Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS21795 and EX31_RS21800 are separated by 178 nucleotides EX31_RS21800 and EX31_RS21805 are separated by 30 nucleotides EX31_RS21805 and EX31_RS21810 overlap by 1 nucleotides EX31_RS21810 and EX31_RS21815 are separated by 48 nucleotides
EX31_RS21795: EX31_RS21795 - L-aspartate oxidase, at 101,945 to 103,582
_RS21795
EX31_RS21800: EX31_RS21800 - RNA polymerase sigma factor RpoE, at 103,761 to 104,336
_RS21800
EX31_RS21805: EX31_RS21805 - anti-sigma-E factor RseA, at 104,367 to 105,014
_RS21805
EX31_RS21810: EX31_RS21810 - sigma-E factor regulatory protein RseB, at 105,014 to 105,964
_RS21810
EX31_RS21815: EX31_RS21815 - SoxR-reducing system protein RseC, at 106,013 to 106,489
_RS21815
Position (kb) 104
105
106 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 103.514 kb on + strand at 103.514 kb on + strand at 103.514 kb on + strand at 103.514 kb on + strand at 103.514 kb on + strand at 103.514 kb on + strand at 103.514 kb on + strand at 103.514 kb on + strand at 103.514 kb on + strand at 103.514 kb on + strand at 103.514 kb on + strand at 103.514 kb on + strand at 103.515 kb on - strand at 103.515 kb on - strand at 103.515 kb on - strand at 103.515 kb on - strand at 103.515 kb on - strand at 103.515 kb on - strand at 103.515 kb on - strand at 103.515 kb on - strand at 103.515 kb on - strand at 103.522 kb on + strand at 103.523 kb on - strand at 103.546 kb on + strand at 103.551 kb on + strand at 103.551 kb on + strand at 103.551 kb on + strand at 103.551 kb on + strand at 103.551 kb on + strand at 103.551 kb on + strand at 103.551 kb on + strand at 103.551 kb on + strand at 103.551 kb on + strand at 103.551 kb on + strand at 103.551 kb on + strand at 103.552 kb on - strand at 103.552 kb on - strand at 103.552 kb on - strand at 103.552 kb on - strand at 103.552 kb on - strand at 103.552 kb on - strand at 103.552 kb on - strand at 103.552 kb on - strand at 103.552 kb on - strand at 103.552 kb on - strand at 103.552 kb on - strand at 103.585 kb on + strand at 103.591 kb on - strand at 103.591 kb on - strand at 103.628 kb on + strand at 103.655 kb on + strand at 103.655 kb on + strand at 103.681 kb on + strand at 103.681 kb on + strand at 103.681 kb on + strand at 103.681 kb on + strand at 103.681 kb on + strand at 103.681 kb on + strand at 103.695 kb on + strand at 103.695 kb on + strand at 103.695 kb on + strand at 103.695 kb on + strand at 103.696 kb on - strand at 103.697 kb on + strand at 103.697 kb on + strand at 103.697 kb on + strand at 103.697 kb on + strand at 103.716 kb on + strand at 104.358 kb on + strand at 104.358 kb on + strand at 104.362 kb on + strand at 104.444 kb on + strand, within EX31_RS21805 at 104.494 kb on + strand, within EX31_RS21805 at 104.494 kb on + strand, within EX31_RS21805 at 104.695 kb on + strand, within EX31_RS21805 at 104.695 kb on + strand, within EX31_RS21805 at 104.695 kb on + strand, within EX31_RS21805 at 104.727 kb on + strand, within EX31_RS21805 at 104.729 kb on + strand, within EX31_RS21805 at 104.777 kb on + strand, within EX31_RS21805 at 104.786 kb on + strand, within EX31_RS21805 at 104.873 kb on + strand, within EX31_RS21805 at 104.873 kb on + strand, within EX31_RS21805 at 104.873 kb on + strand, within EX31_RS21805 at 104.873 kb on + strand, within EX31_RS21805 at 104.951 kb on + strand at 105.061 kb on + strand at 105.061 kb on + strand at 105.102 kb on + strand at 105.140 kb on + strand, within EX31_RS21810 at 105.141 kb on - strand, within EX31_RS21810 at 105.163 kb on + strand, within EX31_RS21810 at 105.287 kb on + strand, within EX31_RS21810 at 105.288 kb on - strand, within EX31_RS21810 at 105.303 kb on + strand, within EX31_RS21810 at 105.313 kb on + strand, within EX31_RS21810 at 105.314 kb on - strand, within EX31_RS21810 at 105.331 kb on + strand, within EX31_RS21810 at 105.331 kb on + strand, within EX31_RS21810 at 105.386 kb on + strand, within EX31_RS21810 at 105.386 kb on + strand, within EX31_RS21810 at 105.387 kb on - strand, within EX31_RS21810 at 105.405 kb on + strand, within EX31_RS21810 at 105.453 kb on + strand, within EX31_RS21810 at 105.479 kb on + strand, within EX31_RS21810 at 105.479 kb on + strand, within EX31_RS21810 at 105.480 kb on - strand, within EX31_RS21810 at 105.480 kb on - strand, within EX31_RS21810 at 105.486 kb on + strand, within EX31_RS21810 at 105.486 kb on + strand, within EX31_RS21810 at 105.649 kb on - strand, within EX31_RS21810 at 105.684 kb on + strand, within EX31_RS21810 at 105.684 kb on + strand, within EX31_RS21810 at 105.684 kb on + strand, within EX31_RS21810 at 105.684 kb on + strand, within EX31_RS21810 at 105.742 kb on + strand, within EX31_RS21810 at 105.770 kb on + strand, within EX31_RS21810 at 105.770 kb on + strand, within EX31_RS21810 at 105.770 kb on + strand, within EX31_RS21810 at 105.817 kb on + strand, within EX31_RS21810 at 105.817 kb on + strand, within EX31_RS21810 at 105.817 kb on + strand, within EX31_RS21810 at 105.817 kb on + strand, within EX31_RS21810 at 105.817 kb on + strand, within EX31_RS21810 at 105.817 kb on + strand, within EX31_RS21810 at 105.817 kb on + strand, within EX31_RS21810 at 105.817 kb on + strand, within EX31_RS21810 at 105.817 kb on + strand, within EX31_RS21810 at 105.818 kb on - strand, within EX31_RS21810 at 105.873 kb on + strand at 105.969 kb on + strand at 105.970 kb on - strand at 105.993 kb on + strand at 105.999 kb on + strand at 105.999 kb on + strand at 105.999 kb on + strand at 105.999 kb on + strand at 105.999 kb on + strand at 105.999 kb on + strand at 106.010 kb on + strand at 106.010 kb on + strand at 106.010 kb on + strand at 106.010 kb on + strand at 106.010 kb on + strand at 106.010 kb on + strand at 106.010 kb on + strand at 106.010 kb on + strand at 106.010 kb on + strand at 106.010 kb on + strand at 106.010 kb on + strand at 106.011 kb on - strand at 106.011 kb on - strand at 106.011 kb on - strand at 106.011 kb on - strand at 106.011 kb on - strand at 106.011 kb on - strand at 106.011 kb on - strand at 106.011 kb on - strand at 106.011 kb on - strand at 106.011 kb on - strand at 106.011 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 103,514 + +0.2 103,514 + -0.8 103,514 + -0.2 103,514 + -0.0 103,514 + -0.8 103,514 + -0.9 103,514 + -0.1 103,514 + -1.4 103,514 + +1.1 103,514 + +0.6 103,514 + -1.6 103,514 + -0.9 103,515 - +0.1 103,515 - +0.8 103,515 - -0.2 