Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS22240 and EX31_RS22245 are separated by 413 nucleotides EX31_RS22245 and EX31_RS22250 are separated by 205 nucleotides
EX31_RS22240: EX31_RS22240 - trigger factor, at 197,284 to 198,588
_RS22240
EX31_RS22245: EX31_RS22245 - ATP-dependent Clp endopeptidase proteolytic subunit ClpP, at 199,002 to 199,625
_RS22245
EX31_RS22250: EX31_RS22250 - ATP-dependent protease ATP-binding subunit ClpX, at 199,831 to 201,105
_RS22250
Position (kb) 199
200 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 198.133 kb on - strand, within EX31_RS22240 at 198.306 kb on + strand, within EX31_RS22240 at 198.306 kb on + strand, within EX31_RS22240 at 198.445 kb on + strand, within EX31_RS22240 at 198.522 kb on + strand at 198.522 kb on + strand at 198.555 kb on + strand at 198.555 kb on + strand at 198.556 kb on - strand at 198.586 kb on + strand at 198.586 kb on + strand at 198.586 kb on + strand at 198.586 kb on + strand at 198.586 kb on + strand at 198.587 kb on - strand at 198.587 kb on - strand at 198.587 kb on - strand at 198.592 kb on + strand at 198.592 kb on + strand at 198.592 kb on + strand at 198.592 kb on + strand at 198.593 kb on - strand at 198.678 kb on - strand at 198.678 kb on - strand at 198.707 kb on - strand at 198.708 kb on + strand at 198.708 kb on + strand at 198.708 kb on + strand at 198.708 kb on + strand at 198.708 kb on + strand at 198.708 kb on + strand at 198.708 kb on + strand at 198.708 kb on + strand at 198.708 kb on + strand at 198.709 kb on - strand at 198.709 kb on - strand at 198.709 kb on - strand at 198.709 kb on - strand at 198.709 kb on - strand at 198.709 kb on - strand at 198.709 kb on - strand at 198.709 kb on - strand at 198.709 kb on - strand at 198.709 kb on - strand at 198.709 kb on - strand at 198.709 kb on - strand at 198.709 kb on - strand at 198.712 kb on + strand at 198.712 kb on + strand at 198.712 kb on + strand at 198.713 kb on - strand at 198.713 kb on - strand at 198.718 kb on + strand at 198.718 kb on + strand at 198.719 kb on - strand at 198.720 kb on + strand at 198.720 kb on + strand at 198.721 kb on - strand at 198.737 kb on - strand at 198.773 kb on - strand at 198.803 kb on + strand at 198.803 kb on + strand at 198.803 kb on + strand at 198.803 kb on + strand at 198.803 kb on + strand at 198.846 kb on - strand at 198.852 kb on + strand at 198.852 kb on + strand at 198.853 kb on - strand at 198.853 kb on - strand at 198.869 kb on + strand at 198.873 kb on + strand at 198.873 kb on + strand at 198.873 kb on + strand at 198.887 kb on + strand at 198.888 kb on - strand at 198.905 kb on + strand at 198.905 kb on + strand at 198.906 kb on - strand at 198.931 kb on + strand at 198.931 kb on + strand at 198.932 kb on - strand at 198.932 kb on - strand at 198.945 kb on + strand at 198.954 kb on + strand at 198.954 kb on + strand at 198.987 kb on + strand at 198.987 kb on + strand at 198.987 kb on + strand