Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS22730 and EX31_RS22735 are separated by 25 nucleotides EX31_RS22735 and EX31_RS22740 are separated by 145 nucleotides EX31_RS22740 and EX31_RS22745 are separated by 16 nucleotides
EX31_RS22730: EX31_RS22730 - thioredoxin-dependent thiol peroxidase, at 306,407 to 306,874
_RS22730
EX31_RS22735: EX31_RS22735 - glycine cleavage system transcriptional repressor, at 306,900 to 307,466
_RS22735
EX31_RS22740: EX31_RS22740 - 4-hydroxy-tetrahydrodipicolinate synthase, at 307,612 to 308,493
_RS22740
EX31_RS22745: EX31_RS22745 - outer membrane protein assembly factor BamC, at 308,510 to 309,589
_RS22745
Position (kb) 307
308
309 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 306.731 kb on + strand, within EX31_RS22730 at 306.731 kb on + strand, within EX31_RS22730 at 306.732 kb on - strand, within EX31_RS22730 at 306.736 kb on + strand, within EX31_RS22730 at 306.736 kb on + strand, within EX31_RS22730 at 306.737 kb on - strand, within EX31_RS22730 at 306.737 kb on - strand, within EX31_RS22730 at 306.757 kb on + strand, within EX31_RS22730 at 306.757 kb on + strand, within EX31_RS22730 at 306.762 kb on + strand, within EX31_RS22730 at 306.768 kb on + strand, within EX31_RS22730 at 306.768 kb on + strand, within EX31_RS22730 at 306.768 kb on + strand, within EX31_RS22730 at 306.769 kb on - strand, within EX31_RS22730 at 306.769 kb on - strand, within EX31_RS22730 at 306.806 kb on - strand, within EX31_RS22730 at 306.806 kb on - strand, within EX31_RS22730 at 306.806 kb on - strand, within EX31_RS22730 at 306.836 kb on - strand at 306.887 kb on + strand at 306.888 kb on - strand at 306.888 kb on - strand at 306.902 kb on - strand at 306.911 kb on + strand at 306.912 kb on - strand at 306.912 kb on - strand at 306.912 kb on - strand at 306.929 kb on - strand at 306.932 kb on + strand at 306.933 kb on - strand at 306.933 kb on - strand at 306.942 kb on - strand at 306.942 kb on - strand at 306.942 kb on - strand at 306.942 kb on - strand at 306.944 kb on + strand at 306.944 kb on + strand at 306.968 kb on + strand, within EX31_RS22735 at 306.968 kb on + strand, within EX31_RS22735 at 306.968 kb on + strand, within EX31_RS22735 at 306.968 kb on + strand, within EX31_RS22735 at 306.968 kb on + strand, within EX31_RS22735 at 306.969 kb on - strand, within EX31_RS22735 at 306.969 kb on - strand, within EX31_RS22735 at 306.969 kb on - strand, within EX31_RS22735 at 306.969 kb on - strand, within EX31_RS22735 at 306.969 kb on - strand, within EX31_RS22735 at 306.969 kb on - strand, within EX31_RS22735 at 306.969 kb on - strand, within EX31_RS22735 at 306.969 kb on - strand, within EX31_RS22735 at 306.969 kb on - strand, within EX31_RS22735 at 306.969 kb on - strand, within EX31_RS22735 at 306.969 kb on - strand, within EX31_RS22735 at 306.969 kb on - strand, within EX31_RS22735 at 306.969 kb on - strand, within EX31_RS22735 at 306.969 kb on - strand, within EX31_RS22735 