Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS21290 and EX31_RS21295 are separated by 5 nucleotides EX31_RS21295 and EX31_RS21300 are separated by 10 nucleotides EX31_RS21300 and EX31_RS21305 are separated by 3 nucleotides EX31_RS21305 and EX31_RS21310 overlap by 1 nucleotides
EX31_RS21290: EX31_RS21290 - DNA gyrase subunit B, at 900,475 to 901,080
_RS21290
EX31_RS21295: EX31_RS21295 - acyl carrier protein, at 901,086 to 901,340
_RS21295
EX31_RS21300: EX31_RS21300 - acyl carrier protein, at 901,351 to 901,608
_RS21300
EX31_RS21305: EX31_RS21305 - 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 901,612 to 902,424
_RS21305
EX31_RS21310: EX31_RS21310 - beta-ketoacyl synthase chain length factor, at 902,424 to 903,149
_RS21310
Position (kb) 901
902
903 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 900.657 kb on + strand, within EX31_RS21290 at 900.657 kb on + strand, within EX31_RS21290 at 900.658 kb on - strand, within EX31_RS21290 at 900.658 kb on - strand, within EX31_RS21290 at 900.658 kb on - strand, within EX31_RS21290 at 900.658 kb on - strand, within EX31_RS21290 at 900.659 kb on + strand, within EX31_RS21290 at 900.660 kb on - strand, within EX31_RS21290 at 900.660 kb on - strand, within EX31_RS21290 at 900.731 kb on + strand, within EX31_RS21290 at 900.733 kb on + strand, within EX31_RS21290 at 900.734 kb on - strand, within EX31_RS21290 at 900.758 kb on - strand, within EX31_RS21290 at 900.783 kb on - strand, within EX31_RS21290 at 900.786 kb on - strand, within EX31_RS21290 at 900.786 kb on - strand, within EX31_RS21290 at 900.786 kb on - strand, within EX31_RS21290 at 900.796 kb on - strand, within EX31_RS21290 at 900.796 kb on - strand, within EX31_RS21290 at 900.796 kb on - strand, within EX31_RS21290 at 900.974 kb on - strand, within EX31_RS21290 at 900.980 kb on - strand, within EX31_RS21290 at 901.060 kb on - strand at 901.060 kb on - strand at 901.084 kb on + strand at 901.084 kb on + strand at 901.084 kb on + strand at 901.084 kb on + strand at 901.084 kb on + strand at 901.085 kb on - strand at 901.085 kb on - strand at 901.085 kb on - strand at 901.085 kb on - strand at 901.085 kb on - strand at 901.085 kb on - strand at 901.085 kb on - strand at 901.085 kb on - strand at 901.085 kb on - strand at 901.087 kb on + strand at 901.088 kb on - strand at 901.088 kb on - strand at 901.103 kb on + strand at 901.104 kb on - strand at 901.104 kb on - strand at 901.107 kb on + strand at 901.108 kb on - strand at 901.117 kb on - strand, within EX31_RS21295 at 901.117 kb on - strand, within EX31_RS21295 at 901.117 kb on - strand, within EX31_RS21295 at 901.230 kb on + strand, within EX31_RS21295 at 901.231 kb on - strand, within EX31_RS21295 at 901.300 kb on - strand, within EX31_RS21295 at 901.302 kb on + strand, within EX31_RS21295 at 901.303 kb on - strand, within EX31_RS21295 at 901.363 kb on + strand at 901.364 kb on - strand at 901.364 kb on - strand at 901.364 kb on - strand at 901.364 kb on - strand at 901.371 kb on + strand at 901.371 kb on + strand at 901.371 kb on + strand at 901.371 kb on + strand at 901.371 kb on + strand at 901.371 kb on + strand at 901.372 kb on - strand at 901.372 kb on - strand at 901.398 kb on - strand, within EX31_RS21300 at 901.486 kb on - strand, within EX31_RS21300 at 901.576 kb on - strand, within EX31_RS21300 at 901.586 kb on - strand at 901.593 kb on + strand at 901.593 kb on + strand at 901.594 kb