Experiment: R2A with Nalidixic 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS00220 and CA265_RS00225 are separated by 84 nucleotides CA265_RS00225 and CA265_RS00230 are separated by 160 nucleotides CA265_RS00230 and CA265_RS00235 are separated by 150 nucleotides CA265_RS00235 and CA265_RS00240 are separated by 5 nucleotides
CA265_RS00220: CA265_RS00220 - potassium-transporting ATPase subunit B, at 53,565 to 55,592
_RS00220
CA265_RS00225: CA265_RS00225 - potassium-transporting ATPase subunit C, at 55,677 to 56,237
_RS00225
CA265_RS00230: CA265_RS00230 - hypothetical protein, at 56,398 to 57,468
_RS00230
CA265_RS00235: CA265_RS00235 - hypothetical protein, at 57,619 to 57,867
_RS00235
CA265_RS00240: CA265_RS00240 - histidine kinase, at 57,873 to 59,003
_RS00240
Position (kb) 56
57
58 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 55.430 kb on + strand at 55.470 kb on + strand at 55.495 kb on + strand at 55.516 kb on + strand at 55.516 kb on + strand at 55.516 kb on + strand at 55.517 kb on - strand at 55.517 kb on - strand at 55.517 kb on - strand at 55.517 kb on - strand at 55.517 kb on - strand at 55.517 kb on - strand at 55.519 kb on - strand at 55.519 kb on - strand at 55.519 kb on - strand at 55.547 kb on + strand at 55.547 kb on + strand at 55.549 kb on + strand at 55.718 kb on - strand at 55.732 kb on + strand at 55.738 kb on + strand, within CA265_RS00225 at 55.739 kb on - strand, within CA265_RS00225 at 55.739 kb on - strand, within CA265_RS00225 at 55.739 kb on - strand, within CA265_RS00225 at 55.741 kb on + strand, within CA265_RS00225 at 55.742 kb on - strand, within CA265_RS00225 at 55.742 kb on - strand, within CA265_RS00225 at 55.769 kb on + strand, within CA265_RS00225 at 55.867 kb on - strand, within CA265_RS00225 at 55.896 kb on + strand, within CA265_RS00225 at 55.896 kb on + strand, within CA265_RS00225 at 55.897 kb on - strand, within CA265_RS00225 at 55.952 kb on - strand, within CA265_RS00225 at 55.990 kb on - strand, within CA265_RS00225 at 56.015 kb on + strand, within CA265_RS00225 at 56.016 kb on - strand, within CA265_RS00225 at 56.064 kb on - strand, within CA265_RS00225 at 56.144 kb on - strand, within CA265_RS00225 at 56.197 kb on + strand at 56.235 kb on + strand at 56.300 kb on - strand at 56.414 kb on + strand at 56.598 kb on + strand, within CA265_RS00230 at 56.609 kb on - strand, within CA265_RS00230 at 56.691 kb on - strand, within CA265_RS00230 at 56.829 kb on + strand, within CA265_RS00230 at 56.851 kb on + strand, within CA265_RS00230 at 56.854 kb on + strand, within CA265_RS00230 at 56.878 kb on + strand, within CA265_RS00230 at 56.879 kb on - strand, within CA265_RS00230 at 56.887 kb on - strand, within CA265_RS00230 at 56.917 kb on + strand, within CA265_RS00230 at 56.918 kb on - strand, within CA265_RS00230 at 56.962 kb on - strand, within CA265_RS00230 at 57.003 kb on + strand, within CA265_RS00230 at 57.003 kb on + strand, within CA265_RS00230 at 57.085 kb on + strand, within CA265_RS00230 at 57.085 kb on + strand, within CA265_RS00230 at 57.091 kb on - strand, within CA265_RS00230 at 57.171 kb on + strand, within CA265_RS00230 at 57.210 kb on + strand, within CA265_RS00230 at 57.253 kb on + strand, within CA265_RS00230 