Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS15090 and EX31_RS15095 overlap by 4 nucleotides EX31_RS15095 and EX31_RS15100 overlap by 17 nucleotides EX31_RS15100 and EX31_RS15105 are separated by 241 nucleotides
EX31_RS15090: EX31_RS15090 - TDP-N-acetylfucosamine:lipid II N-acetylfucosaminyltransferase, at 34,905 to 35,987
_RS15090
EX31_RS15095: EX31_RS15095 - O-antigen assembly polymerase, at 35,984 to 37,363
_RS15095
EX31_RS15100: EX31_RS15100 - lipopolysaccharide N-acetylmannosaminouronosyltransferase, at 37,347 to 38,087
_RS15100
EX31_RS15105: EX31_RS15105 - amino acid permease, at 38,329 to 39,723
_RS15105
Position (kb) 35
36
37
38 Strain fitness (log2 ratio) -2
-1
0
1
2 at 35.013 kb on + strand at 35.013 kb on + strand at 35.014 kb on - strand, within EX31_RS15090 at 35.014 kb on - strand, within EX31_RS15090 at 35.014 kb on - strand, within EX31_RS15090 at 35.021 kb on + strand, within EX31_RS15090 at 35.072 kb on + strand, within EX31_RS15090 at 35.204 kb on + strand, within EX31_RS15090 at 35.205 kb on - strand, within EX31_RS15090 at 35.205 kb on - strand, within EX31_RS15090 at 35.247 kb on + strand, within EX31_RS15090 at 35.284 kb on - strand, within EX31_RS15090 at 35.284 kb on - strand, within EX31_RS15090 at 35.312 kb on + strand, within EX31_RS15090 at 35.313 kb on - strand, within EX31_RS15090 at 35.313 kb on - strand, within EX31_RS15090 at 35.374 kb on + strand, within EX31_RS15090 at 35.387 kb on - strand, within EX31_RS15090 at 35.501 kb on + strand, within EX31_RS15090 at 35.501 kb on + strand, within EX31_RS15090 at 35.501 kb on + strand, within EX31_RS15090 at 35.501 kb on + strand, within EX31_RS15090 at 35.501 kb on + strand, within EX31_RS15090 at 35.501 kb on + strand, within EX31_RS15090 at 35.501 kb on + strand, within EX31_RS15090 at 35.502 kb on - strand, within EX31_RS15090 at 35.502 kb on - strand, within EX31_RS15090 at 35.502 kb on - strand, within EX31_RS15090 at 35.502 kb on - strand, within EX31_RS15090 at 35.502 kb on - strand, within EX31_RS15090 at 35.589 kb on + strand, within EX31_RS15090 at 35.589 kb on + strand, within EX31_RS15090 at 35.589 kb on + strand, within EX31_RS15090 at 35.589 kb on + strand, within EX31_RS15090 at 35.589 kb on + strand, within EX31_RS15090 at 35.589 kb on + strand, within EX31_RS15090 at 35.589 kb on + strand, within EX31_RS15090 at 35.589 kb on + strand, within EX31_RS15090 at 35.589 kb on + strand, within EX31_RS15090 at 35.589 kb on + strand, within EX31_RS15090 at 35.589 kb on + strand, within EX31_RS15090 at 35.589 kb on + strand, within EX31_RS15090 at 35.590 kb on - strand, within EX31_RS15090 at 35.590 kb on - strand, within EX31_RS15090 at 35.590 kb on - strand, within EX31_RS15090 at 35.590 kb on - strand, within EX31_RS15090 at 35.590 kb on - strand, within EX31_RS15090 at 35.590 kb on - strand, within EX31_RS15090 at 35.590 kb on - strand, within EX31_RS15090 at 35.590 kb on - strand, within EX31_RS15090 at 35.676 kb on + strand, within EX31_RS15090 at 35.677 kb on - strand, within