at 198.987 kb on + strand at 198.990 kb on + strand at 198.990 kb on + strand at 199.001 kb on + strand at 199.001 kb on + strand at 199.001 kb on + strand at 199.008 kb on + strand at 199.008 kb on + strand at 199.008 kb on + strand at 199.008 kb on + strand at 199.008 kb on + strand at 199.009 kb on - strand at 199.124 kb on + strand, within EX31_RS22245 at 199.124 kb on + strand, within EX31_RS22245 at 199.124 kb on + strand, within EX31_RS22245 at 199.124 kb on + strand, within EX31_RS22245 at 199.124 kb on + strand, within EX31_RS22245 at 199.157 kb on + strand, within EX31_RS22245 at 199.221 kb on + strand, within EX31_RS22245 at 199.221 kb on + strand, within EX31_RS22245 at 199.221 kb on + strand, within EX31_RS22245 at 199.227 kb on + strand, within EX31_RS22245 at 199.227 kb on + strand, within EX31_RS22245 at 199.227 kb on + strand, within EX31_RS22245 at 199.227 kb on + strand, within EX31_RS22245 at 199.229 kb on + strand, within EX31_RS22245 at 199.262 kb on + strand, within EX31_RS22245 at 199.272 kb on + strand, within EX31_RS22245 at 199.511 kb on + strand, within EX31_RS22245 at 199.534 kb on + strand, within EX31_RS22245 at 199.582 kb on + strand at 199.597 kb on + strand at 199.597 kb on + strand at 199.653 kb on + strand at 199.653 kb on + strand at 199.653 kb on + strand at 199.653 kb on + strand at 199.653 kb on + strand at 199.653 kb on + strand at 199.653 kb on + strand at 199.653 kb on + strand at 199.653 kb on + strand at 199.653 kb on + strand at 199.653 kb on + strand at 199.653 kb on + strand at 199.653 kb on + strand at 199.653 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.655 kb on + strand at 199.656 kb on - strand at 199.656 kb on - strand at 199.656 kb on - strand at 199.656 kb on - strand at 199.659 kb on + strand at 199.659 kb on + strand at 199.659 kb on + strand at 199.659 kb on + strand at 199.659 kb on + strand at 199.659 kb on + strand at 199.659 kb on + strand at 199.659 kb on + strand at 199.659 kb on + strand at 199.659 kb on + strand at 199.659 kb on + strand at 199.659 kb on + strand at 199.659 kb on + strand at 199.659 kb on + strand at 199.659 kb on + strand at 199.660 kb on - strand at 199.660 kb on - strand at 199.661 kb on + strand at 199.661 kb on + strand at 199.661 kb on + strand at 199.661 kb on + strand at 199.661 kb on + strand at 199.723 kb on + strand at 199.723 kb on + strand at 199.766 kb on + strand at 199.766 kb on + strand at 199.766 kb on + strand at 199.766 kb on + strand at 199.767 kb on - strand at 199.769 kb on + strand at 199.769 kb on + strand at 199.769 kb on + strand at 199.769 kb on + strand at 199.769 kb on + strand at 199.769 kb on + strand at 199.769 kb on + strand at 199.769 kb on + strand at 199.771 kb on + strand at 199.771 kb on + strand at 199.771 kb on + strand at 199.771 kb on + strand at 199.771 kb on + strand at 199.771 kb on + strand at 199.771 kb on + strand at 199.788 kb on + strand at 199.839 