on - strand at 901.594 kb on - strand at 901.614 kb on - strand at 901.614 kb on - strand at 901.614 kb on - strand at 901.614 kb on - strand at 901.614 kb on - strand at 901.624 kb on - strand at 901.633 kb on - strand at 901.711 kb on + strand, within EX31_RS21305 at 901.729 kb on + strand, within EX31_RS21305 at 901.730 kb on - strand, within EX31_RS21305 at 901.730 kb on - strand, within EX31_RS21305 at 901.746 kb on + strand, within EX31_RS21305 at 901.903 kb on + strand, within EX31_RS21305 at 901.904 kb on - strand, within EX31_RS21305 at 901.931 kb on - strand, within EX31_RS21305 at 901.996 kb on - strand, within EX31_RS21305 at 901.996 kb on - strand, within EX31_RS21305 at 902.004 kb on - strand, within EX31_RS21305 at 902.017 kb on + strand, within EX31_RS21305 at 902.017 kb on + strand, within EX31_RS21305 at 902.018 kb on - strand, within EX31_RS21305 at 902.030 kb on - strand, within EX31_RS21305 at 902.102 kb on + strand, within EX31_RS21305 at 902.102 kb on + strand, within EX31_RS21305 at 902.103 kb on - strand, within EX31_RS21305 at 902.103 kb on - strand, within EX31_RS21305 at 902.172 kb on + strand, within EX31_RS21305 at 902.299 kb on - strand, within EX31_RS21305 at 902.299 kb on - strand, within EX31_RS21305 at 902.307 kb on + strand, within EX31_RS21305 at 902.308 kb on - strand, within EX31_RS21305 at 902.310 kb on + strand, within EX31_RS21305 at 902.311 kb on - strand, within EX31_RS21305 at 902.311 kb on - strand, within EX31_RS21305 at 902.450 kb on + strand at 902.451 kb on - strand at 902.451 kb on - strand at 902.562 kb on - strand, within EX31_RS21310 at 902.580 kb on - strand, within EX31_RS21310 at 902.595 kb on + strand, within EX31_RS21310 at 902.596 kb on - strand, within EX31_RS21310 at 902.596 kb on - strand, within EX31_RS21310 at 902.596 kb on - strand, within EX31_RS21310 at 902.596 kb on - strand, within EX31_RS21310 at 902.598 kb on + strand, within EX31_RS21310 at 902.605 kb on - strand, within EX31_RS21310 at 902.611 kb on + strand, within EX31_RS21310 at 902.611 kb on + strand, within EX31_RS21310 at 902.612 kb on - strand, within EX31_RS21310 at 902.616 kb on + strand, within EX31_RS21310 at 902.616 kb on + strand, within EX31_RS21310 at 902.617 kb on - strand, within EX31_RS21310 at 902.644 kb on + strand, within EX31_RS21310 at 902.644 kb on + strand, within EX31_RS21310 at 902.644 kb on + strand, within EX31_RS21310 at 902.644 kb on + strand, within EX31_RS21310 at 902.644 kb on + strand, within EX31_RS21310 at 902.644 kb on + strand, within EX31_RS21310 at 902.644 kb on + strand, within EX31_RS21310 at 902.645 kb on - strand, within EX31_RS21310 at 902.645 kb on - strand, within EX31_RS21310 at 902.645 kb on - strand, within EX31_RS21310 at 902.645 kb on - strand, within EX31_RS21310 at 902.646 kb on + strand, within EX31_RS21310 at 902.646 kb on + strand, within EX31_RS21310 at 902.646 kb on + strand, within EX31_RS21310 at 902.646 kb on + strand, within EX31_RS21310 at 902.647 kb on - strand, within EX31_RS21310 at 902.647 kb on - strand, within EX31_RS21310 at 902.647 kb on - strand, within EX31_RS21310 at 902.647 kb on - strand, within EX31_RS21310 at 902.647 kb on - strand, within EX31_RS21310 at 902.647 kb on - strand, within EX31_RS21310 at 902.647 kb on - strand, within EX31_RS21310 at 902.648 kb on + strand, within EX31_RS21310 at 902.648 kb on + strand, within EX31_RS21310 at 902.649 kb on - strand, within