EX31_RS15090 at 35.677 kb on - strand, within EX31_RS15090 at 35.710 kb on - strand, within EX31_RS15090 at 35.715 kb on - strand, within EX31_RS15090 at 35.715 kb on - strand, within EX31_RS15090 at 35.841 kb on + strand, within EX31_RS15090 at 35.841 kb on + strand, within EX31_RS15090 at 35.842 kb on - strand, within EX31_RS15090 at 35.842 kb on - strand, within EX31_RS15090 at 35.842 kb on - strand, within EX31_RS15090 at 35.842 kb on - strand, within EX31_RS15090 at 35.842 kb on - strand, within EX31_RS15090 at 35.934 kb on + strand at 35.934 kb on + strand at 35.935 kb on - strand at 35.959 kb on + strand at 35.959 kb on + strand at 36.111 kb on - strand at 36.111 kb on - strand at 36.236 kb on + strand, within EX31_RS15095 at 36.237 kb on - strand, within EX31_RS15095 at 36.377 kb on - strand, within EX31_RS15095 at 36.716 kb on - strand, within EX31_RS15095 at 37.113 kb on - strand, within EX31_RS15095 at 37.225 kb on - strand, within EX31_RS15095 at 37.287 kb on + strand at 37.287 kb on + strand at 37.287 kb on + strand at 37.287 kb on + strand at 37.287 kb on + strand at 37.288 kb on - strand at 37.288 kb on - strand at 37.361 kb on + strand at 37.362 kb on - strand at 37.362 kb on - strand at 37.375 kb on - strand at 37.412 kb on + strand at 37.477 kb on + strand, within EX31_RS15100 at 37.478 kb on - strand, within EX31_RS15100 at 37.478 kb on - strand, within EX31_RS15100 at 37.524 kb on - strand, within EX31_RS15100 at 37.524 kb on - strand, within EX31_RS15100 at 37.524 kb on - strand, within EX31_RS15100 at 37.524 kb on - strand, within EX31_RS15100 at 37.547 kb on + strand, within EX31_RS15100 at 37.547 kb on + strand, within EX31_RS15100 at 37.547 kb on + strand, within EX31_RS15100 at 37.547 kb on + strand, within EX31_RS15100 at 37.548 kb on - strand, within EX31_RS15100 at 37.548 kb on - strand, within EX31_RS15100 at 37.551 kb on + strand, within EX31_RS15100 at 37.580 kb on + strand, within EX31_RS15100 at 37.580 kb on + strand, within EX31_RS15100 at 37.580 kb on + strand, within EX31_RS15100 at 37.581 kb on - strand, within EX31_RS15100 at 37.583 kb on - strand, within EX31_RS15100 at 37.590 kb on - strand, within EX31_RS15100 at 37.590 kb on - strand, within EX31_RS15100 at 37.594 kb on - strand, within EX31_RS15100 at 37.594 kb on - strand, within EX31_RS15100 at 37.594 kb on - strand, within EX31_RS15100 at 37.688 kb on - strand, within EX31_RS15100 at 37.773 kb on + strand, within EX31_RS15100 at 37.773 kb on + strand, within EX31_RS15100 at 37.773 kb on + strand, within EX31_RS15100 at 37.911 kb on + strand, within EX31_RS15100 at 37.911 kb on + strand, within EX31_RS15100 at 37.911 kb on + strand, within EX31_RS15100 at 37.911 kb on + strand, within EX31_RS15100 at 37.911 kb on + strand, within EX31_RS15100 at 37.911 kb on + strand, within EX31_RS15100 at 37.911 kb on + strand, within EX31_RS15100 at 37.911 kb on + strand, within EX31_RS15100 at 37.911 kb on + strand, within EX31_RS15100 at 37.911 kb on + strand, within EX31_RS15100 