Experiment: R2A with Nalidixic 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS18260 and CA265_RS18265 are separated by 161 nucleotides CA265_RS18265 and CA265_RS18270 are separated by 108 nucleotides CA265_RS18270 and CA265_RS18275 are separated by 44 nucleotides CA265_RS18275 and CA265_RS18280 overlap by 11 nucleotides
CA265_RS18260: CA265_RS18260 - hypothetical protein, at 4,552,545 to 4,552,934
_RS18260
CA265_RS18265: CA265_RS18265 - hypothetical protein, at 4,553,096 to 4,553,359
_RS18265
CA265_RS18270: CA265_RS18270 - hypothetical protein, at 4,553,468 to 4,553,779
_RS18270
CA265_RS18275: CA265_RS18275 - pyrophosphatase, at 4,553,824 to 4,554,153
_RS18275
CA265_RS18280: CA265_RS18280 - hypothetical protein, at 4,554,143 to 4,555,705
_RS18280
Position (kb)
4553
4554
4555 Strain fitness (log2 ratio)
-1
0
1
2 at 4552.898 kb on + strand at 4552.934 kb on + strand at 4552.935 kb on - strand at 4552.935 kb on - strand at 4552.935 kb on - strand at 4552.937 kb on - strand at 4553.002 kb on - strand at 4553.002 kb on - strand at 4553.025 kb on + strand at 4553.026 kb on - strand at 4553.026 kb on - strand at 4553.026 kb on - strand at 4553.053 kb on - strand at 4553.053 kb on - strand at 4553.058 kb on + strand at 4553.058 kb on + strand at 4553.117 kb on - strand at 4553.129 kb on + strand, within CA265_RS18265 at 4553.218 kb on + strand, within CA265_RS18265 at 4553.302 kb on + strand, within CA265_RS18265 at 4553.304 kb on + strand, within CA265_RS18265 at 4553.305 kb on - strand, within CA265_RS18265 at 4553.311 kb on - strand, within CA265_RS18265 at 4553.311 kb on - strand, within CA265_RS18265 at 4553.355 kb on - strand at 4553.445 kb on + strand at 4553.447 kb on + strand at 4553.449 kb on + strand at 4553.449 kb on + strand at 4553.456 kb on + strand at 4553.480 kb on - strand at 4553.496 kb on + strand at 4553.581 kb on + strand, within CA265_RS18270 at 4553.627 kb on + strand, within CA265_RS18270 at 4553.639 kb on + strand, within CA265_RS18270 at 4553.640 kb on - strand, within CA265_RS18270 at 4553.640 kb on - strand, within CA265_RS18270 at 4553.668 kb on + strand, within CA265_RS18270 at 4553.802 kb on + strand at 4553.953 kb on + strand, within CA265_RS18275 at 4553.953 kb on + strand, within CA265_RS18275 at 4553.954 kb on - strand, within CA265_RS18275 at 4553.954 kb on - strand, within CA265_RS18275 at 4553.954 kb on - strand, within CA265_RS18275 at 4553.954 kb on - strand, within CA265_RS18275 at 4554.017 kb on + strand, within CA265_RS18275 at 4554.021 kb on + strand at 4554.105 kb on + strand, within CA265_RS18275 at 4554.167 kb on + strand at 4554.167 kb on + strand at 4554.194 kb on - strand at 4554.396 kb on + strand, within CA265_RS18280 at 4554.402 kb on - strand, within CA265_RS18280 at 4554.456 kb on + strand, within CA265_RS18280 at 4554.510 kb on - strand, within CA265_RS18280 at 4554.601 kb on + strand, within CA265_RS18280 at 4554.601 kb on + strand, within