EX31_RS21310 at 902.757 kb on + strand, within EX31_RS21310 at 902.758 kb on - strand, within EX31_RS21310 at 902.789 kb on + strand, within EX31_RS21310 at 902.789 kb on + strand, within EX31_RS21310 at 902.813 kb on + strand, within EX31_RS21310 at 902.814 kb on - strand, within EX31_RS21310 at 902.816 kb on + strand, within EX31_RS21310 at 902.816 kb on + strand, within EX31_RS21310 at 902.817 kb on - strand, within EX31_RS21310 at 902.817 kb on - strand, within EX31_RS21310 at 902.817 kb on - strand, within EX31_RS21310 at 902.817 kb on - strand, within EX31_RS21310 at 902.820 kb on + strand, within EX31_RS21310 at 902.820 kb on + strand, within EX31_RS21310 at 902.888 kb on + strand, within EX31_RS21310 at 902.888 kb on + strand, within EX31_RS21310 at 902.931 kb on + strand, within EX31_RS21310 at 902.931 kb on + strand, within EX31_RS21310 at 902.931 kb on + strand, within EX31_RS21310 at 902.932 kb on - strand, within EX31_RS21310 at 902.932 kb on - strand, within EX31_RS21310 at 902.932 kb on - strand, within EX31_RS21310 at 902.932 kb on - strand, within EX31_RS21310 at 902.932 kb on - strand, within EX31_RS21310 at 902.932 kb on - strand, within EX31_RS21310 at 902.932 kb on - strand, within EX31_RS21310 at 902.932 kb on - strand, within EX31_RS21310 at 902.932 kb on - strand, within EX31_RS21310 at 902.940 kb on - strand, within EX31_RS21310 at 903.050 kb on + strand, within EX31_RS21310 at 903.096 kb on - strand at 903.129 kb on + strand at 903.130 kb on - strand at 903.275 kb on + strand at 903.278 kb on + strand at 903.278 kb on + strand at 903.385 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 900,657 + EX31_RS21290 0.30 +0.8 900,657 + EX31_RS21290 0.30 +0.1 900,658 - EX31_RS21290 0.30 +0.2 900,658 - EX31_RS21290 0.30 -0.8 900,658 - EX31_RS21290 0.30 +0.8 900,658 - EX31_RS21290 0.30 -1.7 900,659 + EX31_RS21290 0.30 +0.8 900,660 - EX31_RS21290 0.31 -0.7 900,660 - EX31_RS21290 0.31 +0.4 900,731 + EX31_RS21290 0.42 +1.0 900,733 + EX31_RS21290 0.43 +0.9 900,734 - EX31_RS21290 0.43 -0.9 900,758 - EX31_RS21290 0.47 -0.5 900,783 - EX31_RS21290 0.51 +1.1 900,786 - EX31_RS21290 0.51 -0.2 900,786 - EX31_RS21290 0.51 +0.5 900,786 - EX31_RS21290 0.51 -0.8 900,796 - EX31_RS21290 0.53 -0.3 900,796 - EX31_RS21290 0.53 -0.2 900,796 - EX31_RS21290 0.53 +0.9 900,974 - EX31_RS21290 0.82 +0.9 900,980 - EX31_RS21290 0.83 +1.0 901,060 - +0.3 901,060 - +0.9 901,084 + +1.0 901,084 + +0.8 901,084 + -0.1 901,084 + -1.0 901,084 + +0.1 901,085 - +0.2 901,085 - +1.8 901,085 - +0.1 901,085 - +0.0 901,085 - +0.4 901,085 - -0.2 901,085 - +0.3 901,085 - -0.6 901,085 - -2.0 901,087 + +0.4 901,088 - +0.0 901,088 - -1.1 901,103 + +0.4 901,104 - +0.8 901,104 - -0.4 901,107 + +1.6 901,108 - +1.1 901,117 - EX31_RS21295 0.12 -0.6 901,117 - EX31_RS21295 0.12 -0.0 901,117 - EX31_RS21295 0.12 -0.6 901,230 + EX31_RS21295 0.56 -0.1 901,231 - EX31_RS21295 0.57 +0.8 901,300 - EX31_RS21295 0.84 +1.3 901,302 + EX31_RS21295 0.85 +0.5 901,303 - EX31_RS21295 0.85 +1.4 901,363 + -0.1 901,364 - -0.8 901,364 - +0.0 901,364 - +0.3 901,364 - +0.5 901,371 + -0.8 901,371 + +0.8 901,371 + +0.3 901,371 + -0.2 901,371 + +0.5 901,371 + -1.1 901,372 - +1.8 901,372 - -0.2 901,398 - EX31_RS21300 0.18 +0.7 901,486 - EX31_RS21300 0.52 +0.6 901,576 - EX31_RS21300 0.87 -1.1 901,586 - -0.3 901,593 + -0.5 901,593 + +2.3 901,594 - +0.3 901,594 - +0.7 901,614 - +1.4 901,614 - -0.1 901,614 - +0.8 901,614 - +0.8 901,614 - -0.6 901,624 - -0.4 901,633 - +0.2 901,711 + EX31_RS21305 0.12 -0.1 901,729 + EX31_RS21305 0.14 -1.2 901,730 - EX31_RS21305 0.15 -0.0 901,730 - EX31_RS21305 0.15 +0.5 901,746 + EX31_RS21305 0.16 +1.9 901,903 + EX31_RS21305 0.36 +0.4 901,904 - EX31_RS21305 0.36 -1.8 901,931 - EX31_RS21305 0.39 -0.7 901,996 - EX31_RS21305 0.47 -1.0 901,996 - EX31_RS21305 0.47 +0.4 902,004 - EX31_RS21305 0.48 -1.2 902,017 + EX31_RS21305 0.50 +0.0 902,017 + EX31_RS21305 0.50 -0.4 902,018 - EX31_RS21305 0.50 +0.5 902,030 - EX31_RS21305 0.51 +0.3 902,102 + EX31_RS21305 0.60 +0.7 902,102 + EX31_RS21305 0.60 -1.0 902,103 - EX31_RS21305 0.60 -1.1 902,103 - EX31_RS21305 0.60 +0.2 902,172 + EX31_RS21305 0.69 -0.8 902,299 - EX31_RS21305 0.85 +1.5 902,299 - EX31_RS21305 0.85 +1.1 902,307 + EX31_RS21305 0.85 +2.0 902,308 - EX31_RS21305 0.86 -0.6 902,310 + EX31_RS21305 0.86 -0.8 902,311 - EX31_RS21305 0.86 -0.4 902,311 - EX31_RS21305 0.86 +0.3 902,450 + -0.8 902,451 - -2.2 902,451 - -0.6 902,562 - EX31_RS21310 0.19 -1.4 902,580 - EX31_RS21310 0.21 +0.8 902,595 + EX31_RS21310 0.24 -1.4 902,596 - EX31_RS21310 0.24 +0.8 902,596 - EX31_RS21310 0.24 +0.6 902,596 - EX31_RS21310 0.24 +0.6 902,596 - EX31_RS21310 0.24 -1.5 902,598 + EX31_RS21310 0.24 +1.8 902,605 - EX31_RS21310 0.25 +0.1 902,611 + EX31_RS21310 0.26 -0.7 902,611 + EX31_RS21310 0.26 -2.7 902,612 - EX31_RS21310 0.26 +1.1 902,616 + EX31_RS21310 0.26 +0.3 902,616 + EX31_RS21310 0.26 +1.2 902,617 - EX31_RS21310 0.27 -0.7 902,644 + EX31_RS21310 0.30 -0.1 902,644 + EX31_RS21310 0.30 +0.4 902,644 + EX31_RS21310 0.30 -0.2 902,644 + EX31_RS21310 0.30 +0.9 902,644 + EX31_RS21310 0.30 -0.6 902,644 + EX31_RS21310 0.30 +0.0 902,644 + EX31_RS21310 0.30 -1.0 902,645 - EX31_RS21310 0.30 +2.9 902,645 - EX31_RS21310 0.30 -1.5 902,645 - EX31_RS21310 0.30 +0.5 902,645 - EX31_RS21310 0.30 +1.5 902,646 + EX31_RS21310 0.31 -0.0 902,646 + EX31_RS21310 0.31 +0.2 902,646 + EX31_RS21310 0.31 +0.2 902,646 + EX31_RS21310 0.31 +1.7 902,647 - EX31_RS21310 0.31 +0.0 902,647 - EX31_RS21310 0.31 +0.1 902,647 - EX31_RS21310 0.31 -0.8 902,647 - EX31_RS21310 0.31 +1.3 902,647 - EX31_RS21310 0.31 -0.7 902,647 - EX31_RS21310 0.31 -0.2 902,647 - EX31_RS21310 0.31 -0.3 902,648 + EX31_RS21310 0.31 +0.1 902,648 + EX31_RS21310 0.31 +0.9 902,649 - EX31_RS21310 0.31 -0.6 902,757 + EX31_RS21310 0.46 -0.6 902,758 - EX31_RS21310 0.46 +0.2 902,789 + EX31_RS21310 0.50 +0.6 902,789 + EX31_RS21310 0.50 -0.4 902,813 + EX31_RS21310 0.54 -0.1 902,814 - EX31_RS21310 0.54 -0.5 902,816 + EX31_RS21310 0.54 -0.6 902,816 + EX31_RS21310 0.54 +1.9 902,817 - EX31_RS21310 0.54 -0.5 902,817 - EX31_RS21310 0.54 +1.5 902,817 - EX31_RS21310 0.54 -2.1 902,817 - EX31_RS21310 0.54 -0.4 902,820 + EX31_RS21310 0.55 -2.2 902,820 + EX31_RS21310 0.55 -1.6 902,888 + EX31_RS21310 0.64 -0.5 902,888 + EX31_RS21310 0.64 +0.8 902,931 + EX31_RS21310 0.70 +0.3 902,931 + EX31_RS21310 0.70 +0.0 902,931 + EX31_RS21310 0.70 +0.5 902,932 - EX31_RS21310 0.70 -0.6 902,932 - EX31_RS21310 0.70 -0.7 902,932 - EX31_RS21310 0.70 -0.1 902,932 - EX31_RS21310 0.70 -0.0 902,932 - EX31_RS21310 0.70 +1.5 902,932 - EX31_RS21310 0.70 +0.9 902,932 - EX31_RS21310 0.70 -0.4 902,932 - EX31_RS21310 0.70 +2.7 902,932 - EX31_RS21310 0.70 -0.8 902,940 - EX31_RS21310 0.71 +1.0 903,050 + EX31_RS21310 0.86 +0.4 903,096 - +0.6 903,129 + +0.5 903,130 - +1.1 903,275 + +0.3 903,278 + -0.1 903,278 + -0.3 903,385 + -0.8
Or see this region's nucleotide sequence