Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS24060 and EX31_RS24065 are separated by 31 nucleotides EX31_RS24065 and EX31_RS24070 are separated by 30 nucleotides EX31_RS24070 and EX31_RS24075 overlap by 16 nucleotides
EX31_RS24060: EX31_RS24060 - pyrimidine utilization transport protein G, at 581,496 to 582,845
_RS24060
EX31_RS24065: EX31_RS24065 - pyrimidine utilization flavin reductase protein F, at 582,877 to 583,503
_RS24065
EX31_RS24070: EX31_RS24070 - malonic semialdehyde reductase, at 583,534 to 584,124
_RS24070
EX31_RS24075: EX31_RS24075 - pyrimidine utilization protein D, at 584,109 to 584,948
_RS24075
Position (kb) 582
583
584 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 581.885 kb on - strand, within EX31_RS24060 at 581.923 kb on + strand, within EX31_RS24060 at 581.923 kb on + strand, within EX31_RS24060 at 581.924 kb on - strand, within EX31_RS24060 at 581.961 kb on + strand, within EX31_RS24060 at 581.961 kb on + strand, within EX31_RS24060 at 582.001 kb on + strand, within EX31_RS24060 at 582.001 kb on + strand, within EX31_RS24060 at 582.001 kb on + strand, within EX31_RS24060 at 582.001 kb on + strand, within EX31_RS24060 at 582.001 kb on + strand, within EX31_RS24060 at 582.001 kb on + strand, within EX31_RS24060 at 582.002 kb on - strand, within EX31_RS24060 at 582.002 kb on - strand, within EX31_RS24060 at 582.002 kb on - strand, within EX31_RS24060 at 582.002 kb on - strand, within EX31_RS24060 at 582.002 kb on - strand, within EX31_RS24060 at 582.042 kb on - strand, within EX31_RS24060 at 582.203 kb on + strand, within EX31_RS24060 at 582.214 kb on + strand, within EX31_RS24060 at 582.214 kb on + strand, within EX31_RS24060 at 582.214 kb on + strand, within EX31_RS24060 at 582.214 kb on + strand, within EX31_RS24060 at 582.215 kb on - strand, within EX31_RS24060 at 582.215 kb on - strand, within EX31_RS24060 at 582.215 kb on - strand, within EX31_RS24060 at 582.215 kb on - strand, within EX31_RS24060 at 582.215 kb on - strand, within EX31_RS24060 at 582.215 kb on - strand, within EX31_RS24060 at 582.215 kb on - strand, within EX31_RS24060 at 582.249 kb on + strand, within EX31_RS24060 at 582.249 kb on + strand, within EX31_RS24060 at 582.474 kb on - strand, within EX31_RS24060 at 582.474 kb on - strand, within EX31_RS24060 at 582.476 kb on - strand, within EX31_RS24060 at 582.494 kb on + strand, within EX31_RS24060 at 582.494 kb on + strand, within EX31_RS24060 at 582.596 kb on - strand, within EX31_RS24060 at 582.697 kb on - strand, within EX31_RS24060 at 582.697 kb on - strand, within EX31_RS24060 at 582.697 kb on - strand, within EX31_RS24060 at 582.725 kb on + strand at 582.725 kb on + strand at 582.726 kb on - strand at 582.726 kb on - strand at 582.726 kb on - strand at 582.726 kb on - strand at 582.744 kb on + strand at 582.745 kb on - strand at 582.745 kb on - strand at 582.745 kb on - strand at 582.812 kb on + strand at 582.812 kb on + strand at 582.836 kb on + strand at 582.837 kb on - strand at 582.837 kb on - strand at 582.837 kb on - strand at 582.856 kb on + strand at 582.856 kb on + strand at 582.856 kb on + strand at 582.857 kb on - strand at 582.859 kb on + strand at 582.859 kb on + strand at 582.859 kb on + strand at 582.859 kb on + strand at 582.859 kb on + strand at 582.859 kb on + strand at 582.860 kb on - strand at 582.860 kb on - strand at 582.879 kb on - strand at 582.923 kb on + strand at 582.923 kb on + strand at 582.923 kb on + strand at 582.924 kb on - strand at 582.955 kb on + strand, within EX31_RS24065 at 583.162 kb on - strand, within EX31_RS24065 at 583.259 kb on - strand, within EX31_RS24065 at 583.535 kb on + strand at 583.627 kb on - strand, within EX31_RS24070 at 583.733 kb on + strand, within EX31_RS24070 at 583.734 kb on - strand, within EX31_RS24070 at 583.823 kb on + strand, within EX31_RS24070 at 583.823 kb on + strand, within EX31_RS24070 at 583.845 kb on - strand, within EX31_RS24070 at 583.957 kb on - strand, within EX31_RS24070 at 584.016 kb on - strand, within EX31_RS24070 at 584.016 kb on - strand, within EX31_RS24070 at 584.087 kb on - strand at 584.087 kb on - strand at 584.087 kb on - strand at 584.157 kb on - strand at 584.249 kb on - strand, within EX31_RS24075 at 584.330 kb on + strand, within EX31_RS24075 at 584.330 kb on + strand, within EX31_RS24075 at 584.331 kb on - strand, within EX31_RS24075
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 581,885 - EX31_RS24060 0.29 +0.4 581,923 + EX31_RS24060 0.32 -0.2 581,923 + EX31_RS24060 0.32 -0.3 581,924 - EX31_RS24060 0.32 -0.3 581,961 + EX31_RS24060 0.34 +0.3 581,961 + EX31_RS24060 0.34 +1.3 582,001 + EX31_RS24060 0.37 -0.6 582,001 + EX31_RS24060 0.37 -0.3 582,001 + EX31_RS24060 0.37 -0.5 582,001 + EX31_RS24060 0.37 -1.7 582,001 + EX31_RS24060 0.37 -0.1 582,001 + EX31_RS24060 0.37 -0.6 582,002 - EX31_RS24060 0.37 +0.7 582,002 - EX31_RS24060 0.37 -0.3 582,002 - EX31_RS24060 0.37 -1.3 582,002 - EX31_RS24060 0.37 -0.1 582,002 - EX31_RS24060 0.37 +0.6 582,042 - EX31_RS24060 0.40 -0.6 582,203 + EX31_RS24060 0.52 +1.0 582,214 + EX31_RS24060 0.53 +0.4 582,214 + EX31_RS24060 0.53 -1.0 582,214 + EX31_RS24060 0.53 -1.5 582,214 + EX31_RS24060 0.53 -0.4 582,215 - EX31_RS24060 0.53 -1.6 582,215 - EX31_RS24060 0.53 +0.0 582,215 - EX31_RS24060 0.53 -0.3 582,215 - EX31_RS24060 0.53 +1.0 582,215 - EX31_RS24060 0.53 +0.1 582,215 - EX31_RS24060 0.53 +0.8 582,215 - EX31_RS24060 0.53 -1.9 582,249 + EX31_RS24060 0.56 +0.3 582,249 + EX31_RS24060 0.56 +0.2 582,474 - EX31_RS24060 0.72 +0.5 582,474 - EX31_RS24060 0.72 +0.7 582,476 - EX31_RS24060 0.73 -1.3 582,494 + EX31_RS24060 0.74 +1.0 582,494 + EX31_RS24060 0.74 -0.8 582,596 - EX31_RS24060 0.81 -0.8 582,697 - EX31_RS24060 0.89 +0.5 582,697 - EX31_RS24060 0.89 +1.1 582,697 - EX31_RS24060 0.89 +0.5 582,725 + -0.4 582,725 + -1.3 582,726 - +1.2 582,726 - -0.8 582,726 - -0.2 582,726 - -0.9 582,744 + -2.5 582,745 - +0.7 582,745 - -0.6 582,745 - +0.8 582,812 + -0.8 582,812 + +0.9 582,836 + -0.0 582,837 - -0.4 582,837 - +1.8 582,837 - -1.6 582,856 + +0.3 582,856 + +1.2 582,856 + +0.1 582,857 - +0.2 582,859 + -0.1 582,859 + -0.3 582,859 + -1.6 582,859 + -0.1 582,859 + -0.5 582,859 + +1.2 582,860 - +0.9 582,860 - +3.2 582,879 - -0.9 582,923 + -1.1 582,923 + +0.5 582,923 + -1.4 582,924 - +0.7 582,955 + EX31_RS24065 0.12 -0.2 583,162 - EX31_RS24065 0.45 -2.0 583,259 - EX31_RS24065 0.61 -0.1 583,535 + +0.8 583,627 - EX31_RS24070 0.16 +0.1 583,733 + EX31_RS24070 0.34 -0.5 583,734 - EX31_RS24070 0.34 +0.7 583,823 + EX31_RS24070 0.49 +0.5 583,823 + EX31_RS24070 0.49 +0.3 583,845 - EX31_RS24070 0.53 +0.5 583,957 - EX31_RS24070 0.72 +0.6 584,016 - EX31_RS24070 0.82 +0.3 584,016 - EX31_RS24070 0.82 +1.0 584,087 - -0.8 584,087 - +0.2 584,087 - +1.0 584,157 - +1.6 584,249 - EX31_RS24075 0.17 +1.2 584,330 + EX31_RS24075 0.26 -0.7 584,330 + EX31_RS24075 0.26 +0.2 584,331 - EX31_RS24075 0.26 +1.9
Or see this region's nucleotide sequence