Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS23830 and EX31_RS23835 are separated by 68 nucleotides EX31_RS23835 and EX31_RS23840 are separated by 160 nucleotides
EX31_RS23830: EX31_RS23830 - phosphonate ABC transporter ATP-binding protein, at 534,058 to 534,894
_RS23830
EX31_RS23835: EX31_RS23835 - phosphonate ABC transporter substrate-binding protein, at 534,963 to 535,895
_RS23835
EX31_RS23840: EX31_RS23840 - phosphonate ABC transporter, permease protein PhnE, at 536,056 to 536,919
_RS23840
Position (kb) 534
535
536 Strain fitness (log2 ratio) -2
-1
0
1
2 at 533.984 kb on - strand at 534.005 kb on - strand at 534.005 kb on - strand at 534.017 kb on + strand at 534.017 kb on + strand at 534.018 kb on - strand at 534.076 kb on - strand at 534.076 kb on - strand at 534.225 kb on - strand, within EX31_RS23830 at 534.247 kb on - strand, within EX31_RS23830 at 534.270 kb on + strand, within EX31_RS23830 at 534.302 kb on + strand, within EX31_RS23830 at 534.302 kb on + strand, within EX31_RS23830 at 534.302 kb on + strand, within EX31_RS23830 at 534.302 kb on + strand, within EX31_RS23830 at 534.302 kb on + strand, within EX31_RS23830 at 534.303 kb on - strand, within EX31_RS23830 at 534.303 kb on - strand, within EX31_RS23830 at 534.303 kb on - strand, within EX31_RS23830 at 534.303 kb on - strand, within EX31_RS23830 at 534.303 kb on - strand, within EX31_RS23830 at 534.303 kb on - strand, within EX31_RS23830 at 534.305 kb on + strand, within EX31_RS23830 at 534.305 kb on + strand, within EX31_RS23830 at 534.305 kb on + strand, within EX31_RS23830 at 534.306 kb on - strand, within EX31_RS23830 at 534.349 kb on - strand, within EX31_RS23830 at 534.444 kb on - strand, within EX31_RS23830 at 534.772 kb on + strand, within EX31_RS23830 at 534.773 kb on - strand, within EX31_RS23830 at 534.837 kb on - strand at 534.860 kb on + strand at 534.860 kb on + strand at 534.861 kb on - strand at 534.862 kb on + strand at 534.863 kb on - strand at 534.863 kb on - strand at 534.863 kb on - strand at 534.910 kb on - strand at 534.924 kb on - strand at 534.929 kb on + strand at 534.960 kb on + strand at 534.960 kb on + strand at 534.960 kb on + strand at 534.961 kb on - strand at 534.961 kb on - strand at 534.976 kb on + strand at 534.977 kb on - strand at 534.994 kb on + strand at 535.048 kb on + strand at 535.048 kb on + strand at 535.054 kb on + strand at 535.054 kb on + strand at 535.054 kb on + strand at 535.054 kb on + strand at 535.055 kb on - strand at 535.154 kb on + strand, within EX31_RS23835 at 535.189 kb on + strand, within EX31_RS23835 at 535.192 kb on + strand, within EX31_RS23835 at 535.192 kb on + strand, within EX31_RS23835 at 535.192 kb on + strand, within EX31_RS23835 at 535.193 kb on - strand, within EX31_RS23835 at 535.193 kb on - strand, within EX31_RS23835 at 535.214 kb on - strand, within EX31_RS23835 at 535.214 kb on - strand, within EX31_RS23835 at 535.214 kb on - strand, within EX31_RS23835 at 535.216 kb on + strand, within