Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS17950 and EX31_RS17955 are separated by 198 nucleotides EX31_RS17955 and EX31_RS17960 are separated by 137 nucleotides
EX31_RS17950: EX31_RS17950 - ABC transporter substrate-binding protein, at 150,440 to 151,528
_RS17950
EX31_RS17955: EX31_RS17955 - SDR family oxidoreductase, at 151,727 to 152,617
_RS17955
EX31_RS17960: EX31_RS17960 - AraC family transcriptional regulator, at 152,755 to 153,672
_RS17960
Position (kb) 151
152
153 Strain fitness (log2 ratio) -2
-1
0
1
2 at 150.736 kb on - strand, within EX31_RS17950 at 150.736 kb on - strand, within EX31_RS17950 at 150.809 kb on - strand, within EX31_RS17950 at 150.876 kb on - strand, within EX31_RS17950 at 150.876 kb on - strand, within EX31_RS17950 at 150.979 kb on - strand, within EX31_RS17950 at 151.083 kb on + strand, within EX31_RS17950 at 151.083 kb on + strand, within EX31_RS17950 at 151.087 kb on + strand, within EX31_RS17950 at 151.088 kb on - strand, within EX31_RS17950 at 151.088 kb on - strand, within EX31_RS17950 at 151.088 kb on - strand, within EX31_RS17950 at 151.104 kb on + strand, within EX31_RS17950 at 151.104 kb on + strand, within EX31_RS17950 at 151.105 kb on - strand, within EX31_RS17950 at 151.105 kb on - strand, within EX31_RS17950 at 151.105 kb on - strand, within EX31_RS17950 at 151.105 kb on - strand, within EX31_RS17950 at 151.171 kb on + strand, within EX31_RS17950 at 151.171 kb on + strand, within EX31_RS17950 at 151.171 kb on + strand, within EX31_RS17950 at 151.172 kb on - strand, within EX31_RS17950 at 151.172 kb on - strand, within EX31_RS17950 at 151.173 kb on + strand, within EX31_RS17950 at 151.174 kb on - strand, within EX31_RS17950 at 151.271 kb on - strand, within EX31_RS17950 at 151.272 kb on + strand, within EX31_RS17950 at 151.273 kb on - strand, within EX31_RS17950 at 151.273 kb on - strand, within EX31_RS17950 at 151.273 kb on - strand, within EX31_RS17950 at 151.273 kb on - strand, within EX31_RS17950 at 151.281 kb on + strand, within EX31_RS17950 at 151.282 kb on - strand, within EX31_RS17950 at 151.282 kb on - strand, within EX31_RS17950 at 151.308 kb on + strand, within EX31_RS17950 at 151.309 kb on - strand, within EX31_RS17950 at 151.309 kb on - strand, within EX31_RS17950 at 151.329 kb on + strand, within EX31_RS17950 at 151.386 kb on + strand, within EX31_RS17950 at 151.387 kb on - strand, within EX31_RS17950 at 151.487 kb on + strand at 151.487 kb on + strand at 151.487 kb on + strand at 151.510 kb on + strand at 151.510 kb on + strand at 151.511 kb on - strand at 151.515 kb on - strand at 151.529 kb on + strand at 151.596 kb on + strand at 151.610 kb on + strand at 151.610 kb on + strand at 151.650 kb on + strand at 151.650 kb on + strand at 151.650 kb on + strand at 151.651 kb on - strand at 151.671 kb on - strand at 151.708 kb on + strand at 151.708 kb on + strand at 151.729 kb on - strand at 151.729 kb on - strand at 151.763 kb on + strand at 151.776 kb on - strand at 151.796 kb on - strand at 151.826 kb on - strand, within EX31_RS17955 at 152.068 kb on - strand, within