Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS12005 and EX31_RS12010 are separated by 206 nucleotides EX31_RS12010 and EX31_RS12015 are separated by 610 nucleotides
EX31_RS12005: EX31_RS12005 - LysR family transcriptional regulator, at 64,901 to 65,755
_RS12005
EX31_RS12010: EX31_RS12010 - cysteine protease, at 65,962 to 66,651
_RS12010
EX31_RS12015: EX31_RS12015 - metallophosphoesterase, at 67,262 to 68,845
_RS12015
Position (kb) 65
66
67 Strain fitness (log2 ratio) -2
-1
0
1
2 at 65.063 kb on + strand, within EX31_RS12005 at 65.064 kb on - strand, within EX31_RS12005 at 65.124 kb on + strand, within EX31_RS12005 at 65.124 kb on + strand, within EX31_RS12005 at 65.131 kb on + strand, within EX31_RS12005 at 65.140 kb on + strand, within EX31_RS12005 at 65.140 kb on + strand, within EX31_RS12005 at 65.141 kb on - strand, within EX31_RS12005 at 65.141 kb on - strand, within EX31_RS12005 at 65.141 kb on - strand, within EX31_RS12005 at 65.141 kb on - strand, within EX31_RS12005 at 65.182 kb on - strand, within EX31_RS12005 at 65.182 kb on - strand, within EX31_RS12005 at 65.390 kb on - strand, within EX31_RS12005 at 65.469 kb on - strand, within EX31_RS12005 at 65.469 kb on - strand, within EX31_RS12005 at 65.661 kb on + strand, within EX31_RS12005 at 65.661 kb on + strand, within EX31_RS12005 at 65.661 kb on + strand, within EX31_RS12005 at 65.662 kb on - strand, within EX31_RS12005 at 65.662 kb on - strand, within EX31_RS12005 at 65.662 kb on - strand, within EX31_RS12005 at 65.662 kb on - strand, within EX31_RS12005 at 65.683 kb on + strand at 65.684 kb on - strand at 65.716 kb on + strand at 65.716 kb on + strand at 65.717 kb on - strand at 65.799 kb on - strand at 65.807 kb on - strand at 65.807 kb on - strand at 65.872 kb on - strand at 65.885 kb on + strand at 65.995 kb on + strand at 65.997 kb on + strand at 66.006 kb on - strand at 66.008 kb on - strand at 66.008 kb on - strand at 66.009 kb on + strand at 66.012 kb on + strand at 66.101 kb on - strand, within EX31_RS12010 at 66.128 kb on - strand, within EX31_RS12010 at 66.169 kb on + strand, within EX31_RS12010 at 66.170 kb on - strand, within EX31_RS12010 at 66.170 kb on - strand, within EX31_RS12010 at 66.208 kb on + strand, within EX31_RS12010 at 66.208 kb on + strand, within EX31_RS12010 at 66.209 kb on - strand, within EX31_RS12010 at 66.209 kb on - strand, within EX31_RS12010 at 66.228 kb on + strand, within EX31_RS12010 at 66.228 kb on + strand, within EX31_RS12010 at 66.467 kb on + strand, within EX31_RS12010 at 66.467 kb on + strand, within EX31_RS12010 at 66.467 kb on + strand, within EX31_RS12010 at 66.547 kb on - strand, within EX31_RS12010 at 66.609 kb on + strand at 66.716 kb on + strand at 66.716 kb on + strand at 66.865 kb on + strand at 66.866 kb on - strand at 66.890 kb on - strand at 66.935 kb on + strand at 66.994 kb on - strand at 66.994 kb on - strand at 67.048 kb on + strand at 67.048 kb on + strand at 67.049 kb on - strand at 67.050 kb on + strand at 67.050 kb on + strand at 67.051 kb on - strand at 67.051 kb on - strand at 67.051 kb on - strand at 67.051 kb on - strand at 67.062 kb on + strand at 67.062 kb on + strand at 67.134 kb on + strand at 67.135 kb on - strand at 67.135 kb on - strand at 67.138 kb on + strand at 67.138 kb on + strand at 67.160 kb on + strand at 67.160 kb on + strand at 67.223 kb on + strand at 67.223 kb on + strand at 67.224 kb on - strand at 67.272 kb on + strand at 67.273 kb on - strand at 67.273 kb on - strand at 67.279 kb on + strand at 67.280 kb on - strand at 67.301 kb on - strand at 67.301 kb on - strand