Experiment: R2A with Nalidixic 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS01590 and CA265_RS01595 are separated by 9 nucleotides CA265_RS01595 and CA265_RS01600 overlap by 1 nucleotides
CA265_RS01590: CA265_RS01590 - sulfatase, at 410,577 to 412,028
_RS01590
CA265_RS01595: CA265_RS01595 - 6-bladed beta-propeller, at 412,038 to 413,063
_RS01595
CA265_RS01600: CA265_RS01600 - cytochrome C, at 413,063 to 415,225
_RS01600
Position (kb)
412
413
414 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 411.092 kb on + strand, within CA265_RS01590 at 411.101 kb on + strand, within CA265_RS01590 at 411.186 kb on - strand, within CA265_RS01590 at 411.199 kb on + strand, within CA265_RS01590 at 411.200 kb on - strand, within CA265_RS01590 at 411.218 kb on + strand, within CA265_RS01590 at 411.250 kb on + strand, within CA265_RS01590 at 411.282 kb on + strand, within CA265_RS01590 at 411.409 kb on - strand, within CA265_RS01590 at 411.475 kb on + strand, within CA265_RS01590 at 411.476 kb on + strand, within CA265_RS01590 at 411.476 kb on + strand, within CA265_RS01590 at 411.476 kb on + strand, within CA265_RS01590 at 411.489 kb on + strand, within CA265_RS01590 at 411.551 kb on - strand, within CA265_RS01590 at 411.646 kb on + strand, within CA265_RS01590 at 411.679 kb on + strand, within CA265_RS01590 at 411.775 kb on - strand, within CA265_RS01590 at 411.804 kb on - strand, within CA265_RS01590 at 411.804 kb on - strand, within CA265_RS01590 at 411.809 kb on - strand, within CA265_RS01590 at 411.893 kb on - strand at 412.022 kb on - strand at 412.027 kb on - strand at 412.056 kb on - strand at 412.067 kb on + strand at 412.132 kb on - strand at 412.140 kb on - strand at 412.142 kb on + strand, within CA265_RS01595 at 412.153 kb on - strand, within CA265_RS01595 at 412.232 kb on + strand, within CA265_RS01595 at 412.233 kb on - strand, within CA265_RS01595 at 412.245 kb on - strand, within CA265_RS01595 at 412.289 kb on + strand, within CA265_RS01595 at 412.347 kb on - strand, within CA265_RS01595 at 412.474 kb on - strand, within CA265_RS01595 at 412.474 kb on - strand, within CA265_RS01595 at 412.513 kb on + strand, within CA265_RS01595 at 412.514 kb on - strand, within CA265_RS01595 at 412.560 kb on + strand, within CA265_RS01595 at 412.565 kb on - strand, within CA265_RS01595 at 412.600 kb on - strand, within CA265_RS01595 at 412.801 kb on - strand, within CA265_RS01595 at 412.807 kb on - strand, within CA265_RS01595 at 412.807 kb on - strand, within CA265_RS01595 at 412.917 kb on + strand, within CA265_RS01595 at 412.918 kb on - strand, within CA265_RS01595 at 412.930 kb on + strand, within CA265_RS01595 at 412.931 kb on - strand, within CA265_RS01595 at 412.960 kb on + strand, within CA265_RS01595 at 413.008 kb on - strand at 413.060 kb on - strand at 413.061 kb on + strand at 413.062 kb on - strand at 413.119 kb on + strand at 413.194 kb on + strand at 413.199 kb on + strand at 413.263 kb on + strand at 413.296 kb on + strand, within CA265_RS01600 at 413.366 kb on - strand, within CA265_RS01600 at 413.419 kb on + strand, within CA265_RS01600 at 413.420 kb on - strand, within CA265_RS01600 at 413.420 kb on - strand, within CA265_RS01600 at 413.443 kb on + strand, within CA265_RS01600 at 413.447 kb on + strand, within CA265_RS01600 at 413.506 kb on + strand, within CA265_RS01600 at 413.507 kb on - strand, within CA265_RS01600 at 413.631 kb on + strand, within CA265_RS01600 at 413.699 kb on - strand, within CA265_RS01600 at 413.737 kb on + strand, within CA265_RS01600 at 413.796 kb on + strand, within CA265_RS01600 at 413.805 kb on + strand, within CA265_RS01600 at 413.805 kb on + strand, within CA265_RS01600 at 413.833 kb on + strand, within CA265_RS01600 at 413.952 kb on + strand, within CA265_RS01600 at 414.038 kb on - strand, within CA265_RS01600 at 414.052 kb on + strand, within CA265_RS01600 at 414.053 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nalidixic 0.008 mg/ml remove 411,092 + CA265_RS01590 0.35 +0.3 411,101 + CA265_RS01590 0.36 -0.8 411,186 - CA265_RS01590 0.42 +0.1 411,199 + CA265_RS01590 0.43 -1.1 411,200 - CA265_RS01590 0.43 -0.9 411,218 + CA265_RS01590 0.44 -2.9 411,250 + CA265_RS01590 0.46 +0.7 411,282 + CA265_RS01590 0.49 +0.6 411,409 - CA265_RS01590 0.57 +0.0 411,475 + CA265_RS01590 0.62 +0.7 411,476 + CA265_RS01590 0.62 -0.3 411,476 + CA265_RS01590 0.62 -0.4 411,476 + CA265_RS01590 0.62 -0.0 411,489 + CA265_RS01590 0.63 -0.6 411,551 - CA265_RS01590 0.67 +0.3 411,646 + CA265_RS01590 0.74 +0.4 411,679 + CA265_RS01590 0.76 -0.3 411,775 - CA265_RS01590 0.83 -1.2 411,804 - CA265_RS01590 0.85 +0.9 411,804 - CA265_RS01590 0.85 -0.5 411,809 - CA265_RS01590 0.85 -0.5 411,893 - -0.0 412,022 - +0.2 412,027 - -0.4 412,056 - +0.9 412,067 + +0.5 412,132 - +0.3 412,140 - -0.2 412,142 + CA265_RS01595 0.10 -1.3 412,153 - CA265_RS01595 0.11 -0.8 412,232 + CA265_RS01595 0.19 -1.3 412,233 - CA265_RS01595 0.19 -2.3 412,245 - CA265_RS01595 0.20 +0.2 412,289 + CA265_RS01595 0.24 +0.4 412,347 - CA265_RS01595 0.30 +0.1 412,474 - CA265_RS01595 0.42 +0.3 412,474 - CA265_RS01595 0.42 +2.1 412,513 + CA265_RS01595 0.46 -0.1 412,514 - CA265_RS01595 0.46 +0.1 412,560 + CA265_RS01595 0.51 -0.8 412,565 - CA265_RS01595 0.51 +0.6 412,600 - CA265_RS01595 0.55 +0.9 412,801 - CA265_RS01595 0.74 -2.5 412,807 - CA265_RS01595 0.75 -1.7 412,807 - CA265_RS01595 0.75 -0.4 412,917 + CA265_RS01595 0.86 -0.6 412,918 - CA265_RS01595 0.86 -0.4 412,930 + CA265_RS01595 0.87 -0.4 412,931 - CA265_RS01595 0.87 +0.1 412,960 + CA265_RS01595 0.90 -0.2 413,008 - -0.5 413,060 - +0.4 413,061 + -0.4 413,062 - +0.5 413,119 + +0.6 413,194 + -0.9 413,199 + +0.1 413,263 + +0.1 413,296 + CA265_RS01600 0.11 -0.8 413,366 - CA265_RS01600 0.14 -0.9 413,419 + CA265_RS01600 0.16 +1.2 413,420 - CA265_RS01600 0.17 -0.5 413,420 - CA265_RS01600 0.17 +0.6 413,443 + CA265_RS01600 0.18 -0.1 413,447 + CA265_RS01600 0.18 -0.5 413,506 + CA265_RS01600 0.20 +0.5 413,507 - CA265_RS01600 0.21 +0.6 413,631 + CA265_RS01600 0.26 -0.1 413,699 - CA265_RS01600 0.29 +0.5 413,737 + CA265_RS01600 0.31 -0.5 413,796 + CA265_RS01600 0.34 +0.5 413,805 + CA265_RS01600 0.34 -0.3 413,805 + CA265_RS01600 0.34 +0.4 413,833 + CA265_RS01600 0.36 -0.4 413,952 + CA265_RS01600 0.41 -0.2 414,038 - CA265_RS01600 0.45 +0.2 414,052 + CA265_RS01600 0.46 +0.1 414,053 - +0.6
Or see this region's nucleotide sequence