Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS18920 and EX31_RS18925 overlap by 4 nucleotides EX31_RS18925 and EX31_RS18930 are separated by 30 nucleotides EX31_RS18930 and EX31_RS18935 are separated by 58 nucleotides
EX31_RS18920: EX31_RS18920 - ABC transporter permease, at 369,788 to 370,564
_RS18920
EX31_RS18925: EX31_RS18925 - ABC transporter ATP-binding protein, at 370,561 to 371,226
_RS18925
EX31_RS18930: EX31_RS18930 - capsular biosynthesis protein, at 371,257 to 372,249
_RS18930
EX31_RS18935: EX31_RS18935 - sugar phosphotransferase, at 372,308 to 373,756
_RS18935
Position (kb) 371
372
373 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 370.350 kb on + strand, within EX31_RS18920 at 370.395 kb on + strand, within EX31_RS18920 at 370.419 kb on + strand, within EX31_RS18920 at 370.477 kb on - strand, within EX31_RS18920 at 370.509 kb on + strand at 370.527 kb on + strand at 370.531 kb on + strand at 370.531 kb on + strand at 370.593 kb on + strand at 370.615 kb on + strand at 370.654 kb on - strand, within EX31_RS18925 at 370.755 kb on + strand, within EX31_RS18925 at 370.755 kb on + strand, within EX31_RS18925 at 370.755 kb on + strand, within EX31_RS18925 at 370.755 kb on + strand, within EX31_RS18925 at 370.755 kb on + strand, within EX31_RS18925 at 370.761 kb on + strand, within EX31_RS18925 at 370.761 kb on + strand, within EX31_RS18925 at 370.761 kb on + strand, within EX31_RS18925 at 370.761 kb on + strand, within EX31_RS18925 at 370.762 kb on - strand, within EX31_RS18925 at 370.997 kb on - strand, within EX31_RS18925 at 371.000 kb on + strand, within EX31_RS18925 at 371.061 kb on + strand, within EX31_RS18925 at 371.195 kb on + strand at 371.241 kb on - strand at 371.483 kb on + strand, within EX31_RS18930 at 371.544 kb on + strand, within EX31_RS18930 at 371.611 kb on + strand, within EX31_RS18930 at 371.818 kb on + strand, within EX31_RS18930 at 372.018 kb on + strand, within EX31_RS18930 at 372.022 kb on + strand, within EX31_RS18930 at 372.087 kb on + strand, within EX31_RS18930 at 372.113 kb on + strand, within EX31_RS18930 at 372.215 kb on + strand at 372.257 kb on + strand at 372.357 kb on + strand at 372.431 kb on + strand at 372.460 kb on - strand, within EX31_RS18935 at 372.593 kb on + strand, within EX31_RS18935 at 372.601 kb on + strand, within EX31_RS18935 at 372.601 kb on + strand, within EX31_RS18935 at 372.601 kb on + strand, within EX31_RS18935 at 372.601 kb on + strand, within EX31_RS18935 at 372.685 kb on + strand, within EX31_RS18935 at 372.704 kb on + strand, within EX31_RS18935 at 372.812 kb on + strand, within EX31_RS18935 at 372.813 kb on - strand, within EX31_RS18935 at 372.989 kb on + strand, within EX31_RS18935 at 372.989 kb on + strand, within EX31_RS18935 at 373.010 kb on - strand, within EX31_RS18935 at 373.014 kb on - strand, within EX31_RS18935 at 373.018 kb on - strand, within EX31_RS18935 at 373.198 kb on + strand, within EX31_RS18935
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 370,350 + EX31_RS18920 0.72 +0.5 370,395 + EX31_RS18920 0.78 +1.1 370,419 + EX31_RS18920 0.81 +0.7 370,477 - EX31_RS18920 0.89 -0.3 370,509 + -0.6 370,527 + -0.7 370,531 + -0.1 370,531 + -1.4 370,593 + -0.5 370,615 + -0.2 370,654 - EX31_RS18925 0.14 -1.3 370,755 + EX31_RS18925 0.29 +0.2 370,755 + EX31_RS18925 0.29 +0.8 370,755 + EX31_RS18925 0.29 -0.1 370,755 + EX31_RS18925 0.29 -1.3 370,755 + EX31_RS18925 0.29 +0.7 370,761 + EX31_RS18925 0.30 -0.9 370,761 + EX31_RS18925 0.30 +0.9 370,761 + EX31_RS18925 0.30 -0.2 370,761 + EX31_RS18925 0.30 -0.7 370,762 - EX31_RS18925 0.30 +2.2 370,997 - EX31_RS18925 0.65 -0.8 371,000 + EX31_RS18925 0.66 +0.2 371,061 + EX31_RS18925 0.75 +0.7 371,195 + +2.2 371,241 - +0.9 371,483 + EX31_RS18930 0.23 +0.2 371,544 + EX31_RS18930 0.29 +1.2 371,611 + EX31_RS18930 0.36 +0.6 371,818 + EX31_RS18930 0.56 +0.8 372,018 + EX31_RS18930 0.77 +0.5 372,022 + EX31_RS18930 0.77 -0.9 372,087 + EX31_RS18930 0.84 +1.3 372,113 + EX31_RS18930 0.86 +1.6 372,215 + -0.3 372,257 + -0.6 372,357 + -2.2 372,431 + -1.1 372,460 - EX31_RS18935 0.10 -1.1 372,593 + EX31_RS18935 0.20 -0.3 372,601 + EX31_RS18935 0.20 -0.4 372,601 + EX31_RS18935 0.20 -1.6 372,601 + EX31_RS18935 0.20 +0.2 372,601 + EX31_RS18935 0.20 +0.0 372,685 + EX31_RS18935 0.26 +0.7 372,704 + EX31_RS18935 0.27 +0.2 372,812 + EX31_RS18935 0.35 -1.3 372,813 - EX31_RS18935 0.35 -0.3 372,989 + EX31_RS18935 0.47 +0.4 372,989 + EX31_RS18935 0.47 +0.4 373,010 - EX31_RS18935 0.48 +0.7 373,014 - EX31_RS18935 0.49 +3.2 373,018 - EX31_RS18935 0.49 +0.5 373,198 + EX31_RS18935 0.61 -2.8
Or see this region's nucleotide sequence