at 67.306 kb on + strand at 67.306 kb on + strand at 67.306 kb on + strand at 67.306 kb on + strand at 67.307 kb on - strand at 67.307 kb on - strand at 67.341 kb on - strand at 67.350 kb on + strand at 67.350 kb on + strand at 67.351 kb on - strand at 67.351 kb on - strand at 67.351 kb on - strand at 67.351 kb on - strand at 67.484 kb on + strand, within EX31_RS12015 at 67.484 kb on + strand, within EX31_RS12015 at 67.484 kb on + strand, within EX31_RS12015 at 67.485 kb on - strand, within EX31_RS12015 at 67.485 kb on - strand, within EX31_RS12015 at 67.485 kb on - strand, within EX31_RS12015 at 67.485 kb on - strand, within EX31_RS12015 at 67.485 kb on - strand, within EX31_RS12015 at 67.485 kb on - strand, within EX31_RS12015 at 67.539 kb on + strand, within EX31_RS12015 at 67.539 kb on + strand, within EX31_RS12015 at 67.539 kb on + strand, within EX31_RS12015 at 67.595 kb on - strand, within EX31_RS12015
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 65,063 + EX31_RS12005 0.19 +0.2 65,064 - EX31_RS12005 0.19 -0.4 65,124 + EX31_RS12005 0.26 -0.1 65,124 + EX31_RS12005 0.26 +0.8 65,131 + EX31_RS12005 0.27 -0.4 65,140 + EX31_RS12005 0.28 -0.0 65,140 + EX31_RS12005 0.28 +0.3 65,141 - EX31_RS12005 0.28 +0.6 65,141 - EX31_RS12005 0.28 -0.9 65,141 - EX31_RS12005 0.28 -1.2 65,141 - EX31_RS12005 0.28 -0.2 65,182 - EX31_RS12005 0.33 +1.5 65,182 - EX31_RS12005 0.33 -0.7 65,390 - EX31_RS12005 0.57 +0.4 65,469 - EX31_RS12005 0.66 +0.9 65,469 - EX31_RS12005 0.66 -2.0 65,661 + EX31_RS12005 0.89 -0.4 65,661 + EX31_RS12005 0.89 -1.3 65,661 + EX31_RS12005 0.89 -0.9 65,662 - EX31_RS12005 0.89 -1.4 65,662 - EX31_RS12005 0.89 +0.5 65,662 - EX31_RS12005 0.89 -1.4 65,662 - EX31_RS12005 0.89 -0.5 65,683 + -0.3 65,684 - +0.7 65,716 + +0.9 65,716 + +0.4 65,717 - -1.6 65,799 - +0.1 65,807 - -0.1 65,807 - +0.2 65,872 - -0.5 65,885 + -0.9 65,995 + -0.4 65,997 + -0.2 66,006 - +1.2 66,008 - +0.6 66,008 - +0.1 66,009 + +0.4 66,012 + -1.3 66,101 - EX31_RS12010 0.20 -0.2 66,128 - EX31_RS12010 0.24 +0.1 66,169 + EX31_RS12010 0.30 +0.9 66,170 - EX31_RS12010 0.30 -0.2 66,170 - EX31_RS12010 0.30 +0.3 66,208 + EX31_RS12010 0.36 -0.2 66,208 + EX31_RS12010 0.36 +0.9 66,209 - EX31_RS12010 0.36 -0.2 66,209 - EX31_RS12010 0.36 +0.3 66,228 + EX31_RS12010 0.39 -0.4 66,228 + EX31_RS12010 0.39 +0.3 66,467 + EX31_RS12010 0.73 +0.3 66,467 + EX31_RS12010 0.73 +1.0 66,467 + EX31_RS12010 0.73 +0.2 66,547 - EX31_RS12010 0.85 +0.4 66,609 + +0.2 66,716 + +0.1 66,716 + -0.7 66,865 + +0.3 66,866 - +1.5 66,890 - -0.5 66,935 + +0.7 66,994 - +0.0 66,994 - -1.1 67,048 + +0.2 67,048 + -0.3 67,049 - +1.2 67,050 + +0.4 67,050 + +1.9 67,051 - -0.8 67,051 - -0.5 67,051 - -0.1 67,051 - +0.9 67,062 + +1.3 67,062 + +0.7 67,134 + -1.1 67,135 - -0.6 67,135 - -0.8 67,138 + -1.3 67,138 + -0.4 67,160 + -0.2 67,160 + -1.6 67,223 + -0.4 67,223 + -0.5 67,224 - +1.1 67,272 + -0.3 67,273 - -0.5 67,273 - +0.9 67,279 + -0.6 67,280 - -0.4 67,301 - +0.6 67,301 - +0.1 67,306 + -0.3 67,306 + +0.8 67,306 + -0.5 67,306 + +0.4 67,307 - -0.0 67,307 - -1.2 67,341 - +0.8 67,350 + -0.5 67,350 + -0.3 67,351 - -1.0 67,351 - -1.5 67,351 - +1.0 67,351 - +0.3 67,484 + EX31_RS12015 0.14 +0.4 67,484 + EX31_RS12015 0.14 +0.7 67,484 + EX31_RS12015 0.14 -0.1 67,485 - EX31_RS12015 0.14 -0.1 67,485 - EX31_RS12015 0.14 +0.6 67,485 - EX31_RS12015 0.14 -0.2 67,485 - EX31_RS12015 0.14 -0.2 67,485 - EX31_RS12015 0.14 +1.8 67,485 - EX31_RS12015 0.14 +1.1 67,539 + EX31_RS12015 0.17 +1.0 67,539 + EX31_RS12015 0.17 +1.8 67,539 + EX31_RS12015 0.17 +0.7 67,595 - EX31_RS12015 0.21 +0.0
Or see this region's nucleotide sequence