at 306.969 kb on - strand, within EX31_RS22735 at 306.972 kb on + strand, within EX31_RS22735 at 306.972 kb on + strand, within EX31_RS22735 at 306.972 kb on + strand, within EX31_RS22735 at 306.972 kb on + strand, within EX31_RS22735 at 306.972 kb on + strand, within EX31_RS22735 at 306.972 kb on + strand, within EX31_RS22735 at 306.972 kb on + strand, within EX31_RS22735 at 306.973 kb on - strand, within EX31_RS22735 at 306.973 kb on - strand, within EX31_RS22735 at 306.973 kb on - strand, within EX31_RS22735 at 306.973 kb on - strand, within EX31_RS22735 at 306.973 kb on - strand, within EX31_RS22735 at 306.973 kb on - strand, within EX31_RS22735 at 306.973 kb on - strand, within EX31_RS22735 at 306.973 kb on - strand, within EX31_RS22735 at 306.973 kb on - strand, within EX31_RS22735 at 306.973 kb on - strand, within EX31_RS22735 at 306.996 kb on - strand, within EX31_RS22735 at 306.996 kb on - strand, within EX31_RS22735 at 307.122 kb on + strand, within EX31_RS22735 at 307.236 kb on + strand, within EX31_RS22735 at 307.236 kb on + strand, within EX31_RS22735 at 307.236 kb on + strand, within EX31_RS22735 at 307.237 kb on - strand, within EX31_RS22735 at 307.283 kb on + strand, within EX31_RS22735 at 307.283 kb on + strand, within EX31_RS22735 at 307.283 kb on + strand, within EX31_RS22735 at 307.284 kb on - strand, within EX31_RS22735 at 307.284 kb on - strand, within EX31_RS22735 at 307.316 kb on + strand, within EX31_RS22735 at 307.317 kb on - strand, within EX31_RS22735 at 307.317 kb on - strand, within EX31_RS22735 at 307.317 kb on - strand, within EX31_RS22735 at 307.317 kb on - strand, within EX31_RS22735 at 307.322 kb on + strand, within EX31_RS22735 at 307.322 kb on + strand, within EX31_RS22735 at 307.323 kb on - strand, within EX31_RS22735 at 307.323 kb on - strand, within EX31_RS22735 at 307.323 kb on - strand, within EX31_RS22735 at 307.323 kb on - strand, within EX31_RS22735 at 307.362 kb on - strand, within EX31_RS22735 at 307.362 kb on - strand, within EX31_RS22735 at 307.362 kb on - strand, within EX31_RS22735 at 307.362 kb on - strand, within EX31_RS22735 at 307.364 kb on + strand, within EX31_RS22735 at 307.364 kb on + strand, within EX31_RS22735 at 307.425 kb on + strand at 307.444 kb on + strand at 307.444 kb on + strand at 307.444 kb on + strand at 307.444 kb on + strand at 307.444 kb on + strand at 307.444 kb on + strand at 307.444 kb on + strand at 307.444 kb on + strand at 307.444 kb on + strand at 307.445 kb on - strand at 307.445 kb on - strand at 307.445 kb on - strand at 307.445 kb on - strand at 307.469 kb on + strand at 307.469 kb on + strand at 307.469 kb on + strand at 307.469 kb on + strand at 307.469 kb on + strand at 307.469 kb on + strand at 307.469 kb on + strand at 307.470 kb on - strand at 307.470 kb on - strand at 307.470 kb on - strand at 307.470 kb on - strand at 307.494 kb on + strand at 307.495 kb on - strand at 307.505 kb on + strand at 307.505 kb on + strand