EX31_RS23835 at 535.217 kb on - strand, within EX31_RS23835 at 535.217 kb on - strand, within EX31_RS23835 at 535.248 kb on + strand, within EX31_RS23835 at 535.251 kb on - strand, within EX31_RS23835 at 535.254 kb on - strand, within EX31_RS23835 at 535.304 kb on + strand, within EX31_RS23835 at 535.363 kb on + strand, within EX31_RS23835 at 535.477 kb on - strand, within EX31_RS23835 at 535.604 kb on + strand, within EX31_RS23835 at 535.604 kb on + strand, within EX31_RS23835 at 535.604 kb on + strand, within EX31_RS23835 at 535.605 kb on - strand, within EX31_RS23835 at 535.605 kb on - strand, within EX31_RS23835 at 535.670 kb on + strand, within EX31_RS23835 at 535.708 kb on + strand, within EX31_RS23835 at 535.736 kb on + strand, within EX31_RS23835 at 535.736 kb on + strand, within EX31_RS23835 at 535.737 kb on - strand, within EX31_RS23835 at 535.737 kb on - strand, within EX31_RS23835 at 535.737 kb on - strand, within EX31_RS23835 at 535.742 kb on + strand, within EX31_RS23835 at 535.742 kb on + strand, within EX31_RS23835 at 535.796 kb on - strand, within EX31_RS23835 at 535.845 kb on + strand at 535.856 kb on + strand at 535.862 kb on + strand at 535.941 kb on + strand at 536.027 kb on - strand at 536.145 kb on - strand, within EX31_RS23840 at 536.146 kb on + strand, within EX31_RS23840 at 536.218 kb on + strand, within EX31_RS23840 at 536.218 kb on + strand, within EX31_RS23840 at 536.219 kb on - strand, within EX31_RS23840 at 536.220 kb on + strand, within EX31_RS23840 at 536.221 kb on - strand, within EX31_RS23840 at 536.243 kb on + strand, within EX31_RS23840 at 536.258 kb on + strand, within EX31_RS23840 at 536.258 kb on + strand, within EX31_RS23840 at 536.274 kb on + strand, within EX31_RS23840 at 536.275 kb on - strand, within EX31_RS23840 at 536.340 kb on + strand, within EX31_RS23840 at 536.340 kb on + strand, within EX31_RS23840 at 536.340 kb on + strand, within EX31_RS23840 at 536.340 kb on + strand, within EX31_RS23840 at 536.367 kb on - strand, within EX31_RS23840 at 536.449 kb on + strand, within EX31_RS23840 at 536.587 kb on + strand, within EX31_RS23840 at 536.588 kb on - strand, within EX31_RS23840 at 536.588 kb on - strand, within EX31_RS23840 at 536.703 kb on - strand, within EX31_RS23840 at 536.707 kb on + strand, within EX31_RS23840 at 536.707 kb on + strand, within EX31_RS23840 at 536.749 kb on - strand, within EX31_RS23840 at 536.837 kb on + strand at 536.837 kb on + strand at 536.837 kb on + strand at 536.837 kb on + strand at 536.847 kb on + strand at 536.847 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 533,984 - +1.4 534,005 - +1.6 534,005 - +1.0 534,017 + -2.0 534,017 + +0.9 534,018 - -0.5 534,076 - -0.4 534,076 - -0.1 534,225 - EX31_RS23830 0.20 -0.1 534,247 - EX31_RS23830 0.23 -1.9 534,270 + EX31_RS23830 0.25 -0.1 534,302 + EX31_RS23830 0.29 -1.5 534,302 + EX31_RS23830 0.29 +1.2 534,302 + EX31_RS23830 0.29 -1.0 534,302 + EX31_RS23830 0.29 +0.2 534,302 + EX31_RS23830 0.29 +0.1 534,303 - EX31_RS23830 0.29 +0.2 534,303 - EX31_RS23830 0.29 +0.6 534,303 - EX31_RS23830 0.29 +1.2 534,303 - EX31_RS23830 0.29 +1.3 534,303 - EX31_RS23830 0.29 -0.1 534,303 - EX31_RS23830 0.29 -1.5 534,305 + EX31_RS23830 0.30 +0.3 534,305 + EX31_RS23830 0.30 +1.4 534,305 + EX31_RS23830 0.30 +0.1 534,306 - EX31_RS23830 0.30 +0.6 534,349 - EX31_RS23830 0.35 +1.4 534,444 - EX31_RS23830 0.46 -0.7 534,772 + EX31_RS23830 0.85 +1.2 534,773 - EX31_RS23830 0.85 +1.2 534,837 - -2.0 534,860 + -2.1 534,860 + +0.4 534,861 - -0.9 534,862 + +0.7 534,863 - -0.6 534,863 - +1.3 534,863 - +1.0 534,910 - +0.6 534,924 - -0.8 534,929 + +1.1 534,960 + +0.4 534,960 + +1.2 534,960 + +1.2 534,961 - +2.2 534,961 - -0.2 534,976 + +0.5 534,977 - +0.1 534,994 + +1.6 535,048 + +1.2 535,048 + +1.0 535,054 + -0.2 535,054 + +1.6 535,054 + -0.6 535,054 + -0.4 535,055 - -0.1 535,154 + EX31_RS23835 0.20 +0.2 535,189 + EX31_RS23835 0.24 +0.2 535,192 + EX31_RS23835 0.25 +0.8 535,192 + EX31_RS23835 0.25 +0.2 535,192 + EX31_RS23835 0.25 +0.0 535,193 - EX31_RS23835 0.25 -0.8 535,193 - EX31_RS23835 0.25 -0.3 535,214 - EX31_RS23835 0.27 -0.5 535,214 - EX31_RS23835 0.27 +0.7 535,214 - EX31_RS23835 0.27 +0.4 535,216 + EX31_RS23835 0.27 -1.8 535,217 - EX31_RS23835 0.27 +1.6 535,217 - EX31_RS23835 0.27 +0.1 535,248 + EX31_RS23835 0.31 +0.9 535,251 - EX31_RS23835 0.31 +0.5 535,254 - EX31_RS23835 0.31 +1.2 535,304 + EX31_RS23835 0.37 -1.8 535,363 + EX31_RS23835 0.43 +0.5 535,477 - EX31_RS23835 0.55 -0.1 535,604 + EX31_RS23835 0.69 +1.4 535,604 + EX31_RS23835 0.69 +0.6 535,604 + EX31_RS23835 0.69 +1.1 535,605 - EX31_RS23835 0.69 +1.2 535,605 - EX31_RS23835 0.69 +1.0 535,670 + EX31_RS23835 0.76 +0.7 535,708 + EX31_RS23835 0.80 +1.5 535,736 + EX31_RS23835 0.83 +0.4 535,736 + EX31_RS23835 0.83 -0.4 535,737 - EX31_RS23835 0.83 +1.1 535,737 - EX31_RS23835 0.83 -0.6 535,737 - EX31_RS23835 0.83 -1.2 535,742 + EX31_RS23835 0.83 +0.9 535,742 + EX31_RS23835 0.83 +1.2 535,796 - EX31_RS23835 0.89 +2.2 535,845 + +0.3 535,856 + +0.8 535,862 + +0.4 535,941 + +1.2 536,027 - +2.2 536,145 - EX31_RS23840 0.10 -0.6 536,146 + EX31_RS23840 0.10 +1.2 536,218 + EX31_RS23840 0.19 +0.6 536,218 + EX31_RS23840 0.19 -0.3 536,219 - EX31_RS23840 0.19 +2.0 536,220 + EX31_RS23840 0.19 -0.4 536,221 - EX31_RS23840 0.19 -0.2 536,243 + EX31_RS23840 0.22 -0.2 536,258 + EX31_RS23840 0.23 -0.5 536,258 + EX31_RS23840 0.23 +1.8 536,274 + EX31_RS23840 0.25 -1.4 536,275 - EX31_RS23840 0.25 +1.4 536,340 + EX31_RS23840 0.33 -0.1 536,340 + EX31_RS23840 0.33 +0.9 536,340 + EX31_RS23840 0.33 +0.5 536,340 + EX31_RS23840 0.33 -1.7 536,367 - EX31_RS23840 0.36 +1.2 536,449 + EX31_RS23840 0.45 +1.2 536,587 + EX31_RS23840 0.61 -0.3 536,588 - EX31_RS23840 0.62 -0.6 536,588 - EX31_RS23840 0.62 +0.8 536,703 - EX31_RS23840 0.75 +0.4 536,707 + EX31_RS23840 0.75 +1.2 536,707 + EX31_RS23840 0.75 -1.0 536,749 - EX31_RS23840 0.80 -0.1 536,837 + -1.3 536,837 + +0.5 536,837 + -0.1 536,837 + +0.9 536,847 + -0.4 536,847 + -1.7
Or see this region's nucleotide sequence