103,515 - -1.0 103,515 - +0.6 103,515 - -0.8 103,515 - +0.9 103,515 - +0.0 103,515 - +1.2 103,522 + -0.2 103,523 - +0.2 103,546 + -0.8 103,551 + +0.6 103,551 + +1.1 103,551 + -0.7 103,551 + +1.4 103,551 + -0.1 103,551 + -0.2 103,551 + +0.8 103,551 + +0.4 103,551 + +0.1 103,551 + -0.0 103,551 + -2.9 103,552 - +0.9 103,552 - +0.1 103,552 - +0.1 103,552 - -0.9 103,552 - +0.1 103,552 - -1.3 103,552 - +0.6 103,552 - +0.1 103,552 - -2.2 103,552 - +0.1 103,552 - +1.2 103,585 + -0.7 103,591 - -0.6 103,591 - +0.9 103,628 + -0.0 103,655 + +0.3 103,655 + +0.2 103,681 + -0.7 103,681 + -0.8 103,681 + +0.2 103,681 + +1.8 103,681 + -0.7 103,681 + -0.2 103,695 + -1.1 103,695 + +0.3 103,695 + -0.4 103,695 + +0.8 103,696 - -0.1 103,697 + -0.5 103,697 + -0.2 103,697 + -0.0 103,697 + -1.1 103,716 + -0.6 104,358 + -3.5 104,358 + -1.9 104,362 + -1.9 104,444 + EX31_RS21805 0.12 -0.9 104,494 + EX31_RS21805 0.20 -1.7 104,494 + EX31_RS21805 0.20 -2.1 104,695 + EX31_RS21805 0.51 -3.7 104,695 + EX31_RS21805 0.51 -3.6 104,695 + EX31_RS21805 0.51 -2.1 104,727 + EX31_RS21805 0.56 -0.9 104,729 + EX31_RS21805 0.56 -0.4 104,777 + EX31_RS21805 0.63 -2.9 104,786 + EX31_RS21805 0.65 -1.8 104,873 + EX31_RS21805 0.78 -0.6 104,873 + EX31_RS21805 0.78 -0.2 104,873 + EX31_RS21805 0.78 -0.3 104,873 + EX31_RS21805 0.78 +1.5 104,951 + +0.1 105,061 + -1.0 105,061 + +0.9 105,102 + -0.6 105,140 + EX31_RS21810 0.13 -0.9 105,141 - EX31_RS21810 0.13 -1.4 105,163 + EX31_RS21810 0.16 -0.9 105,287 + EX31_RS21810 0.29 +0.6 105,288 - EX31_RS21810 0.29 +0.4 105,303 + EX31_RS21810 0.30 -0.8 105,313 + EX31_RS21810 0.31 +0.6 105,314 - EX31_RS21810 0.32 -0.4 105,331 + EX31_RS21810 0.33 -0.6 105,331 + EX31_RS21810 0.33 -0.4 105,386 + EX31_RS21810 0.39 +0.7 105,386 + EX31_RS21810 0.39 -0.4 105,387 - EX31_RS21810 0.39 -0.5 105,405 + EX31_RS21810 0.41 -0.5 105,453 + EX31_RS21810 0.46 +1.0 105,479 + EX31_RS21810 0.49 -1.9 105,479 + EX31_RS21810 0.49 +0.6 105,480 - EX31_RS21810 0.49 +0.2 105,480 - EX31_RS21810 0.49 -0.8 105,486 + EX31_RS21810 0.50 +1.2 105,486 + EX31_RS21810 0.50 +1.5 105,649 - EX31_RS21810 0.67 +0.1 105,684 + EX31_RS21810 0.70 -0.7 105,684 + EX31_RS21810 0.70 +0.4 105,684 + EX31_RS21810 0.70 +0.2 105,684 + EX31_RS21810 0.70 +0.4 105,742 + EX31_RS21810 0.77 -0.1 105,770 + EX31_RS21810 0.79 -0.5 105,770 + EX31_RS21810 0.79 -0.7 105,770 + EX31_RS21810 0.79 -0.3 105,817 + EX31_RS21810 0.84 +0.0 105,817 + EX31_RS21810 0.84 -0.8 105,817 + EX31_RS21810 0.84 -1.1 105,817 + EX31_RS21810 0.84 -0.6 105,817 + EX31_RS21810 0.84 +0.6 105,817 + EX31_RS21810 0.84 +0.2 105,817 + EX31_RS21810 0.84 -0.2 105,817 + EX31_RS21810 0.84 +0.1 105,817 + EX31_RS21810 0.84 -0.1 105,818 - EX31_RS21810 0.85 +1.1 105,873 + -2.0 105,969 + -0.1 105,970 - +0.3 105,993 + +0.1 105,999 + -0.8 105,999 + -0.5 105,999 + -0.2 105,999 + +0.9 105,999 + -0.4 105,999 + +0.3 106,010 + +0.6 106,010 + +0.4 106,010 + -0.6 106,010 + +0.2 106,010 + +0.3 106,010 + -2.4 106,010 + -0.9 106,010 + -0.5 106,010 + +0.0 106,010 + -0.6 106,010 + -0.4 106,011 - +1.6 106,011 - +1.2 106,011 - -1.1 106,011 - +0.1 106,011 - +0.2 106,011 - -0.0 106,011 - -1.1 106,011 - +1.7 106,011 - -0.7 106,011 - -1.0 106,011 - -1.1
Or see this region's nucleotide sequence