Experiment: R2A with Nalidixic 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS19530 and CA265_RS19535 are separated by 29 nucleotides CA265_RS19535 and CA265_RS19540 are separated by 246 nucleotides CA265_RS19540 and CA265_RS19545 are separated by 222 nucleotides
CA265_RS19530: CA265_RS19530 - asparaginase, at 4,842,449 to 4,843,291
_RS19530
CA265_RS19535: CA265_RS19535 - hypothetical protein, at 4,843,321 to 4,844,010
_RS19535
CA265_RS19540: CA265_RS19540 - tail-specific protease, at 4,844,257 to 4,846,446
_RS19540
CA265_RS19545: CA265_RS19545 - protease, at 4,846,669 to 4,847,610
_RS19545
Position (kb) 4844
4845
4846
4847 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4843.271 kb on - strand at 4843.282 kb on - strand at 4843.302 kb on - strand at 4843.364 kb on + strand at 4843.364 kb on + strand at 4843.371 kb on - strand at 4843.389 kb on + strand at 4843.410 kb on + strand, within CA265_RS19535 at 4843.424 kb on + strand, within CA265_RS19535 at 4843.425 kb on - strand, within CA265_RS19535 at 4843.429 kb on - strand, within CA265_RS19535 at 4843.429 kb on - strand, within CA265_RS19535 at 4843.527 kb on - strand, within CA265_RS19535 at 4843.552 kb on - strand, within CA265_RS19535 at 4843.700 kb on + strand, within CA265_RS19535 at 4843.711 kb on + strand, within CA265_RS19535 at 4843.711 kb on + strand, within CA265_RS19535 at 4843.714 kb on - strand, within CA265_RS19535 at 4843.714 kb on - strand, within CA265_RS19535 at 4843.744 kb on - strand, within CA265_RS19535 at 4843.750 kb on + strand, within CA265_RS19535 at 4843.786 kb on - strand, within CA265_RS19535 at 4843.802 kb on + strand, within CA265_RS19535 at 4843.835 kb on + strand, within CA265_RS19535 at 4843.936 kb on + strand, within CA265_RS19535 at 4843.936 kb on + strand, within CA265_RS19535 at 4843.937 kb on - strand, within CA265_RS19535 at 4843.937 kb on - strand, within CA265_RS19535 at 4844.042 kb on - strand at 4844.056 kb on - strand at 4844.056 kb on - strand at 4844.058 kb on - strand at 4844.131 kb on + strand at 4844.131 kb on + strand at 4844.143 kb on - strand at 4844.195 kb on - strand at 4844.195 kb on - strand at 4844.235 kb on + strand at 4844.245 kb on - strand at 4844.246 kb on + strand at 4844.246 kb on + strand at 4844.371 kb on - strand at 4844.440 kb on - strand at 4844.490 kb on + strand, within CA265_RS19540 at 4844.494 kb on + strand, within CA265_RS19540 at 4844.495 kb on - strand, within CA265_RS19540 at 4844.497 kb on - strand, within CA265_RS19540 at 4844.566 kb on + strand, within CA265_RS19540 at 4844.566 kb on + strand, within CA265_RS19540 at 4844.566 kb on + strand, within CA265_RS19540 at 4844.567 kb on - strand, within CA265_RS19540 at 4844.568 kb on + strand, within CA265_RS19540 at 4844.568 kb on + strand, within CA265_RS19540 at 4844.569 kb on - strand, within CA265_RS19540 at 4844.574 