EX31_RS17955 at 152.311 kb on + strand, within EX31_RS17955 at 152.312 kb on - strand, within EX31_RS17955 at 152.337 kb on - strand, within EX31_RS17955 at 152.609 kb on + strand at 152.609 kb on + strand at 152.609 kb on + strand at 152.610 kb on - strand at 152.610 kb on - strand at 152.641 kb on - strand at 152.641 kb on - strand at 152.672 kb on + strand at 152.672 kb on + strand at 152.672 kb on + strand at 152.673 kb on - strand at 152.673 kb on - strand at 152.673 kb on - strand at 152.673 kb on - strand at 152.673 kb on - strand at 152.679 kb on + strand at 152.684 kb on + strand at 152.684 kb on + strand at 152.685 kb on - strand at 152.685 kb on - strand at 152.685 kb on - strand at 152.686 kb on + strand at 152.687 kb on - strand at 152.687 kb on - strand at 152.709 kb on + strand at 152.710 kb on - strand at 152.710 kb on - strand at 152.800 kb on + strand at 152.800 kb on + strand at 152.800 kb on + strand at 152.800 kb on + strand at 152.800 kb on + strand at 152.800 kb on + strand at 152.800 kb on + strand at 152.800 kb on + strand at 152.800 kb on + strand at 152.800 kb on + strand at 152.801 kb on - strand at 152.801 kb on - strand at 152.802 kb on + strand at 152.802 kb on + strand at 152.802 kb on + strand at 152.802 kb on + strand at 152.802 kb on + strand at 152.802 kb on + strand at 152.802 kb on + strand at 152.803 kb on - strand at 152.803 kb on - strand at 152.803 kb on - strand at 152.803 kb on - strand at 152.803 kb on - strand at 152.803 kb on - strand at 152.803 kb on - strand at 152.857 kb on + strand, within EX31_RS17960 at 152.868 kb on + strand, within EX31_RS17960 at 152.875 kb on - strand, within EX31_RS17960 at 153.126 kb on + strand, within EX31_RS17960 at 153.126 kb on + strand, within EX31_RS17960 at 153.127 kb on - strand, within EX31_RS17960 at 153.159 kb on + strand, within EX31_RS17960 at 153.159 kb on + strand, within EX31_RS17960 at 153.159 kb on + strand, within EX31_RS17960 at 153.160 kb on - strand, within EX31_RS17960 at 153.160 kb on - strand, within EX31_RS17960 at 153.160 kb on - strand, within EX31_RS17960 at 153.160 kb on - strand, within EX31_RS17960 at 153.160 kb on - strand, within EX31_RS17960 at 153.160 kb on - strand, within EX31_RS17960 at 153.160 kb on - strand, within EX31_RS17960 at 153.249 kb on + strand, within EX31_RS17960 at 153.249 kb on + strand, within EX31_RS17960 at 153.452 kb on + strand, within EX31_RS17960 at 153.452 kb on + strand, within EX31_RS17960 at 153.499 kb on + strand, within EX31_RS17960 at 153.500 kb on - strand, within EX31_RS17960 at 153.547 kb on - strand, within EX31_RS17960 at 153.549 kb on + strand, within EX31_RS17960