at 37.911 kb on + strand, within EX31_RS15100 at 37.911 kb on + strand, within EX31_RS15100 at 37.911 kb on + strand, within EX31_RS15100 at 37.912 kb on - strand, within EX31_RS15100 at 37.912 kb on - strand, within EX31_RS15100 at 37.912 kb on - strand, within EX31_RS15100 at 37.912 kb on - strand, within EX31_RS15100 at 37.912 kb on - strand, within EX31_RS15100 at 37.912 kb on - strand, within EX31_RS15100 at 37.912 kb on - strand, within EX31_RS15100 at 37.912 kb on - strand, within EX31_RS15100 at 37.912 kb on - strand, within EX31_RS15100 at 37.913 kb on + strand, within EX31_RS15100 at 37.913 kb on + strand, within EX31_RS15100 at 37.913 kb on + strand, within EX31_RS15100 at 37.913 kb on + strand, within EX31_RS15100 at 37.913 kb on + strand, within EX31_RS15100 at 37.914 kb on - strand, within EX31_RS15100 at 37.914 kb on - strand, within EX31_RS15100 at 37.932 kb on + strand, within EX31_RS15100 at 37.932 kb on + strand, within EX31_RS15100 at 37.932 kb on + strand, within EX31_RS15100 at 37.932 kb on + strand, within EX31_RS15100 at 37.932 kb on + strand, within EX31_RS15100 at 37.932 kb on + strand, within EX31_RS15100 at 37.933 kb on - strand, within EX31_RS15100 at 37.933 kb on - strand, within EX31_RS15100 at 37.933 kb on - strand, within EX31_RS15100 at 37.968 kb on - strand, within EX31_RS15100 at 38.001 kb on + strand, within EX31_RS15100 at 38.015 kb on - strand at 38.057 kb on + strand at 38.064 kb on + strand at 38.067 kb on + strand at 38.067 kb on + strand at 38.067 kb on + strand at 38.067 kb on + strand at 38.067 kb on + strand at 38.067 kb on + strand at 38.068 kb on - strand at 38.068 kb on - strand at 38.068 kb on - strand at 38.068 kb on - strand at 38.068 kb on - strand at 38.068 kb on - strand at 38.068 kb on - strand at 38.068 kb on - strand at 38.068 kb on - strand at 38.070 kb on + strand at 38.070 kb on + strand at 38.071 kb on - strand at 38.085 kb on + strand at 38.085 kb on + strand at 38.086 kb on - strand at 38.088 kb on + strand at 38.088 kb on + strand at 38.088 kb on + strand at 38.088 kb on + strand at 38.088 kb on + strand at 38.088 kb on + strand at 38.088 kb on + strand at 38.088 kb on + strand at 38.088 kb on + strand at 38.088 kb on + strand at 38.089 kb on - strand at 38.089 kb on - strand at 38.089 kb on - strand at 38.089 kb on - strand at 38.123 kb on + strand at 38.123 kb on + strand at 38.124 kb on - strand at 38.124 kb on - strand at 38.124 kb on - strand at 38.173 kb on + strand at 38.174 kb on - strand at 38.231 kb on - strand at 38.249 kb on + strand at 38.249 kb on + strand at 38.249 kb on + strand at 38.249 kb on + strand at 38.249 kb on + strand at 38.249 kb on + strand at 38.249 kb on + strand at 38.249 kb on + strand at 38.249 kb on + strand at 38.250 kb on - strand at 38.250 kb on - strand at 38.250 kb on - strand at 38.250 kb on - strand at 38.250 kb on - strand at 38.250 kb on - strand at 38.250 kb on - strand at 38.250 kb on - strand at 38.250 kb on - strand at 38.251 kb on + strand at 38.251 kb on + strand