CA265_RS18280 at 4554.601 kb on + strand, within CA265_RS18280 at 4554.606 kb on - strand, within CA265_RS18280 at 4554.610 kb on + strand at 4554.632 kb on + strand, within CA265_RS18280 at 4554.651 kb on - strand, within CA265_RS18280 at 4554.683 kb on - strand, within CA265_RS18280 at 4554.713 kb on + strand, within CA265_RS18280 at 4554.804 kb on + strand, within CA265_RS18280 at 4554.849 kb on - strand, within CA265_RS18280 at 4554.850 kb on + strand, within CA265_RS18280 at 4554.889 kb on + strand, within CA265_RS18280 at 4554.926 kb on - strand, within CA265_RS18280 at 4554.937 kb on + strand, within CA265_RS18280 at 4555.015 kb on + strand, within CA265_RS18280 at 4555.016 kb on - strand, within CA265_RS18280 at 4555.044 kb on + strand, within CA265_RS18280
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nalidixic 0.008 mg/ml remove 4,552,898 + -0.7 4,552,934 + +0.3 4,552,935 - -0.2 4,552,935 - +0.4 4,552,935 - -0.6 4,552,937 - -1.3 4,553,002 - -0.7 4,553,002 - -1.7 4,553,025 + +0.6 4,553,026 - -1.1 4,553,026 - +2.0 4,553,026 - -0.5 4,553,053 - -0.1 4,553,053 - -1.3 4,553,058 + +0.1 4,553,058 + +0.7 4,553,117 - -0.1 4,553,129 + CA265_RS18265 0.12 +0.2 4,553,218 + CA265_RS18265 0.46 -0.8 4,553,302 + CA265_RS18265 0.78 +0.2 4,553,304 + CA265_RS18265 0.79 +0.0 4,553,305 - CA265_RS18265 0.79 -0.6 4,553,311 - CA265_RS18265 0.81 +0.7 4,553,311 - CA265_RS18265 0.81 -0.1 4,553,355 - -0.6 4,553,445 + -0.2 4,553,447 + -0.0 4,553,449 + +0.2 4,553,449 + +0.2 4,553,456 + +0.6 4,553,480 - -0.2 4,553,496 + +0.2 4,553,581 + CA265_RS18270 0.36 -0.3 4,553,627 + CA265_RS18270 0.51 -1.0 4,553,639 + CA265_RS18270 0.55 +0.0 4,553,640 - CA265_RS18270 0.55 -0.3 4,553,640 - CA265_RS18270 0.55 -0.9 4,553,668 + CA265_RS18270 0.64 +1.0 4,553,802 + -0.1 4,553,953 + CA265_RS18275 0.39 +2.0 4,553,953 + CA265_RS18275 0.39 +1.6 4,553,954 - CA265_RS18275 0.39 +0.9 4,553,954 - CA265_RS18275 0.39 -0.5 4,553,954 - CA265_RS18275 0.39 +1.4 4,553,954 - CA265_RS18275 0.39 +0.2 4,554,017 + CA265_RS18275 0.58 +0.8 4,554,021 + +2.0 4,554,105 + CA265_RS18275 0.85 -1.2 4,554,167 + -0.0 4,554,167 + +0.9 4,554,194 - -0.4 4,554,396 + CA265_RS18280 0.16 +0.5 4,554,402 - CA265_RS18280 0.17 -0.3 4,554,456 + CA265_RS18280 0.20 +0.1 4,554,510 - CA265_RS18280 0.23 +0.1 4,554,601 + CA265_RS18280 0.29 +0.0 4,554,601 + CA265_RS18280 0.29 +0.4 4,554,601 + CA265_RS18280 0.29 -0.0 4,554,606 - CA265_RS18280 0.30 +0.7 4,554,610 + +2.0 4,554,632 + CA265_RS18280 0.31 +0.3 4,554,651 - CA265_RS18280 0.33 -0.5 4,554,683 - CA265_RS18280 0.35 -0.0 4,554,713 + CA265_RS18280 0.36 +0.8 4,554,804 + CA265_RS18280 0.42 -0.5 4,554,849 - CA265_RS18280 0.45 +0.5 4,554,850 + CA265_RS18280 0.45 +1.3 4,554,889 + CA265_RS18280 0.48 +0.8 4,554,926 - CA265_RS18280 0.50 +0.1 4,554,937 + CA265_RS18280 0.51 +0.1 4,555,015 + CA265_RS18280 0.56 -0.3 4,555,016 - CA265_RS18280 0.56 -0.0 4,555,044 + CA265_RS18280 0.58 +0.4
Or see this region's nucleotide sequence