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 150,736 - EX31_RS17950 0.27 +1.1 150,736 - EX31_RS17950 0.27 -1.3 150,809 - EX31_RS17950 0.34 +0.7 150,876 - EX31_RS17950 0.40 +1.3 150,876 - EX31_RS17950 0.40 +0.1 150,979 - EX31_RS17950 0.49 -0.8 151,083 + EX31_RS17950 0.59 +0.1 151,083 + EX31_RS17950 0.59 +1.1 151,087 + EX31_RS17950 0.59 -0.0 151,088 - EX31_RS17950 0.60 +1.1 151,088 - EX31_RS17950 0.60 -0.0 151,088 - EX31_RS17950 0.60 +0.6 151,104 + EX31_RS17950 0.61 +0.3 151,104 + EX31_RS17950 0.61 +0.8 151,105 - EX31_RS17950 0.61 +1.1 151,105 - EX31_RS17950 0.61 +0.4 151,105 - EX31_RS17950 0.61 +0.1 151,105 - EX31_RS17950 0.61 -0.2 151,171 + EX31_RS17950 0.67 +1.0 151,171 + EX31_RS17950 0.67 -2.0 151,171 + EX31_RS17950 0.67 +1.7 151,172 - EX31_RS17950 0.67 +0.9 151,172 - EX31_RS17950 0.67 +0.8 151,173 + EX31_RS17950 0.67 +0.6 151,174 - EX31_RS17950 0.67 +1.1 151,271 - EX31_RS17950 0.76 -0.6 151,272 + EX31_RS17950 0.76 +1.7 151,273 - EX31_RS17950 0.76 -0.7 151,273 - EX31_RS17950 0.76 +0.4 151,273 - EX31_RS17950 0.76 +0.1 151,273 - EX31_RS17950 0.76 +0.7 151,281 + EX31_RS17950 0.77 -1.0 151,282 - EX31_RS17950 0.77 +0.2 151,282 - EX31_RS17950 0.77 +0.0 151,308 + EX31_RS17950 0.80 -0.8 151,309 - EX31_RS17950 0.80 +0.3 151,309 - EX31_RS17950 0.80 +1.1 151,329 + EX31_RS17950 0.82 -0.6 151,386 + EX31_RS17950 0.87 +1.8 151,387 - EX31_RS17950 0.87 -0.1 151,487 + -0.4 151,487 + +1.1 151,487 + -2.2 151,510 + -0.0 151,510 + +0.9 151,511 - -0.8 151,515 - +0.3 151,529 + -0.2 151,596 + +0.9 151,610 + -0.8 151,610 + -0.2 151,650 + -0.1 151,650 + +0.5 151,650 + +0.6 151,651 - +2.1 151,671 - -0.4 151,708 + -0.3 151,708 + +1.6 151,729 - +0.5 151,729 - +2.1 151,763 + -0.3 151,776 - -2.4 151,796 - -0.3 151,826 - EX31_RS17955 0.11 -1.0 152,068 - EX31_RS17955 0.38 +0.1 152,311 + EX31_RS17955 0.66 -0.7 152,312 - EX31_RS17955 0.66 -2.5 152,337 - EX31_RS17955 0.68 -0.6 152,609 + +1.6 152,609 + +0.8 152,609 + +0.3 152,610 - +0.3 152,610 - +1.9 152,641 - +0.6 152,641 - -0.6 152,672 + +1.5 152,672 + -0.1 152,672 + +1.0 152,673 - -0.1 152,673 - -0.6 152,673 - -0.4 152,673 - -0.2 152,673 - -0.1 152,679 + -1.4 152,684 + -0.4 152,684 + +1.4 152,685 - -0.9 152,685 - -1.7 152,685 - +0.7 152,686 + -0.1 152,687 - -0.3 152,687 - +1.1 152,709 + +0.7 152,710 - -0.3 152,710 - +0.2 152,800 + +0.3 152,800 + +0.3 152,800 + +0.6 152,800 + +1.6 152,800 + -0.4 152,800 + +1.8 152,800 + -0.1 152,800 + +0.8 152,800 + +0.1 152,800 + +0.8 152,801 - -1.1 152,801 - +0.3 152,802 + +0.9 152,802 + +0.3 152,802 + -1.3 152,802 + +0.3 152,802 + +0.1 152,802 + +0.4 152,802 + +1.0 152,803 - +1.6 152,803 - -1.7 152,803 - +1.0 152,803 - +0.3 152,803 - +0.3 152,803 - -0.4 152,803 - +0.3 152,857 + EX31_RS17960 0.11 -0.2 152,868 + EX31_RS17960 0.12 +1.6 152,875 - EX31_RS17960 0.13 -1.4 153,126 + EX31_RS17960 0.40 -1.3 153,126 + EX31_RS17960 0.40 -0.5 153,127 - EX31_RS17960 0.41 +0.8 153,159 + EX31_RS17960 0.44 -2.0 153,159 + EX31_RS17960 0.44 -0.7 153,159 + EX31_RS17960 0.44 -1.7 153,160 - EX31_RS17960 0.44 -0.0 153,160 - EX31_RS17960 0.44 +1.0 153,160 - EX31_RS17960 0.44 +0.7 153,160 - EX31_RS17960 0.44 +0.6 153,160 - EX31_RS17960 0.44 -0.1 153,160 - EX31_RS17960 0.44 -0.2 153,160 - EX31_RS17960 0.44 -0.0 153,249 + EX31_RS17960 0.54 +0.1 153,249 + EX31_RS17960 0.54 +0.7 153,452 + EX31_RS17960 0.76 +0.7 153,452 + EX31_RS17960 0.76 +0.1 153,499 + EX31_RS17960 0.81 +0.2 153,500 - EX31_RS17960 0.81 +1.6 153,547 - EX31_RS17960 0.86 +0.0 153,549 + EX31_RS17960 0.86 +0.2
Or see this region's nucleotide sequence