kb on - strand, within CA265_RS19540 at 4844.574 kb on - strand, within CA265_RS19540 at 4844.601 kb on - strand, within CA265_RS19540 at 4844.601 kb on - strand, within CA265_RS19540 at 4844.622 kb on + strand, within CA265_RS19540 at 4844.630 kb on - strand, within CA265_RS19540 at 4844.660 kb on - strand, within CA265_RS19540 at 4844.660 kb on - strand, within CA265_RS19540 at 4844.710 kb on + strand, within CA265_RS19540 at 4844.710 kb on + strand, within CA265_RS19540 at 4844.710 kb on + strand, within CA265_RS19540 at 4844.785 kb on - strand, within CA265_RS19540 at 4844.789 kb on - strand, within CA265_RS19540 at 4844.850 kb on + strand, within CA265_RS19540 at 4844.949 kb on + strand, within CA265_RS19540 at 4844.950 kb on - strand, within CA265_RS19540 at 4844.950 kb on - strand, within CA265_RS19540 at 4844.994 kb on + strand, within CA265_RS19540 at 4844.994 kb on + strand, within CA265_RS19540 at 4844.995 kb on - strand, within CA265_RS19540 at 4845.035 kb on + strand, within CA265_RS19540 at 4845.036 kb on - strand, within CA265_RS19540 at 4845.088 kb on - strand, within CA265_RS19540 at 4845.101 kb on + strand, within CA265_RS19540 at 4845.102 kb on - strand, within CA265_RS19540 at 4845.102 kb on - strand, within CA265_RS19540 at 4845.120 kb on + strand, within CA265_RS19540 at 4845.120 kb on + strand, within CA265_RS19540 at 4845.152 kb on + strand, within CA265_RS19540 at 4845.153 kb on - strand, within CA265_RS19540 at 4845.185 kb on + strand, within CA265_RS19540 at 4845.185 kb on + strand, within CA265_RS19540 at 4845.186 kb on - strand, within CA265_RS19540 at 4845.278 kb on + strand, within CA265_RS19540 at 4845.279 kb on - strand, within CA265_RS19540 at 4845.348 kb on + strand, within CA265_RS19540 at 4845.369 kb on + strand, within CA265_RS19540 at 4845.388 kb on + strand, within CA265_RS19540 at 4845.388 kb on + strand, within CA265_RS19540 at 4845.389 kb on - strand, within CA265_RS19540 at 4845.389 kb on - strand, within CA265_RS19540 at 4845.488 kb on + strand, within CA265_RS19540 at 4845.501 kb on - strand, within CA265_RS19540 at 4845.571 kb on - strand, within CA265_RS19540 at 4845.642 kb on - strand, within CA265_RS19540 at 4845.671 kb on + strand, within CA265_RS19540 at 4845.717 kb on - strand, within CA265_RS19540 at 4845.735 kb on + strand, within CA265_RS19540 at 4845.738 kb on + strand, within CA265_RS19540 at 4845.738 kb on + strand, within CA265_RS19540 at 4845.763 kb on - strand, within CA265_RS19540 at 4845.836 kb on + strand, within CA265_RS19540 at 4845.844 kb on - strand, within CA265_RS19540 at 4845.861 kb on + strand at 4845.862 kb on - strand, within CA265_RS19540 at 4846.009 kb on - strand, within CA265_RS19540 at 4846.214 kb on + strand, within CA265_RS19540 at 4846.408 kb on + strand at 4846.409 kb on - strand at 4846.409 kb on - strand at 4846.444 kb on + strand at 4846.455 kb on - strand at 4846.632 kb on + strand at 4846.632 kb on + strand at 4846.705 kb on + strand at 4846.828 kb on + strand, within CA265_RS19545 at 4846.878 kb on + strand, within CA265_RS19545 at 4846.977 kb on + strand, within CA265_RS19545 at 4846.978 kb on - strand, within CA265_RS19545 at 4847.010 kb on + strand, within CA265_RS19545 at 4847.086 kb on - strand, within CA265_RS19545 at 4847.103 kb on + strand, within CA265_RS19545 at 4847.103 kb on + strand, within CA265_RS19545 at 4847.106 kb on + strand, within CA265_RS19545 at 4847.234 kb on + strand, within CA265_RS19545 at 4847.234 kb on + strand, within CA265_RS19545 at 4847.234 kb on + strand, within CA265_RS19545 at 4847.235 kb on - strand, within CA265_RS19545 at 4847.278 kb on + strand, within CA265_RS19545 at 4847.293 kb on + strand, within CA265_RS19545 at 4847.293 kb on + strand, within CA265_RS19545 at 4847.301 kb on + strand, within CA265_RS19545 at 4847.348 kb on + strand, within CA265_RS19545 at 4847.349 kb on - strand, within CA265_RS19545 at 4847.354 kb on - strand, within CA265_RS19545 at 4847.369 kb on + strand, within CA265_RS19545
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nalidixic 0.008 mg/ml remove 4,843,271 - -0.3 4,843,282 - -0.6 4,843,302 - +0.4 4,843,364 + +0.7 4,843,364 + +0.3 4,843,371 - -0.9 4,843,389 + -1.2 4,843,410 + CA265_RS19535 0.13 +0.1 4,843,424 + CA265_RS19535 0.15 -1.2 4,843,425 - CA265_RS19535 0.15 +0.5 4,843,429 - CA265_RS19535 0.16 +1.1 4,843,429 - CA265_RS19535 0.16 +0.5 4,843,527 - CA265_RS19535 0.30 +1.6 4,843,552 - CA265_RS19535 0.33 +0.3 4,843,700 + CA265_RS19535 0.55 +0.6 4,843,711 + CA265_RS19535 0.57 +0.6 4,843,711 + CA265_RS19535 0.57 +0.7 4,843,714 - CA265_RS19535 0.57 -0.3 4,843,714 - CA265_RS19535 0.57 +1.0 4,843,744 - CA265_RS19535 0.61 +0.4 4,843,750 + CA265_RS19535 0.62 +0.3 4,843,786 - CA265_RS19535 0.67 +0.5 4,843,802 + CA265_RS19535 0.70 +0.1 4,843,835 + CA265_RS19535 0.74 +0.8 4,843,936 + CA265_RS19535 0.89 +0.4 4,843,936 + CA265_RS19535 0.89 +0.8 4,843,937 - CA265_RS19535 0.89 -0.1 4,843,937 - CA265_RS19535 0.89 +1.0 4,844,042 - +0.8 4,844,056 - +0.1 4,844,056 - +0.1 4,844,058 - -0.4 4,844,131 + +0.8 4,844,131 + -3.3 4,844,143 - +0.9 4,844,195 - +0.1 4,844,195 - +2.2 4,844,235 + -1.3 4,844,245 - +0.2 4,844,246 + -0.5 4,844,246 + -1.0 4,844,371 - +0.2 4,844,440 - +0.5 4,844,490 + CA265_RS19540 0.11 +0.3 4,844,494 + CA265_RS19540 0.11 +0.9 4,844,495 - CA265_RS19540 0.11 +1.0 4,844,497 - CA265_RS19540 0.11 +1.0 4,844,566 + CA265_RS19540 0.14 -0.1 4,844,566 + CA265_RS19540 0.14 +0.3 4,844,566 + CA265_RS19540 0.14 -0.2 4,844,567 - CA265_RS19540 0.14 -0.2 4,844,568 + CA265_RS19540 0.14 +0.9 4,844,568 + CA265_RS19540 0.14 +1.5 4,844,569 - CA265_RS19540 0.14 +0.8 4,844,574 - CA265_RS19540 0.14 +0.5 4,844,574 - CA265_RS19540 0.14 +1.0 4,844,601 - CA265_RS19540 0.16 +1.2 4,844,601 - CA265_RS19540 0.16 +0.8 4,844,622 + CA265_RS19540 0.17 -0.6 4,844,630 - CA265_RS19540 0.17 +0.8 4,844,660 - CA265_RS19540 0.18 +0.9 4,844,660 - CA265_RS19540 0.18 -0.7 4,844,710 + CA265_RS19540 0.21 -0.6 4,844,710 + CA265_RS19540 0.21 -1.1 4,844,710 + CA265_RS19540 0.21 +0.9 4,844,785 - CA265_RS19540 0.24 +0.5 4,844,789 - CA265_RS19540 0.24 -0.2 4,844,850 + CA265_RS19540 0.27 +0.3 4,844,949 + CA265_RS19540 0.32 +1.2 4,844,950 - CA265_RS19540 0.32 +0.5 4,844,950 - CA265_RS19540 0.32 -1.4 4,844,994 + CA265_RS19540 0.34 +0.4 4,844,994 + CA265_RS19540 0.34 +0.6 4,844,995 - CA265_RS19540 0.34 -0.4 4,845,035 + CA265_RS19540 0.36 -0.2 4,845,036 - CA265_RS19540 0.36 +0.2 4,845,088 - CA265_RS19540 0.38 -0.4 4,845,101 + CA265_RS19540 0.39 -0.9 4,845,102 - CA265_RS19540 0.39 +0.5 4,845,102 - CA265_RS19540 0.39 +1.1 4,845,120 + CA265_RS19540 0.39 +0.3 4,845,120 + CA265_RS19540 0.39 -0.1 4,845,152 + CA265_RS19540 0.41 -0.2 4,845,153 - CA265_RS19540 0.41 +0.5 4,845,185 + CA265_RS19540 0.42 +0.6 4,845,185 + CA265_RS19540 0.42 +0.4 4,845,186 - CA265_RS19540 0.42 -0.0 4,845,278 + CA265_RS19540 0.47 +2.5 4,845,279 - CA265_RS19540 0.47 +0.4 4,845,348 + CA265_RS19540 0.50 -1.0 4,845,369 + CA265_RS19540 0.51 +0.4 4,845,388 + CA265_RS19540 0.52 +1.0 4,845,388 + CA265_RS19540 0.52 -1.4 4,845,389 - CA265_RS19540 0.52 +0.6 4,845,389 - CA265_RS19540 0.52 +0.7 4,845,488 + CA265_RS19540 0.56 +1.2 4,845,501 - CA265_RS19540 0.57 +0.3 4,845,571 - CA265_RS19540 0.60 +0.6 4,845,642 - CA265_RS19540 0.63 -0.1 4,845,671 + CA265_RS19540 0.65 -0.1 4,845,717 - CA265_RS19540 0.67 +1.1 4,845,735 + CA265_RS19540 0.67 +1.4 4,845,738 + CA265_RS19540 0.68 +0.3 4,845,738 + CA265_RS19540 0.68 -1.9 4,845,763 - CA265_RS19540 0.69 -0.6 4,845,836 + CA265_RS19540 0.72 -2.2 4,845,844 - CA265_RS19540 0.72 -0.1 4,845,861 + +0.9 4,845,862 - CA265_RS19540 0.73 -0.2 4,846,009 - CA265_RS19540 0.80 +0.5 4,846,214 + CA265_RS19540 0.89 +0.9 4,846,408 + -0.6 4,846,409 - -0.2 4,846,409 - -0.6 4,846,444 + +0.2 4,846,455 - +0.6 4,846,632 + +0.2 4,846,632 + -0.0 4,846,705 + +0.2 4,846,828 + CA265_RS19545 0.17 -0.4 4,846,878 + CA265_RS19545 0.22 +1.0 4,846,977 + CA265_RS19545 0.33 +0.2 4,846,978 - CA265_RS19545 0.33 +0.6 4,847,010 + CA265_RS19545 0.36 +0.6 4,847,086 - CA265_RS19545 0.44 -1.4 4,847,103 + CA265_RS19545 0.46 +0.0 4,847,103 + CA265_RS19545 0.46 +1.3 4,847,106 + CA265_RS19545 0.46 +0.7 4,847,234 + CA265_RS19545 0.60 -0.5 4,847,234 + CA265_RS19545 0.60 -0.3 4,847,234 + CA265_RS19545 0.60 -0.5 4,847,235 - CA265_RS19545 0.60 +0.5 4,847,278 + CA265_RS19545 0.65 +1.6 4,847,293 + CA265_RS19545 0.66 -1.6 4,847,293 + CA265_RS19545 0.66 +0.3 4,847,301 + CA265_RS19545 0.67 +1.6 4,847,348 + CA265_RS19545 0.72 -0.5 4,847,349 - CA265_RS19545 0.72 +1.2 4,847,354 - CA265_RS19545 0.73 -1.9 4,847,369 + CA265_RS19545 0.74 -0.8
Or see this region's nucleotide sequence