at 38.251 kb on + strand at 38.252 kb on - strand at 38.269 kb on + strand at 38.276 kb on - strand at 38.309 kb on + strand at 38.328 kb on + strand at 38.328 kb on + strand at 38.328 kb on + strand at 38.357 kb on + strand at 38.357 kb on + strand at 38.358 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 35,013 + -0.5 35,013 + +0.0 35,014 - EX31_RS15090 0.10 +0.4 35,014 - EX31_RS15090 0.10 +1.4 35,014 - EX31_RS15090 0.10 +0.4 35,021 + EX31_RS15090 0.11 -0.3 35,072 + EX31_RS15090 0.15 +0.2 35,204 + EX31_RS15090 0.28 -0.9 35,205 - EX31_RS15090 0.28 -1.1 35,205 - EX31_RS15090 0.28 +0.4 35,247 + EX31_RS15090 0.32 -1.1 35,284 - EX31_RS15090 0.35 -1.0 35,284 - EX31_RS15090 0.35 +1.0 35,312 + EX31_RS15090 0.38 -0.1 35,313 - EX31_RS15090 0.38 -0.1 35,313 - EX31_RS15090 0.38 -0.1 35,374 + EX31_RS15090 0.43 +0.5 35,387 - EX31_RS15090 0.45 +0.1 35,501 + EX31_RS15090 0.55 -0.7 35,501 + EX31_RS15090 0.55 -0.9 35,501 + EX31_RS15090 0.55 +0.3 35,501 + EX31_RS15090 0.55 -0.6 35,501 + EX31_RS15090 0.55 +0.6 35,501 + EX31_RS15090 0.55 -0.3 35,501 + EX31_RS15090 0.55 -0.0 35,502 - EX31_RS15090 0.55 -1.0 35,502 - EX31_RS15090 0.55 +0.5 35,502 - EX31_RS15090 0.55 +0.2 35,502 - EX31_RS15090 0.55 -1.6 35,502 - EX31_RS15090 0.55 +1.3 35,589 + EX31_RS15090 0.63 +0.6 35,589 + EX31_RS15090 0.63 +0.4 35,589 + EX31_RS15090 0.63 -0.7 35,589 + EX31_RS15090 0.63 -2.0 35,589 + EX31_RS15090 0.63 -0.7 35,589 + EX31_RS15090 0.63 +1.5 35,589 + EX31_RS15090 0.63 -1.0 35,589 + EX31_RS15090 0.63 +0.5 35,589 + EX31_RS15090 0.63 +1.6 35,589 + EX31_RS15090 0.63 +0.3 35,589 + EX31_RS15090 0.63 +0.5 35,589 + EX31_RS15090 0.63 -0.2 35,590 - EX31_RS15090 0.63 -0.5 35,590 - EX31_RS15090 0.63 +0.5 35,590 - EX31_RS15090 0.63 +0.3 35,590 - EX31_RS15090 0.63 +0.8 35,590 - EX31_RS15090 0.63 -0.7 35,590 - EX31_RS15090 0.63 +1.2 35,590 - EX31_RS15090 0.63 -0.8 35,590 - EX31_RS15090 0.63 -1.2 35,676 + EX31_RS15090 0.71 +0.1 35,677 - EX31_RS15090 0.71 -0.4 35,677 - EX31_RS15090 0.71 -0.4 35,710 - EX31_RS15090 0.74 +1.3 35,715 - EX31_RS15090 0.75 +0.1 35,715 - EX31_RS15090 0.75 -0.2 35,841 + EX31_RS15090 0.86 +1.0 35,841 + EX31_RS15090 0.86 +0.6 35,842 - EX31_RS15090 0.87 +0.8 35,842 - EX31_RS15090 0.87 +0.2 35,842 - EX31_RS15090 0.87 +0.6 35,842 - EX31_RS15090 0.87 +1.2 35,842 - EX31_RS15090 0.87 -0.5 35,934 + +2.6 35,934 + +0.5 35,935 - -0.3 35,959 + +0.2 35,959 + -0.3 36,111 - -1.0 36,111 - -0.5 36,236 + EX31_RS15095 0.18 +0.6 36,237 - EX31_RS15095 0.18 +0.3 36,377 - EX31_RS15095 0.28 -1.7 36,716 - EX31_RS15095 0.53 +0.5 37,113 - EX31_RS15095 0.82 -0.9 37,225 - EX31_RS15095 0.90 -0.9 37,287 + +0.4 37,287 + -0.1 37,287 + +0.0 37,287 + -0.5 37,287 + -1.0 37,288 - +2.5 37,288 - +0.8 37,361 + +0.2 37,362 - +1.9 37,362 - -0.1 37,375 - +1.3 37,412 + +0.3 37,477 + EX31_RS15100 0.18 -0.3 37,478 - EX31_RS15100 0.18 -0.8 37,478 - EX31_RS15100 0.18 -0.9 37,524 - EX31_RS15100 0.24 -0.1 37,524 - EX31_RS15100 0.24 +0.1 37,524 - EX31_RS15100 0.24 +0.9 37,524 - EX31_RS15100 0.24 -0.2 37,547 + EX31_RS15100 0.27 -0.9 37,547 + EX31_RS15100 0.27 +0.2 37,547 + EX31_RS15100 0.27 -0.4 37,547 + EX31_RS15100 0.27 +0.4 37,548 - EX31_RS15100 0.27 +1.1 37,548 - EX31_RS15100 0.27 -0.7 37,551 + EX31_RS15100 0.28 +0.5 37,580 + EX31_RS15100 0.31 +0.7 37,580 + EX31_RS15100 0.31 +0.7 37,580 + EX31_RS15100 0.31 +0.0 37,581 - EX31_RS15100 0.32 -0.7 37,583 - EX31_RS15100 0.32 -0.2 37,590 - EX31_RS15100 0.33 -1.0 37,590 - EX31_RS15100 0.33 +0.6 37,594 - EX31_RS15100 0.33 +0.5 37,594 - EX31_RS15100 0.33 -0.3 37,594 - EX31_RS15100 0.33 -0.4 37,688 - EX31_RS15100 0.46 -0.4 37,773 + EX31_RS15100 0.57 -0.1 37,773 + EX31_RS15100 0.57 -1.2 37,773 + EX31_RS15100 0.57 -0.2 37,911 + EX31_RS15100 0.76 -0.1 37,911 + EX31_RS15100 0.76 -1.2 37,911 + EX31_RS15100 0.76 +1.0 37,911 + EX31_RS15100 0.76 -1.6 37,911 + EX31_RS15100 0.76 +2.3 37,911 + EX31_RS15100 0.76 +0.5 37,911 + EX31_RS15100 0.76 -0.1 37,911 + EX31_RS15100 0.76 -0.5 37,911 + EX31_RS15100 0.76 +0.1 37,911 + EX31_RS15100 0.76 +0.9 37,911 + EX31_RS15100 0.76 -1.6 37,911 + EX31_RS15100 0.76 +1.2 37,911 + EX31_RS15100 0.76 -1.1 37,912 - EX31_RS15100 0.76 -0.9 37,912 - EX31_RS15100 0.76 -0.8 37,912 - EX31_RS15100 0.76 -0.5 37,912 - EX31_RS15100 0.76 -0.1 37,912 - EX31_RS15100 0.76 -0.4 37,912 - EX31_RS15100 0.76 -0.9 37,912 - EX31_RS15100 0.76 -0.3 37,912 - EX31_RS15100 0.76 +0.3 37,912 - EX31_RS15100 0.76 -0.2 37,913 + EX31_RS15100 0.76 -1.2 37,913 + EX31_RS15100 0.76 -0.9 37,913 + EX31_RS15100 0.76 +1.0 37,913 + EX31_RS15100 0.76 +0.4 37,913 + EX31_RS15100 0.76 +0.2 37,914 - EX31_RS15100 0.77 +0.3 37,914 - EX31_RS15100 0.77 -0.2 37,932 + EX31_RS15100 0.79 -0.0 37,932 + EX31_RS15100 0.79 +0.8 37,932 + EX31_RS15100 0.79 -0.4 37,932 + EX31_RS15100 0.79 +2.6 37,932 + EX31_RS15100 0.79 -0.4 37,932 + EX31_RS15100 0.79 -0.2 37,933 - EX31_RS15100 0.79 +0.7 37,933 - EX31_RS15100 0.79 +0.3 37,933 - EX31_RS15100 0.79 +1.7 37,968 - EX31_RS15100 0.84 -1.4 38,001 + EX31_RS15100 0.88 -0.2 38,015 - -1.6 38,057 + +0.5 38,064 + -0.0 38,067 + -0.0 38,067 + -1.7 38,067 + +0.7 38,067 + +1.0 38,067 + -0.1 38,067 + +0.6 38,068 - -0.7 38,068 - -0.7 38,068 - +0.1 38,068 - -0.0 38,068 - +0.1 38,068 - +0.1 38,068 - +0.6 38,068 - +0.3 38,068 - +0.4 38,070 + -0.4 38,070 + +0.5 38,071 - -1.8 38,085 + +0.4 38,085 + +0.3 38,086 - +0.4 38,088 + +0.4 38,088 + +0.1 38,088 + -0.3 38,088 + +1.9 38,088 + +1.3 38,088 + +0.3 38,088 + +0.4 38,088 + -0.3 38,088 + +0.8 38,088 + +1.0 38,089 - +0.8 38,089 - +0.5 38,089 - +0.4 38,089 - +0.5 38,123 + +0.5 38,123 + -0.2 38,124 - -0.6 38,124 - -0.2 38,124 - +1.0 38,173 + +0.5 38,174 - -0.4 38,231 - -2.0 38,249 + +0.5 38,249 + +1.3 38,249 + +0.9 38,249 + +0.0 38,249 + +0.4 38,249 + +1.2 38,249 + +0.0 38,249 + +0.2 38,249 + -1.0 38,250 - +0.7 38,250 - +0.0 38,250 - +0.4 38,250 - -1.4 38,250 - -0.4 38,250 - -0.6 38,250 - -0.2 38,250 - +0.1 38,250 - -0.7 38,251 + +0.1 38,251 + +0.2 38,251 + -0.0 38,252 - -0.4 38,269 + -0.4 38,276 - +0.1 38,309 + +0.5 38,328 + -0.0 38,328 + +0.3 38,328 + -0.9 38,357 + -0.6 38,357 + +0.4 38,358 - -0.9
Or see this region's nucleotide sequence