Experiment: R2A with Nalidixic 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS18680 and CA265_RS18685 are separated by 90 nucleotides CA265_RS18685 and CA265_RS18690 are separated by 91 nucleotides
CA265_RS18680: CA265_RS18680 - hypothetical protein, at 4,643,908 to 4,644,786
_RS18680
CA265_RS18685: CA265_RS18685 - epimerase, at 4,644,877 to 4,645,548
_RS18685
CA265_RS18690: CA265_RS18690 - S9 family peptidase, at 4,645,640 to 4,647,775
_RS18690
Position (kb)
4644
4645
4646 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4643.950 kb on - strand at 4644.145 kb on + strand at 4644.145 kb on + strand, within CA265_RS18680 at 4644.146 kb on - strand, within CA265_RS18680 at 4644.146 kb on - strand, within CA265_RS18680 at 4644.202 kb on - strand, within CA265_RS18680 at 4644.206 kb on + strand, within CA265_RS18680 at 4644.277 kb on + strand, within CA265_RS18680 at 4644.277 kb on + strand, within CA265_RS18680 at 4644.277 kb on + strand, within CA265_RS18680 at 4644.296 kb on + strand, within CA265_RS18680 at 4644.296 kb on + strand, within CA265_RS18680 at 4644.297 kb on - strand, within CA265_RS18680 at 4644.342 kb on + strand, within CA265_RS18680 at 4644.343 kb on - strand, within CA265_RS18680 at 4644.352 kb on - strand, within CA265_RS18680 at 4644.370 kb on - strand, within CA265_RS18680 at 4644.425 kb on + strand, within CA265_RS18680 at 4644.444 kb on + strand, within CA265_RS18680 at 4644.489 kb on + strand, within CA265_RS18680 at 4644.489 kb on + strand, within CA265_RS18680 at 4644.490 kb on - strand, within CA265_RS18680 at 4644.547 kb on - strand, within CA265_RS18680 at 4644.547 kb on - strand, within CA265_RS18680 at 4644.805 kb on - strand at 4644.805 kb on - strand at 4644.814 kb on + strand at 4644.824 kb on + strand at 4644.866 kb on - strand at 4644.866 kb on - strand at 4644.996 kb on - strand, within CA265_RS18685 at 4645.029 kb on + strand, within CA265_RS18685 at 4645.031 kb on + strand, within CA265_RS18685 at 4645.053 kb on - strand, within CA265_RS18685 at 4645.075 kb on - strand, within CA265_RS18685 at 4645.095 kb on + strand, within CA265_RS18685 at 4645.261 kb on - strand, within CA265_RS18685 at 4645.278 kb on - strand, within CA265_RS18685 at 4645.281 kb on + strand, within CA265_RS18685 at 4645.297 kb on + strand, within CA265_RS18685 at 4645.332 kb on + strand, within CA265_RS18685 at 4645.386 kb on + strand, within CA265_RS18685 at 4645.401 kb on + strand, within CA265_RS18685 at 4645.410 kb on + strand, within CA265_RS18685 at 4645.480 kb on + strand, within CA265_RS18685 at 4645.520 kb on - strand at 4645.553 kb on - strand at 4645.601 kb on - strand at 4645.622 kb on - strand at 4645.675 kb on + strand at 4645.703 kb on + strand at 4645.703 kb on + strand at 4645.704 kb on - strand at 4645.704 kb on - strand at 4645.732 kb on + strand at 4645.744 kb on - strand at 4645.808 kb on + strand at 4645.809 kb on - strand at 4645.826 kb on + strand at 4645.827 kb on - strand at 4645.844 kb on + strand at 4645.849 kb on + strand at 4645.868 kb on + strand, within CA265_RS18690 at 4645.902 kb on + strand, within CA265_RS18690 at 4645.926 