at 307.505 kb on + strand at 307.505 kb on + strand at 307.506 kb on - strand at 307.506 kb on - strand at 307.506 kb on - strand at 307.506 kb on - strand at 307.577 kb on + strand at 307.578 kb on - strand at 307.578 kb on - strand at 307.626 kb on + strand at 307.626 kb on + strand at 307.626 kb on + strand at 307.626 kb on + strand at 307.626 kb on + strand at 307.626 kb on + strand at 307.626 kb on + strand at 307.626 kb on + strand at 307.626 kb on + strand at 307.626 kb on + strand at 307.626 kb on + strand at 307.626 kb on + strand at 307.627 kb on - strand at 307.627 kb on - strand at 307.700 kb on - strand at 307.716 kb on + strand, within EX31_RS22740 at 307.793 kb on + strand, within EX31_RS22740 at 307.793 kb on + strand, within EX31_RS22740 at 307.794 kb on - strand, within EX31_RS22740 at 307.927 kb on + strand, within EX31_RS22740 at 307.927 kb on + strand, within EX31_RS22740 at 307.928 kb on - strand, within EX31_RS22740 at 307.957 kb on + strand, within EX31_RS22740 at 307.957 kb on + strand, within EX31_RS22740 at 308.008 kb on + strand, within EX31_RS22740 at 308.037 kb on + strand, within EX31_RS22740 at 308.037 kb on + strand, within EX31_RS22740 at 308.038 kb on - strand, within EX31_RS22740 at 308.052 kb on + strand, within EX31_RS22740 at 308.079 kb on + strand, within EX31_RS22740 at 308.079 kb on + strand, within EX31_RS22740 at 308.111 kb on + strand, within EX31_RS22740 at 308.111 kb on + strand, within EX31_RS22740 at 308.111 kb on + strand, within EX31_RS22740 at 308.111 kb on + strand, within EX31_RS22740 at 308.111 kb on + strand, within EX31_RS22740 at 308.111 kb on + strand, within EX31_RS22740 at 308.111 kb on + strand, within EX31_RS22740 at 308.111 kb on + strand, within EX31_RS22740 at 308.111 kb on + strand, within EX31_RS22740 at 308.111 kb on + strand, within EX31_RS22740 at 308.111 kb on + strand, within EX31_RS22740 at 308.111 kb on + strand, within EX31_RS22740 at 308.112 kb on - strand, within EX31_RS22740 at 308.122 kb on - strand, within EX31_RS22740 at 308.196 kb on + strand, within EX31_RS22740 at 308.196 kb on + strand, within EX31_RS22740 at 308.197 kb on - strand, within EX31_RS22740 at 308.220 kb on + strand, within EX31_RS22740 at 308.221 kb on - strand, within EX31_RS22740 at 308.240 kb on + strand, within EX31_RS22740 at 308.240 kb on + strand, within EX31_RS22740 at 308.240 kb on + strand, within EX31_RS22740 at 308.240 kb on + strand, within EX31_RS22740 at 308.240 kb on + strand, within EX31_RS22740 at 308.240 kb on + strand, within EX31_RS22740 at 308.240 kb on + strand, within EX31_RS22740 at 308.240 kb on + strand, within EX31_RS22740 at 308.240 kb on + strand, within EX31_RS22740 at 308.240 kb on + strand, within EX31_RS22740 at 308.240 kb on + strand, within EX31_RS22740 at 308.348 kb on + strand, within EX31_RS22740 at 308.348 kb on + strand, within EX31_RS22740 at 308.471 kb on + strand at 308.471 kb on + strand at 308.472 kb on - strand at 