Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS04340 and EX31_RS04345 are separated by 283 nucleotides EX31_RS04345 and EX31_RS25140 are separated by 16 nucleotides EX31_RS25140 and EX31_RS04355 are separated by 165 nucleotides
EX31_RS04340: EX31_RS04340 - tyrosine-type recombinase/integrase, at 397,627 to 398,832
_RS04340
EX31_RS04345: EX31_RS04345 - hypothetical protein, at 399,116 to 399,430
_RS04345
EX31_RS25140: EX31_RS25140 - AlpA family transcriptional regulator, at 399,447 to 399,653
_RS25140
EX31_RS04355: EX31_RS04355 - hypothetical protein, at 399,819 to 400,628
_RS04355
Position (kb) 399
400 Strain fitness (log2 ratio) -2
-1
0
1
2 at 398.466 kb on + strand, within EX31_RS04340 at 398.558 kb on + strand, within EX31_RS04340 at 398.631 kb on + strand, within EX31_RS04340 at 398.649 kb on + strand, within EX31_RS04340 at 398.658 kb on + strand, within EX31_RS04340 at 398.684 kb on - strand, within EX31_RS04340 at 398.685 kb on + strand, within EX31_RS04340 at 398.691 kb on + strand, within EX31_RS04340 at 398.803 kb on + strand at 398.833 kb on - strand at 398.852 kb on - strand at 398.858 kb on - strand at 398.861 kb on + strand at 398.862 kb on - strand at 398.874 kb on + strand at 398.974 kb on + strand at 399.070 kb on - strand at 399.566 kb on + strand, within EX31_RS25140 at 399.578 kb on - strand, within EX31_RS25140 at 399.609 kb on + strand, within EX31_RS25140 at 399.699 kb on - strand at 399.704 kb on + strand at 399.705 kb on - strand at 399.709 kb on - strand at 399.726 kb on - strand at 399.726 kb on - strand at 399.802 kb on - strand at 399.802 kb on - strand at 399.802 kb on - strand at 399.813 kb on + strand at 399.813 kb on + strand at 399.814 kb on - strand at 399.891 kb on - strand at 399.909 kb on + strand, within EX31_RS04355 at 399.909 kb on + strand, within EX31_RS04355 at 399.987 kb on + strand, within EX31_RS04355 at 399.999 kb on + strand, within EX31_RS04355 at 400.090 kb on - strand, within EX31_RS04355 at 400.204 kb on - strand, within EX31_RS04355 at 400.271 kb on + strand, within EX31_RS04355 at 400.271 kb on + strand, within EX31_RS04355 at 400.385 kb on + strand, within EX31_RS04355 at 400.385 kb on + strand, within EX31_RS04355 at 400.386 kb on - strand, within EX31_RS04355 at 400.386 kb on - strand, within EX31_RS04355 at 400.503 kb on - strand, within EX31_RS04355 at 400.525 kb on - strand, within EX31_RS04355 at 400.536 kb on - strand, within EX31_RS04355 at 400.536 kb on - strand, within EX31_RS04355 at 400.537 kb on + strand, within EX31_RS04355 at 400.538 kb on - strand, within EX31_RS04355 at 400.540 kb on - strand, within EX31_RS04355 at 400.617 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 398,466 + EX31_RS04340 0.70 +1.0 398,558 + EX31_RS04340 0.77 +0.5 398,631 + EX31_RS04340 0.83 +0.5 398,649 + EX31_RS04340 0.85 +0.7 398,658 + EX31_RS04340 0.85 -1.2 398,684 - EX31_RS04340 0.88 +0.5 398,685 + EX31_RS04340 0.88 -1.1 398,691 + EX31_RS04340 0.88 +1.0 398,803 + +1.1 398,833 - +0.2 398,852 - +1.4 398,858 - -1.2 398,861 + +0.3 398,862 - +0.2 398,874 + -0.5 398,974 + +1.4 399,070 - +0.2 399,566 + EX31_RS25140 0.57 -1.6 399,578 - EX31_RS25140 0.63 +1.2 399,609 + EX31_RS25140 0.78 -2.6 399,699 - +1.5 399,704 + -0.2 399,705 - +0.1 399,709 - +1.4 399,726 - +0.9 399,726 - +0.8 399,802 - +0.4 399,802 - +0.6 399,802 - +0.4 399,813 + +1.0 399,813 + -0.1 399,814 - +0.4 399,891 - +2.0 399,909 + EX31_RS04355 0.11 -0.1 399,909 + EX31_RS04355 0.11 +0.3 399,987 + EX31_RS04355 0.21 +0.1 399,999 + EX31_RS04355 0.22 -1.2 400,090 - EX31_RS04355 0.33 -0.9 400,204 - EX31_RS04355 0.48 -0.3 400,271 + EX31_RS04355 0.56 +1.0 400,271 + EX31_RS04355 0.56 -1.0 400,385 + EX31_RS04355 0.70 -0.0 400,385 + EX31_RS04355 0.70 +1.4 400,386 - EX31_RS04355 0.70 -0.2 400,386 - EX31_RS04355 0.70 +0.8 400,503 - EX31_RS04355 0.84 +0.5 400,525 - EX31_RS04355 0.87 -0.5 400,536 - EX31_RS04355 0.89 +1.8 400,536 - EX31_RS04355 0.89 +0.1 400,537 + EX31_RS04355 0.89 +0.2 400,538 - EX31_RS04355 0.89 +0.7 400,540 - EX31_RS04355 0.89 -2.2 400,617 + -0.2
Or see this region's nucleotide sequence