kb on + strand, within CA265_RS18690 at 4646.102 kb on - strand, within CA265_RS18690 at 4646.170 kb on + strand, within CA265_RS18690 at 4646.229 kb on - strand, within CA265_RS18690 at 4646.231 kb on + strand, within CA265_RS18690 at 4646.242 kb on + strand, within CA265_RS18690 at 4646.303 kb on + strand, within CA265_RS18690 at 4646.333 kb on - strand, within CA265_RS18690 at 4646.334 kb on + strand, within CA265_RS18690 at 4646.334 kb on + strand, within CA265_RS18690 at 4646.492 kb on - strand, within CA265_RS18690 at 4646.512 kb on + strand, within CA265_RS18690 at 4646.512 kb on + strand, within CA265_RS18690 at 4646.513 kb on - strand, within CA265_RS18690
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nalidixic 0.008 mg/ml remove 4,643,950 - -0.8 4,644,145 + +0.5 4,644,145 + CA265_RS18680 0.27 -0.7 4,644,146 - CA265_RS18680 0.27 -0.9 4,644,146 - CA265_RS18680 0.27 -1.9 4,644,202 - CA265_RS18680 0.33 -0.8 4,644,206 + CA265_RS18680 0.34 +0.4 4,644,277 + CA265_RS18680 0.42 -1.1 4,644,277 + CA265_RS18680 0.42 -0.2 4,644,277 + CA265_RS18680 0.42 +0.1 4,644,296 + CA265_RS18680 0.44 +1.1 4,644,296 + CA265_RS18680 0.44 -1.3 4,644,297 - CA265_RS18680 0.44 -0.3 4,644,342 + CA265_RS18680 0.49 +0.1 4,644,343 - CA265_RS18680 0.49 -0.9 4,644,352 - CA265_RS18680 0.51 +0.4 4,644,370 - CA265_RS18680 0.53 -1.0 4,644,425 + CA265_RS18680 0.59 -0.9 4,644,444 + CA265_RS18680 0.61 -1.1 4,644,489 + CA265_RS18680 0.66 -0.3 4,644,489 + CA265_RS18680 0.66 -0.6 4,644,490 - CA265_RS18680 0.66 -0.5 4,644,547 - CA265_RS18680 0.73 -0.9 4,644,547 - CA265_RS18680 0.73 -0.3 4,644,805 - +0.4 4,644,805 - +0.1 4,644,814 + -0.7 4,644,824 + -2.5 4,644,866 - -0.9 4,644,866 - +0.3 4,644,996 - CA265_RS18685 0.18 -1.5 4,645,029 + CA265_RS18685 0.23 +0.8 4,645,031 + CA265_RS18685 0.23 +0.6 4,645,053 - CA265_RS18685 0.26 -0.4 4,645,075 - CA265_RS18685 0.29 -1.2 4,645,095 + CA265_RS18685 0.32 +1.4 4,645,261 - CA265_RS18685 0.57 -1.2 4,645,278 - CA265_RS18685 0.60 -1.6 4,645,281 + CA265_RS18685 0.60 +0.1 4,645,297 + CA265_RS18685 0.62 -1.0 4,645,332 + CA265_RS18685 0.68 -1.7 4,645,386 + CA265_RS18685 0.76 -0.8 4,645,401 + CA265_RS18685 0.78 -2.8 4,645,410 + CA265_RS18685 0.79 +0.0 4,645,480 + CA265_RS18685 0.90 -1.0 4,645,520 - -2.5 4,645,553 - +0.2 4,645,601 - -0.2 4,645,622 - -0.7 4,645,675 + +0.1 4,645,703 + +0.1 4,645,703 + -0.7 4,645,704 - -0.6 4,645,704 - +0.0 4,645,732 + -0.0 4,645,744 - +0.3 4,645,808 + +1.6 4,645,809 - -0.1 4,645,826 + +1.1 4,645,827 - -1.0 4,645,844 + -0.2 4,645,849 + +0.3 4,645,868 + CA265_RS18690 0.11 +1.1 4,645,902 + CA265_RS18690 0.12 -1.2 4,645,926 + CA265_RS18690 0.13 -0.1 4,646,102 - CA265_RS18690 0.22 +0.1 4,646,170 + CA265_RS18690 0.25 -1.3 4,646,229 - CA265_RS18690 0.28 -0.1 4,646,231 + CA265_RS18690 0.28 -0.3 4,646,242 + CA265_RS18690 0.28 +0.4 4,646,303 + CA265_RS18690 0.31 +0.6 4,646,333 - CA265_RS18690 0.32 -2.2 4,646,334 + CA265_RS18690 0.32 +0.4 4,646,334 + CA265_RS18690 0.32 +1.1 4,646,492 - CA265_RS18690 0.40 -0.2 4,646,512 + CA265_RS18690 0.41 +0.5 4,646,512 + CA265_RS18690 0.41 -0.9 4,646,513 - CA265_RS18690 0.41 -1.1
Or see this region's nucleotide sequence