Experiment: reconstituted MRS with Melibiose 29 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14285 and BBR_RS14290 are separated by 33 nucleotides BBR_RS14290 and BBR_RS14295 are separated by 169 nucleotides
BBR_RS14285: BBR_RS14285 - excinuclease ABC subunit UvrB, at 979,424 to 981,535
_RS14285
BBR_RS14290: BBR_RS14290 - TerC family protein, at 981,569 to 982,555
_RS14290
BBR_RS14295: BBR_RS14295 - pyruvate kinase, at 982,725 to 984,167
_RS14295
Position (kb) 981
982
983 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 980.575 kb on - strand, within BBR_RS14285 at 980.601 kb on - strand, within BBR_RS14285 at 980.606 kb on + strand, within BBR_RS14285 at 980.608 kb on - strand, within BBR_RS14285 at 980.609 kb on + strand, within BBR_RS14285 at 980.615 kb on - strand, within BBR_RS14285 at 980.615 kb on - strand, within BBR_RS14285 at 980.622 kb on - strand, within BBR_RS14285 at 980.625 kb on + strand, within BBR_RS14285 at 980.639 kb on + strand, within BBR_RS14285 at 980.719 kb on + strand, within BBR_RS14285 at 980.734 kb on - strand, within BBR_RS14285 at 980.752 kb on + strand, within BBR_RS14285 at 980.760 kb on - strand, within BBR_RS14285 at 980.786 kb on + strand, within BBR_RS14285 at 980.876 kb on - strand, within BBR_RS14285 at 980.900 kb on - strand, within BBR_RS14285 at 980.925 kb on + strand, within BBR_RS14285 at 980.964 kb on + strand, within BBR_RS14285 at 981.116 kb on - strand, within BBR_RS14285 at 981.129 kb on + strand, within BBR_RS14285 at 981.186 kb on + strand, within BBR_RS14285 at 981.194 kb on - strand, within BBR_RS14285 at 981.238 kb on + strand, within BBR_RS14285 at 981.256 kb on - strand, within BBR_RS14285 at 981.266 kb on - strand, within BBR_RS14285 at 981.306 kb on - strand, within BBR_RS14285 at 981.311 kb on + strand, within BBR_RS14285 at 981.322 kb on + strand, within BBR_RS14285 at 981.425 kb on - strand at 981.425 kb on - strand at 981.427 kb on + strand at 981.433 kb on - strand at 981.435 kb on + strand at 981.435 kb on - strand at 981.448 kb on + strand at 981.630 kb on - strand at 981.873 kb on + strand, within BBR_RS14290 at 981.941 kb on + strand, within BBR_RS14290 at 981.999 kb on - strand, within BBR_RS14290 at 981.999 kb on - strand, within BBR_RS14290 at 982.059 kb on + strand, within BBR_RS14290 at 982.069 kb on + strand, within BBR_RS14290 at 982.069 kb on + strand, within BBR_RS14290 at 982.138 kb on + strand, within BBR_RS14290 at 982.138 kb on + strand, within BBR_RS14290 at 982.148 kb on - strand, within BBR_RS14290 at 982.148 kb on - strand, within BBR_RS14290 at 982.294 kb on + strand, within BBR_RS14290 at 982.315 kb on + strand, within BBR_RS14290 at 982.315 kb on + strand, within BBR_RS14290 at 982.376 kb on - strand, within BBR_RS14290 at 982.404 kb on - strand, within BBR_RS14290 at 982.462 kb on + strand at 982.501 kb on - strand at 982.502 kb on + strand at 982.644 kb on + strand at 982.652 kb on + strand at 982.714 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Melibiose 29 mM remove 980,575 - BBR_RS14285 0.54 +0.7 980,601 - BBR_RS14285 0.56 -0.6 980,606 + BBR_RS14285 0.56 -0.2 980,608 - BBR_RS14285 0.56 -0.7 980,609 + BBR_RS14285 0.56 +0.2 980,615 - BBR_RS14285 0.56 +0.8 980,615 - BBR_RS14285 0.56 -0.4 980,622 - BBR_RS14285 0.57 +0.1 980,625 + BBR_RS14285 0.57 -0.5 980,639 + BBR_RS14285 0.58 +0.2 980,719 + BBR_RS14285 0.61 +0.3 980,734 - BBR_RS14285 0.62 +0.8 980,752 + BBR_RS14285 0.63 +0.4 980,760 - BBR_RS14285 0.63 +0.0 980,786 + BBR_RS14285 0.64 -0.5 980,876 - BBR_RS14285 0.69 -0.0 980,900 - BBR_RS14285 0.70 +0.6 980,925 + BBR_RS14285 0.71 +0.2 980,964 + BBR_RS14285 0.73 -0.2 981,116 - BBR_RS14285 0.80 -0.3 981,129 + BBR_RS14285 0.81 +0.1 981,186 + BBR_RS14285 0.83 +0.2 981,194 - BBR_RS14285 0.84 +0.4 981,238 + BBR_RS14285 0.86 +0.1 981,256 - BBR_RS14285 0.87 -0.2 981,266 - BBR_RS14285 0.87 +0.5 981,306 - BBR_RS14285 0.89 -1.1 981,311 + BBR_RS14285 0.89 +0.6 981,322 + BBR_RS14285 0.90 -0.8 981,425 - +1.0 981,425 - -0.2 981,427 + -0.2 981,433 - +0.5 981,435 + -0.7 981,435 - +0.6 981,448 + -0.8 981,630 - -1.4 981,873 + BBR_RS14290 0.31 -1.2 981,941 + BBR_RS14290 0.38 +0.4 981,999 - BBR_RS14290 0.44 -1.4 981,999 - BBR_RS14290 0.44 -1.1 982,059 + BBR_RS14290 0.50 -0.5 982,069 + BBR_RS14290 0.51 -0.4 982,069 + BBR_RS14290 0.51 -0.8 982,138 + BBR_RS14290 0.58 -3.3 982,138 + BBR_RS14290 0.58 -2.7 982,148 - BBR_RS14290 0.59 -0.7 982,148 - BBR_RS14290 0.59 -2.5 982,294 + BBR_RS14290 0.73 -1.3 982,315 + BBR_RS14290 0.76 -1.7 982,315 + BBR_RS14290 0.76 -0.6 982,376 - BBR_RS14290 0.82 -1.7 982,404 - BBR_RS14290 0.85 -2.0 982,462 + -2.2 982,501 - -2.6 982,502 + -1.1 982,644 + -0.2 982,652 + +0.0 982,714 + -2.2
Or see this region's nucleotide sequence