308.491 kb on + strand at 308.491 kb on + strand at 308.498 kb on + strand at 308.499 kb on - strand at 308.520 kb on + strand at 308.520 kb on + strand at 308.520 kb on + strand at 308.520 kb on + strand at 308.631 kb on - strand, within EX31_RS22745 at 308.643 kb on + strand, within EX31_RS22745 at 308.643 kb on + strand, within EX31_RS22745 at 308.643 kb on + strand, within EX31_RS22745 at 308.643 kb on + strand, within EX31_RS22745 at 308.644 kb on - strand, within EX31_RS22745 at 308.660 kb on + strand, within EX31_RS22745 at 308.661 kb on - strand, within EX31_RS22745 at 308.732 kb on + strand, within EX31_RS22745 at 308.732 kb on + strand, within EX31_RS22745 at 308.732 kb on + strand, within EX31_RS22745 at 308.732 kb on + strand, within EX31_RS22745 at 308.732 kb on + strand, within EX31_RS22745 at 308.732 kb on + strand, within EX31_RS22745 at 308.732 kb on + strand, within EX31_RS22745 at 308.732 kb on + strand, within EX31_RS22745 at 308.732 kb on + strand, within EX31_RS22745 at 308.732 kb on + strand, within EX31_RS22745 at 308.733 kb on - strand, within EX31_RS22745 at 308.733 kb on - strand, within EX31_RS22745 at 308.733 kb on - strand, within EX31_RS22745 at 308.896 kb on + strand, within EX31_RS22745 at 309.011 kb on + strand, within EX31_RS22745 at 309.050 kb on + strand, within EX31_RS22745 at 309.050 kb on + strand, within EX31_RS22745 at 309.051 kb on - strand, within EX31_RS22745 at 309.131 kb on + strand, within EX31_RS22745 at 309.131 kb on + strand, within EX31_RS22745 at 309.187 kb on + strand, within EX31_RS22745 at 309.187 kb on + strand, within EX31_RS22745 at 309.284 kb on + strand, within EX31_RS22745 at 309.380 kb on + strand, within EX31_RS22745 at 309.381 kb on - strand, within EX31_RS22745 at 309.449 kb on + strand, within EX31_RS22745 at 309.449 kb on + strand, within EX31_RS22745 at 309.481 kb on + strand, within EX31_RS22745 at 309.484 kb on + strand at 309.484 kb on + strand at 309.484 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 306,731 + EX31_RS22730 0.69 +0.6 306,731 + EX31_RS22730 0.69 -0.1 306,732 - EX31_RS22730 0.69 +0.7 306,736 + EX31_RS22730 0.70 +0.9 306,736 + EX31_RS22730 0.70 -0.9 306,737 - EX31_RS22730 0.71 -0.2 306,737 - EX31_RS22730 0.71 +0.0 306,757 + EX31_RS22730 0.75 -0.8 306,757 + EX31_RS22730 0.75 -0.2 306,762 + EX31_RS22730 0.76 -1.3 306,768 + EX31_RS22730 0.77 -0.9 306,768 + EX31_RS22730 0.77 +2.5 306,768 + EX31_RS22730 0.77 +0.0 306,769 - EX31_RS22730 0.77 -0.4 306,769 - EX31_RS22730 0.77 +0.2 306,806 - EX31_RS22730 0.85 -0.3 306,806 - EX31_RS22730 0.85 +0.2 306,806 - EX31_RS22730 0.85 +0.6 306,836 - -0.2 306,887 + +1.0 306,888 - -1.2 306,888 - -0.4 306,902 - -0.2 306,911 + +0.4 306,912 - -0.5 306,912 - +0.7 306,912 - +0.5 306,929 - -0.5 306,932 + -0.7 306,933 - -1.3 306,933 - -0.4 306,942 - +0.6 306,942 - -0.5 306,942 - -0.0 306,942 - +0.4 306,944 + -2.4 306,944 + +0.9 306,968 + EX31_RS22735 0.12 -0.4 306,968 + EX31_RS22735 0.12 -0.6 