS20725 0.45 -2.5 5,123,121 + CA265_RS20725 0.50 -0.4 5,123,122 - CA265_RS20725 0.50 -0.1 5,123,122 - CA265_RS20725 0.50 -0.3 5,123,187 + CA265_RS20725 0.60 +0.0 5,123,187 + CA265_RS20725 0.60 +0.1 5,123,201 - -0.3 5,123,256 + CA265_RS20725 0.70 -1.0 5,123,292 + CA265_RS20725 0.76 -0.3 5,123,463 - +0.2 5,123,463 - +1.0 5,123,468 - +1.0 5,123,477 - -0.4 5,123,593 - CA265_RS20730 0.22 +0.7 5,123,601 - CA265_RS20730 0.23 -0.4 5,123,624 + CA265_RS20730 0.26 -0.0 5,123,625 - CA265_RS20730 0.27 -0.4 5,123,702 - CA265_RS20730 0.38 -0.2 5,123,748 - CA265_RS20730 0.45 -0.1 5,123,748 - CA265_RS20730 0.45 -1.6 5,123,750 - CA265_RS20730 0.45 -0.7 5,123,750 - CA265_RS20730 0.45 -0.7
Or see this region's nucleotide sequence