at 57.254 kb on - strand, within CA265_RS00230 at 57.316 kb on + strand, within CA265_RS00230 at 57.334 kb on + strand, within CA265_RS00230 at 57.601 kb on - strand at 57.619 kb on + strand at 57.633 kb on - strand at 57.663 kb on - strand, within CA265_RS00235 at 57.663 kb on - strand, within CA265_RS00235 at 57.787 kb on - strand, within CA265_RS00235 at 57.831 kb on - strand, within CA265_RS00235 at 57.848 kb on + strand at 57.993 kb on - strand, within CA265_RS00240 at 58.126 kb on - strand, within CA265_RS00240 at 58.126 kb on - strand, within CA265_RS00240 at 58.146 kb on + strand, within CA265_RS00240 at 58.190 kb on + strand, within CA265_RS00240 at 58.212 kb on - strand, within CA265_RS00240 at 58.367 kb on + strand, within CA265_RS00240 at 58.381 kb on + strand, within CA265_RS00240 at 58.390 kb on + strand, within CA265_RS00240 at 58.390 kb on + strand, within CA265_RS00240
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nalidixic 0.008 mg/ml remove 55,430 + +0.3 55,470 + +0.7 55,495 + +1.0 55,516 + -2.5 55,516 + -1.0 55,516 + -3.3 55,517 - -1.3 55,517 - +0.0 55,517 - -0.0 55,517 - +2.1 55,517 - +0.3 55,517 - -0.1 55,519 - -0.7 55,519 - -1.1 55,519 - -1.3 55,547 + -1.4 55,547 + -2.5 55,549 + -1.4 55,718 - -0.1 55,732 + -2.4 55,738 + CA265_RS00225 0.11 -2.2 55,739 - CA265_RS00225 0.11 -0.2 55,739 - CA265_RS00225 0.11 +0.4 55,739 - CA265_RS00225 0.11 -0.7 55,741 + CA265_RS00225 0.11 -3.3 55,742 - CA265_RS00225 0.12 -0.1 55,742 - CA265_RS00225 0.12 +0.1 55,769 + CA265_RS00225 0.16 +0.1 55,867 - CA265_RS00225 0.34 -0.2 55,896 + CA265_RS00225 0.39 -1.5 55,896 + CA265_RS00225 0.39 -1.8 55,897 - CA265_RS00225 0.39 -0.1 55,952 - CA265_RS00225 0.49 -1.5 55,990 - CA265_RS00225 0.56 +1.1 56,015 + CA265_RS00225 0.60 -0.9 56,016 - CA265_RS00225 0.60 -0.3 56,064 - CA265_RS00225 0.69 -0.6 56,144 - CA265_RS00225 0.83 -0.1 56,197 + -2.9 56,235 + -2.3 56,300 - -0.6 56,414 + -2.3 56,598 + CA265_RS00230 0.19 -1.1 56,609 - CA265_RS00230 0.20 -1.1 56,691 - CA265_RS00230 0.27 +0.5 56,829 + CA265_RS00230 0.40 -1.9 56,851 + CA265_RS00230 0.42 -1.5 56,854 + CA265_RS00230 0.43 -2.4 56,878 + CA265_RS00230 0.45 -1.8 56,879 - CA265_RS00230 0.45 -0.4 56,887 - CA265_RS00230 0.46 +0.5 56,917 + CA265_RS00230 0.48 -2.5 56,918 - CA265_RS00230 0.49 +0.2 56,962 - CA265_RS00230 0.53 -0.1 57,003 + CA265_RS00230 0.56 -1.4 57,003 + CA265_RS00230 0.56 -2.6 57,085 + CA265_RS00230 0.64 -2.9 57,085 + CA265_RS00230 0.64 -0.3 57,091 - CA265_RS00230 0.65 +0.6 57,171 + CA265_RS00230 0.72 -3.1 57,210 + CA265_RS00230 0.76 -2.1 57,253 + CA265_RS00230 0.80 -2.4 57,254 - CA265_RS00230 0.80 +1.7 57,316 + CA265_RS00230 0.86 -2.2 57,334 + CA265_RS00230 0.87 -2.9 57,601 - -0.8 57,619 + -1.4 57,633 - -0.3 57,663 - CA265_RS00235 0.18 -1.7 57,663 - CA265_RS00235 0.18 -0.6 57,787 - CA265_RS00235 0.67 -0.0 57,831 - CA265_RS00235 0.85 -1.7 57,848 + -0.6 57,993 - CA265_RS00240 0.11 -2.4 58,126 - CA265_RS00240 0.22 -1.0 58,126 - CA265_RS00240 0.22 +0.7 58,146 + CA265_RS00240 0.24 +0.0 58,190 + CA265_RS00240 0.28 +0.4 58,212 - CA265_RS00240 0.30 +0.4 58,367 + CA265_RS00240 0.44 -0.0 58,381 + CA265_RS00240 0.45 +0.9 58,390 + CA265_RS00240 0.46 -0.1 58,390 + CA265_RS00240 0.46 +0.5
Or see this region's nucleotide sequence