306,968 + EX31_RS22735 0.12 -1.1 306,968 + EX31_RS22735 0.12 +0.8 306,968 + EX31_RS22735 0.12 -0.7 306,969 - EX31_RS22735 0.12 -0.7 306,969 - EX31_RS22735 0.12 +1.5 306,969 - EX31_RS22735 0.12 -1.2 306,969 - EX31_RS22735 0.12 -1.1 306,969 - EX31_RS22735 0.12 -0.1 306,969 - EX31_RS22735 0.12 -0.4 306,969 - EX31_RS22735 0.12 -0.6 306,969 - EX31_RS22735 0.12 -0.2 306,969 - EX31_RS22735 0.12 -0.9 306,969 - EX31_RS22735 0.12 -1.6 306,969 - EX31_RS22735 0.12 -1.7 306,969 - EX31_RS22735 0.12 -0.4 306,969 - EX31_RS22735 0.12 -1.1 306,969 - EX31_RS22735 0.12 -0.4 306,969 - EX31_RS22735 0.12 -0.3 306,972 + EX31_RS22735 0.13 -0.8 306,972 + EX31_RS22735 0.13 -1.7 306,972 + EX31_RS22735 0.13 +0.2 306,972 + EX31_RS22735 0.13 +0.6 306,972 + EX31_RS22735 0.13 -0.4 306,972 + EX31_RS22735 0.13 -0.8 306,972 + EX31_RS22735 0.13 -1.0 306,973 - EX31_RS22735 0.13 -1.1 306,973 - EX31_RS22735 0.13 -2.6 306,973 - EX31_RS22735 0.13 +0.5 306,973 - EX31_RS22735 0.13 -1.9 306,973 - EX31_RS22735 0.13 -1.7 306,973 - EX31_RS22735 0.13 -1.7 306,973 - EX31_RS22735 0.13 -1.3 306,973 - EX31_RS22735 0.13 +0.6 306,973 - EX31_RS22735 0.13 -1.9 306,973 - EX31_RS22735 0.13 -1.4 306,996 - EX31_RS22735 0.17 -0.4 306,996 - EX31_RS22735 0.17 -1.5 307,122 + EX31_RS22735 0.39 -1.5 307,236 + EX31_RS22735 0.59 +1.2 307,236 + EX31_RS22735 0.59 +0.1 307,236 + EX31_RS22735 0.59 +0.2 307,237 - EX31_RS22735 0.59 -1.1 307,283 + EX31_RS22735 0.68 -1.3 307,283 + EX31_RS22735 0.68 -1.3 307,283 + EX31_RS22735 0.68 -1.1 307,284 - EX31_RS22735 0.68 -2.1 307,284 - EX31_RS22735 0.68 +0.2 307,316 + EX31_RS22735 0.73 +1.9 307,317 - EX31_RS22735 0.74 -0.6 307,317 - EX31_RS22735 0.74 -0.9 307,317 - EX31_RS22735 0.74 -1.7 307,317 - EX31_RS22735 0.74 -2.1 307,322 + EX31_RS22735 0.74 +0.3 307,322 + EX31_RS22735 0.74 -0.3 307,323 - EX31_RS22735 0.75 -1.0 307,323 - EX31_RS22735 0.75 -1.1 307,323 - EX31_RS22735 0.75 -0.8 307,323 - EX31_RS22735 0.75 -0.9 307,362 - EX31_RS22735 0.81 +2.0 307,362 - EX31_RS22735 0.81 -1.1 307,362 - EX31_RS22735 0.81 -1.9 307,362 - EX31_RS22735 0.81 +0.3 307,364 + EX31_RS22735 0.82 +0.8 307,364 + EX31_RS22735 0.82 -0.4 307,425 + -1.8 307,444 + -1.2 307,444 + -1.1 307,444 + -1.4 307,444 + +0.2 307,444 + -1.3 307,444 + -0.6 307,444 + -0.4 307,444 + -0.6 307,444 + +1.5 307,445 - -2.3 307,445 - +0.6 307,445 - -1.6 307,445 - +0.9 307,469 + -0.2 307,469 + +0.0 307,469 + +0.0 307,469 + +1.8 307,469 + +0.9 307,469 + +0.2 307,469 + +2.2 307,470 - +2.2 307,470 - +1.3 307,470 - -1.9 307,470 - -0.7 307,494 + -1.1 307,495 - -0.0 307,505 + -0.6 307,505 + +0.3 307,505 + -0.4 307,505 + -0.1 307,506 - -0.8 307,506 - -0.4 307,506 - -0.1 307,506 - +0.2 307,577 + +0.9 307,578 - -1.1 307,578 - -0.6 307,626 + -2.8 307,626 + -1.3 307,626 + -1.6 307,626 + -1.1 307,626 + -1.7 307,626 + -1.4 307,626 + -3.8 307,626 + -1.6 307,626 + -2.2 307,626 + -2.8 307,626 + +0.6 307,626 + -2.8 307,627 - -1.4 307,627 - -2.9 307,700 - -2.3 307,716 + EX31_RS22740 0.12 -2.4 307,793 + EX31_RS22740 0.21 -3.2 307,793 + EX31_RS22740 0.21 -1.6 307,794 - EX31_RS22740 0.21 -0.4 307,927 + EX31_RS22740 0.36 -0.4 307,927 + EX31_RS22740 0.36 -1.6 307,928 - EX31_RS22740 0.36 -2.9 307,957 + EX31_RS22740 0.39 -1.9 307,957 + EX31_RS22740 0.39 -2.7 308,008 + EX31_RS22740 0.45 -1.8 308,037 + EX31_RS22740 0.48 -1.0 308,037 + EX31_RS22740 0.48 -0.6 308,038 - EX31_RS22740 0.48 +1.2 308,052 + EX31_RS22740 0.50 -0.5 308,079 + EX31_RS22740 0.53 -1.9 308,079 + EX31_RS22740 0.53 -0.5 308,111 + EX31_RS22740 0.57 -1.2 308,111 + EX31_RS22740 0.57 -1.3 308,111 + EX31_RS22740 0.57 -1.9 308,111 + EX31_RS22740 0.57 -0.1 308,111 + EX31_RS22740 0.57 -1.2 308,111 + EX31_RS22740 0.57 -2.0 308,111 + EX31_RS22740 0.57 -1.4 308,111 + EX31_RS22740 0.57 -1.0 308,111 + EX31_RS22740 0.57 -3.0 308,111 + EX31_RS22740 0.57 -3.6 308,111 + EX31_RS22740 0.57 -2.4 308,111 + EX31_RS22740 0.57 -0.7 308,112 - EX31_RS22740 0.57 -1.4 308,122 - EX31_RS22740 0.58 -0.6 308,196 + EX31_RS22740 0.66 -1.9 308,196 + EX31_RS22740 0.66 -2.4 308,197 - EX31_RS22740 0.66 -0.8 308,220 + EX31_RS22740 0.69 +0.5 308,221 - EX31_RS22740 0.69 -2.1 308,240 + EX31_RS22740 0.71 -2.9 308,240 + EX31_RS22740 0.71 -1.3 308,240 + EX31_RS22740 0.71 -1.4 308,240 + EX31_RS22740 0.71 -1.3 308,240 + EX31_RS22740 0.71 -2.4 308,240 + EX31_RS22740 0.71 -1.7 308,240 + EX31_RS22740 0.71 -0.1 308,240 + EX31_RS22740 0.71 -0.2 308,240 + EX31_RS22740 0.71 +0.1 308,240 + EX31_RS22740 0.71 -1.7 308,240 + EX31_RS22740 0.71 -1.6 308,348 + EX31_RS22740 0.83 -1.2 308,348 + EX31_RS22740 0.83 -0.1 308,471 + +0.9 308,471 + -0.0 308,472 - -2.2 308,491 + -0.1 308,491 + +0.7 308,498 + -1.1 308,499 - -1.4 308,520 + +1.3 308,520 + +1.6 308,520 + +0.3 308,520 + -0.5 308,631 - EX31_RS22745 0.11 -2.1 308,643 + EX31_RS22745 0.12 -0.2 308,643 + EX31_RS22745 0.12 -1.3 308,643 + EX31_RS22745 0.12 +1.1 308,643 + EX31_RS22745 0.12 -0.0 308,644 - EX31_RS22745 0.12 -0.6 308,660 + EX31_RS22745 0.14 -0.7 308,661 - EX31_RS22745 0.14 -1.1 308,732 + EX31_RS22745 0.21 -0.8 308,732 + EX31_RS22745 0.21 -0.0 308,732 + EX31_RS22745 0.21 +0.3 308,732 + EX31_RS22745 0.21 -1.5 308,732 + EX31_RS22745 0.21 +0.2 308,732 + EX31_RS22745 0.21 +0.3 308,732 + EX31_RS22745 0.21 -0.2 308,732 + EX31_RS22745 0.21 -0.9 308,732 + EX31_RS22745 0.21 -0.3 308,732 + EX31_RS22745 0.21 +0.1 308,733 - EX31_RS22745 0.21 -0.8 308,733 - EX31_RS22745 0.21 -2.3 308,733 - EX31_RS22745 0.21 -1.6 308,896 + EX31_RS22745 0.36 +1.6 309,011 + EX31_RS22745 0.46 -1.9 309,050 + EX31_RS22745 0.50 -0.2 309,050 + EX31_RS22745 0.50 -0.0 309,051 - EX31_RS22745 0.50 -2.5 309,131 + EX31_RS22745 0.57 +1.1 309,131 + EX31_RS22745 0.57 +0.5 309,187 + EX31_RS22745 0.63 +0.5 309,187 + EX31_RS22745 0.63 -0.9 309,284 + EX31_RS22745 0.72 -0.4 309,380 + EX31_RS22745 0.81 -0.6 309,381 - EX31_RS22745 0.81 -0.4 309,449 + EX31_RS22745 0.87 +0.8 309,449 + EX31_RS22745 0.87 -0.3 309,481 + EX31_RS22745 0.90 -0.9 309,484 + -1.9 309,484 + -1.3 309,484 + -0.5
Or see this region's nucleotide sequence