kb on + strand at 199.839 kb on + strand at 199.870 kb on + strand at 199.870 kb on + strand at 199.870 kb on + strand at 199.908 kb on + strand at 199.933 kb on + strand at 199.933 kb on + strand at 199.933 kb on + strand at 199.934 kb on - strand at 199.935 kb on + strand at 199.958 kb on + strand at 199.962 kb on + strand, within EX31_RS22250 at 199.962 kb on + strand, within EX31_RS22250 at 200.059 kb on + strand, within EX31_RS22250 at 200.116 kb on + strand, within EX31_RS22250 at 200.116 kb on + strand, within EX31_RS22250 at 200.116 kb on + strand, within EX31_RS22250 at 200.118 kb on + strand, within EX31_RS22250 at 200.118 kb on + strand, within EX31_RS22250 at 200.169 kb on + strand, within EX31_RS22250 at 200.250 kb on + strand, within EX31_RS22250 at 200.250 kb on + strand, within EX31_RS22250 at 200.374 kb on + strand, within EX31_RS22250 at 200.374 kb on + strand, within EX31_RS22250 at 200.374 kb on + strand, within EX31_RS22250 at 200.450 kb on + strand, within EX31_RS22250 at 200.450 kb on + strand, within EX31_RS22250 at 200.472 kb on + strand, within EX31_RS22250 at 200.514 kb on + strand, within EX31_RS22250 at 200.514 kb on + strand, within EX31_RS22250 at 200.514 kb on + strand, within EX31_RS22250 at 200.514 kb on + strand, within EX31_RS22250 at 200.547 kb on + strand, within EX31_RS22250 at 200.625 kb on + strand, within EX31_RS22250
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 198,133 - EX31_RS22240 0.65 -0.5 198,306 + EX31_RS22240 0.78 +0.4 198,306 + EX31_RS22240 0.78 -0.2 198,445 + EX31_RS22240 0.89 +0.3 198,522 + -1.1 198,522 + -2.5 198,555 + -0.2 198,555 + +0.8 198,556 - +0.4 198,586 + -0.4 198,586 + +0.7 198,586 + -0.5 198,586 + -0.7 198,586 + -0.3 198,587 - +0.2 198,587 - +0.2 198,587 - +0.6 198,592 + +0.4 198,592 + +0.7 198,592 + -0.5 198,592 + +0.9 198,593 - +1.6 198,678 - -0.1 198,678 - +0.7 198,707 - -0.2 198,708 + +0.6 198,708 + -1.8 198,708 + +1.0 198,708 + -0.4 198,708 + +0.3 198,708 + +2.8 198,708 + -0.1 198,708 + +2.2 198,708 + +0.4 198,709 - -0.4 198,709 - +1.3 198,709 - -0.4 198,709 - -2.0 198,709 - +0.9 198,709 - -1.3 198,709 - +1.8 198,709 - +0.6 198,709 - -0.2 198,709 - -0.9 198,709 - +0.2 198,709 - -1.7 198,709 - +1.6 198,712 + -0.1 198,712 + +0.2 198,712 + -0.6 198,713 - -1.9 198,713 - +1.6 198,718 + +0.3 198,718 + +0.5 198,719 - -1.0 198,720 + +0.3 198,720 + +0.9 198,721 - -0.7 198,737 - +0.5 198,773 - -0.8 198,803 + +0.2 198,803 + -0.3 198,803 + +0.4 198,803 + +0.1 198,803 + +0.0 198,846 - -0.1 198,852 + +0.1 198,852 + +0.3 198,853 - -0.4 198,853 - +0.0 198,869 + +0.4 198,873 + -0.1 198,873 + -0.2 198,873 + +0.6 198,887 + -0.1 198,888 - -1.1 198,905 + +0.8 198,905 + -1.0 198,906 - -1.3 198,931 + -0.4 198,931 + +0.5 198,932 - +1.2 198,932 - -0.4 198,945 + -0.0 198,954 + -0.9 198,954 + +0.4 198,987 + +1.4 198,987 + -0.3 198,987 + -1.3 198,987 + -2.0 198,990 + +0.2 198,990 + -0.9 199,001 + -1.0 199,001 + -2.0 199,001 + -2.0 199,008 + -0.8 199,008 + -1.1 199,008 + -1.1 199,008 + +0.6 199,008 + -1.9 199,009 - -1.3 199,124 + EX31_RS22245 0.20 -1.0 199,124 + EX31_RS22245 0.20 -0.6 199,124 + EX31_RS22245 0.20 -1.7 199,124 + EX31_RS22245 0.20 -1.4 199,124 + EX31_RS22245 0.20 -1.7 199,157 + EX31_RS22245 0.25 -1.7 199,221 + EX31_RS22245 0.35 -1.5 199,221 + EX31_RS22245 0.35 -1.1 199,221 + EX31_RS22245 0.35 -0.1 199,227 + EX31_RS22245 0.36 -1.0 199,227 + EX31_RS22245 0.36 -1.0 199,227 + EX31_RS22245 0.36 -0.1 199,227 + EX31_RS22245 0.36 -0.6 199,229 + EX31_RS22245 0.36 -2.5 199,262 + EX31_RS22245 0.42 -0.6 199,272 + EX31_RS22245 0.43 -0.4 199,511 + EX31_RS22245 0.82 -2.1 199,534 + EX31_RS22245 0.85 -0.1 199,582 + -1.6 199,597 + -0.6 199,597 + -0.7 199,653 + +0.4 199,653 + -1.5 199,653 + +0.9 199,653 + -0.7 199,653 + +0.5 199,653 + -0.2 199,653 + +1.0 199,653 + -1.0 199,653 + -0.1 199,653 + -0.2 199,653 + -1.0 199,653 + +0.4 199,653 + +0.1 199,653 + +1.0 199,655 + -1.5 199,655 + -1.5 199,655 + -2.1 199,655 + -1.4 199,655 + +0.2 199,655 + +0.2 199,655 + +0.2 199,655 + +0.5 199,655 + +0.0 199,655 + -1.0 199,655 + -0.7 199,655 + -0.8 199,655 + -2.2 199,655 + +0.2 199,655 + -0.7 199,655 + +0.2 199,655 + -0.7 199,655 + +0.2 199,655 + -0.3 199,655 + -2.0 199,656 - -1.6 199,656 - -0.3 199,656 - +4.2 199,656 - -0.4 199,659 + +0.3 199,659 + +0.7 199,659 + -0.5 199,659 + +0.2 199,659 + +0.4 199,659 + -1.2 199,659 + -0.3 199,659 + +0.0 199,659 + +0.0 199,659 + +0.5 199,659 + +1.0 199,659 + +0.5 199,659 + +0.3 199,659 + -0.0 199,659 + -0.4 199,660 - -0.1 199,660 - +2.2 199,661 + +0.6 199,661 + -0.4 199,661 + -0.1 199,661 + -0.3 199,661 + -0.4 199,723 + +0.8 199,723 + -0.2 199,766 + +1.8 199,766 + +0.5 199,766 + -1.4 199,766 + -1.0 199,767 - -1.0 199,769 + +0.5 199,769 + -0.7 199,769 + +0.1 199,769 + +1.0 199,769 + +1.0 199,769 + -0.1 199,769 + -0.3 199,769 + -1.1 199,771 + -0.3 199,771 + -0.8 199,771 + -0.9 199,771 + +0.2 199,771 + -0.5 199,771 + -2.4 199,771 + +0.1 199,788 + -1.8 199,839 + -0.4 199,839 + -1.7 199,870 + -0.4 199,870 + -1.0 199,870 + -0.4 199,908 + -0.1 199,933 + -2.7 199,933 + -1.4 199,933 + -1.8 199,934 - +0.6 199,935 + -0.4 199,958 + -0.4 199,962 + EX31_RS22250 0.10 -1.6 199,962 + EX31_RS22250 0.10 -2.3 200,059 + EX31_RS22250 0.18 -1.7 200,116 + EX31_RS22250 0.22 -1.1 200,116 + EX31_RS22250 0.22 -1.9 200,116 + EX31_RS22250 0.22 -0.1 200,118 + EX31_RS22250 0.23 -0.1 200,118 + EX31_RS22250 0.23 -1.3 200,169 + EX31_RS22250 0.27 -1.3 200,250 + EX31_RS22250 0.33 -0.4 200,250 + EX31_RS22250 0.33 -1.7 200,374 + EX31_RS22250 0.43 -2.5 200,374 + EX31_RS22250 0.43 +0.2 200,374 + EX31_RS22250 0.43 -0.7 200,450 + EX31_RS22250 0.49 -1.0 200,450 + EX31_RS22250 0.49 -1.1 200,472 + EX31_RS22250 0.50 -0.6 200,514 + EX31_RS22250 0.54 -1.4 200,514 + EX31_RS22250 0.54 -0.4 200,514 + EX31_RS22250 0.54 -1.6 200,514 + EX31_RS22250 0.54 +0.4 200,547 + EX31_RS22250 0.56 -1.3 200,625 + EX31_RS22250 0.62 -1.0
Or see this region's nucleotide sequence